{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:53:03Z","timestamp":1788580383352,"version":"build-2803163510"},"reference-count":40,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["M1704166"],"award-info":[{"award-number":["M1704166"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2016M592314"],"award-info":[{"award-number":["2016M592314"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Foundation of Henan Province Scientific and Technological Open Cooperation Projects","award":["182106000061"],"award-info":[{"award-number":["182106000061"]}]},{"name":"Department of Education of Henan Province Foundation","award":["17A310006"],"award-info":[{"award-number":["17A310006"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Toxicology Letters"],"published-print":{"date-parts":[[2019,11]]},"DOI":"10.1016\/j.toxlet.2019.08.020","type":"journal-article","created":{"date-parts":[[2019,8,30]],"date-time":"2019-08-30T11:38:38Z","timestamp":1567165118000},"page":"10-19","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":5,"special_numbering":"C","title":["Establishment of rapid risk assessment model for cigarette smoke extract exposure in chronic obstructive pulmonary disease"],"prefix":"10.1016","volume":"316","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5614-6859","authenticated-orcid":false,"given":"Xuemei","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenfeng","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peijun","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinpei","family":"Xing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohan","family":"xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyi","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junxia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangjun","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huimin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0724-0982","authenticated-orcid":false,"given":"Xiang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuwei","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.toxlet.2019.08.020_bib0005","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0166255","article-title":"Cigarette smoke modulates repair and innate immunity following injury to airway epithelial cells","volume":"11","author":"Amatngalim","year":"2016","journal-title":"PLoS. One."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0010","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1056\/NEJM200007273430407","article-title":"Chronic obstructive pulmonary disease","volume":"343","author":"Barnes","year":"2000","journal-title":"N.Engl. J. Med."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0015","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1080\/01902148.2017.1303098","article-title":"Functional characterization of a novel 3D model of the epithelial-mesenchymal trophic unit","volume":"43","author":"Bucchieri","year":"2017","journal-title":"Exp. Lung Res."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0020","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.toxlet.2017.01.005","article-title":"Inhibition of monoamine oxidase-B by selegiline reduces cigarette smoke-induced oxidative stress and inflammation in airway epithelial cells","volume":"268","author":"Cui","year":"2017","journal-title":"Toxicol. Lett."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0025","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1016\/S0955-0674(02)00364-2","article-title":"Cell interactions with three-dimensional matrices","volume":"14","author":"Cukierman","year":"2002","journal-title":"Curr. Opin. Cell Biol."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0030","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1080\/17476348.2017.1360769","article-title":"Airway inflammation in chronic obstructive pulmonary disease (COPD): a true paradox","volume":"11","author":"Eapen","year":"2017","journal-title":"Expert Rev. Respir. Med."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0035","article-title":"Hematoxylin and eosin staining of tissue and cell sections","author":"Fischer","year":"2008","journal-title":"CSH Protoc."},{"issue":"18","key":"10.1016\/j.toxlet.2019.08.020_bib0040","article-title":"Repeated exposure to streptococcus pneumoniae exacerbates chronic obstructive pulmonary disease","volume":"S0002\u20139440","author":"Gou","year":"2019","journal-title":"Am. J.Pathol."},{"issue":"1","key":"10.1016\/j.toxlet.2019.08.020_bib0045","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1186\/s13104-018-3309-6","article-title":"Chronic obstructive pulmonary disease (COPD): neutrophils, macrophages and lymphocytes in patients with anterior tuberculosis compared to tobacco related COPD","volume":"11","author":"Guiedem","year":"2018","journal-title":"BMC Res. Notes"},{"key":"10.1016\/j.toxlet.2019.08.020_bib0050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-60761-984-0_1","article-title":"3D cell culture: a review of current approaches and techniques","volume":"695","author":"Haycock","year":"2011","journal-title":"Methods Mol. Biol."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0055","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.2147\/COPD.S147144","article-title":"Characteristics and potential role of M2 macrophages in COPD","volume":"12","author":"He","year":"2017","journal-title":"Int. J. Chron. Obstruct. Pulmon. Dis."