{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T00:46:38Z","timestamp":1777596398889,"version":"3.51.4"},"reference-count":41,"publisher":"Elsevier BV","issue":"2","license":[{"start":{"date-parts":[[2017,2,1]],"date-time":"2017-02-01T00:00:00Z","timestamp":1485907200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"CNPq- Instituto Nacional de Ci\u00eancia e Tecnologia de Entomologia Molecular, CAPES (Brazil)"},{"name":"Instituto Nacional de Investigaciones Agropecuarias (INIA) Proyecto","award":["FPTA-270"],"award-info":[{"award-number":["FPTA-270"]}]},{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n (ANII)","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ticks and Tick-borne Diseases"],"published-print":{"date-parts":[[2017,2]]},"DOI":"10.1016\/j.ttbdis.2016.12.008","type":"journal-article","created":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T17:48:18Z","timestamp":1481824098000},"page":"320-329","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular and functional characterization of Bm05br antigen from Rhipicephalus microplus"],"prefix":"10.1016","volume":"8","author":[{"given":"Mar\u00eda Fernanda","family":"Alzugaray","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu\u00eds Fernando","family":"Parizi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Seixas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Uruguaysito","family":"Benavides","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Itabajara","family":"da Silva Vaz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.ttbdis.2016.12.008_bib0005","article-title":"Image processing with imageJ","author":"Abr\u00e0moff","year":"2004","journal-title":"Biophotonics Int."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0010","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1046\/j.1365-2583.2003.00416.x","article-title":"RNA interference in ticks: A study using histamine binding protein dsRNA in the female tick Amblyomma americanum","volume":"12","author":"Aljamali","year":"2003","journal-title":"Insect Mol. Biol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0015","first-page":"250","article-title":"Oviposition of Boophilus microplus (Canestrini) (Acarida: Ixodidae). II. Influence of temperature humidity and light","volume":"16","author":"Bennett","year":"1974","journal-title":"Acarologia"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0020","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/S0020-7519(02)00027-9","article-title":"A cement protein of the tick Rhipicephalus appendiculatus, located in the secretory e cell granules of the type III salivary gland acini, induces strong antibody responses in cattle","volume":"32","author":"Bishop","year":"2002","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0025","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/0169-4758(96)10061-2","article-title":"Tick salivary prostaglandins: presence, origin and significance","volume":"12","author":"Bowman","year":"1996","journal-title":"Parasitol. Today"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0030","doi-asserted-by":"crossref","DOI":"10.1186\/1471-2199-11-74","article-title":"MIQE pr\u00e9cis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments","volume":"11","author":"Bustin","year":"2010","journal-title":"BMC Mol. Biol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0035","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1016\/j.pt.2016.01.004","article-title":"All For One and One For All on the Tick-Host Battlefield","volume":"32","author":"Chmela\u0159","year":"2016","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0040","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1590\/S0100-736X2009000200003","article-title":"First identification of natural infection of Rickettsia rickettsii in the Rhipicephalus sanguineus tick, in the State of Rio de Janeiro. Pesqui","volume":"29","author":"Cunha","year":"2009","journal-title":"Veterin\u00e1ria Bras."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0045","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/S0165-2427(98)00194-9","article-title":"Immunization of bovines with an aspartic proteinase precursor isolated from Boophilus microplus eggs","volume":"66","author":"Da Silva Vaz","year":"1998","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0050","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1586\/14760584.2015.1076339","article-title":"Tick vaccines: current status and future directions","volume":"14","author":"de la Fuente","year":"2015","journal-title":"Expert Rev. Vaccines"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0055","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s11259-007-0069-5","article-title":"Tick vaccines and the transmission of tick-borne pathogens","volume":"31","author":"de la Fuente","year":"2007","journal-title":"Vet. Res. Commun."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0060","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1017\/S0031182010000284","article-title":"Effect of GSK-3 activity, enzymatic inhibition and gene silencing by RNAi on tick oviposition and egg hatching","volume":"137","author":"Fabres","year":"2010","journal-title":"Parasitology"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0065","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.ttbdis.2016.03.018","article-title":"Induction of gene silencing in Haemaphysalis longicornis ticks through immersion in double-stranded RNA","volume":"7","author":"Galay","year":"2016","journal-title":"Ticks Tick. Borne. Dis."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0070","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.vaccine.2008.10.067","article-title":"Characterization of a concealed antigen Hq05 from the hard tick Haemaphysalis qinghaiensis and its effect as a vaccine against tick infestation in sheep","volume":"27","author":"Gao","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0075","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.exppara.2012.02.006","article-title":"Anti-tick monoclonal antibody applied by artificial capillary feeding in Rhipicephalus (Boophilus) microplus females","volume":"130","author":"Gonsioroski","year":"2012","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0080","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1016\/j.ibmb.2005.01.020","article-title":"BmiGI A database of cDNAs expressed in Boophilus microplus, the tropical\/southern cattle tick","volume":"35","author":"Guerrero","year":"2005","journal-title":"Insect Biochem. Mol. Biol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0085","first-page":"310","article-title":"Prediction of N-glycosylation sites in human proteins","volume":"7","author":"Gupta","year":"2002","journal-title":"Pacific Symp. Biocomput."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0090","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1016\/j.ttbdis.2016.01.006","article-title":"Tick iron and heme metabolism \u2212 New target for an anti-tick intervention","volume":"7","author":"Hajdusek","year":"2016","journal-title":"Ticks Tick. Borne. Dis."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0095","unstructured":"Hall, T., 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT. Nucleic Acids Symp. Ser."