{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:47:16Z","timestamp":1771030036387,"version":"3.50.1"},"reference-count":112,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Transplant Immunology"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.trim.2025.102342","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:32:07Z","timestamp":1765326727000},"page":"102342","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":1,"special_numbering":"C","title":["mRNA vaccine for human cytomegalovirus in immunocompromised hosts: An immunoinformatics approach"],"prefix":"10.1016","volume":"94","author":[{"given":"Muhammad Zeeshan","family":"Ahmed","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sana","family":"Dilbar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Syeda Masooma Zahra","family":"Bukhari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zareena","family":"Ali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binyamin","family":"Wattoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sayra","family":"Tariq","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeeshan","family":"Mutahir","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noreen","family":"Samad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.trim.2025.102342_bb0005","doi-asserted-by":"crossref","DOI":"10.1002\/rmv.2034","article-title":"Estimation of the worldwide seroprevalence of cytomegalovirus: a systematic review and meta-analysis","volume":"29","author":"Zuhair","year":"2019","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.trim.2025.102342_bb0010","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1002\/rmv.655","article-title":"Review of cytomegalovirus seroprevalence and demographic characteristics associated with infection","volume":"20","author":"Cannon","year":"2010","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.trim.2025.102342_bb0015","series-title":"Cytomegalovirus (CMV) and Congenital CMV Infection","author":"CDC","year":"2025"},{"key":"10.1016\/j.trim.2025.102342_bb0020","first-page":"827","article-title":"Human cytomegalovirus: a neglected public health area of significant relevance to women, the foetus and new born. Time for action!","volume":"67","author":"Siddiqui","year":"2017","journal-title":"J. Pak. Med. Assoc."},{"key":"10.1016\/j.trim.2025.102342_bb0025","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1159\/000322141","article-title":"Human cytomegalovirus (HCMV)-revised","volume":"37","author":"Seitz","year":"2010","journal-title":"Transfus. Med. Hemother."},{"key":"10.1016\/j.trim.2025.102342_bb0030","first-page":"49","article-title":"Human cytomegalovirus (HCMV) genetic diversity, drug resistance testing and prevalence of the resistance mutations: a literature review, trop med","volume":"9","author":"Grgic","year":"2024","journal-title":"Infect. Dis. Ther."},{"key":"10.1016\/j.trim.2025.102342_bb0035","article-title":"Challenges, recent advances and perspectives in the treatment of human cytomegalovirus infections","volume":"7","author":"Chen","year":"2022","journal-title":"Trop. Med. Infect. Dis."},{"key":"10.1016\/j.trim.2025.102342_bb0040","doi-asserted-by":"crossref","first-page":"515","DOI":"10.6061\/clinics\/2015(07)09","article-title":"Cytomegalovirus infection in transplant recipients","volume":"70","author":"Azevedo","year":"2015","journal-title":"Clinics"},{"key":"10.1016\/j.trim.2025.102342_bb0050","first-page":"46","article-title":"The prevalence of human cytomegalovirus viremia among HIV-1 infected individuals undergoing antiretroviral therapy","volume":"11","author":"Bleiblo","year":"2023","journal-title":"J. Biosci. Med."},{"key":"10.1016\/j.trim.2025.102342_bb0055","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/rmv.2117","article-title":"Impact of breast milk-acquired cytomegalovirus infection in premature infants: pathogenesis, prevention, and clinical consequences?","volume":"30","author":"Osterholm","year":"2020","journal-title":"Rev. Med. Virol."},{"key":"10.1016\/j.trim.2025.102342_bb0065","doi-asserted-by":"crossref","first-page":"S14","DOI":"10.1093\/jpids\/piad059","article-title":"Current and emerging antiviral agents in the prevention and treatment of cytomegalovirus in pediatric transplant recipients","volume":"13","author":"Deray","year":"2024","journal-title":"J. Pediatric Infect. Dis. Soc."