{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:48:04Z","timestamp":1790826484124,"version":"4.1.0"},"reference-count":51,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100004412","name":"T\u00fcrkiye Bilimler Akademisi","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2023,11]]},"DOI":"10.1016\/j.toxicon.2023.107328","type":"journal-article","created":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T02:37:30Z","timestamp":1698201450000},"page":"107328","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"special_numbering":"C","title":["Composition characterization of various viperidae snake venoms using MS-based proteomics N-glycoproteomics and N-glycomics"],"prefix":"10.1016","volume":"235","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5623-7363","authenticated-orcid":false,"given":"Marwa","family":"Abu Aisheh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haci Mehmet","family":"Kayili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5818-7881","authenticated-orcid":false,"given":"Yasemin","family":"Numanoglu Cevik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3494-4563","authenticated-orcid":false,"given":"Mehmet Ali","family":"Kanat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8542-6531","authenticated-orcid":false,"given":"Bekir","family":"Salih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2023.107328_bib1","doi-asserted-by":"crossref","first-page":"90","DOI":"10.3390\/toxins11020090","article-title":"Vipera berus berus venom from Russia: venomics, bioactivities and preclinical assessment of microgen antivenom","volume":"11","author":"Al-Shekhadat","year":"2019","journal-title":"Toxins"},{"key":"10.1016\/j.toxicon.2023.107328_bib2","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1074\/mcp.RA118.000748","article-title":"Structures of N-glycans of Bothrops venoms revealed as molecular signatures that contribute to venom phenotype in viperid snakes","volume":"17","author":"Andrade-Silva","year":"2018","journal-title":"Mol. Cell. Proteomics"},{"key":"10.1016\/j.toxicon.2023.107328_bib3","doi-asserted-by":"crossref","first-page":"2658","DOI":"10.1021\/acs.jproteome.6b00217","article-title":"Proteomic and glycoproteomic profilings reveal that post-translational modifications of toxins contribute to venom phenotype in snakes","volume":"15","author":"Andrade-Silva","year":"2016","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.toxicon.2023.107328_bib4","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1021\/acs.jproteome.0c00737","article-title":"Venom profiling of the insular species Bothrops alcatraz: characterization of proteome, glycoproteome, and N-terminome using terminal amine isotopic labeling of substrates","volume":"20","author":"Andrade-Silva","year":"2021","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.toxicon.2023.107328_bib5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.11646\/zootaxa.1038.1.1","article-title":"An electrophoretic comparison of the venoms of a colubrid and various viperid snakes from Turkey and Cyprus, with some taxonomic and phylogenetic implications","volume":"1038","author":"Ar\u0131kan","year":"2005","journal-title":"Zootaxa"},{"key":"10.1016\/j.toxicon.2023.107328_bib6","doi-asserted-by":"crossref","first-page":"4223","DOI":"10.1002\/pmic.200402024","article-title":"Snake venomics: comparative analysis of the venom proteomes of the Tunisian snakes Cerastes cerastes, Cerastes vipera and Macrovipera lebetina","volume":"5","author":"Bazaa","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.toxicon.2023.107328_bib7","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.3390\/molecules21101398","article-title":"Proteome and peptidome of Vipera berus berus venom","volume":"21","author":"Bocian","year":"2016","journal-title":"Molecules"},{"key":"10.1016\/j.toxicon.2023.107328_bib8","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/j.bbagen.2016.12.022","article-title":"Minor snake venom proteins: structure, function and potential applications","volume":"1861","author":"Boldrini-Franca","year":"2017","journal-title":"Biochim. Biophys. Acta Gen. Subj."},{"key":"10.1016\/j.toxicon.2023.107328_bib9","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.biochi.2022.09.011","article-title":"Sialic acid-containing glycans play a role in the activity of snake venom proteases","volume":"204","author":"Br\u00e1s-Costa","year":"2023","journal-title":"Biochimie"},{"key":"10.1016\/j.toxicon.2023.107328_bib10","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1590\/S1678-91992010000400007","article-title":"Snakebites in Turkey: epidemiological and clinical aspects between the years 1995 and 2004","volume":"16","author":"Cesaretli","year":"2010","journal-title":"J. Venom. Anim. Toxins Incl. Trop. Dis."