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0060","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1183\/09031936.00141514","article-title":"The innate immune function of airway epithelial cells in inflammatory lung disease","volume":"45","author":"Hiemstra","year":"2015","journal-title":"Eur. Respir. J."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0065","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1289\/ehp.5519","article-title":"Effects of submicrometer particle compositions on cytokine production and lipid peroxidation of human bronchial epithelial cells","volume":"111","author":"Huang","year":"2003","journal-title":"Environ. Health Perspect."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0070","doi-asserted-by":"crossref","first-page":"1837","DOI":"10.3892\/or.2015.3767","article-title":"Comparison of 2D- and 3D-culture models as drug-testing platforms in breast cancer","volume":"33","author":"Imamura","year":"2015","journal-title":"Oncol. Rep."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0075","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1097\/MCP.0000000000000145","article-title":"The chronic bronchitis phenotype in chronic obstructive pulmonary disease: features and implications","volume":"21","author":"Kim","year":"2015","journal-title":"Curr. Opin. Pulm. Med."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0080","doi-asserted-by":"crossref","DOI":"10.1242\/bio.040808","article-title":"Microenvironmental stimuli induce different macrophage polarizations in experimental models of emphysema","volume":"8","author":"Kohler","year":"2019","journal-title":"Biol. Open"},{"key":"10.1016\/j.toxlet.2019.08.020_bib0085","first-page":"153","article-title":"Elevated levels of soluble adhesion molecules in sera of patients with acute bronchiolitis","volume":"37","author":"Lai","year":"2004","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0090","doi-asserted-by":"crossref","first-page":"5212","DOI":"10.1172\/JCI69636","article-title":"Histone deacetylase 6-mediated selective autophagy regulates COPD-associated cilia dysfunction","volume":"123","author":"Lam","year":"2013","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0095","doi-asserted-by":"crossref","DOI":"10.1088\/1748-605X\/aa8357","article-title":"Mechanical properties and cell-culture characteristics of a polycaprolactone kagome-structure scaffold fabricated by a precision extruding deposition system","volume":"12","author":"Lee","year":"2017","journal-title":"Biomed. Mater."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0100","doi-asserted-by":"crossref","first-page":"970","DOI":"10.3389\/fphar.2018.00970","article-title":"Lignans isolated from flower buds of magnolia fargesii attenuate airway inflammation induced by cigarette smoke in vitro and in vivo","volume":"9","author":"Lee","year":"2018","journal-title":"Front. Pharmacol."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0105","doi-asserted-by":"crossref","first-page":"e8157","DOI":"10.1371\/journal.pone.0008157","article-title":"Smoking is associated with shortened airway cilia","volume":"4","author":"Leopold","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.toxlet.2019.08.020_bib0110","doi-asserted-by":"crossref","first-page":"240","DOI":"10.3109\/15376516.2012.743640","article-title":"Evaluation of the cytotoxicity of cigarette smoke total particulate matter using three in vitro assays and two types of cells","volume":"23","author":"Li","year":"2013","journal-title":"Toxicol. Mech. Methods"},{"key":"10.1016\/j.toxlet.2019.08.020_bib0115","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1016\/j.fct.2011.12.008","article-title":"Effects of smoking regimens and test material format on the cytotoxicity of mainstream cigarette smoke","volume":"50","author":"Li","year":"2012","journal-title":"Food Chem. Toxicol."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0120","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1007\/s12031-017-0883-3","article-title":"Small interfering RNA targeting Dickkopf-1 contributes to neuroprotection after intracerebral hemorrhage in rats","volume":"61","author":"Li","year":"2017","journal-title":"J. Mol. Neurosci."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0125","doi-asserted-by":"crossref","first-page":"e4946","DOI":"10.1371\/journal.pone.0004946","article-title":"Effect of cigarette smoke extract on dendritic cells and their impact on T-cell proliferation","volume":"4","author":"Mortaz","year":"2009","journal-title":"PLoS One"},{"key":"10.1016\/j.toxlet.2019.08.020_bib0130","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1016\/j.pupt.2013.03.002","article-title":"IL-8 production in response to cigarette smoke is decreased in epithelial cells from COPD patients","volume":"26","author":"Nadigel","year":"2013","journal-title":"Pulm. Pharmacol. Ther."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0135","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1016\/j.tiv.2015.05.018","article-title":"Development of an in vitro cytotoxicity model for aerosol exposure using 3D reconstructed human airway tissue; application for assessment of e-cigarette aerosol","volume":"29","author":"Neilson","year":"2015","journal-title":"Toxicol. In. Vitro."