},{"key":"10.1016\/j.trim.2025.102342_bb0070","doi-asserted-by":"crossref","first-page":"1693","DOI":"10.1016\/j.chembiol.2021.06.001","article-title":"NUDT15-mediated hydrolysis limits the efficacy of anti-HCMV drug ganciclovir","volume":"28","author":"Zhang","year":"2021","journal-title":"Cell Chem. Biol."},{"key":"10.1016\/j.trim.2025.102342_bb0075","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1080\/21645515.2017.1391433","article-title":"Human cytomegalovirus vaccine development: immune responses to look into vaccine strategy","volume":"14","author":"Xia","year":"2018","journal-title":"Hum. Vaccin. Immunother."},{"key":"10.1016\/j.trim.2025.102342_bb0080","doi-asserted-by":"crossref","first-page":"7437","DOI":"10.1016\/j.vaccine.2018.02.089","article-title":"Vaccination against the human cytomegalovirus","volume":"37","author":"Plotkin","year":"2019","journal-title":"Vaccine"},{"key":"10.1016\/j.trim.2025.102342_bb0085","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1007\/s40265-016-0653-5","article-title":"Cytomegalovirus vaccines: current status and future prospects","volume":"76","author":"Anderholm","year":"2016","journal-title":"Drugs"},{"key":"10.1016\/j.trim.2025.102342_bb0090","series-title":"The Long History of mRNA Vaccines","author":"Beyrer","year":"2021"},{"key":"10.1016\/j.trim.2025.102342_bb0095","article-title":"The safety and effectiveness of mRNA vaccines against SARS-CoV-2","volume":"15","author":"Jamous","year":"2023","journal-title":"Cureus"},{"key":"10.1016\/j.trim.2025.102342_bb0100","doi-asserted-by":"crossref","first-page":"2427","DOI":"10.1056\/NEJMoa2028436","article-title":"Safety and immunogenicity of SARS-CoV-2 mRNA-1273 vaccine in older adults","volume":"383","author":"Anderson","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"10.1016\/j.trim.2025.102342_bb0105","doi-asserted-by":"crossref","first-page":"1972","DOI":"10.3390\/pharmaceutics15071972","article-title":"Recent advancement in mRNA vaccine development and applications","volume":"15","author":"Al Fayez","year":"2023","journal-title":"Pharmaceutics"},{"key":"10.1016\/j.trim.2025.102342_bb0110","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.01603-23","article-title":"Characterization of humoral and cellular immunologic responses to an mRNA-based human cytomegalovirus vaccine from a phase 1 trial of healthy adults","volume":"98","author":"Wu","year":"2024","journal-title":"J. Virol."},{"key":"10.1016\/j.trim.2025.102342_bb0115","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1186\/s12859-019-3052-6","article-title":"Computational assembly of a human cytomegalovirus vaccine upon experimental epitope legacy","volume":"20","author":"Quinzo","year":"2019","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.trim.2025.102342_bb0120","series-title":"New Vaccine Study for Human Cytomegalovirus","author":"Abene","year":"2024"},{"key":"10.1016\/j.trim.2025.102342_bb0125","series-title":"Moderna Scraps mRNA Vaccine in Congenital CMV on Phase III Failure","author":"Beaney","year":"2025"},{"key":"10.1016\/j.trim.2025.102342_bb0130","year":"2025","journal-title":"Reuters"},{"key":"10.1016\/j.trim.2025.102342_bb0140","doi-asserted-by":"crossref","first-page":"203","DOI":"10.3390\/vaccines11020203","article-title":"Targeted protein-specific multi-epitope-based vaccine designing against human cytomegalovirus by using Immunoinformatics approaches","volume":"11","author":"Bakkari","year":"2023","journal-title":"Vaccines"},{"key":"10.1016\/j.trim.2025.102342_bb0145","doi-asserted-by":"crossref","DOI":"10.1016\/j.ejps.2020.105279","article-title":"Immuno-informatics approach to design a multi-epitope vaccine to combat cytomegalovirus infection","volume":"147","author":"Chauhan","year":"2020","journal-title":"Eur. J. Pharm. Sci."},{"key":"10.1016\/j.trim.2025.102342_bb0150","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1080\/07391102.2021.2014969","article-title":"Computational designing of a novel subunit vaccine for human cytomegalovirus by employing the immunoinformatics framework","volume":"41","author":"Moin","year":"2023","journal-title":"J. Biomol. Struct. Dyn."