{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T15:35:38Z","timestamp":1790004938329,"version":"4.0.1"},"reference-count":84,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"H2020 COllaborative Management Platform for detection and Analyses of (Re-) emerging and foodborne outbreaks in Europe","award":["643476"],"award-info":[{"award-number":["643476"]}]},{"name":"Research Plan of the University of Castilla- La Mancha(UCLM), Spain"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ticks and Tick-borne Diseases"],"published-print":{"date-parts":[[2018,7]]},"DOI":"10.1016\/j.ttbdis.2018.04.020","type":"journal-article","created":{"date-parts":[[2018,5,5]],"date-time":"2018-05-05T16:01:49Z","timestamp":1525536109000},"page":"1241-1251","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":50,"title":["Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus"],"prefix":"10.1016","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2198-3611","authenticated-orcid":false,"given":"Ang\u00e9lica","family":"Hern\u00e1ndez-Jargu\u00edn","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"D\u00edaz-S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4172-9079","authenticated-orcid":false,"given":"Margarita","family":"Villar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ttbdis.2018.04.020_bib0005","doi-asserted-by":"crossref","first-page":"E781","DOI":"10.1073\/pnas.1613422114","article-title":"Pathogen-mediated manipulation of arthropod microbiota to promote infection","volume":"114","author":"Abraham","year":"2017","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"Suppl. 1","key":"10.1016\/j.ttbdis.2018.04.020_bib0010","first-page":"5","article-title":"Metagenomics, metatranscriptomics, and metabolomics approaches for microbiome analysis","volume":"12","author":"Aguiar-Pulido","year":"2016","journal-title":"Evol. Bioinform. Online"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0015","doi-asserted-by":"crossref","first-page":"25","DOI":"10.3390\/pathogens3010025","article-title":"Comparative genomics identifies a potential marker of human-virulent Anaplasma phagocytophilum","volume":"3","author":"Al-Khedery","year":"2014","journal-title":"Pathogens"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0020","doi-asserted-by":"crossref","first-page":"2693","DOI":"10.1128\/JCM.28.12.2693-2699.1990","article-title":"Infectious but nonpathogenic isolate of Borrelia burgdorferi","volume":"28","author":"Anderson","year":"1990","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0025","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/1471-2180-11-6","article-title":"Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing","volume":"11","author":"Andreotti","year":"2011","journal-title":"BMC Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0030","doi-asserted-by":"crossref","first-page":"e1005120","DOI":"10.1371\/journal.pgen.1005120","article-title":"Systems biology of tissue-specific response to Anaplasma phagocytophilum reveals differentiated apoptosis in the tick vector Ixodes scapularis","volume":"11","author":"Ayll\u00f3n","year":"2015","journal-title":"PLoS Genet."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0035","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1128\/AEM.70.1.616-620.2004","article-title":"Intracellular symbionts and other bacteria associated with deer ticks (Ixodes scapularis) from Nantucket and Wellfleet Cape Cod, Massachusetts","volume":"70","author":"Benson","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0040","doi-asserted-by":"crossref","first-page":"e2753","DOI":"10.1371\/journal.pntd.0002753","article-title":"Identification of parasitic communities within European ticks using next-generation sequencing","volume":"8","author":"Bonnet","year":"2014","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0045","doi-asserted-by":"crossref","first-page":"236","DOI":"10.3389\/fcimb.2017.00236","article-title":"The tick microbiome: why non-pathogenic microorganisms matter in tick biology and pathogen transmission","volume":"7","author":"Bonnet","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0050","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s10311-016-0581-3","article-title":"Molecular microbiology methods for environmental diagnosis","volume":"14","author":"Bouchez","year":"2016","journal-title":"Environ. Chem. Lett."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0055","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1603\/ME12223","article-title":"An insight into the microbiome of the Amblyomma maculatum (Acari: ixodidae)","volume":"51","author":"Budachetri","year":"2014","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0060","doi-asserted-by":"crossref","first-page":"1186","DOI":"10.1016\/j.ttbdis.2016.07.017","article-title":"Molecular screening for Midichloria in hard and soft ticks reveals variable prevalence levels and bacterial loads in different tick species","volume":"6","author":"Cafiso","year":"2016","journal-title":"Ticks Tick Borne Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0065","doi-asserted-by":"crossref","first-page":"e25604","DOI":"10.1371\/journal.pone.0025604","article-title":"Metagenomic profile of the bacterial communities associated with Ixodes ricinus ticks","volume":"6","author":"Carpi","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0070","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1186\/s40168-017-0375-2","article-title":"MetaLab: an automated pipeline for metaproteomic data