{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,11]],"date-time":"2026-01-11T14:44:19Z","timestamp":1768142659944,"version":"3.49.0"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T00:00:00Z","timestamp":1717200000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100014786","name":"Northern Border University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014786","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicon"],"published-print":{"date-parts":[[2024,6]]},"DOI":"10.1016\/j.toxicon.2024.107754","type":"journal-article","created":{"date-parts":[[2024,5,16]],"date-time":"2024-05-16T06:59:54Z","timestamp":1715842794000},"page":"107754","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":3,"special_numbering":"C","title":["Nigella sativa, active principle thymoquinone, alleviates palmitate-induced cytotoxicity, inflammation and bioenergetic disruptions in macrophages: An invitro study model"],"prefix":"10.1016","volume":"244","author":[{"given":"Rashad","family":"Othman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amal","family":"Aggour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7444-2499","authenticated-orcid":false,"given":"Ekramy","family":"Elmorsy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1252-8403","authenticated-orcid":false,"given":"Manal S.","family":"Fawzy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxicon.2024.107754_bib1","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0378-8741(01)00216-1","article-title":"The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa","volume":"76","author":"Al-Ghamdi","year":"2001","journal-title":"J. Ethnopharmacol."},{"key":"10.1016\/j.toxicon.2024.107754_bib2","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.biopha.2018.01.028","article-title":"Thymoquinone reduces mortality and suppresses early acute inflammatory markers of sepsis in a mouse model","volume":"98","author":"Alkharfy","year":"2018","journal-title":"Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie"},{"key":"10.1016\/j.toxicon.2024.107754_bib3","doi-asserted-by":"crossref","first-page":"5240","DOI":"10.3390\/nu14245240","article-title":"Thymoquinone effect on monocyte-derived macrophages, cell-surface molecule expression, and phagocytosis","volume":"14","author":"Alkhattabi","year":"2022","journal-title":"Nutrients"},{"key":"10.1016\/j.toxicon.2024.107754_bib4","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.gendis.2019.07.010","article-title":"Recent advances in chronic granulomatous disease","volume":"7","author":"Anjani","year":"2020","journal-title":"Genes & diseases"},{"key":"10.1016\/j.toxicon.2024.107754_bib5","doi-asserted-by":"crossref","first-page":"327","DOI":"10.2174\/1566524023666230410101724","article-title":"Potential role of Nigella sativa and its constituent (thymoquinone) in ischemic stroke","volume":"24","author":"Azami","year":"2024","journal-title":"Curr. Mol. Med."},{"key":"10.1016\/j.toxicon.2024.107754_bib6","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1016\/j.cell.2017.04.004","article-title":"Metabolic instruction of immunity","volume":"169","author":"Buck","year":"2017","journal-title":"Cell"},{"key":"10.1016\/j.toxicon.2024.107754_bib7","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/s41392-023-01452-1","article-title":"Macrophages in immunoregulation and therapeutics","volume":"8","author":"Chen","year":"2023","journal-title":"Signal Transduct Target Ther"},{"key":"10.1016\/j.toxicon.2024.107754_bib8","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0003-2697(82)90118-X","article-title":"Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids","volume":"126","author":"Green","year":"1982","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.toxicon.2024.107754_bib9","doi-asserted-by":"crossref","DOI":"10.1038\/srep42995","article-title":"Thymoquinone: an IRAK1 inhibitor with in vivo and in vitro anti-inflammatory activities","volume":"7","author":"Hossen","year":"2017","journal-title":"Sci. Rep."