{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T08:31:43Z","timestamp":1782808303087,"version":"3.54.5"},"reference-count":41,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ticks and Tick-borne Diseases"],"published-print":{"date-parts":[[2018,3]]},"DOI":"10.1016\/j.ttbdis.2018.01.013","type":"journal-article","created":{"date-parts":[[2018,2,1]],"date-time":"2018-02-01T17:53:35Z","timestamp":1517507615000},"page":"556-562","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":6,"title":["Comparison of heat shock protein 70\u202fkDa and 18S rDNA genes for molecular detection and phylogenetic analysis of Babesia vogeli from whole blood of naturally infected dogs"],"prefix":"10.1016","volume":"9","author":[{"given":"Patr\u00edcia G.","family":"Paulino","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus S.","family":"Pires","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia B. da","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maristela","family":"Peckle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata L. da","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela L.V.","family":"Vitari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula M. de","family":"Abreu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00e1dia R.P.","family":"Almosny","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos L.","family":"Massard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huarrisson A.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ttbdis.2018.01.013_bib0005","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1292\/jvms.63.111","article-title":"Detection of babesia species from infected dog blood by polymerase chain reaction","volume":"63","author":"Ano","year":"2001","journal-title":"J. Vet. Med. Sci."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0010","series-title":"Sociedade Civil Mamirau\u00e1, Tef\u00e9","article-title":"BioEstat 5.0 \u2013 aplica\u00e7\u00f5es estat\u00edsticas nas \u00c1reas das ci\u00eancias biol\u00f3gicas e M\u00e9dicas","author":"Ayres","year":"2007"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0015","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.vetmic.2008.11.022","article-title":"Longitudinal quantification of Ehrlichia canis in experimental infection with comparison to natural infection","volume":"136","author":"Baneth","year":"2009","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0020","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.ab.2006.06.001","article-title":"Development and evaluation of canine reference genes for accurate quantification of gene expression","volume":"356","author":"Brinkhof","year":"2006","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0025","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"J. Clin. Chem."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0030","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1111\/j.1550-7408.1999.tb05128.x","article-title":"Babesia canis canis, Babesia canis vogeli, Babesia canis rossi: differentiation of the three subspecies by a restriction fragment length polymorphism analysis on amplified small subunit ribosomal RNA genes","volume":"46","author":"Carret","year":"1999","journal-title":"J. Eukrrpor. Microbid."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0035","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.vetpar.2009.06.044","article-title":"Canine piroplasmosis in Italy: epidemiological aspects in vertebrate and invertebrate hosts","volume":"165","author":"Cassini","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0040","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.14202\/vetworld.2015.1370-1374","article-title":"Comparison of molecular and microscopic technique for detection of Theileria annulata from the field cases of cattle","volume":"8","author":"Chauhan","year":"2015","journal-title":"Vet. World"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0045","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1177\/1040638716659101","article-title":"A new quantitative PCR method for the detection of Anaplasma platys in dogs based on the citrate synthase gene","volume":"28","author":"da Silva","year":"2016","journal-title":"J. Vet. Diagn. Investig."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0050","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1128\/JCM.36.3.777-782.1998","article-title":"Detection of cattle naturally infected with Anaplasma marginale in a region of endemicity by nested PCR and a competitive enzyme linked immunosorbentassay using recombinant major surface protein 5","volume":"36","author":"de Echaide","year":"1998","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0055","series-title":"Veterinary PCR Diagnostics","author":"Freeman","year":"2012"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0060","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0166-6851(03)00199-3","article-title":"Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality","volume":"131","author":"Fukumoto","year":"2003","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0065","doi-asserted-by":"crossref","first-page":"986","DOI":"10.1101\/gr.6.10.986","article-title":"Real time quantitative PCR","volume":"6","author":"Heid","year":"1996","journal-title":"Genome Res."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0070","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0166-6851(96)02767-3","article-title":"Single copy Babesia microti hsp70","volume":"83","author":"Jie","year":"1996","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0075","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.ygeno.2017.05.005","article-title":"FastPCR: an in silico tool for fast primer and probe design and advanced sequence analysis","volume":"109","author":"Kalendar","year":"2017","journal-title":"Genomics"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0080","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.vetpar.2007.10.023","article-title":"Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples","volume":"151","author":"Kim","year":"2008","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0085","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1111\/j.1556-4029.2006.00182.x","article-title":"Evaluation of real-time PCR amplification efficiencies to detect PCR inhibitors","volume":"51","author":"Kontanis","year":"2006","journal-title":"J. Forensic Sci."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0090","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.vetpar.2012.09.016","article-title":"Conflicting results of serological, PCR and microscopic methods clarify the various risk levels of canine babesiosis in Slovakia: a complex approach to Babesia canis diagnostics","volume":"191","author":"Kubelov\u00e1","year":"2013","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0095","series-title":"Protozoan Parasites of Domestic Animals and Man","author":"Levine","year":"1943"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0100","first-page":"27","article-title":"Assessment of primers designed from the small ribosomal subunit RNA for specific discrimination between Babesia bigemina and Babesia bovis by PCR","volume":"3","author":"Linhares","year":"2002","journal-title":"Cienc. Anim. Bras."