},{"key":"10.1016\/j.trim.2025.102342_bb0155","doi-asserted-by":"crossref","DOI":"10.1016\/j.biologicals.2024.101782","article-title":"Designing multi-epitope vaccine against human cytomegalovirus integrating pan-genome and reverse vaccinology pipelines","volume":"87","author":"Biswas","year":"2024","journal-title":"Biologicals"},{"key":"10.1016\/j.trim.2025.102342_bb0160","doi-asserted-by":"crossref","first-page":"435","DOI":"10.3390\/vaccines13050435","article-title":"Developing a vaccine against human cytomegalovirus: identifying and targeting HCMV\u2019s immunological achilles\u2019 heel","volume":"13","author":"Lankina","year":"2025","journal-title":"Vaccines"},{"key":"10.1016\/j.trim.2025.102342_bb0165","doi-asserted-by":"crossref","first-page":"551","DOI":"10.3390\/vaccines9060551","article-title":"Development of a vaccine against human cytomegalovirus: advances, barriers, and implications for the clinical practice","volume":"9","author":"Scarpini","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.trim.2025.102342_bb0170","doi-asserted-by":"crossref","first-page":"e668","DOI":"10.1093\/infdis\/jiae114","article-title":"Safety and immunogenicity of a messenger RNA\u2013based cytomegalovirus vaccine in healthy adults: results from a phase 1 randomized clinical trial","volume":"230","author":"Fierro","year":"2024","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.trim.2025.102342_bb0175","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1080\/14760584.2024.2354251","article-title":"Research progress on the quality control of mRNA vaccines","volume":"23","author":"Hu","year":"2024","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.trim.2025.102342_bb0180","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1016\/j.vaccine.2018.01.029","article-title":"Multi-antigenic human cytomegalovirus mRNA vaccines that elicit potent humoral and cell-mediated immunity","volume":"36","author":"John","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.trim.2025.102342_bb0185","doi-asserted-by":"crossref","DOI":"10.1080\/21645515.2024.2311974","article-title":"Multi-epitope mRNA vaccine candidate to combat HMPV virus: Comment","volume":"20","author":"Daungsupawong","year":"2024","journal-title":"Hum. Vaccin. Immunother."},{"key":"10.1016\/j.trim.2025.102342_bb0190","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2025.1658220","article-title":"Optimized multi-epitope neoantigen human cytomegalovirus vaccine based on adenovirus vectors elicits potent antiviral immunity","volume":"16","author":"Jiang","year":"2025","journal-title":"Front. Immunol."},{"key":"10.1016\/j.trim.2025.102342_bb0195","doi-asserted-by":"crossref","DOI":"10.1080\/21645515.2025.2450045","article-title":"The mRNA-1647 vaccine: a promising step toward the prevention of cytomegalovirus infection (CMV)","volume":"21","author":"Akingbola","year":"2025","journal-title":"Hum. Vaccin. Immunother."},{"key":"10.1016\/j.trim.2025.102342_bb0200","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1016\/j.virol.2015.03.043","article-title":"Viral membrane fusion","volume":"479\u2013480","author":"Harrison","year":"2015","journal-title":"Virology"},{"key":"10.1016\/j.trim.2025.102342_bb0205","doi-asserted-by":"crossref","first-page":"E4997","DOI":"10.1073\/pnas.1316517110","article-title":"Pentameric complex of viral glycoprotein H is the primary target for potent neutralization by a human cytomegalovirus vaccine","volume":"110","author":"Freed","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.trim.2025.102342_bb0210","doi-asserted-by":"crossref","DOI":"10.1128\/jvi.02178-24","article-title":"A human cytomegalovirus prefusion-like glycoprotein B subunit vaccine elicits humoral immunity similar to that of postfusion gB in mice","volume":"99","author":"Karthigeyan","year":"2025","journal-title":"J. Virol."},{"key":"10.1016\/j.trim.2025.102342_bb0215","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1093\/infdis\/jiad593","article-title":"Human cytomegalovirus mRNA-1647 vaccine candidate elicits potent and broad neutralization and higher antibody-dependent cellular cytotoxicity responses than the gB\/MF59 vaccine","volume":"230","author":"Hu","year":"2024","journal-title":"J. Infect. Dis."},{"issue":"1","key":"10.1016\/j.trim.2025.102342_bb0220","doi-asserted-by":"crossref","DOI":"10.1038\/s41541-024-00821-3","article-title":"Multivalent cytomegalovirus glycoprotein B nucleoside modified mRNA vaccines did not demonstrate a greater antibody breadth","volume":"9","author":"Wang","year":"2024","journal-title":"npj Vaccines."},{"key":"10.1016\/j.trim.2025.102342_bb0225","doi-asserted-by":"crossref","DOI":"10.1128\/JVI.01442-19","article-title":"Inclusion of the viral pentamer complex in a vaccine design greatly improves protection against congenital cytomegalovirus in the Guinea pig model","volume":"93","author":"Choi","year":"2019","journal-title":"J. Virol."