},{"key":"10.1016\/j.toxicon.2024.107754_bib46","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1373\/clinchem.2006.078873","article-title":"Spectrophotometric assay for complex I of the respiratory chain in tissue samples and cultured fibroblasts","volume":"53","author":"Janssen","year":"2007","journal-title":"Clinical chemistry"},{"key":"10.1016\/j.toxicon.2024.107754_bib10","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.immuni.2015.02.005","article-title":"Network integration of parallel metabolic and transcriptional data reveals metabolic modules that regulate macrophage polarization","volume":"42","author":"Jha","year":"2015","journal-title":"Immunity"},{"key":"10.1016\/j.toxicon.2024.107754_bib11","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.3390\/microorganisms11092233","article-title":"Chronic granulomatous disease (CGD): commonly associated pathogens, diagnosis and treatment","volume":"11","author":"Justiz-Vaillant","year":"2023","journal-title":"Microorganisms"},{"key":"10.1016\/j.toxicon.2024.107754_bib12","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1097\/01.EHX.0000440828.86537.e7","article-title":"Effects of aluminum on rat cerebellar cortex and the possible protective role of Nigella sativa: a light and electron microscopic study","volume":"36","author":"Kamal","year":"2013","journal-title":"Egyptian Journal of Histology"},{"key":"10.1016\/j.toxicon.2024.107754_bib13","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1007\/s10735-010-9305-3","article-title":"Protective effects of thymoquinone on the neuronal injury in frontal cortex after chronic toluene exposure","volume":"42","author":"Kanter","year":"2011","journal-title":"J. Mol. Histol."},{"key":"10.1016\/j.toxicon.2024.107754_bib14","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1007\/s10787-023-01213-0","article-title":"The effect of Nigella sativa (black seed) on biomarkers of inflammation and oxidative stress: an updated systematic review and meta-analysis of randomized controlled trials","volume":"31","author":"Kavyani","year":"2023","journal-title":"Inflammopharmacology"},{"key":"10.1016\/j.toxicon.2024.107754_bib15","doi-asserted-by":"crossref","DOI":"10.2174\/1871525721666221221161742","article-title":"Protective effect of Nigella sativa seed extract and its bioactive compound thymoquinone on streptozotocin-induced diabetic rats","author":"Khan","year":"2024","journal-title":"Cardiovasc. Hematol. Agents Med. Chem. 22, 51-59"},{"key":"10.1016\/j.toxicon.2024.107754_bib16","doi-asserted-by":"crossref","first-page":"2384","DOI":"10.1021\/acs.accounts.2c00159","article-title":"Nitric-oxide-modulatory materials for biomedical applications","volume":"55","author":"Kim","year":"2022","journal-title":"Accounts of Chemical Research"},{"key":"10.1016\/j.toxicon.2024.107754_bib17","doi-asserted-by":"crossref","first-page":"9039","DOI":"10.3390\/cimb45110567","article-title":"How thymoquinone from Nigella sativa accelerates wound healing through multiple mechanisms and targets","volume":"45","author":"Kmail","year":"2023","journal-title":"Curr. Issues Mol. Biol."},{"key":"10.1016\/j.toxicon.2024.107754_bib18","article-title":"Anti-inflammatory effects of thymoquinone and its protective effects against several diseases","volume":"138","author":"Kohandel","year":"2021","journal-title":"Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie"},{"key":"10.1016\/j.toxicon.2024.107754_bib19","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1007\/s00011-019-01273-5","article-title":"The effect of palmitic acid on inflammatory response in macrophages: an overview of molecular mechanisms","volume":"68","author":"Korbecki","year":"2019","journal-title":"Inflamm. Res."},{"key":"10.1016\/j.toxicon.2024.107754_bib20","doi-asserted-by":"crossref","first-page":"13384","DOI":"10.3390\/ijms222413384","article-title":"Mitochondrial oxidative stress-A causative factor and therapeutic target in many diseases","volume":"22","author":"Kowalczyk","year":"2021","journal-title":"Int. J. Mol. Sci."},{"key":"10.1016\/j.toxicon.2024.107754_bib21","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.cmet.2014.08.010","article-title":"Metabolic dysfunction drives a mechanistically distinct pro-inflammatory phenotype in adipose tissue macrophages","volume":"20","author":"Kratz","year":"2014","journal-title":"Cell Metabol."},{"key":"10.1016\/j.toxicon.2024.107754_bib22","doi-asserted-by":"crossref","first-page":"D684","DOI":"10.1093\/nar\/gkm795","article-title":"STITCH: interaction networks of chemicals and proteins","volume":"36","author":"Kuhn","year":"2008","journal-title":"Nucleic acids research"},{"key":"10.1016\/j.toxicon.2024.107754_bib23","doi-asserted-by":"crossref","first-page":"61","DOI":"10.3389\/fimmu.2017.00061","article-title":"Metabolism supports macrophage activation","volume":"8","author":"Langston","year":"2017","journal-title":"Front. Immunol."