{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,22]],"date-time":"2026-01-22T17:00:59Z","timestamp":1769101259709,"version":"3.49.0"},"reference-count":37,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100005632","name":"Narodowe Centrum Bada\u0144 i Rozwoju","doi-asserted-by":"publisher","award":["STRATERMED2\/269807\/14\/NCBR\/2015"],"award-info":[{"award-number":["STRATERMED2\/269807\/14\/NCBR\/2015"]}],"id":[{"id":"10.13039\/501100005632","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Transplantation Proceedings"],"published-print":{"date-parts":[[2020,9]]},"DOI":"10.1016\/j.transproceed.2020.02.115","type":"journal-article","created":{"date-parts":[[2020,4,24]],"date-time":"2020-04-24T21:25:34Z","timestamp":1587763534000},"page":"2204-2207","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":12,"title":["New Treatment of Wound Healing With Allogenic Acellular Human Skin\u00a0Graft: Preclinical Assessment and In\u00a0Vitro Study"],"prefix":"10.1016","volume":"52","author":[{"given":"M.","family":"Nita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Pliszczy\u0144ski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Kowalewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Wo\u017aniak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Eljaszewicz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Moniuszko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Kami\u0144ski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"\u015aladowski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Zimek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Majewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kosieradzki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Fiedor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.transproceed.2020.02.115_bib1","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/wrr.12387","article-title":"Epidemiology of chronic wounds in Germany: analysis of statutory health insurance data","volume":"24","author":"Heyer","year":"2016","journal-title":"Wound Repair Regen"},{"key":"10.1016\/j.transproceed.2020.02.115_bib2","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1111\/j.1524-475X.2010.00666.x","article-title":"Epidemiological of chronic cutaneous wounds in China","volume":"19","author":"Jiang","year":"2011","journal-title":"Wound Repair Regen"},{"key":"10.1016\/j.transproceed.2020.02.115_bib3","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1093\/qjmed\/hch075","article-title":"Prevalence of leg ulceration in a London population","volume":"97","author":"Moffatt","year":"2004","journal-title":"QJM"},{"key":"10.1016\/j.transproceed.2020.02.115_bib4","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1097\/01.ASW.0000363550.82058.7f","article-title":"The demographics of suspected deep tissue injury in the United States: an analysis of the International Pressure Ulcer Prevalence Survey 2006-2009","volume":"23","author":"VanGilder","year":"2010","journal-title":"Adv Skin Wound Care"},{"key":"10.1016\/j.transproceed.2020.02.115_bib5","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1111\/dth.12052","article-title":"Updating on understanding and managing chronic wound","volume":"26","author":"Park","year":"2013","journal-title":"Dermatol Ther"},{"key":"10.1016\/j.transproceed.2020.02.115_bib6","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1177\/1534734617696730","article-title":"A\u00a0clinicoepidemiological profile of chronic wounds in wound healing department in Shanghai","volume":"16","author":"Sun","year":"2017","journal-title":"Int J Low Extrem Wounds"},{"key":"10.1016\/j.transproceed.2020.02.115_bib7","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/wrr.12370","article-title":"Analysis of the chronic wound microbiota of 2,963 patients by 16S rDNA pyrosequencing","volume":"24","author":"Wolcott","year":"2016","journal-title":"Wound Repair Regen"},{"key":"10.1016\/j.transproceed.2020.02.115_bib8","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1111\/jgs.13332","article-title":"Chronic wound repair and healing in older adults: current status and future research","volume":"63","author":"Gould","year":"2015","journal-title":"J\u00a0Am Geriatr Soc"},{"key":"10.1016\/j.transproceed.2020.02.115_bib9","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1007\/s00404-015-3709-y","article-title":"Growth factors, silver dressings and negative pressure wound therapy in the management of hard-to-heal postoperative wounds in obstetrics and gynecology: a review","volume":"292","author":"Stanirowski","year":"2015","journal-title":"Arch Gynecol Obstet"},{"key":"10.1016\/j.transproceed.2020.02.115_bib10","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1111\/j.1742-481X.2007.00406.x","article-title":"Topical antimicrobial therapy of chronic wounds healing by secondary intention using iodine