{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:15:39Z","timestamp":1791101739603,"version":"4.1.0"},"reference-count":38,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["EDEN0159"],"award-info":[{"award-number":["EDEN0159"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["GOCE-2003-010284 EDEN"],"award-info":[{"award-number":["GOCE-2003-010284 EDEN"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["01KI 0712"],"award-info":[{"award-number":["01KI 0712"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ticks and Tick-borne Diseases"],"published-print":{"date-parts":[[2010,3]]},"DOI":"10.1016\/j.ttbdis.2009.11.002","type":"journal-article","created":{"date-parts":[[2010,2,23]],"date-time":"2010-02-23T08:01:10Z","timestamp":1266912070000},"page":"44-51","source":"Crossref","is-referenced-by-count":72,"title":["Isolation and molecular characterization of a tick-borne encephalitis virus strain from a new tick-borne encephalitis focus with severe cases in Bavaria, Germany"],"prefix":"10.1016","volume":"1","author":[{"given":"Anne M.","family":"Kup\u010da","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Essbauer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gudrun","family":"Zoeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe G.","family":"de Mendon\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Brey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Rinder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kurt","family":"Pfister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Spiegel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bastian","family":"Doerrbecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Pfeffer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Dobler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"Suppl. 1","key":"10.1016\/j.ttbdis.2009.11.002_bib2","first-page":"5","article-title":"The public health importance of tick-borne encephalitis in Europe","volume":"36","author":"Bla\u0161kovi\u010d","year":"1967","journal-title":"Bull. Wld. Hlth Org."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib3","first-page":"275","article-title":"The ecological approach to the study of tick-borne encephalitis","volume":"14","author":"Bla\u0161kovi\u010d","year":"1972","journal-title":"Prog. Med. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib4","first-page":"29","article-title":"Umfassende Beratung \u2013 ausreichender Impfschutz \u2013 sichere Diagnose","volume":"149","author":"Boessenecker","year":"2007","journal-title":"MMW Fortschr. Med."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib5","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1146\/annurev.mi.44.100190.003245","article-title":"Flavivirus genome organization, expression, and replication","volume":"44","author":"Chambers","year":"1990","journal-title":"Annu. Rev. Microbiol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib6","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1023\/A:1020966605960","article-title":"Tick-borne encephalitis virus prevalence in Ixodes ricinus ticks collected in high risk habitats of the South-Bohemian region of the Czech Republic","volume":"26","author":"Danielova","year":"2002","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.jcv.2006.09.001","article-title":"Quality control assessment for the PCR diagnosis of tick-borne encephalitis virus infections","volume":"38","author":"Donoso-Mantke","year":"2007","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib8","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1086\/515195","article-title":"Tick-borne encephalitis","volume":"28","author":"Dumpis","year":"1999","journal-title":"Clin. Inf. Dis."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib9","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1099\/0022-1317-80-1-179","article-title":"Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia","volume":"80","author":"Ecker","year":"1999","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib10","series-title":"The Arboviruses: Epidemiology and Ecology","first-page":"177","article-title":"Tick-borne encephalitis","author":"Gre\u0161ikov\u00e1","year":"1989"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib11","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1099\/0022-1317-82-7-1667","article-title":"The degree of attenuation of tick-borne encephalitis virus depends on the cumulative effects of point mutations","volume":"82","author":"Gritsun","year":"2001","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib12","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0166-3542(02)00206-1","article-title":"Tick-borne encephalitis","volume":"57","author":"Gritsun","year":"2003","journal-title":"Antiviral Res."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib13","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1002\/jmv.1012","article-title":"Prevalence of tick-borne encephalitis virus in Ixodes ricinus ticks in Finland","volume":"64","author":"Han","year":"2001","journal-title":"J. Med. Virol."},{"issue":"Suppl. 1","key":"10.1016\/j.ttbdis.2009.11.002_bib14","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/S0264-410X(02)00820-4","article-title":"Molecular aspects of TBE virus research","volume":"21","author":"Heinz","year":"2003","journal-title":"Vaccine"},{"issue":"Suppl. 1","key":"10.1016\/j.ttbdis.2009.11.002_bib15","first-page":"125","article-title":"The envelope protein E of tick-borne encephalitis virus and other flaviviruses: structure, functions and evolutionary relationships","author":"Heinz","year":"1990","journal-title":"Arch. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib16","series-title":"Ticks of North-West Europe","author":"Hillyard","year":"1996"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib17","doi-asserted-by":"crossref","first-page":"5156","DOI":"10.1128\/jvi.64.10.5156-5159.1990","article-title":"A single amino acid substitution in envelope protein E of tick-borne encephalitis virus leads to attenuation in the mouse model","volume":"64","author":"Holzmann","year":"1990","journal-title":"J. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib18","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1099\/0022-1317-78-1-31","article-title":"Characterization of monoclonal antibody-escape mutants of tick-borne encephalitis virus with reduced neuroinvasiveness in mice","volume":"78","author":"Holzmann","year":"1997","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib19","series-title":"Beitr\u00e4ge zur Geo\u00f6kologie der Zentraleuropaeischen Zecken-Encephalitis","author":"Jusatz","year":"1978"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib20","unstructured":"Knipe, D.M., Howley, P.M., Griffin, D.E., Lamb, R.A., Martin, M.A., Roizman, B., Straus, S.E., 2006. Fields Virology, fifth edition. Vol. 1. Wolters Kluwer, Lippincott, Williams and Wilkins."