},{"issue":"12","key":"10.1016\/j.trim.2025.102342_bb0230","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1038\/s41579-021-00582-z","article-title":"Pathogenesis of human cytomegalovirus in the immunocompromised host","volume":"19","author":"Griffiths","year":"2021","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.trim.2025.102342_bb0235","first-page":"22","article-title":"Bioinformatic approach for identifying parasite and fungal candidate subunit vaccines","volume":"3","author":"Doytchinova","year":"2010","journal-title":"Open Vaccine J."},{"key":"10.1016\/j.trim.2025.102342_bb0240","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1016\/j.vaccine.2006.09.032","article-title":"Identifying candidate subunit vaccines using an alignment-independent method based on principal amino acid properties","volume":"25","author":"Doytchinova","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.trim.2025.102342_bb0245","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2105-8-4","article-title":"VaxiJen: a server for prediction of protective antigens, tumour antigens and subunit vaccines","volume":"8","author":"Doytchinova","year":"2007","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.trim.2025.102342_bb0250","series-title":"The Proteomics Protocols Handbook","first-page":"571","article-title":"Protein identification and analysis tools on the ExPASy server","author":"Gasteiger","year":"2005"},{"key":"10.1016\/j.trim.2025.102342_bb0255","series-title":"The Proteomics Protocols Handbook","author":"Walker","year":"2005"},{"key":"10.1016\/j.trim.2025.102342_bb0260","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2105-7-153","article-title":"Predicting population coverage of T-cell epitope-based diagnostics and vaccines","volume":"7","author":"Bui","year":"2006","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.trim.2025.102342_bb0265","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10989-022-10438-6","article-title":"Exploratory algorithm of a multi-epitope-based subunit vaccine candidate against Cryptosporidium hominis: reverse vaccinology-based immunoinformatic approach","volume":"28","author":"Hasan","year":"2022","journal-title":"Int. J. Pept. Res. Ther."},{"key":"10.1016\/j.trim.2025.102342_bb0270","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2025.1480025","article-title":"Immunoinformatic-driven design and evaluation of multi-epitope mRNA vaccine targeting HIV-1 gp120","volume":"16","author":"Ahmed","year":"2025","journal-title":"Front. Immunol."},{"key":"10.1016\/j.trim.2025.102342_bb0275","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.virol.2016.04.027","article-title":"Novel 6xHis tagged foot-and-mouth disease virus vaccine bound to nanolipoprotein adjuvant via metal ions provides antigenic distinction and effective protective immunity","volume":"495","author":"Rai","year":"2016","journal-title":"Virology"},{"key":"10.1016\/j.trim.2025.102342_bb0280","first-page":"1","article-title":"In silico design and evaluation of a novel mRNA vaccine against BK virus: a reverse vaccinology approach","volume":"1","author":"Mohammadi","year":"2022","journal-title":"Immunol. Res."},{"key":"10.1016\/j.trim.2025.102342_bb0285","doi-asserted-by":"crossref","first-page":"4645","DOI":"10.1038\/s41598-022-08506-4","article-title":"Novel in silico mRNA vaccine design exploiting proteins of M. tuberculosis that modulates host immune responses by inducing epigenetic modifications","volume":"12","author":"Al Tbeishat","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.trim.2025.102342_bb0290","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/j.ijbiomac.2020.06.213","article-title":"Designing a novel mRNA vaccine against SARS-CoV-2: an immunoinformatics approach","volume":"162","author":"Ahammad","year":"2020","journal-title":"Int. J. Biol. Macromol."},{"key":"10.1016\/j.trim.2025.102342_bb0295","doi-asserted-by":"crossref","first-page":"W526","DOI":"10.1093\/nar\/gki376","article-title":"JCat: a novel tool to adapt codon usage of a target gene to its potential expression host","volume":"33","author":"Grote","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.trim.2025.102342_bb0300","doi-asserted-by":"crossref","first-page":"7287","DOI":"10.1073\/pnas.0401799101","article-title":"Incorporating chemical modification constraints into a dynamic programming algorithm for prediction of RNA secondary structure","volume":"101","author":"Mathews","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"10.1016\/j.trim.2025.102342_bb0305","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1748-7188-6-26","article-title":"ViennaRNA package 2.0","volume":"6","author":"Lorenz","year":"2011","journal-title":"Algorithms Mol. Biol."