analysis","volume":"5","author":"Cheng","year":"2017","journal-title":"Microbiome"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0075","doi-asserted-by":"crossref","first-page":"4371","DOI":"10.1111\/j.1365-294X.2008.03914.x","article-title":"Microbial communities and interactions in the lone star tick Amblyomma americanum","volume":"17","author":"Clay","year":"2008","journal-title":"Mol. Ecol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0080","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1093\/nar\/gkg039","article-title":"The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy","volume":"31","author":"Cole","year":"2003","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0085","doi-asserted-by":"crossref","first-page":"5045","DOI":"10.1128\/AEM.00378-07","article-title":"Molecular characterization and evolution of arthropod-pathogenic Rickettsiella bacteria","volume":"73","author":"Cordaux","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0090","doi-asserted-by":"crossref","first-page":"3164","DOI":"10.1128\/JCM.42.7.3164-3168.2004","article-title":"Multiplex real-time PCR for detection of Anaplasma phagocytophilum and Borrelia burgdorferi","volume":"42","author":"Courtney","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0095","doi-asserted-by":"crossref","first-page":"6938","DOI":"10.2741\/3200","article-title":"Overview: ticks as vectors of pathogens that cause disease in humans and animals","volume":"13","author":"de la Fuente","year":"2008","journal-title":"Front. Biosci."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0100","doi-asserted-by":"crossref","first-page":"114","DOI":"10.3389\/fcimb.2017.00114","article-title":"Tick-pathogen interactions and vector competence: identification of molecular drivers for tick-borne diseases","volume":"7","author":"de la Fuente","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0105","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1038\/nmeth.2227","article-title":"De novo derivation of proteomes from transcriptomes for transcript and protein identification","volume":"9","author":"Evans","year":"2012","journal-title":"Nat. Methods"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0110","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1021\/pr3007842","article-title":"Proteomic analysis of an unculturable bacterial endosymbiont (Blochmannia) reveals high abundance of chaperonins and biosynthetic enzymes","volume":"12","author":"Fan","year":"2013","journal-title":"J. Proteome Res."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0115","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.3201\/eid1907.130005","article-title":"Spotted Fever Group rickettsiae in questing ticks, Central Spain","volume":"19","author":"Fern\u00e1ndez de Mera","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0120","doi-asserted-by":"crossref","first-page":"e00353","DOI":"10.1016\/j.heliyon.2017.e00353","article-title":"Combination of RT-PCR and proteomics for the identification of Crimean-Congo hemorrhagic fever virus in ticks","volume":"3","author":"Fern\u00e1ndez de Mera","year":"2017","journal-title":"Heliyon"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0125","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1128\/microbiolspec.MB-0004-2014","article-title":"Biofilm matrix proteins","volume":"3","author":"Fong","year":"2015","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0130","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1038\/nrmicro3451","article-title":"Sequencing and beyond: integrating molecular \u2018omics' for microbial community profiling","volume":"13","author":"Franzosa","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0135","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1186\/1471-2105-11-80","article-title":"Parameters for accurate genome alignment","volume":"11","author":"Frith","year":"2010","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0140","doi-asserted-by":"crossref","first-page":"e100","DOI":"10.1093\/nar\/gkq010","article-title":"Incorporating sequence quality data into alignment improves DNA read mapping","volume":"38","author":"Frith","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0145","doi-asserted-by":"crossref","first-page":"1846","DOI":"10.1038\/ismej.2015.266","article-title":"The bacterial microbiome of Dermacentor andersoni ticks influences pathogen susceptibility","volume":"10","author":"Gall","year":"2016","journal-title":"ISME J."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0150","first-page":"1095","article-title":"2014. Transcriptome sequence divergence between Lyme disease tick vectors, Ixodes scapularis and Ixodes ricinus","volume":"14","author":"Genomic Resources Development Consortium Contreras M","year":"2014","journal-title":"Mol. Ecol. Resour."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0155","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1186\/s13071-017-2550-5","article-title":"Recent insights into the tick microbiome gained through next-generation sequencing","volume":"11","author":"Greay","year":"2018","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0160","article-title":"Human gut microbiome: function matters","author":"Heintz-Buschart","year":"2017","journal-title":"Trends Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0165","doi-asserted-by":"crossref","first-page":"e79777","DOI":"10.1371\/journal.pone.0079777","article-title":"A Metatranscriptomic approach to the identification of microbiota associated with the ant (Formica exsecta)","volume":"8","author":"Johansson","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0170","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/nature10213","article-title":"Human nutrition, thegut microbiome and the immune system","volume":"474","author":"Kau","year":"2011","journal-title":"Nature"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0175","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1101\/gr.113985.110","article-title":"Adaptive seeds tame genomic sequence comparison","volume":"21","author":"Kie\u0142basa","year":"2011","journal-title":"Genome Res."