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib21","first-page":"1","article-title":"Tick-borne encephalitis in Europe","volume":"60","author":"Kunz","year":"1992","journal-title":"Acta Leiden"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib22","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/0168-1702(94)90024-8","article-title":"Change in phenotype of tick-borne encephalitis virus following passage in Ixodes ricinus ticks and associated amino acid substitution in the envelope protein","volume":"31","author":"Labuda","year":"1994","journal-title":"Virus Res."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib23","doi-asserted-by":"crossref","first-page":"5627","DOI":"10.1128\/JVI.75.12.5627-5637.2001","article-title":"Adaptation of tick-borne encephalitis virus to BHK-21 cells results in the formation of multiple heparan sulfate binding sites in the envelope protein and attenuation in vivo","volume":"75","author":"Mandl","year":"2001","journal-title":"J. Virology"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib24","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.virusres.2005.04.007","article-title":"Steps of the tick-borne encephalitis virus replication cycle that affect neuropathogenesis","volume":"111","author":"Mandl","year":"2005","journal-title":"Virus Res."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib25","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1099\/0022-1317-78-11-2711","article-title":"The molecular basis of virulence of the encephalitogenic flaviviruses","volume":"78","author":"McMinn","year":"1997","journal-title":"J. Gen. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib26","series-title":"Fields Virology","first-page":"763","article-title":"Flaviviruses","author":"Monath","year":"1990"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib27","series-title":"Ecological Dynamics of Tick-Borne Zoonoses","first-page":"351","article-title":"Tick-borne Encephalitis Subgroup","author":"Nuttall","year":"1994"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib28","first-page":"357","article-title":"TREEVIEW: an application to display phylogenetic trees on personal computers","volume":"12","author":"Page","year":"1996","journal-title":"Comput. Appl. Biosci."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib29","first-page":"268","article-title":"Nachweis des Virus der Fr\u00fchsommer-Meningoenzephalitis (FSME) in Ixodes ricinus aus dem Kanton Z\u00fcrich, Schweiz","volume":"229","author":"Radda","year":"1974","journal-title":"Zbl. Bakt. Orig. A"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib30","first-page":"148","article-title":"The isolation of central European encephalitis (tick-borne encephalitis) virus from Ixodes ricinus (L.) ticks in Southern Germany","volume":"242","author":"Rehse-K\u00fcpper","year":"1978","journal-title":"Zbl. Bakt., I. Abt. Orig. A"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib31","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1038\/375291a0","article-title":"The envelope glycoprotein from tick-borne encephalitis virus at 2 A resolution","volume":"375","author":"Rey","year":"1995","journal-title":"Nature"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib32","first-page":"165","article-title":"TBE: risk areas in Germany","volume":"18","author":"Robert Koch-Institute","year":"2009","journal-title":"Epidemiol. Bull."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib33","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/0042-6822(89)90509-6","article-title":"Synthetic peptides derived from the deduced amino acid sequence of the E-glycoprotein of Murray Valley encephalitis virus elicit antiviral antibody","volume":"171","author":"Roehrig","year":"1989","journal-title":"Virology"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib34","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/S1386-6532(02)00168-3","article-title":"Development of a quantitative real-time rt-PCR assay with internal control for the laboratory detection of tick borne encephalitis virus (TBEV) RNA","volume":"27","author":"Schwaiger","year":"2003","journal-title":"J. Clin. Virol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib35","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1007\/BF01716096","article-title":"Detection of the tick-borne encephalitits virus (TBEV) in ticks in several federal \"L\u00e4nder\" of Germany by means of the polymerase chain reaction (PCR) \u2013 characterization of the virus","volume":"24","author":"S\u00fcss","year":"1996","journal-title":"Infection"},{"issue":"Suppl. 1","key":"10.1016\/j.ttbdis.2009.11.002_bib36","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1016\/j.ijmm.2008.02.001","article-title":"Genetic characterisation of a tick-borne encephalitis virus isolated from the brain of a naturally exposed monkey (Macaca sylvanus)","volume":"298","author":"S\u00fcss","year":"2008","journal-title":"Int. J. Med. Microbiol."},{"issue":"Suppl. 37","key":"10.1016\/j.ttbdis.2009.11.002_bib37","first-page":"69","article-title":"Tick-borne encephalitis (TBE) in Germany \u2013 epidemiological data, development of risk areas and virus prevalence in field-collected ticks and in ticks removed from humans","volume":"293","author":"S\u00fcss","year":"2004","journal-title":"Int. J. Med. Microbiol."},{"key":"10.1016\/j.ttbdis.2009.11.002_bib38","series-title":"Virus Taxonomy. Eighth Report of the International Committee on the Taxonomy of Viruses","first-page":"981","article-title":"Flaviviridae","author":"Thiel","year":"2005"},{"key":"10.1016\/j.ttbdis.2009.11.002_bib39","doi-asserted-by":"crossref","first-page":"1035","DOI":"10.1099\/0022-1317-77-5-1035","article-title":"Characterization and complete genome sequences of high- and low-virulence variants of tick-borne encephalitis virus","volume":"77","author":"Wallner","year":"1996","journal-title":"J. Gen. Virol."}],"container-title":["Ticks and Tick-borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X09000041?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X09000041?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T20:09:56Z","timestamp":1658088596000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1877959X09000041"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,3]]}},"alternative-id":["S1877959X09000041"],"URL":"https:\/\/doi.org\/10.1016\/j.ttbdis.2009.11.002","relation":{},"ISSN":["1877-959X"],"issn-type":[{"value":"1877-959X","type":"print"}],"subject":[],"published":{"date-parts":[[2010,3]]}}}