},{"key":"10.1016\/j.trim.2025.102342_bb0310","doi-asserted-by":"crossref","first-page":"W70","DOI":"10.1093\/nar\/gkn188","article-title":"The Vienna RNA websuite","volume":"36","author":"Gruber","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.trim.2025.102342_bb0315","doi-asserted-by":"crossref","first-page":"2278","DOI":"10.1007\/s00894-014-2278-5","article-title":"AllerTOP v.2 - a server for in silico prediction of allergens","volume":"20","author":"Dimitrov","year":"2014","journal-title":"J. Mol. Model."},{"key":"10.1016\/j.trim.2025.102342_bb0320","doi-asserted-by":"crossref","first-page":"681","DOI":"10.1093\/bioinformatics\/11.6.681","article-title":"Sopma: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments","volume":"11","author":"Geourjon","year":"1995","journal-title":"Bioinformatics"},{"key":"10.1016\/j.trim.2025.102342_bb0325","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1006\/jmbi.1999.3091","article-title":"Protein secondary structure prediction based on position-specific scoring matrices","volume":"292","author":"Jones","year":"1999","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.trim.2025.102342_bb0330","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1093\/bioinformatics\/16.4.404","article-title":"The PSIPRED protein structure prediction server","volume":"16","author":"McGuffin","year":"2000","journal-title":"Bioinformatics"},{"key":"10.1016\/j.trim.2025.102342_bb0335","doi-asserted-by":"crossref","first-page":"W349","DOI":"10.1093\/nar\/gkt381","article-title":"Scalable web services for the PSIPRED protein analysis workbench","volume":"41","author":"Buchan","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.trim.2025.102342_bb0340","doi-asserted-by":"crossref","first-page":"W294","DOI":"10.1093\/nar\/gks493","article-title":"GalaxyWEB server for protein structure prediction and refinement","volume":"40","author":"Ko","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.trim.2025.102342_bb0345","series-title":"International Tables of Crystallography, Volume F. Crystallography of Biological Macromolecules","first-page":"684","article-title":"PROCHECK: validation of protein-structure coordinates","author":"Laskowski","year":"2012"},{"key":"10.1016\/j.trim.2025.102342_bb0350","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1471-2105-9-514","article-title":"ElliPro: a new structure-based tool for the prediction of antibody epitopes","volume":"9","author":"Ponomarenko","year":"2008","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.trim.2025.102342_bb0355","doi-asserted-by":"crossref","DOI":"10.1016\/j.sjbs.2023.103661","article-title":"Harnessing immunoinformatics for developing a multiple-epitope peptide-based vaccination approach against SARS-CoV-2 spike protein","volume":"30","author":"Moustafa","year":"2023","journal-title":"Saudi J. Biol. Sci."},{"key":"10.1016\/j.trim.2025.102342_bb0360","article-title":"Reverse vaccinology approach towards the in-silico multiepitope vaccine development against SARS-CoV-2","volume":"10","author":"Jena","year":"2021","journal-title":"F1000Res"},{"issue":"1","key":"10.1016\/j.trim.2025.102342_bb0365","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12979-023-00383-3","article-title":"Toll-like receptor 4 (TLR4): new insight immune and aging","volume":"20","author":"Kim","year":"2023","journal-title":"Immun. Ageing"},{"key":"10.1016\/j.trim.2025.102342_bb0370","article-title":"Toll-like receptor signaling and its role in cell-mediated immunity","author":"Duan","year":"2022","journal-title":"Front. Media SA."