},{"key":"10.1016\/j.toxicon.2024.107754_bib24","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40364-020-00251-y","article-title":"Metabolic reprogramming in macrophage responses","volume":"9","author":"Liu","year":"2021","journal-title":"Biomark. Res."},{"key":"10.1016\/j.toxicon.2024.107754_bib25","doi-asserted-by":"crossref","DOI":"10.3390\/cells12172124","article-title":"Mitochondria in the spotlight: C. elegans as a model organism to evaluate xenobiotic-induced dysfunction","volume":"12","author":"Martins","year":"2023","journal-title":"Cells"},{"key":"10.1016\/j.toxicon.2024.107754_bib26","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1038\/s41577-023-00848-y","article-title":"Tissue-specific macrophages: how they develop and choreograph tissue biology","volume":"23","author":"Mass","year":"2023","journal-title":"Nat. Rev. Immunol."},{"key":"10.1016\/j.toxicon.2024.107754_bib27","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.cell.2016.08.064","article-title":"Succinate dehydrogenase supports metabolic repurposing of mitochondria to drive inflammatory macrophages","volume":"167","author":"Mills","year":"2016","journal-title":"Cell"},{"key":"10.1016\/j.toxicon.2024.107754_bib28","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.1016\/j.fct.2010.05.072","article-title":"Thymoquinone supplementation reverses acetaminophen-induced oxidative stress, nitric oxide production and energy decline in mice liver","volume":"48","author":"Nagi","year":"2010","journal-title":"Food Chem. Toxicol. : an international journal published for the British Industrial Biological Research Association"},{"key":"10.1016\/j.toxicon.2024.107754_bib29","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1007\/s00125-014-3173-4","article-title":"Inhibition of macrophage fatty acid beta-oxidation exacerbates palmitate-induced inflammatory and endoplasmic reticulum stress responses","volume":"57","author":"Namgaladze","year":"2014","journal-title":"Diabetologia"},{"key":"10.1016\/j.toxicon.2024.107754_bib30","doi-asserted-by":"crossref","first-page":"607","DOI":"10.3390\/antiox12030607","article-title":"Antioxidant properties of thymoquinone, thymohydroquinone and black cumin (Nigella sativa L.) seed oil: scavenging of superoxide radical studied using cyclic voltammetry, DFT and single crystal X-ray diffraction","volume":"12","author":"Sakib","year":"2023","journal-title":"Antioxidants"},{"key":"10.1016\/j.toxicon.2024.107754_bib31","article-title":"Anticancer activity of thymoquinone against breast cancer cells: mechanisms of action and delivery approaches","volume":"165","author":"Shabani","year":"2023","journal-title":"Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie"},{"key":"10.1016\/j.toxicon.2024.107754_bib32","doi-asserted-by":"crossref","first-page":"L105","DOI":"10.1152\/ajplung.00470.2006","article-title":"Pro-inflammatory response of alveolar epithelial cells is enhanced by alveolar macrophage-produced TNF-alpha during pulmonary ischemia-reperfusion injury","volume":"293","author":"Sharma","year":"2007","journal-title":"Am. J. Physiol. Lung Cell Mol. Physiol."},{"key":"10.1016\/j.toxicon.2024.107754_bib33","doi-asserted-by":"crossref","first-page":"52","DOI":"10.2174\/1871529X18666180212114816","article-title":"Immunomodulatory and anti-inflammatory effects of thymoquinone","volume":"18","author":"Shaterzadeh-Yazdi","year":"2018","journal-title":"Cardiovascular & hematological disorders drug targets"},{"key":"10.1016\/j.toxicon.2024.107754_bib34","doi-asserted-by":"crossref","DOI":"10.3389\/fnut.2023.1126272","article-title":"Mechanism of the antidiabetic action of Nigella sativa and Thymoquinone: a review","volume":"10","author":"Shaukat","year":"2023","journal-title":"Front. Nutr."},{"key":"10.1016\/j.toxicon.2024.107754_bib47","doi-asserted-by":"crossref","first-page":"1235","DOI":"10.1038\/nprot.2012.058","article-title":"Assessment of mitochondrial respiratory chain enzymatic activities on tissues and cultured cells","volume":"7","author":"Spinazzi","year":"2012","journal-title":"Nature protocols"},{"key":"10.1016\/j.toxicon.2024.107754_bib35","article-title":"Effects of metabolism on macrophage polarization under different disease backgrounds","volume":"13","author":"Sun","year":"2022","journal-title":"Front. Immunol."