{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T07:52:45Z","timestamp":1782978765580,"version":"3.54.5"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transactions of the Royal Society of Tropical Medicine and Hygiene"],"published-print":{"date-parts":[[2004,6]]},"DOI":"10.1016\/j.trstmh.2003.10.012","type":"journal-article","created":{"date-parts":[[2004,4,14]],"date-time":"2004-04-14T09:27:58Z","timestamp":1081934878000},"page":"360-366","source":"Crossref","is-referenced-by-count":34,"title":["Evaluation of nested PCR for the diagnosis of scrub typhus among patients with acute pyrexia of unknown origin"],"prefix":"10.1093","volume":"98","author":[{"given":"Watcharee","family":"Saisongkorh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mongkol","family":"Chenchittikul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khachornsakdi","family":"Silpapojakul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"10.1016\/j.trstmh.2003.10.012_BIB1","doi-asserted-by":"crossref","first-page":"796","DOI":"10.4269\/ajtmh.1973.22.796","article-title":"Epidemiology of the acute fevers of unknown origin in South Vietnam: effect of laboratory support on clinical diagnosis","volume":"22","author":"Berman","year":"1973","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB2","doi-asserted-by":"crossref","first-page":"168","DOI":"10.4269\/ajtmh.1988.38.168","article-title":"Murine typhus among Khmers living in an evacuation site on the Thai\u2013Kampuchean border","volume":"38","author":"Brown","year":"1988","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB3","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/0035-9203(76)90127-9","article-title":"Scrub typhus: a common cause of illness in indigenous populations","volume":"70","author":"Brown","year":"1976","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB4","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.4269\/ajtmh.1983.32.1101","article-title":"Diagnostic criteria for scrub typhus: probability values for immunofluorescent antibody and Proteus OX-K agglutinin titres","volume":"23","author":"Brown","year":"1983","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB5","doi-asserted-by":"crossref","first-page":"311","DOI":"10.4269\/ajtmh.1984.33.311","article-title":"Febrile illness in Malaysia\u2014an analysis of 1629 hopitalized patients","volume":"33","author":"Brown","year":"1984","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB6","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1111\/imj.1955.4.2.91","article-title":"The investigations of fevers in Northern Queensland by mouse inoculation, with particular reference to scrub typhus","volume":"4","author":"Carley","year":"1955","journal-title":"Australasian Ann. Med."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB7","doi-asserted-by":"crossref","first-page":"2628","DOI":"10.1128\/JCM.29.11.2628-2630.1991","article-title":"Specific amplification of Rickettsia tsutsugamushi DNA from clinical specimens by polymerase chain reaction","volume":"29","author":"Furuya","year":"1991","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB8","doi-asserted-by":"crossref","first-page":"1637","DOI":"10.1128\/JCM.31.6.1637-1640.1993","article-title":"Serotype-specific amplification of Rickettsia tsutsugamushi DNA by nested polymerase chain reaction","volume":"31","author":"Furuya","year":"1993","journal-title":"J. Clin. Microbiol."},{"issue":"Suppl.","key":"10.1016\/j.trstmh.2003.10.012_BIB9","first-page":"229","volume":"53","author":"Gordon","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB10","doi-asserted-by":"crossref","first-page":"662","DOI":"10.7326\/0003-4819-68-3-662","article-title":"Potential medical problems in personnel returning from Vietnam","volume":"68","author":"Gilbert","year":"1968","journal-title":"Ann. Intern. Med."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB11","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1128\/JCM.32.6.1435-1439.1994","article-title":"Detection of Rickettsia tsugamushi in experimentally infected mice by PCR","volume":"32","author":"Kee","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB12","unstructured":"Leelarasmee, A., 1993. Acute fever of unknown origin, In: Aswapokee, N., Tiengrim, S., Likitnukul, S., Wipakul, A. (Eds.), Infectious Diseases Experiences in Thailand, first ed. Infectious Diseases Association of Thailand, Bangkok, pp. 1\u201312 (in Thai)."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB13","unstructured":"Ministry of Health, Thailand, 2000. 1999 Annual Epidemiology Surveillance Report. Division of Epidemiology, Ministry of Public Health, Nonthaburi, p. 284."