},{"key":"10.1016\/j.trim.2025.102342_bb0375","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1038\/nprot.2016.169","article-title":"The ClusPro web server for protein-protein docking","volume":"12","author":"Kozakov","year":"2017","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.trim.2025.102342_bb0380","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1002\/prot.25219","article-title":"New additions to the ClusPro server motivated by CAPRI","volume":"85","author":"Vajda","year":"2017","journal-title":"Proteins"},{"key":"10.1016\/j.trim.2025.102342_bb0385","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1016\/j.str.2020.06.006","article-title":"Performance and its limits in rigid body protein-protein docking","volume":"28","author":"Desta","year":"2020","journal-title":"Structure"},{"key":"10.1016\/j.trim.2025.102342_bb0390","doi-asserted-by":"crossref","DOI":"10.1016\/j.imu.2021.100678","article-title":"Immunoinformatics aided-design of novel multi-epitope based peptide vaccine against Hendra henipavirus through proteome exploration","volume":"25","author":"Hossan","year":"2021","journal-title":"Inform. Med. Unlocked."},{"key":"10.1016\/j.trim.2025.102342_bb0395","doi-asserted-by":"crossref","first-page":"19087","DOI":"10.1038\/s41598-022-23550-w","article-title":"Bioinformatics approach for the construction of multiple epitope vaccine against omicron variant of SARS-CoV-2","volume":"12","author":"Zaib","year":"2022","journal-title":"Sci. Rep."},{"key":"10.1016\/j.trim.2025.102342_bb0400","doi-asserted-by":"crossref","DOI":"10.1093\/nar\/gku339","article-title":"IMODS: internal coordinates normal mode analysis server","volume":"42","author":"L\u00f3pez-Blanco","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.trim.2025.102342_bb0405","first-page":"1","article-title":"Molecular docking reveals that ToLCNDV-encoded AV2 and AV3 differentially interact with S. lycopersicum-encoded RDR1 to suppress host plant defense","volume":"XX","author":"Iqbal","year":"2025","journal-title":"Pol. J. Environ. Stud."},{"key":"10.1016\/j.trim.2025.102342_bb0410","doi-asserted-by":"crossref","first-page":"3433","DOI":"10.3389\/fimmu.2021.646972","article-title":"From infection to immunity: understanding the response to SARS-CoV2 through in-silico modeling","volume":"12","author":"Castiglione","year":"2021","journal-title":"Front. Immunol."},{"key":"10.1016\/j.trim.2025.102342_bb0415","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0009862","article-title":"Computational immunology meets bioinformatics: the use of prediction tools for molecular binding in the simulation of the immune system","volume":"5","author":"Rapin","year":"2010","journal-title":"PLoS One"},{"key":"10.1016\/j.trim.2025.102342_bb0420","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3390\/vaccines10081300","article-title":"Designing a novel peptide-based multi-epitope vaccine to evoke a robust immune response against pathogenic multidrug-resistant Providencia heimbachae","volume":"10","author":"Naveed","year":"2022","journal-title":"Vaccines"},{"key":"10.1016\/j.trim.2025.102342_bb0425","doi-asserted-by":"crossref","first-page":"5580","DOI":"10.1016\/j.vaccine.2018.07.056","article-title":"Novel trimeric human cytomegalovirus glycoprotein B elicits a high-titer neutralizing antibody response","volume":"36","author":"Cui","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.trim.2025.102342_bb0450","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1186\/s12879-025-11162-4","article-title":"Long-term prognosis following cytomegalovirus respiratory infection in immunocompromised and immunocompetent patients: a retrospective single-centre study","volume":"25","author":"Cao","year":"2025","journal-title":"BMC Infect. Dis."},{"key":"10.1016\/j.trim.2025.102342_bb0455","article-title":"A promising endeavor against human cytomegalovirus: predominant epitopes-based recombinant subunit vaccine RHEcIE1\/pp65\/pp150","volume":"16","author":"Li","year":"2025","journal-title":"Virulence"},{"key":"10.1016\/j.trim.2025.102342_bb0430","doi-asserted-by":"crossref","first-page":"30307","DOI":"10.1038\/s41598-024-76329-6","article-title":"Targeting serotonin receptors with phytochemicals \u2013 an in-silico study","volume":"14","author":"Elalouf","year":"2024","journal-title":"Sci Rep"},{"key":"10.1016\/j.trim.2025.102342_bb0435","doi-asserted-by":"crossref","DOI":"10.3389\/fviro.2025.1698340","article-title":"Human cytomegalovirus infection in the era of vaccine development: case series of immunocompromised patients","volume":"5","author":"Marascio","year":"2025","journal-title":"Front. Virol."