},{"key":"10.1016\/j.toxicon.2024.107754_bib36","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1038\/nature11986","article-title":"Succinate is an inflammatory signal that induces IL-1beta through HIF-1alpha","volume":"496","author":"Tannahill","year":"2013","journal-title":"Nature"},{"key":"10.1016\/j.toxicon.2024.107754_bib37","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2022.832356","article-title":"Mitochondria related cell death modalities and disease","volume":"10","author":"Tian","year":"2022","journal-title":"Front. Cell Dev. Biol."},{"key":"10.1016\/j.toxicon.2024.107754_bib38","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.ai.2016.12.001","article-title":"Macrophages and mitochondria: a critical interplay between metabolism, signaling, and the functional activity","volume":"133","author":"Tur","year":"2017","journal-title":"Adv. Immunol."},{"key":"10.1016\/j.toxicon.2024.107754_bib39","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/1471-2202-13-11","article-title":"Neuroprotection with metformin and thymoquinone against ethanol-induced apoptotic neurodegeneration in prenatal rat cortical neurons","volume":"13","author":"Ullah","year":"2012","journal-title":"BMC Neurosci."},{"key":"10.1016\/j.toxicon.2024.107754_bib40","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/j.cbi.2012.03.003","article-title":"Modulation of the oxidative stress and inflammatory cytokine response by thymoquinone in the collagen induced arthritis in Wistar rats","volume":"197","author":"Umar","year":"2012","journal-title":"Chem. Biol. Interact."},{"key":"10.1016\/j.toxicon.2024.107754_bib41","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.cmet.2006.05.011","article-title":"Oxidative metabolism and PGC-1beta attenuate macrophage-mediated inflammation","volume":"4","author":"Vats","year":"2006","journal-title":"Cell Metabol."},{"key":"10.1016\/j.toxicon.2024.107754_bib42","doi-asserted-by":"crossref","first-page":"3829","DOI":"10.3390\/plants12223829","article-title":"Potential pharmacological applications of Nigella seeds with a focus on Nigella sativa and its constituents against chronic inflammatory diseases: progress and future opportunities","volume":"12","author":"Wahab","year":"2023","journal-title":"Plants"},{"key":"10.1016\/j.toxicon.2024.107754_bib43","doi-asserted-by":"crossref","DOI":"10.3389\/fnut.2022.899797","article-title":"The immunomodulatory effects of active ingredients from Nigella sativa in RAW264.7 cells through NF-kappaB\/MAPK signaling pathways","volume":"9","author":"Wei","year":"2022","journal-title":"Front. Nutr."},{"key":"10.1016\/j.toxicon.2024.107754_bib44","first-page":"5","article-title":"Medicinal plants growing in our republic medicinal properties","volume":"15","author":"Yusupova","year":"2023","journal-title":"Periodica Journal of Modern Philosophy, Social Sciences and Humanities"},{"key":"10.1016\/j.toxicon.2024.107754_bib45","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1016\/j.cell.2015.12.057","article-title":"NF-kappaB restricts inflammasome activation via elimination of damaged mitochondria","volume":"164","author":"Zhong","year":"2016","journal-title":"Cell"}],"container-title":["Toxicon"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004101012400326X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S004101012400326X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T04:54:21Z","timestamp":1731992061000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S004101012400326X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6]]},"references-count":47,"alternative-id":["S004101012400326X"],"URL":"https:\/\/doi.org\/10.1016\/j.toxicon.2024.107754","relation":{},"ISSN":["0041-0101"],"issn-type":[{"value":"0041-0101","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nigella sativa, active principle thymoquinone, alleviates palmitate-induced cytotoxicity, inflammation and bioenergetic disruptions in macrophages: An invitro study model","name":"articletitle","label":"Article Title"},{"value":"Toxicon","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxicon.2024.107754","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2024 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"107754"}}