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0180","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1603\/ME12034","article-title":"Validation of internal reference genes for real-time quantitative polymerase chain reaction studies in the tick Ixodes scapularis (Acari: ixodidae)","volume":"50","author":"Ko\u010di","year":"2013","journal-title":"J. Med. Entomol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0185","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1046\/j.0962-1083.2001.01432.x","article-title":"Prevailing triple infection with Wolbachia in Callosobruchus chinensis (Coleoptera: bruchidae)","volume":"11","author":"Kondo","year":"2002","journal-title":"Mol. Ecol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0190","doi-asserted-by":"crossref","first-page":"e0131413","DOI":"10.1371\/journal.pone.0131413","article-title":"Comparative metagenomic profiling of symbiotic bacterial communities associated with Ixodes persulcatus, Ixodes pavlovskyi and Dermacentor reticulatus ticks","volume":"10","author":"Kurilshikov","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0195","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1093\/nar\/gkr201","article-title":"Interactive Tree Of Life v2: online annotation and display of phylogenetic trees made easy","volume":"39","author":"Letunic","year":"2011","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0200","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/bioinformatics\/btp352","article-title":"1000 genome project data processing subgroup, 2009. the sequence alignment\/map format and SAMtools","volume":"25","author":"Li","year":"2009","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0205","doi-asserted-by":"crossref","first-page":"2987","DOI":"10.1093\/bioinformatics\/btr509","article-title":"A statistical framework for SNP calling, mutation discovery, association mapping and population genetical parameter estimation from sequencing data","volume":"27","author":"Li","year":"2011","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0210","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.ttbdis.2015.10.002","article-title":"Population dynamics of multiple symbionts in the hard tick, Dermacentor silvarum Olenev (Acari: ixodidae)","volume":"7","author":"Liu","year":"2016","journal-title":"Ticks Tick Borne Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0215","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real time quantitative PCR and the 2\u2212\u25b3\u25b3 CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0220","doi-asserted-by":"crossref","first-page":"374","DOI":"10.3389\/fcimb.2017.00374","article-title":"Micropathogen community analysis in Hyalomma rufipes via high-throughput sequencing of small RNAs","volume":"7","author":"Luo","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0225","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.copbio.2010.10.003","article-title":"Comprehensive identification and quantification of microbial transcriptomes by genome-wide unbiased methods","volume":"22","author":"M\u00e4der","year":"2011","journal-title":"Curr. Opin. Biotechnol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0230","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.exppara.2004.11.007","article-title":"Anaplasma platys: an improved PCR for its detection in dogs","volume":"109","author":"Martin","year":"2005","journal-title":"Exp. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0235","doi-asserted-by":"crossref","first-page":"467","DOI":"10.3201\/eid0805.010251","article-title":"Genetic variants of Ehrlichia phagocytophila, Rhode island and connecticut","volume":"8","author":"Massung","year":"2002","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0240","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1086\/379725","article-title":"Inability of a variant strain of Anaplasma phagocytophilum to infect mice","volume":"188","author":"Massung","year":"2003","journal-title":"J. Infect. Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0245","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1111\/j.1462-2920.2005.00955.x","article-title":"Molecular analysis of microbial communities identified in different developmental stages of Ixodes scapularis ticks from Westchester and Dutchess Counties, New York","volume":"8","author":"Moreno","year":"2006","journal-title":"Environ. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0250","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1038\/ismej.2012.171","article-title":"A novel approach, based on BLSOMs (Batch Learning Self- Organizing Maps) to the microbiome analysis of ticks","volume":"7","author":"Nakao","year":"2013","journal-title":"ISME J."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0255","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1016\/j.pt.2015.03.010","article-title":"Tick microbiome: the force within","volume":"31","author":"Narasimhan","year":"2015","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0260","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.chom.2013.12.001","article-title":"Gut microbiota of the tick vector Ixodes scapularis modulate colonization of the Lyme disease spirochete","volume":"15","author":"Narasimhan","year":"2014","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0265","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1038\/s41467-017-00208-0","article-title":"Modulation of the tick gut milieu by a secreted tick protein favors Borrelia burgdorferi colonization","volume":"8","author":"Narasimhan","year":"2017","journal-title":"Nat. Commun."