{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T17:08:16Z","timestamp":1779901696573,"version":"3.53.1"},"reference-count":40,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2018,7,1]],"date-time":"2018-07-01T00:00:00Z","timestamp":1530403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Italian Ministry of Health","award":["IZS LT 16\/11"],"award-info":[{"award-number":["IZS LT 16\/11"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ticks and Tick-borne Diseases"],"published-print":{"date-parts":[[2018,7]]},"DOI":"10.1016\/j.ttbdis.2018.05.005","type":"journal-article","created":{"date-parts":[[2018,5,4]],"date-time":"2018-05-04T16:54:48Z","timestamp":1525452888000},"page":"1212-1220","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":40,"title":["Genetic diversity of Theileria equi and Babesia caballi infecting horses of Central-Southern Italy and preliminary results of its correlation with clinical and serological status"],"prefix":"10.1016","volume":"9","author":[{"given":"Giuseppe","family":"Manna","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Cersini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4301-7385","authenticated-orcid":false,"given":"Roberto","family":"Nardini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0089-8378","authenticated-orcid":false,"given":"Leticia Elisa","family":"Bartolom\u00e9 Del Pino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Antognetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Zini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raffaella","family":"Conti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raniero","family":"Lorenzetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Veneziano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Luca","family":"Autorino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Teresa","family":"Scicluna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ttbdis.2018.05.005_bib0005","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S1471-4922(02)00065-X","article-title":"Babesiosis: persistence in the face of adversity","volume":"19","author":"Allred","year":"2003","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0010","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1196\/annals.1373.076","article-title":"Molecular sequence evidence for the reclassification of some Babesia species","volume":"1081","author":"Allsopp","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0015","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/j.ttbdis.2016.01.011","article-title":"Babesia caballi and Theileria equi infections in horses in Central-Southern Italy: sero-molecular survey and associated risk factors","volume":"7","author":"Bartolom\u00e9 Del Pino","year":"2016","journal-title":"Ticks Tick. Borne. Dis."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0020","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/S0304-4017(02)00131-0","article-title":"Detection of natural infection of Boophilus microplus with Babesia equi and Babesia caballi in Brazilian horses using nested polymerase chain reaction","volume":"107","author":"Battsetseg","year":"2002","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0025","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/j.vetpar.2008.10.004","article-title":"Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa","volume":"159","author":"Bhoora","year":"2009","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0030","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.vetpar.2010.04.025","article-title":"Sequence heterogeneity in the equi merozoite antigen gene (ema-1) of Theileria equi and development of an ema-1-specific TaqMan MGBTM assay for the detection of T. equi","volume":"172","author":"Bhoora","year":"2010","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0035","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/S0020-7519(00)00105-3","article-title":"The \u03b2-tubulin gene of Babesia and Theileria parasites is an informative marker for species discrimination","volume":"30","author":"Cacci\u00f2","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0040","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1186\/1471-2105-3-2","article-title":"The comparative RNA web (CRW) site: an online database of comparative sequence and structure information for ribosomal, intron, and other RNAs","volume":"3","author":"Cannone","year":"2002","journal-title":"BMC Bioinf."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0045","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1007\/s004360050651","article-title":"A study of the systematics of Theileria spp. based upon small-subunit ribosomal RNA gene sequences","volume":"85","author":"Chae","year":"1999","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0050","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/S0304-4017(03)00141-9","article-title":"Molecular studies on Babesia, Theileria and Hepatozoon in southern Europe: part II. Phylogenetic analysis and evolutionary history","volume":"114","author":"Criado-Fornelio","year":"2003","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0055","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1534\/genetics.107.071399","article-title":"Finely orchestrated movements: evolution of the ribosomal RNA genes","volume":"175","author":"Eickbush","year":"2007","journal-title":"Genetics"},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0060","first-page":"783","article-title":"Confidence limits on phylogenies: an approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution (N.Y)."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0065","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1756-3305-6-35","article-title":"Genetic characterization of Theileria equi infecting horses in North America: evidence for a limited source of U.S. introductions","volume":"6","author":"Hall","year":"2013","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0070","first-page":"275","article-title":"The rapid generation of mutation data matrices from protein sequences","volume":"8","author":"Jones","year":"1992","journal-title":"Comput. Appl. Biosci."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0075","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1186\/1471-2164-13-603","article-title":"Comparative genomic analysis and phylogenetic position of Theileria equi","volume":"13","author":"Kappmeyer","year":"2012","journal-title":"BMC Genomics"},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0080","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/S0166-6851(98)00085-1","article-title":"Phylogenetic analysis of Theileria and Babesia equi in relation to the establishment of parasite populations within novel host species and the development of diagnostic tests","volume":"95","author":"Katzer","year":"1998","journal-title":"Mol. Biochem. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0085","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.vetpar.2007.10.023","article-title":"Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples","volume":"151","author":"Kim","year":"2008","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0090","doi-asserted-by":"crossref","first-page":"2056","DOI":"10.1128\/JCM.29.9.2056-2058.1991","article-title":"Detection of Equine Antibody to Babesia equi Merozoite Proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay","volume":"29","author":"Knowles","year":"1991","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0095","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.meegid.2010.06.008","article-title":"Genetic diversity of equine piroplasms in Greece with a note on speciation within Theileria genotypes (T equi and T. equi-like)","volume":"10","author":"Kouam","year":"2010","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0100","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1016\/j.ijpara.2012.02.005","article-title":"Phylogeny and evolution of the Piroplasmida as inferred from 18S rRNA sequences","volume":"42","author":"Lack","year":"2012","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0105","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1093\/bioinformatics\/btl158","article-title":"Cd-hit: a fast program for clustering and comparing large sets of protein or nucleotide sequences","volume":"22","author":"Li","year":"2006","journal-title":"Bioinformatics"},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0110","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1016\/j.vetpar.2016.03.003","article-title":"Sequence heterogeneity in the 18S rRNA gene in Theileria equi from horses presented in Switzerland","volume":"221","author":"Liu","year":"2016","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0115","doi-asserted-by":"crossref","first-page":"3586","DOI":"10.1128\/JCM.01110-08","article-title":"Preliminary evaluation of the BrEMA-1 gene as a tool for associating Babesia rossi genotypes and clinical manifestation of canine babesiosis","volume":"47","author":"Matjila","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0120","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.pt.2009.05.010","article-title":"Evolution of the Apicomplexa: where are we now?","volume":"25","author":"Morrison","year":"2009","journal-title":"Trends Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0125","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.meegid.2013.02.005","article-title":"Prevalence and genetic diversity of equine piroplasms in Tov province","volume":"16","author":"Munkhjargal","year":"2013","journal-title":"Mongolia. Infect. Genet. Evol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0130","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.vetpar.2004.04.010","article-title":"Detection and identification of equine Theileria and Babesia species by reverse line blotting: epidemiological survey and phylogenetic analysis","volume":"123","author":"Nagore","year":"2004","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0135","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0304-4017(01)00471-X","article-title":"Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction","volume":"101","author":"Nicolaiewsky","year":"2001","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0140","series-title":"Encyclopedia of Arthropod-transmitted Infections of Man and Domesticated Animals","first-page":"487","article-title":"Theilerioses","author":"Preston","year":"2001"},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0145","doi-asserted-by":"crossref","first-page":"1096","DOI":"10.1017\/S0031182013000486","article-title":"Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity","volume":"140","author":"Qablan","year":"2013","journal-title":"Parasitology"},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0150","first-page":"406","article-title":"The neighbor-joining method: a new method for reconstructing phylogenetic trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0155","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/s00436-009-1691-7","article-title":"Nucleotide sequence heterogeneity in the small subunit ribosomal RNA gene within Theileria equi from horses in Sudan","volume":"106","author":"Salim","year":"2010","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0160","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0165702","article-title":"Mitochondrial genome sequences and structures aid in the resolution of Piroplasmida phylogeny","volume":"11","author":"Schreeg","year":"2016","journal-title":"PLoS One"},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0165","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1016\/j.rvsc.2013.01.014","article-title":"Molecular and phylogenetic analysis of equine piroplasms in the Republic of Korea","volume":"94","author":"Seo","year":"2013","journal-title":"Res. Vet. Sci."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0170","doi-asserted-by":"crossref","first-page":"6815","DOI":"10.1073\/pnas.94.13.6815","article-title":"Likelihood-mapping: a simple method to visualize phylogenetic content of a sequence alignment","volume":"94","author":"Strimmer","year":"1997","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0175","doi-asserted-by":"crossref","first-page":"11030","DOI":"10.1073\/pnas.0404206101","article-title":"Prospects for inferring very large phylogenies by using the neighbor-joining method","volume":"101","author":"Tamura","year":"2004","journal-title":"Proc. Natl. Acad. Sci."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0180","first-page":"678","article-title":"Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G\u202f+\u202fC-content biases","volume":"9","author":"Tamura","year":"1992","journal-title":"Mol. Biol. Evol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0185","doi-asserted-by":"crossref","first-page":"5803","DOI":"10.1128\/JCM.41.12.5803-5809.2003","article-title":"Expression of Equi Merozoite Antigen 2 during development of Babesia equi in the midgut and salivary gland of the vector tick Boophilus microplus","volume":"41","author":"Ueti","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0190","series-title":"Encyclopedia of Arthropod-transmitted Infections of Man and Domesticated Animals","first-page":"53","article-title":"Babesiosis","author":"Uilenberg","year":"2001"},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0195","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1016\/j.vetmic.2014.08.017","article-title":"Molecular and serological detection of tick-borne pathogens in donkeys (Equus asinus) in Italy","volume":"173","author":"Veronesi","year":"2014","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.ttbdis.2018.05.005_bib0200","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1016\/0007-1935(92)90061-5","article-title":"Equine piroplasmosis: a review","volume":"148","author":"de Waal","year":"1992","journal-title":"Br. Vet. J."}],"container-title":["Ticks and Tick-borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X17304168?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X17304168?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,11,1]],"date-time":"2020-11-01T16:06:23Z","timestamp":1604246783000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1877959X17304168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7]]},"references-count":40,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,7]]}},"alternative-id":["S1877959X17304168"],"URL":"https:\/\/doi.org\/10.1016\/j.ttbdis.2018.05.005","relation":{},"ISSN":["1877-959X"],"issn-type":[{"value":"1877-959X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Genetic diversity of Theileria equi and Babesia caballi infecting horses of Central-Southern Italy and preliminary results of its correlation with clinical and serological status","name":"articletitle","label":"Article Title"},{"value":"Ticks and Tick-borne Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ttbdis.2018.05.005","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}]}}