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0100","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/0735-0651(84)90049-9","article-title":"Improved technique utilizing nonfat dry milk for analysis of proteins and nucleic acids transferred to nitrocellulose","volume":"1","author":"Johnson","year":"1984","journal-title":"Gene Anal. Tech."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0105","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1007\/s00436-007-0483-1","article-title":"Transovarial silencing of the subolesin gene in three-host ixodid tick species after injection of replete females with subolesin dsRNA","volume":"100","author":"Kocan","year":"2007","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0110","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0134779","article-title":"ATP binding cassette transporter mediates both heme and pesticide detoxification in tick midgut cells","volume":"10","author":"Lara","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0120","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1292\/jvms.66.1471","article-title":"Gene Silencing of a Cubilin-Related Serine Proteinase from the Hard Tick Haemaphysalis longicornis by RNA Interference","volume":"66","author":"Miyoshi","year":"2004","journal-title":"J. Vet. Medine Sci."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0125","doi-asserted-by":"crossref","first-page":"W589","DOI":"10.1093\/nar\/gki419","article-title":"dsCheck: Highly sensitive off-target search software for double-stranded RNA-mediated RNA interference","volume":"33","author":"Naito","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0130","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1073\/pnas.0307669100","article-title":"Disruption of Ixodes scapularis anticoagulation by using RNA interference","volume":"101","author":"Narasimhan","year":"2004","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0135","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/protein\/10.1.1","article-title":"Identification of prokaryotic and enkaryotic signal peptides and prediction of their cleavage sites","volume":"10","author":"Nielsen","year":"1997","journal-title":"Protein Eng."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0140","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1016\/j.ijpara.2006.11.005","article-title":"Gene silencing of the tick protective antigens, Bm86, Bm91 and subolesin: in the one-host tick Boophilus microplus by RNA interference","volume":"37","author":"Nijhof","year":"2007","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0145","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1016\/j.ttbdis.2013.06.005","article-title":"Sequence characterization and immunogenicity of cystatins from the cattle tick Rhipicephalus (Boophilus) microplus","volume":"4","author":"Parizi","year":"2013","journal-title":"Ticks Tick. Borne. Dis."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0150","doi-asserted-by":"crossref","first-page":"e45","DOI":"10.1093\/nar\/29.9.e45","article-title":"A new mathematical model for relative quantification in real-time RT\u2013PCR","volume":"29","author":"Pfaffl","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0155","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.1093\/nar\/30.9.e36","article-title":"Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR","volume":"30","author":"Pfaffl","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0160","first-page":"1323","article-title":"ABC transporter efflux pumps: a defense mechanism against ivermectin in Rhipicephalus (Boophilus) microplus","volume":"41","author":"Pohl","year":"2011","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0165","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1007\/s12031-011-9514-6","article-title":"Screening target specificity of siRNAs by rapid amplification of cDNA ends (RACE) for non-sequenced species","volume":"44","author":"Sabirzhanov","year":"2011","journal-title":"J. Mol. Neurosci."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0170","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/S0014-4894(02)00032-2","article-title":"Cloning and partial characterization of a Boophilus microplus calreticulin","author":"Sanchez Ferreira","year":"2002","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0175","doi-asserted-by":"crossref","first-page":"e15926","DOI":"10.1371\/journal.pone.0015926","article-title":"Identification and characterization of Ixodes scapularis antigens that elicit tick immunity using yeast surface display","volume":"6","author":"Schuijt","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0180","doi-asserted-by":"crossref","first-page":"1175","DOI":"10.1016\/j.jinsphys.2008.05.004","article-title":"Effect of 20-hydroxyecdysone and haemolymph on oogenesis in the ixodid tick Amblyomma hebraeum","volume":"54","author":"Seixas","year":"2008","journal-title":"J. Insect Physiol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0185","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.vetimm.2007.10.008","article-title":"Molecular cloning and expression of a larval immunogenic protein from the cattle tick Boophilus annulatus","volume":"121","author":"Shahein","year":"2008","journal-title":"Vet. Immunol. Immunopathol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0190","first-page":"43","article-title":"Differences in the acquired resistance of dogs, hamsters, and guinea","volume":"32","author":"Szah\u00f3","year":"1995","journal-title":"Brazilian J. Vet. Res. Anim. Sci."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0195","doi-asserted-by":"crossref","first-page":"2731","DOI":"10.1093\/molbev\/msr121","article-title":"MEGA5 Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods","volume":"28","author":"Tamura","year":"2011","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0200","doi-asserted-by":"crossref","first-page":"e94831","DOI":"10.1371\/journal.pone.0094831","article-title":"Proteomic analysis of cattle tick Rhipicephalus (Boophilus) microplus saliva: A comparison between partially and fully engorged females","volume":"9","author":"Tirloni","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2016.12.008_bib0205","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/j.vetpar.2006.01.050","article-title":"Tick control: thoughts on a research agenda","volume":"138","author":"Willadsen","year":"2006","journal-title":"Vet. Parasitol."}],"container-title":["Ticks and Tick-borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X16302758?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X16302758?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,7,17]],"date-time":"2022-07-17T20:12:58Z","timestamp":1658088778000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1877959X16302758"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["S1877959X16302758"],"URL":"https:\/\/doi.org\/10.1016\/j.ttbdis.2016.12.008","relation":{},"ISSN":["1877-959X"],"issn-type":[{"value":"1877-959X","type":"print"}],"subject":[],"published":{"date-parts":[[2017,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular and functional characterization of Bm05br antigen from Rhipicephalus microplus","name":"articletitle","label":"Article Title"},{"value":"Ticks and Tick-borne Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ttbdis.2016.12.008","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}