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0140","doi-asserted-by":"crossref","first-page":"5","DOI":"10.4046\/trd.2016.79.1.5","article-title":"The role of innate and adaptive immune cells in the immunopathogenesis of chronic obstructive pulmonary disease","volume":"79","author":"Nurwidya","year":"2016","journal-title":"Tuberc. Respir. Dis (Seoul)."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0145","first-page":"5","article-title":"India needs a national COPD prevention and control programme","volume":"60","author":"Salvi","year":"2012","journal-title":"J. Assoc. Physicians India"},{"key":"10.1016\/j.toxlet.2019.08.020_bib0150","article-title":"In vitro measurements of tracheal constriction using mice","volume":"3703","author":"Semenov","year":"2012","journal-title":"J. Vis. Exp."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0155","doi-asserted-by":"crossref","first-page":"1931","DOI":"10.1164\/ajrccm.151.6.7767542","article-title":"Increased airway responsiveness to inhaled methacholine in a rat model of chronic bronchitis","volume":"151","author":"Shore","year":"1995","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0160","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1158\/1940-6207.CAPR-08-0174","article-title":"The field of tissue injury in the lung and airway","volume":"1","author":"Steiling","year":"2008","journal-title":"Cancer Prev. Res. Phila. (Phila)"},{"key":"10.1016\/j.toxlet.2019.08.020_bib0165","doi-asserted-by":"crossref","first-page":"14971","DOI":"10.1038\/srep14971","article-title":"Metabolic changes of H2S in smokers and patients of COPD which might involve in inflammation, oxidative stress and steroid sensitivity","volume":"5","author":"Sun","year":"2015","journal-title":"Sci. Rep."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0170","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.puhe.2018.02.016","article-title":"Chronic obstructive pulmonary disease deaths, disability-adjusted life years, and risk factors in Hubei province of mid-China, 1990-2015: the global burden of disease study 2015","volume":"161","author":"Tang","year":"2018","journal-title":"Public Health"},{"key":"10.1016\/j.toxlet.2019.08.020_bib0175","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.1073\/pnas.032668799","article-title":"Engineering gene expression and protein synthesis by modulation of nuclear shape","volume":"99","author":"Thomas","year":"2002","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0180","doi-asserted-by":"crossref","first-page":"52","DOI":"10.2144\/btn-2017-0100","article-title":"A triple co-culture method to investigate the effect of macrophages and fibroblasts on myoblast proliferation and migration","volume":"64","author":"Venter","year":"2018","journal-title":"Biotechniques."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0185","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.1016\/j.biocel.2003.11.015","article-title":"Modifications of chromatin structure and gene expression following induced alterations of cellular shape","volume":"36","author":"Vergani","year":"2004","journal-title":"Int. J. Biochem. Cell Biol."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0190","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3389\/fimmu.2014.00435","article-title":"Role of alveolar macrophages in chronic obstructive pulmonary disease","volume":"5","author":"Vlahos","year":"2014","journal-title":"Front. Immunol."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0195","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.pupt.2005.02.006","article-title":"Modelling COPD in mice","volume":"19","author":"Vlahos","year":"2006","journal-title":"Pulm. Pharmacol. Ther."},{"key":"10.1016\/j.toxlet.2019.08.020_bib0200","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s12192-016-0745-x","article-title":"Calreticulin attenuated microwave radiation-induced human microvascular endothelial cell injury through promoting actin acetylation and polymerization","volume":"22","author":"Xu","year":"2017","journal-title":"Cell Stress Chaperones"}],"container-title":["Toxicology Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378427419302346?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0378427419302346?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T06:15:32Z","timestamp":1573107332000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0378427419302346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":40,"alternative-id":["S0378427419302346"],"URL":"https:\/\/doi.org\/10.1016\/j.toxlet.2019.08.020","relation":{},"ISSN":["0378-4274"],"issn-type":[{"value":"0378-4274","type":"print"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Establishment of rapid risk assessment model for cigarette smoke extract exposure in chronic obstructive pulmonary disease","name":"articletitle","label":"Article Title"},{"value":"Toxicology Letters","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.toxlet.2019.08.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier B.V. All rights reserved.","name":"copyright","label":"Copyright"}]}}