},{"key":"10.1016\/j.toxicon.2023.107328_bib11","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.toxicon.2011.10.008","article-title":"Epidemiology of snakebites in Europe: a systematic review of the literature","volume":"59","author":"Chippaux","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2023.107328_bib12","doi-asserted-by":"crossref","DOI":"10.3390\/toxins13060427","article-title":"Old world vipers-A review about snake venom proteomics of Viperinae and their variations","volume":"13","author":"Damm","year":"2021","journal-title":"Toxins"},{"key":"10.1016\/j.toxicon.2023.107328_bib13","doi-asserted-by":"crossref","DOI":"10.1016\/j.tox.2021.152724","article-title":"Vipers of Major clinical relevance in Europe: taxonomy, venom composition, toxicology and clinical management of human bites","volume":"453","author":"Di Nicola","year":"2021","journal-title":"Toxicology"},{"key":"10.1016\/j.toxicon.2023.107328_bib14","first-page":"1","article-title":"Venomous snake bite in Turkey","volume":"1","author":"Ertem","year":"2004","journal-title":"Eur. J. Gen. Med."},{"key":"10.1016\/j.toxicon.2023.107328_bib15","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1163\/15685381-bja10007","article-title":"Evaluating taxonomic inflation: towards evidence-based species delimitation in Eurasian vipers (Serpentes: Viperinae)","volume":"41","author":"Freitas","year":"2020","journal-title":"Amphibia-Reptilia"},{"key":"10.1016\/j.toxicon.2023.107328_bib16","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1021\/pr070376c","article-title":"Comparative analysis of the venom proteomes of Vipera ammodytes ammodytes and Vipera ammodytes meridionalis","volume":"7","author":"Georgieva","year":"2008","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.toxicon.2023.107328_bib17","article-title":"Study of the venom proteome of Vipera ammodytes ammodytes (Linnaeus, 1758): a qualitative overview, biochemical and biological profiling","volume":"37","author":"Gopcevic","year":"2021","journal-title":"Comp. Biochem. Physiol., Part D: Genomics Proteomics"},{"key":"10.1016\/j.toxicon.2023.107328_bib18","doi-asserted-by":"crossref","first-page":"10644","DOI":"10.1016\/S0021-9258(17)34108-X","article-title":"Factor X-activating glycoprotein of Russell's viper venom. Polypeptide composition and characterization of the carbohydrate moieties","volume":"269","author":"Gowda","year":"1994","journal-title":"J. Biol. Chem."},{"key":"10.1016\/j.toxicon.2023.107328_bib19","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.toxicon.2012.05.001","article-title":"Past decade study of snake venom L-amino acid oxidase","volume":"60","author":"Guo","year":"2012","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2023.107328_bib20","article-title":"Snakebite envenoming","volume":"3","author":"Gutierrez","year":"2017","journal-title":"Nat. Rev. Dis. Prim."},{"key":"10.1016\/j.toxicon.2023.107328_bib21","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1186\/s12859-015-0611-3","article-title":"InteractiVenn: a web-based tool for the analysis of sets through Venn diagrams","volume":"16","author":"Heberle","year":"2015","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.toxicon.2023.107328_bib22","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3390\/toxins10010023","article-title":"Comparative venomics of the Vipera ammodytes transcaucasiana and Vipera ammodytes montandoni from Turkey provides insights into kinship","volume":"10","author":"Hempel","year":"2018","journal-title":"Toxins"},{"key":"10.1016\/j.toxicon.2023.107328_bib23","doi-asserted-by":"crossref","first-page":"1731","DOI":"10.1021\/acs.jproteome.9b00869","article-title":"Extended snake venomics by top-down in-source decay: investigating the newly discovered anatolian meadow viper subspecies, Vipera anatolica senliki","volume":"19","author":"Hempel","year":"2020","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.toxicon.2023.107328_bib24","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/s00204-011-0763-5","volume":"86","author":"Igci","year":"2012","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.toxicon.2023.107328_bib25","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1007\/s00204-011-0763-5","volume":"86","author":"Igci","year":"2012","journal-title":"Arch. Toxicol."