products","volume":"5","author":"Leaper","year":"2008","journal-title":"Int Wound J"},{"key":"10.1016\/j.transproceed.2020.02.115_bib11","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1111\/jgs.13475","article-title":"Under pressure: financial effect of the hospital-acquired conditions initiative\u2013a statewide analysis of pressure ulcer development and payment","volume":"63","author":"Meddings","year":"2015","journal-title":"J\u00a0Am Geriatr Soc"},{"key":"10.1016\/j.transproceed.2020.02.115_bib12","doi-asserted-by":"crossref","first-page":"38S","DOI":"10.1016\/S0002-9610(03)00303-9","article-title":"A\u00a0specialized wound-healing center concept: importance of a multidisciplinary department structure and surgical treatment facilities in the treatment of chronic wounds","volume":"187","author":"Gottrup","year":"2004","journal-title":"Am J Surg"},{"key":"10.1016\/j.transproceed.2020.02.115_bib13","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/0969-806X(93)90313-J","article-title":"EB industrial facility for radiation sterilization of medical devices","volume":"42","author":"Zimek","year":"1993","journal-title":"Radiat Phys Chem"},{"key":"10.1016\/j.transproceed.2020.02.115_bib14","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1016\/0969-806X(95)00361-Z","article-title":"Polystyrene calorimeter for electron beam dose measurements","volume":"46","author":"Miller","year":"1993","journal-title":"Radiat Phys Chem"},{"key":"10.1016\/j.transproceed.2020.02.115_bib15","unstructured":"National Physical Laboratory, Teddington, Middlesex, UK, www.npl.co.uk. [accessed 05.04.20]."},{"key":"10.1016\/j.transproceed.2020.02.115_bib16","first-page":"205","article-title":"Does leg ulcer treatment improve patient\u2019s quality of life?","volume":"13","author":"Charles","year":"2003","journal-title":"J\u00a0Wound Care"},{"key":"10.1016\/j.transproceed.2020.02.115_bib17","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/bjd.15572","article-title":"Wound healing in epidermolysis bullosa","volume":"177","author":"Bruckner-Tuderman","year":"2017","journal-title":"Br J Dermatol"},{"key":"10.1016\/j.transproceed.2020.02.115_bib18","unstructured":"Denyer J, Pillay E. Best practice guidelines for skin and wound care in epidermolysis bullosa, DEBRA: International Consensus. http:\/\/www.woundsinternational.com\/media\/issues\/623\/files\/content_10609.pdf; 2012 [accessed 10.08.17]."},{"key":"10.1016\/j.transproceed.2020.02.115_bib19","doi-asserted-by":"crossref","first-page":"5068969","DOI":"10.1155\/2017\/5068969","article-title":"Betulin-based oleogel to improve wound healing in dystrophic epidermolysis bullosa: a prospective controlled proof-of-concept study","volume":"2017","author":"Schwieger-Briel","year":"2017","journal-title":"Dermatol Res Pract"},{"key":"10.1016\/j.transproceed.2020.02.115_bib20","doi-asserted-by":"crossref","first-page":"e212","DOI":"10.1097\/BCR.0000000000000357","article-title":"Local burn injury promotes defects in the epidermal lipid and antimicrobial peptide barriers in human autograft skin and burn margin: implications for burn wound healing and graft survival","volume":"38","author":"Plichta","year":"2017","journal-title":"J\u00a0Burn Care Res"},{"key":"10.1016\/j.transproceed.2020.02.115_bib21","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1097\/BCR.0b013e318235836e","article-title":"Immunoprotective role of indoleamine 2,3-dioxygenase in engraftment of allogenic skin substitute in wound healing","volume":"33","author":"Bahar","year":"2012","journal-title":"J\u00a0Burn Care Res"},{"key":"10.1016\/j.transproceed.2020.02.115_bib22","first-page":"9","article-title":"The use of a noncultured autologous cell suspension to repair chronic ulcers","author":"De Angelis","year":"2015","journal-title":"Int Wound J"},{"key":"10.1016\/j.transproceed.2020.02.115_bib23","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/wrr.12029","article-title":"The role of skin substitutes in the management of chronic cutaneous wounds","volume":"21","author":"Greaves","year":"2013","journal-title":"Wound Repair Regen"},{"key":"10.1016\/j.transproceed.2020.02.115_bib24","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1177\/1534734605282999","article-title":"A\u00a0prospective multicenter study of the efficacy and tolerability of cryopreserved allogenic human keratinocytes to treat venous leg ulcers","volume":"4","author":"Beele","year":"2005","journal-title":"Int J Low Extrem Wounds"},{"key":"10.1016\/j.transproceed.2020.02.115_bib25","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.burns.2011.03.001","article-title":"Characterisation of the cell suspension harvested from the dermal epidermal junction using a ReCellkit","volume":"38","author":"Wood","year":"2012","journal-title":"Burns"},{"key":"10.1016\/j.transproceed.2020.02.115_bib26","doi-asserted-by":"crossref","first-page":"271","DOI":"10.5301\/IJAO.2011.6508","article-title":"Method