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB14","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1111\/j.1348-0421.1992.tb02118.x","article-title":"Sensitivity of polymerase chain reaction assay for Rickettsia tsutsugamushi in patients\u2019 blood samples","volume":"36","author":"Murai","year":"1992","journal-title":"Microbiol. Immunol."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB15","doi-asserted-by":"crossref","first-page":"325","DOI":"10.4269\/ajtmh.1995.52.325","article-title":"Eradication of Rickettsia tsutsugamushi from patients\u2019 blood by chemotherapy, as assessed by the polymerase chain reaction","volume":"52","author":"Murai","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB16","doi-asserted-by":"crossref","first-page":"2858","DOI":"10.1128\/JCM.32.11.2858-2860.1994","article-title":"Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick blood on filter paper from children born to HIV-1-seropositive mothers","volume":"32","author":"Nyambi","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB17","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1016\/S0035-9203(97)90266-2","article-title":"Comparative evaluation of four serodiagnostic tests for scrub typhus in Thailand","volume":"91","author":"Pradutkanchana","year":"1997","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB18","doi-asserted-by":"crossref","first-page":"900","DOI":"10.4269\/ajtmh.1976.25.900","article-title":"Adaptation of a microimmunofluorescence test to the study of human Rickettsia tsutsugamushi antibody","volume":"25","author":"Robinson","year":"1976","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB19","first-page":"41","article-title":"Detection of Rickettsia tsutsugamushi specific DNA from the lymphocyte of patients by polymerase chain reaction","volume":"19","author":"Shieh","year":"1995","journal-title":"Proc. Natl. Sci. Council, ROC"},{"key":"10.1016\/j.trstmh.2003.10.012_BIB20","doi-asserted-by":"crossref","first-page":"337","DOI":"10.7883\/yoken1952.32.337","article-title":"Antigenic analysis by direct immunofluorescence of 114 isolates of Rickettsia tsutsugamushi recovered from febrile patients in rural Malaysia","volume":"32","author":"Shirai","year":"1979","journal-title":"Jpn. J. Med. Sci. Biol."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB21","first-page":"1","article-title":"Rickettsia tsutsugamushi strains found in chiggers collected in Thailand","volume":"12","author":"Shirai","year":"1981","journal-title":"Southeast Asian J. Trop. Med. Pub. Health"},{"key":"10.1016\/j.trstmh.2003.10.012_BIB22","doi-asserted-by":"crossref","first-page":"9","DOI":"10.7883\/yoken1952.35.9","article-title":"Transmission of scrub typhus to human volunteers by laboratory-reared chiggers","volume":"35","author":"Shirai","year":"1982","journal-title":"Jpn. J. Med. Sci. Biol."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB23","first-page":"794","article-title":"Scrub typhus in the Western Pacific region","volume":"26","author":"Silpapojakul","year":"1997","journal-title":"Ann. Acad. Med. Singapore"},{"key":"10.1016\/j.trstmh.2003.10.012_BIB24","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1016\/0002-9343(54)90262-4","article-title":"Influence of antibiotics on immunologic responses in scrub typhus","volume":"17","author":"Smadel","year":"1954","journal-title":"Am. J. Med."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB25","doi-asserted-by":"crossref","first-page":"636","DOI":"10.4269\/ajtmh.1993.49.636","article-title":"A polymerase chain reaction system for rapid diagnosis of scrub typhus within six hours","volume":"49","author":"Sugita","year":"1993","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.trstmh.2003.10.012_BIB26","first-page":"1368","article-title":"Seronegative tsutsugamushi disease (scrub typhus) diagnosed by polymerase chain reaction","volume":"35","author":"Sugita","year":"1997","journal-title":"Nihon Kyobu Shikkan Gakkai Zasshi"},{"key":"10.1016\/j.trstmh.2003.10.012_BIB27","first-page":"601","article-title":"Serotype-specific amplication of Rickettsia tsutsugamushi DNA from clinical specimen by nested polymerase chain reaction","volume":"68","author":"Yoshida","year":"1994","journal-title":"J. Jpn. Assoc. Infect. Dis."}],"container-title":["Transactions of the Royal Society of Tropical Medicine and Hygiene"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/trstmh\/article-pdf\/98\/6\/360\/5465792\/98-6-360.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:06:19Z","timestamp":1585699579000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/trstmh\/article-lookup\/doi\/10.1016\/j.trstmh.2003.10.012"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,6]]}},"alternative-id":["S0035920304000331"],"URL":"https:\/\/doi.org\/10.1016\/j.trstmh.2003.10.012","relation":{},"ISSN":["0035-9203"],"issn-type":[{"value":"0035-9203","type":"print"}],"subject":[],"published":{"date-parts":[[2004,6]]}}}