},{"key":"10.1016\/j.toxicon.2023.107328_bib26","doi-asserted-by":"crossref","first-page":"375","DOI":"10.3390\/toxins14060375","article-title":"The phospholipase activity of Ammodytoxin, a prototype snake venom \u03b2-neurotoxin, is not obligatory for cell internalisation and translocation to mitochondria","volume":"14","author":"Ivanu\u0161ec","year":"2022","journal-title":"Toxins"},{"key":"10.1016\/j.toxicon.2023.107328_bib27","series-title":"N-Glycan Profiling of Glycoproteins by Hydrophilic Interaction Liquid Chromatography with Fluorescence and Mass Spectrometric Detection","author":"Kayili","year":"2021"},{"key":"10.1016\/j.toxicon.2023.107328_bib28","article-title":"Site-specific N-glycosylation analysis of human thyroid thyroglobulin by mass spectrometry-based Glyco-analytical strategies","volume":"267","author":"Kayili","year":"2022","journal-title":"J. Proteonomics"},{"key":"10.1016\/j.toxicon.2023.107328_bib29","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.jprot.2016.06.020","article-title":"Venomics of Vipera berus berus to explain differences in pathology elicited by Vipera ammodytes ammodytes envenomation: therapeutic implications","volume":"146","author":"Latinovi\u0107","year":"2016","journal-title":"J. Proteonomics"},{"key":"10.1016\/j.toxicon.2023.107328_bib30","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1021\/acs.jproteome.9b00120","article-title":"Comprehensive study of the proteome and transcriptome of the venom of the most venomous European viper: discovery of a New subclass of ancestral snake venom metalloproteinase precursor-derived proteins","volume":"18","author":"Leonardi","year":"2019","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.toxicon.2023.107328_bib31","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.toxicon.2012.09.008","article-title":"Non-enzymatic proteins from snake venoms: a gold mine of pharmacological tools and drug leads","volume":"62","author":"McCleary","year":"2013","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2023.107328_bib32","doi-asserted-by":"crossref","first-page":"564","DOI":"10.3390\/toxins11100564","article-title":"Snake venoms in drug discovery: valuable therapeutic tools for life saving","volume":"11","author":"Mohamed Abd El-Aziz","year":"2019","journal-title":"Toxins"},{"key":"10.1016\/j.toxicon.2023.107328_bib33","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1016\/j.toxicon.2005.02.021","article-title":"Structures and functions of snake venom CLPs (C-type lectin-like proteins) with anticoagulant-, procoagulant-, and platelet-modulating activities","volume":"45","author":"Morita","year":"2005","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2023.107328_bib34","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.toxicon.2017.06.008","article-title":"Combined venom profiling and cytotoxicity screening of the Radde's mountain viper (Montivipera raddei) and Mount Bulgar Viper (Montivipera bulgardaghica) with potent cytotoxicity against human A549 lung carcinoma cells","volume":"135","author":"Nalbantsoy","year":"2017","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2023.107328_bib35","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1021\/pr034052+","article-title":"Analysis of lectin-bound glycoproteins in snake venom from the elapidae and Viperidae families","volume":"3","author":"Nawarak","year":"2004","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.toxicon.2023.107328_bib36","doi-asserted-by":"crossref","first-page":"2838","DOI":"10.1002\/elps.200305552","article-title":"Proteomics of snake venoms from Elapidae and Viperidae families by multidimensional chromatographic methods","volume":"24","author":"Nawarak","year":"2003","journal-title":"Electrophoresis"},{"key":"10.1016\/j.toxicon.2023.107328_bib37","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1038\/s41570-022-00393-7","article-title":"The chemistry of snake venom and its medicinal potential","volume":"6","author":"Oliveira","year":"2022","journal-title":"Nat. Rev. Chem"},{"key":"10.1016\/j.toxicon.2023.107328_bib38","doi-asserted-by":"crossref","first-page":"2597","DOI":"10.1002\/pmic.201400515","article-title":"FunRich: an open access standalone functional enrichment and interaction network analysis tool","volume":"15","author":"Pathan","year":"2015","journal-title":"Proteomics"},{"key":"10.1016\/j.toxicon.2023.107328_bib39","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1038\/s41592-020-0967-9","article-title":"Fast and comprehensive N- and O-glycoproteomics analysis with MSFragger-Glyco","volume":"17","author":"Polasky","year":"2020","journal-title":"Nat. Methods"},{"key":"10.1016\/j.toxicon.2023.107328_bib40","doi-asserted-by":"crossref","first-page":"1896","DOI":"10.1038\/nprot.2007.261","article-title":"Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips","volume":"2","author":"Rappsilber","year":"2007","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.toxicon.2023.107328_bib41","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1136\/emj.13.5.346","article-title":"Incidence, pathology, and treatment of adder (Vipera berus L.) bites in man","volume":"13","author":"Reading","year":"1996","journal-title":"Emerg. Med. J."