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0270","first-page":"639","article-title":"Design, optimization and evaluation of a polymerase chain reaction for detection of Borrelia spp. Adv","volume":"5","author":"Noda","year":"2013","journal-title":"Clin. Exp. Med."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0275","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1128\/JCM.00366-06","article-title":"Cluster of cases of human Rickettsia felis infection from Southern Europe (Spain) diagnosed by PCR","volume":"44","author":"Oteo","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0280","doi-asserted-by":"crossref","first-page":"789","DOI":"10.1038\/nrmicro3109","article-title":"Going back to the roots: the microbial ecology of the rhizosphere","volume":"11","author":"Philippot","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0285","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1128\/AEM.02987-13","article-title":"Diversity of Rickettsiales in the microbiome of the lone star tick Amblyomma americanum","volume":"80","author":"Ponnusamy","year":"2014","journal-title":"Appl. Env. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0290","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1196\/annals.1355.053","article-title":"Detection of a non-pathogenic variant of Anaplasma phagocytophilum in Ixodes ricinus from La Rioja Spain","volume":"1063","author":"Portillo","year":"2005","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0295","doi-asserted-by":"crossref","first-page":"e103961","DOI":"10.1371\/journal.pone.0103961","article-title":"Microbial population analysis of the salivary glands of ticks: a possible strategy for the surveillance of bacterial pathogens","volume":"9","author":"Qiu","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0300","doi-asserted-by":"crossref","first-page":"e0003929","DOI":"10.1371\/journal.pntd.0003929","article-title":"A comparison between transcriptome sequencing and 16S metagenomics for detection of bacterial pathogens in wildlife","volume":"9","author":"Razzauti","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0305","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1006\/abio.1996.9916","article-title":"Product differentiation by analysis of DNA melting curves during the polymerase chain reaction","volume":"245","author":"Ririe","year":"1997","journal-title":"Anal. Biochem."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0310","doi-asserted-by":"crossref","first-page":"2669","DOI":"10.1128\/AEM.06564-11","article-title":"Factors driving the abundance of Ixodes ricinus ticks and the prevalence of zoonotic I. ricinus-borne pathogens in natural foci","volume":"8","author":"Ruiz-Fons","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0315","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s12915-014-0087-z","article-title":"Reagent and laboratory contamination can critically impact sequence-based microbiome analyses","volume":"12","author":"Salter","year":"2014","journal-title":"BMC Biol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0320","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0167-7012(02)00186-0","article-title":"Application of broad-range 16S rRNA PCR amplification and DGGE fingerprinting for detection of tick-infecting bacteria","volume":"52","author":"Schabereiter-Gurtner","year":"2003","journal-title":"J. Microbiol. Methods"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0325","doi-asserted-by":"crossref","first-page":"2856","DOI":"10.1038\/nprot.2006.468","article-title":"In-gel digestion for mass-spectrometric characterization of proteins and proteomes","volume":"1","author":"Schevchenko","year":"2006","journal-title":"Nat. Protoc."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0330","doi-asserted-by":"crossref","first-page":"800","DOI":"10.1038\/nrc3610","article-title":"The microbiome and cancer","volume":"13","author":"Schwabe","year":"2013","journal-title":"Nat. Rev. Cancer"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0335","doi-asserted-by":"crossref","first-page":"3981","DOI":"10.1128\/AEM.61.11.3981-3985.1995","article-title":"Application of reverse transcriptase PCR for monitoring expression of the catabolic dmpN gene in a phenol-degrading sequencing batch reactor","volume":"61","author":"Selvaratnam","year":"1995","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0340","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1089\/vbz.2016.2006","article-title":"Differentiated THP-1 cells exposed to pathogenic and nonpathogenic Borrelia species demonstrate minimal differences in production of four inflammatory cytokines","volume":"16","author":"Stokes","year":"2016","journal-title":"Vector Borne Zoonotic Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0345","doi-asserted-by":"crossref","first-page":"e82981","DOI":"10.1371\/journal.pone.0082981","article-title":"Evaluating the impact of different sequence databases on metaproteome analysis: insights from a lab-assembled microbial mixture","volume":"8","author":"Tanca","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0350","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1186\/s40168-014-0049-2","article-title":"A