for autologous single skin cell isolation for regenerative cell spray transplantation with non-cultured cells","volume":"34","author":"Gerlach","year":"2011","journal-title":"Int J Artif Organs"},{"key":"10.1016\/j.transproceed.2020.02.115_bib27","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1097\/01.ASW.0000363551.82058.36","article-title":"Tissue regeneration in loss of substance on the lower limbs through use of platelet rich plasma, stem cells from adipose tissue, and hyaluronic acid","volume":"23","author":"Cervelli","year":"2010","journal-title":"Adv Skin Wound Care"},{"key":"10.1016\/j.transproceed.2020.02.115_bib28","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.gde.2006.08.006","article-title":"Epidermal stem cells: an update","volume":"16","author":"Watt","year":"2006","journal-title":"Curr Opin Genet Dev"},{"key":"10.1016\/j.transproceed.2020.02.115_bib29","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1038\/ng1744","article-title":"Morphogenesis in skin is governed by discrete sets of differentially expressed microRNAs","volume":"38","author":"Yi","year":"2006","journal-title":"Nat Genet"},{"key":"10.1016\/j.transproceed.2020.02.115_bib30","doi-asserted-by":"crossref","first-page":"3498","DOI":"10.1172\/JCI68127","article-title":"Collagen VII plays a dual role in wound healing","volume":"123","author":"Nystrom","year":"2013","journal-title":"J\u00a0Clin Invest"},{"key":"10.1016\/j.transproceed.2020.02.115_bib31","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1016\/j.burns.2006.10.388","article-title":"The use of a non- cultured autologous cell suspension and Integra dermal regeneration template to repair full-thickness skin wounds in a porcine model: a one-step process","volume":"33","author":"Wood","year":"2007","journal-title":"Burns"},{"key":"10.1016\/j.transproceed.2020.02.115_bib32","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0007-1226(52)80004-9","article-title":"Transplantation studies on sheets of pure epidermal epithelium and on epidermal cell suspensions","volume":"5","author":"Billingham","year":"1952","journal-title":"Br J Plast Surg"},{"key":"10.1016\/j.transproceed.2020.02.115_bib33","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0092-8674(75)80001-8","article-title":"Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells","volume":"6","author":"Rheinwald","year":"1975","journal-title":"Cell"},{"key":"10.1016\/j.transproceed.2020.02.115_bib34","doi-asserted-by":"crossref","first-page":"1018","DOI":"10.1038\/sj.jid.5700715","article-title":"Tissue engineering for cutaneous wounds","volume":"127","author":"Clark","year":"2007","journal-title":"J\u00a0Invest Dermatol"},{"key":"10.1016\/j.transproceed.2020.02.115_bib35","doi-asserted-by":"crossref","first-page":"5665","DOI":"10.1073\/pnas.76.11.5665","article-title":"Growth of cultured human epidermal cells into multiple epithelia suitable for grafting","volume":"76","author":"Green","year":"1979","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.transproceed.2020.02.115_bib36","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1038\/265421a0","article-title":"Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes","volume":"265","author":"Rheinwald","year":"1977","journal-title":"Nature"},{"key":"10.1016\/j.transproceed.2020.02.115_bib37","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1111\/j.1524-475X.2007.00231.x","article-title":"Treatment of recalcitrant venous leg ulcers with autologous keratinocytes in fibrin sealant: a multinational randomized controlled clinical trial","volume":"15","author":"Vanscheidt","year":"2007","journal-title":"Wound Repair Regen"}],"container-title":["Transplantation Proceedings"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041134520302086?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0041134520302086?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T21:36:15Z","timestamp":1758576975000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0041134520302086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":37,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["S0041134520302086"],"URL":"https:\/\/doi.org\/10.1016\/j.transproceed.2020.02.115","relation":{},"ISSN":["0041-1345"],"issn-type":[{"value":"0041-1345","type":"print"}],"subject":[],"published":{"date-parts":[[2020,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"New Treatment of Wound Healing With Allogenic Acellular Human Skin\u00a0Graft: Preclinical Assessment and In\u00a0Vitro Study","name":"articletitle","label":"Article Title"},{"value":"Transplantation Proceedings","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.transproceed.2020.02.115","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Published by Elsevier Inc.","name":"copyright","label":"Copyright"}]}}