},{"key":"10.1016\/j.toxicon.2023.107328_bib42","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0041-0101(01)00187-8","article-title":"Biochemical characterization of fibrinogenolytic serine proteinases from Vipera lebetina snake venom","volume":"40","author":"Samel","year":"2002","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2023.107328_bib43","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1016\/j.jprot.2008.05.003","article-title":"Snake venomics of the Armenian mountain vipers Macrovipera lebetina obtusa and Vipera raddei","volume":"71","author":"Sanz","year":"2008","journal-title":"J. Proteonomics"},{"key":"10.1016\/j.toxicon.2023.107328_bib44","doi-asserted-by":"crossref","first-page":"2492","DOI":"10.1021\/ac1027116","article-title":"Cotton HILIC SPE microtips for microscale purification and enrichment of glycans and glycopeptides","volume":"83","author":"Selman","year":"2011","journal-title":"Anal. Chem."},{"key":"10.1016\/j.toxicon.2023.107328_bib45","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1002\/pmic.200400931","article-title":"A multifaceted analysis of viperid snake venoms by two\u2010dimensional gel electrophoresis: an approach to understanding venom proteomics","volume":"5","author":"Serrano","year":"2005","journal-title":"Proteomics"},{"key":"10.1016\/j.toxicon.2023.107328_bib46","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/0041-0101(76)90088-X","article-title":"Pharmacological study of phospholipase A from Vipera ammodytes venom","volume":"14","author":"Sket","year":"1976","journal-title":"Toxicon"},{"key":"10.1016\/j.toxicon.2023.107328_bib47","doi-asserted-by":"crossref","first-page":"913","DOI":"10.2174\/092986609788923293","article-title":"Venom-sweet-venom: N-linked glycosylation in snake venom toxins","volume":"16","author":"Soares","year":"2009","journal-title":"Protein Pept. Lett."},{"key":"10.1016\/j.toxicon.2023.107328_bib48","doi-asserted-by":"crossref","first-page":"290","DOI":"10.3390\/toxins9090290","article-title":"A review and database of snake venom proteomes","volume":"9","author":"Tasoulis","year":"2017","journal-title":"Toxins"},{"key":"10.1016\/j.toxicon.2023.107328_bib49","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1038\/nmeth.3901","article-title":"The Perseus computational platform for comprehensive analysis of (prote)omics data","volume":"13","author":"Tyanova","year":"2016","journal-title":"Nat. Methods"},{"key":"10.1016\/j.toxicon.2023.107328_bib50","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0140-6736(09)61754-2","article-title":"Snake bite","volume":"375","author":"Warrell","year":"2010","journal-title":"Lancet"},{"key":"10.1016\/j.toxicon.2023.107328_bib51","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s10616-013-9540-z","article-title":"Effect of Ottoman viper (Montivipera xanthina (Gray, 1849)) venom on various cancer cells and on microorganisms","volume":"66","author":"Yalc\u0131n","year":"2014","journal-title":"Cytotechnology"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010123003148?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041010123003148?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T10:23:31Z","timestamp":1777458211000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041010123003148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11]]},"references-count":51,"alternative-id":["S0041010123003148"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2023.107328","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2023,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Composition characterization of various viperidae snake venoms using MS-based proteomics N-glycoproteomics and N-glycomics","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2023.107328","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2023 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"107328"}}