straightforward and efficient analytical pipeline for metaproteome characterization","volume":"2","author":"Tanca","year":"2014","journal-title":"Microbiome"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0355","first-page":"1","article-title":"The microbiome of Ehrlichia-infected and uninfected lone star ticks (Amblyomma americanum)","volume":"11","author":"Trout Fryxell","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0360","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1111\/j.1574-6941.2008.00468.x","article-title":"Diversity of Ixodes ricinus tick-associated bacterial communities from different forests","volume":"66","author":"van Overbeek","year":"2008","journal-title":"FEMS Microbiol. Ecol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0365","doi-asserted-by":"crossref","first-page":"6200","DOI":"10.1128\/AEM.01562-15","article-title":"Variation in the microbiota of Ixodes ticks with regard to geography, species, and sex","volume":"81","author":"Van Treuren","year":"2015","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0370","doi-asserted-by":"crossref","first-page":"179","DOI":"10.3389\/fvets.2017.00179","article-title":"Microbial communities in North American ixodid ticks of veterinary and medical importance","volume":"4","author":"Varela-Stokes","year":"2017","journal-title":"Front. Vet. Sci."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0375","doi-asserted-by":"crossref","first-page":"e81439","DOI":"10.1371\/journal.pone.0081439","article-title":"Next deneration sequencing uncovers unexpected bacterial pathogens in ticks in Western Europe","volume":"8","author":"Vayssier-Taussat","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0380","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.2217\/fmb.15.114","article-title":"Emerging horizons for tick-borne pathogens: from the one pathogen-one disease vision to the pathobiome paradigm","volume":"10","author":"Vayssier-Taussat","year":"2015","journal-title":"Future Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0385","doi-asserted-by":"crossref","first-page":"3154","DOI":"10.1074\/mcp.M115.051938","article-title":"Integrated metabolomics, transcriptomics and proteomics identifies metabolic pathways affected by Anaplasma phagocytophilum infection in tick cells","volume":"14","author":"Villar","year":"2015","journal-title":"Mol. Cell. Proteomics."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0390","doi-asserted-by":"crossref","first-page":"e102130","DOI":"10.1371\/journal.pone.0102130","article-title":"Characterization of the bacterial communities of life stages of free living lone star ticks (Amblyomma americanum)","volume":"9","author":"Williams-Newkirk","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0395","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1007\/s10482-016-0780-8","article-title":"Bacteriological analysis of saliva from partially or fully engorged female adult Rhipicephalus microplus by next-generation sequencing","volume":"110","author":"Xiang","year":"2017","journal-title":"Antonie Van Leeuwenhoek"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0400","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s10493-015-9896-1","article-title":"Identification of intestinal bacterial flora in Rhipicephalus microplus ticks by conventional methods and PCR-DGGE analysis","volume":"66","author":"Xu","year":"2015","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0405","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.mib.2015.01.007","article-title":"Functional genomic and metagenomic approaches to understanding gut microbiota-animal mutualism","volume":"24","author":"Yoon","year":"2015","journal-title":"Curr. Opin. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0410","doi-asserted-by":"crossref","first-page":"e90831","DOI":"10.1371\/journal.pone.0090831","article-title":"An efficient strategy of screening for pathogens in wild-caught ticks and mosquitoes by reusing small RNA deep sequencing data","volume":"9","author":"Zhuang","year":"2014","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0415","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1016\/j.ttbdis.2014.04.004","article-title":"Discovery of Rickettsia species in Dermacentor niveus Neumann ticks by investigating the diversity of bacterial communities","volume":"5","author":"Zhuang","year":"2014","journal-title":"Ticks Tick Borne Dis."},{"key":"10.1016\/j.ttbdis.2018.04.020_bib0420","doi-asserted-by":"crossref","first-page":"705034","DOI":"10.1155\/2009\/705034","article-title":"Anaplasma phagocytophilum and Anaplasma marginale elicit different gene expression responses in cultured tick cells","volume":"2009","author":"Zivkovic","year":"2009","journal-title":"Comp. Funct. Genomics"}],"container-title":["Ticks and Tick-borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X18300347?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X18300347?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T16:39:54Z","timestamp":1604248794000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1877959X18300347"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":84,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["S1877959X18300347"],"URL":"https:\/\/doi.org\/10.1016\/j.ttbdis.2018.04.020","relation":{},"ISSN":["1877-959X"],"issn-type":[{"value":"1877-959X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus","name":"articletitle","label":"Article Title"},{"value":"Ticks and Tick-borne Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ttbdis.2018.04.020","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}