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0105","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.molbiopara.2011.10.006","article-title":"The molecular epidemiology of parasite infections: tools and applications","volume":"181","author":"Lymbery","year":"2012","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0110","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1865-1682.2010.01113.x","article-title":"Development of a loop-mediated isothermal amplification (LAMP) assay for rapid diagnosis of Babesia canis infections","volume":"57","author":"M\u00fcller","year":"2010","journal-title":"Transbound. Emerg. Dis."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0115","unstructured":"Medronho, R., 2009. Epidemiologia. 2\u00aa ed. Atheneu, S\u00e3o Paulo, 676p. Portuguese."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0120","first-page":"315","article-title":"Sobre a natureza do nambiuv\u00fa dos c\u00e3es","volume":"9","author":"Moreira","year":"1938","journal-title":"Arq. Inst. Biol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0125","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1089\/vbz.2016.1990","article-title":"Prevalence and geographic distribution of vector-borne pathogens in apparently healthy dogs in Croatia","volume":"17","author":"Mrljak","year":"2017","journal-title":"Vector-Borne Zoonotic Dis."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0130","first-page":"1","article-title":"Diagnosis of Hepatozoon canis in young dogs by cytology and PCR","volume":"55","author":"Otranto","year":"2011","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0135","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1590\/S0074-02761948000300005","article-title":"Algumas observa\u00e7\u00f5es sobre a babesiose dos c\u00e3es no Rio de Janeiro","volume":"46","author":"Paraense","year":"1948","journal-title":"Mem. Inst. Oswaldo Cruz."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0140","first-page":"347","article-title":"Order piroplasmorida","volume":"1","author":"Peirce","year":"2000"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0145","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.vetpar.2010.06.039","article-title":"Multiplex real time qPCR for the detection of Ehrlichia canis and Babesia canis vogeli","volume":"173","author":"Peleg","year":"2010","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0150","first-page":"163","article-title":"Su di un' infezione del cane con parassiti endoglobulari nel sangue. Nota preventiva","volume":"6","author":"Piana","year":"1895","journal-title":"Modern Zooiatro"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0155","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1093\/bioinformatics\/14.9.817","article-title":"Modeltest: testing the model of DNA substitution","volume":"14","author":"Posada","year":"1998","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0160","doi-asserted-by":"crossref","first-page":"5463","DOI":"10.1073\/pnas.74.12.5463","article-title":"DNA sequencing with chain-terminating inhibitors","volume":"74","author":"Sanger","year":"1977","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0165","doi-asserted-by":"crossref","first-page":"229","DOI":"10.17221\/1946-VETMED","article-title":"Babesiosis as a disease of people and dogs. Molecular diagnostics: a review","volume":"53","author":"Skotarczak","year":"2008","journal-title":"Vet. Med."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0170","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.vetpar.2011.03.024","article-title":"Detection and molecular characterization of a canine piroplasm from Brazil","volume":"180","author":"Soares","year":"2011","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0175","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1186\/s13071-016-1596-0","article-title":"A review of canine babesiosis: the European perspective","volume":"9","author":"Solano-Gallego","year":"2016","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0180","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.bdq.2015.01.005","article-title":"How good is a PCR efficiency estimate: recommendations for precise and robust qPCR efficiency assessments","volume":"3","author":"Svec","year":"2015","journal-title":"Biomol. Detect. Quantif."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0185","doi-asserted-by":"crossref","first-page":"2725","DOI":"10.1093\/molbev\/mst197","article-title":"MEGA 6: molecular evolutionary genetics analysis version 6.0","volume":"30","author":"Tamura","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0190","first-page":"63","article-title":"Altera\u00e7\u00f5es cl\u00ednico-hematol\u00f3gicas da infec\u00e7\u00e3o por Babesia canis vogeli em c\u00e3es do munic\u00edpio de Serop\u00e9dica, Rio de Janeiro","volume":"35","author":"Vilela","year":"2013","journal-title":"Brasil. Rev. Bras. Parasitol. Veterin."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0195","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1038\/11751","article-title":"Detection of PCR products using self-probing amplicons and fluorescence","volume":"17","author":"Whitcombe","year":"1999","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0200","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.vetpar.2007.01.003","article-title":"Comparison and phylogenetic analysis of the heat shock protein 70 gene of Babesia parasites from dogs","volume":"145","author":"Yamasaki","year":"2007","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.01.013_bib0205","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1016\/j.molbiopara.2006.03.015","article-title":"Characterization of the Babesia gibsoni P18 as a homologue of thrombospondin related adhesive protein","volume":"148","author":"Zhou","year":"2006","journal-title":"Mol. Biochem. Parasitol."}],"container-title":["Ticks and Tick-borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X17303655?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X17303655?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,7,15]],"date-time":"2020-07-15T11:34:03Z","timestamp":1594812843000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1877959X17303655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["S1877959X17303655"],"URL":"https:\/\/doi.org\/10.1016\/j.ttbdis.2018.01.013","relation":{},"ISSN":["1877-959X"],"issn-type":[{"value":"1877-959X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Comparison of heat shock protein 70\u202fkDa and 18S rDNA genes for molecular detection and phylogenetic analysis of Babesia vogeli from whole blood of naturally infected dogs","name":"articletitle","label":"Article Title"},{"value":"Ticks and Tick-borne Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ttbdis.2018.01.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}