},{"key":"10.1016\/j.trim.2025.102342_bb0440","doi-asserted-by":"crossref","first-page":"998","DOI":"10.3390\/pathogens14100998","article-title":"The immunoregulatory mechanisms of human cytomegalovirus from primary infection to reactivation","volume":"14","author":"Liu","year":"2025","journal-title":"Pathogens"},{"key":"10.1016\/j.trim.2025.102342_bb0445","doi-asserted-by":"crossref","DOI":"10.1016\/j.bjid.2025.104508","article-title":"Prevalence and investigation of cytomegalovirus (HCMV) in blood donors from the main blood establishment in Rio de Janeiro\/Brazil","volume":"29","author":"da Oliveira","year":"2025","journal-title":"Braz. J. Infect. Dis."},{"key":"10.1016\/j.trim.2025.102342_bb0460","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1186\/1471-2105-8-361","article-title":"Development of an epitope conservancy analysis tool to facilitate the design of epitope-based diagnostics and vaccines","volume":"8","author":"Bui","year":"2007","journal-title":"BMC Bioinform."},{"key":"10.1016\/j.trim.2025.102342_bb0465","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1038\/s41541-025-01258-y","article-title":"AI-driven epitope prediction: a systematic review, comparative analysis, and practical guide for vaccine development","volume":"10","author":"Villanueva-Flores","year":"2025","journal-title":"npj Vaccines."},{"key":"10.1016\/j.trim.2025.102342_bb0470","doi-asserted-by":"crossref","first-page":"1629","DOI":"10.3390\/vaccines11111629","article-title":"Re-evaluating human cytomegalovirus vaccine design: prediction of T cell epitopes","volume":"11","author":"Barry","year":"2023","journal-title":"Vaccines"},{"key":"10.1016\/j.trim.2025.102342_bb0475","doi-asserted-by":"crossref","DOI":"10.1002\/pro.4605","article-title":"IEDB-3D 2.0: structural data analysis within the immune epitope database","volume":"32","author":"Mendes","year":"2023","journal-title":"Protein Sci."},{"key":"10.1016\/j.trim.2025.102342_bb0480","doi-asserted-by":"crossref","first-page":"W502","DOI":"10.1093\/nar\/gkz452","article-title":"IEDB-AR: immune epitope database\u2014analysis resource in 2019","volume":"47","author":"Dhanda","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.trim.2025.102342_bb0485","doi-asserted-by":"crossref","DOI":"10.1038\/s41541-024-01046-0","article-title":"SPA14 liposomes combining saponin with fully synthetic TLR4 agonist provide adjuvanticity to hCMV vaccine candidate","volume":"9","author":"Luna","year":"2024","journal-title":"npj Vaccines."},{"key":"10.1016\/j.trim.2025.102342_bb0490","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2023.1249718","article-title":"TLR agonists as vaccine adjuvants in the prevention of viral infections: an overview","volume":"14","author":"Kayesh","year":"2023","journal-title":"Front. Microbiol."},{"key":"10.1016\/j.trim.2025.102342_bb0495","doi-asserted-by":"crossref","DOI":"10.1016\/j.jgeb.2025.100524","article-title":"An immunoinformatics approach in designing high-coverage mRNA multi-epitope vaccine against multivariant SARS-CoV-2","volume":"23","author":"Giri-Rachman","year":"2025","journal-title":"J. Genet. Eng. Biotechnol."},{"key":"10.1016\/j.trim.2025.102342_bb0500","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0044500","article-title":"Human cytomegalovirus induces TLR4 signaling components in monocytes altering TIRAP, TRAM and downstream interferon-beta and TNF-alpha expression","volume":"7","author":"Yew","year":"2012","journal-title":"PLoS One"},{"key":"10.1016\/j.trim.2025.102342_bb0510","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2023.1091941","article-title":"Immunoinformatics design of multivalent epitope vaccine against monkeypox virus and its variants using membrane-bound, enveloped, and extracellular proteins as targets","volume":"14","author":"Waqas","year":"2023","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.trim.2025.102342_bb0515","doi-asserted-by":"crossref","first-page":"20497","DOI":"10.1038\/s41598-020-77565-2","article-title":"Conserved epitopes with high HLA-I population coverage are targets of CD8+ T cells associated with high IFN-\u03b3 responses against all dengue virus serotypes","volume":"10","author":"Adikari","year":"2020","journal-title":"Sci. Rep."},{"key":"10.1016\/j.trim.2025.102342_bb0520","doi-asserted-by":"crossref","DOI":"10.1172\/JCI182317","article-title":"A vaccine against cytomegalovirus: how close are we?","volume":"135","author":"Permar","year":"2025","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.trim.2025.102342_bb0525","article-title":"Subtractive proteomic analysis to identify highly antigenic proteins and reverse vaccinology based novel vaccine development against Haemophillius parainfluenzae","volume":"14","author":"Ahmad","year":"2025","journal-title":"Sci. Rep."},{"key":"10.1016\/j.trim.2025.102342_bb0530","article-title":"Expression of mammalian proteins for diagnostics and therapeutics: a review","author":"JKK","year":"2022","journal-title":"Mol. Biol. Rep."},{"key":"10.1016\/j.trim.2025.102342_bb0535","doi-asserted-by":"crossref","first-page":"2859","DOI":"10.1016\/j.immuni.2021.10.017","article-title":"Vaccination in a humanized mouse model elicits highly protective PfCSP-targeting anti-malarial antibodies","volume":"54","author":"Kratochvil","year":"2021","journal-title":"Immunity"},{"key":"10.1016\/j.trim.2025.102342_bb0540","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.abf3685","article-title":"A bivalent Epstein-Barr virus vaccine induces neutralizing antibodies that block infection and confer immunity in humanized mice","volume":"14","author":"Wei","year":"2022","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.trim.2025.102342_bb0545","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2025.1658220","article-title":"Optimized multi-epitope neoantigen human cytomegalovirus vaccine based on adenovirus vectors elicits potent antiviral immunity","volume":"16","author":"Jiang","year":"2025","journal-title":"Front. Immunol."},{"issue":"1","key":"10.1016\/j.trim.2025.102342_bb0550","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1038\/s41392-024-02002-z","article-title":"Progress and prospects of mRNA-based drugs in pre-clinical and clinical applications","volume":"9","author":"Shi","year":"2024","journal-title":"Signal Transduct. Target. Ther."},{"key":"10.1016\/j.trim.2025.102342_bb0555","doi-asserted-by":"crossref","first-page":"S45","DOI":"10.1093\/infdis\/jiz428","article-title":"Immune correlates of protection against human cytomegalovirus acquisition, replication, and disease","volume":"221","author":"Nelson","year":"2020","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.trim.2025.102342_bb0560","doi-asserted-by":"crossref","DOI":"10.2142\/biophysico.bppb-v20.0022","article-title":"Quantitative analysis of protein dynamics using a deep learning technique combined with experimental cryo-EM density data and MD simulations","volume":"20","author":"Matsumoto","year":"2023","journal-title":"Biophys. Physicobiol."},{"key":"10.1016\/j.trim.2025.102342_bb0565","doi-asserted-by":"crossref","DOI":"10.1016\/j.jmb.2023.168059","article-title":"CryoRes: local resolution estimation of cryo-EM density maps by deep learning","volume":"435","author":"Dai","year":"2023","journal-title":"J. Mol. Biol."},{"key":"10.1016\/j.trim.2025.102342_bb0570","doi-asserted-by":"crossref","DOI":"10.15252\/embj.201899243","article-title":"One-step CRISPR \/Cas9 method for the rapid generation of human antibody heavy chain knock-in mice","volume":"37","author":"Lin","year":"2018","journal-title":"EMBO J."},{"key":"10.1016\/j.trim.2025.102342_bb0575","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1080\/14760584.2021.1874925","article-title":"Immunoinformatics-guided designing and in silico analysis of epitope-based polyvalent vaccines against multiple strains of human coronavirus (HCoV)","volume":"21","author":"Sarkar","year":"2022","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.trim.2025.102342_bb0580","doi-asserted-by":"crossref","DOI":"10.1186\/s12859-019-3052-6","article-title":"Computational assembly of a human cytomegalovirus vaccine upon experimental epitope legacy","volume":"20","author":"Quinzo","year":"2019","journal-title":"BMC Bioinform."}],"container-title":["Transplant Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0966327425001704?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0966327425001704?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T12:31:32Z","timestamp":1769257892000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0966327425001704"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":112,"alternative-id":["S0966327425001704"],"URL":"https:\/\/doi.org\/10.1016\/j.trim.2025.102342","relation":{},"ISSN":["0966-3274"],"issn-type":[{"value":"0966-3274","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"mRNA vaccine for human cytomegalovirus in immunocompromised hosts: An immunoinformatics approach","name":"articletitle","label":"Article Title"},{"value":"Transplant Immunology","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.trim.2025.102342","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"102342"}}