{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T17:10:29Z","timestamp":1793121029226,"version":"4.5.0"},"reference-count":53,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Ticks and Tick-borne Diseases"],"published-print":{"date-parts":[[2021,1]]},"DOI":"10.1016\/j.ttbdis.2020.101583","type":"journal-article","created":{"date-parts":[[2020,10,10]],"date-time":"2020-10-10T23:32:54Z","timestamp":1602372774000},"page":"101583","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":31,"title":["Molecular identification and characterization of Rickettsia spp. and other tick-borne pathogens in cattle and their ticks from Huambo, Angola"],"prefix":"10.1016","volume":"12","author":[{"given":"Patr\u00edcia F.","family":"Barradas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o R.","family":"Mesquita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1tima","family":"G\u00e4rtner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madalena","family":"Carvalho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7218-4668","authenticated-orcid":false,"given":"Esmeralda","family":"In\u00e1cio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Chivinda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f3nio","family":"Katimba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Amorim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.ttbdis.2020.101583_bib0005","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1186\/s13071-019-3699-x","article-title":"Molecular identification and prevalence of tick-borne pathogens in zebu and taurine cattle in North Cameroon","volume":"12","author":"Abanda","year":"2019","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0010","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1089\/vbz.2010.0241","article-title":"Molecular detection of spotted fever group rickettsiae associated with ixodid ticks in Egypt","volume":"12","author":"Abdel-Shafy","year":"2012","journal-title":"Vector Borne Zoonotic Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0015","first-page":"137","article-title":"Identification and genetic characterization of Piroplasmida and Anaplasmataceae agents in feeding Amblyomma variegatum ticks from Benin","volume":"14","author":"Adjou Moumouni","year":"2018","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0020","doi-asserted-by":"crossref","first-page":"1161","DOI":"10.4269\/ajtmh.14-0372","article-title":"Case report: molecular evidence of Anaplasma platys infection in two women from Venezuela","volume":"91","author":"Arraga-Alvarado","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0025","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.meegid.2017.01.021","article-title":"Genetic diversity and molecular epidemiology of Anaplasma","volume":"49","author":"Battilani","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0030","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.meegid.2017.01.023","article-title":"Anaplasma platys-like strains in ruminants from Tunisia","volume":"49","author":"Ben Said","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0035","doi-asserted-by":"crossref","first-page":"S271","DOI":"10.1017\/S0031182003004748","article-title":"Theileria: intracellular protozoan parasites of wild and domestic ruminants transmitted by ixodid ticks","volume":"129","author":"Bishop","year":"2004","journal-title":"Parasitology"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0040","doi-asserted-by":"crossref","first-page":"10034","DOI":"10.1073\/pnas.91.21.10034","article-title":"Phylogeny of hard- and soft-tick taxa (Acari: ixodida) based on mitochondrial 16S rDNA sequences","volume":"91","author":"Black","year":"1994","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0045","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1186\/1756-3305-7-237","article-title":"Tick-borne pathogens and associated co-infections in ticks collected from domestic animals in central China","volume":"7","author":"Chen","year":"2014","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0050","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1128\/CDLI.12.6.759-763.2005","article-title":"Evaluation of PCR-based assay for diagnosis of spotted fever group rickettsiosis in human serum samples","volume":"12","author":"Choi","year":"2005","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0055","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s10493-018-00336-2","article-title":"First molecular detection of Rickettsia africae in a tropical bont tick, Amblyomma variegatum, collected in Corsica, France","volume":"77","author":"Cicculli","year":"2019","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0060","first-page":"4","article-title":"Experiences in tick control by acaricide in the traditional cattle sector in Zambia and Burkina Faso: possible environmental and public health implications","author":"De Meneghi","year":"2016","journal-title":"Front. Public Health"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0065","doi-asserted-by":"crossref","first-page":"1828","DOI":"10.3201\/eid1112.050898","article-title":"Human granulocytic anaplasmosis and Anaplasma phagocytophilum","volume":"11","author":"Dumler","year":"2005","journal-title":"Emerg. Infect Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0070","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1089\/vbz.2010.0056","article-title":"Cattle and the natural history of Rickettsia parkeri in Mississippi","volume":"11","author":"Edwards","year":"2011","journal-title":"Vector Borne Zoonotic Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0075","doi-asserted-by":"crossref","DOI":"10.1007\/s11250-019-02176-7","article-title":"A molecular prevalence survey on Anaplasma infection among domestic ruminants in Khartoum State, Sudan","author":"Eisawi","year":"2020","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0080","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s10493-007-9050-9","article-title":"Using invaded range data to model the climate suitability for Amblyomma variegatum (Acari: ixodidae) in the New World","volume":"41","author":"Estrada-Pena","year":"2007","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0085","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.tvjl.2007.01.013","article-title":"Detection of Ehrlichia canis by PCR in different tissues obtained during necropsy from dogs surveyed for naturally occurring canine monocytic ehrlichiosis","volume":"175","author":"Gal","year":"2008","journal-title":"Vet. J."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0090","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1016\/j.ttbdis.2019.04.008","article-title":"Genetic characterization of tick-borne pathogens in ticks infesting cattle and sheep from three South African provinces","volume":"10","author":"Guo","year":"2019","journal-title":"Ticks Tick. Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0095","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1128\/CMR.13.3.451","article-title":"Babesiosis","volume":"13","author":"Homer","year":"2000","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0100","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1017\/S0031182004005967","article-title":"The global importance of ticks","volume":"129","author":"Jongejan","year":"2004","journal-title":"Parasitology"},{"issue":"Suppl","key":"10.1016\/j.ttbdis.2020.101583_bib0105","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1017\/S0031182004005967","article-title":"The global importance of ticks","volume":"129","author":"Jongejan","year":"2004","journal-title":"Parasitology"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0110","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1128\/AEM.72.2.1102-1109.2006","article-title":"Novel genetic variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a novel Ehrlichia sp. in wild deer and ticks on two major islands in Japan","volume":"72","author":"Kawahara","year":"2006","journal-title":"Appl. Environ. Microbiol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0115","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1099\/00207713-46-2-611","article-title":"Rickettsia africae sp. nov., the etiological agent of African tick bite fever","volume":"46","author":"Kelly","year":"1996","journal-title":"Int. J. Syst. Bacteriol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0120","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s11250-006-4181-2","article-title":"Estimated direct economic costs associated with tick-borne diseases on cattle in Tanzania","volume":"38","author":"Kivaria","year":"2006","journal-title":"Trop. Anim. Health Prod."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0125","first-page":"417","volume":"19","author":"Kubelov\u00e1","year":"2012"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0130","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/S1473-3099(15)70051-4","article-title":"Human infection with a novel tick-borne Anaplasma species in China: a surveillance study","volume":"15","author":"Li","year":"2015","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0135","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.3201\/eid1910.130389","article-title":"Rickettsia africae in Amblyomma variegatum ticks, Uganda and Nigeria","volume":"19","author":"Lorusso","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0140","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s10493-017-0155-5","article-title":"Molecular detection of pathogens in ticks infesting cattle in Nampula province, Mozambique","volume":"73","author":"Matsimbe","year":"2017","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0145","doi-asserted-by":"crossref","first-page":"571","DOI":"10.3201\/eid1603.090346","article-title":"Rickettsia africae, Western Africa","volume":"16","author":"Mediannikov","year":"2010","journal-title":"Emerg. Infect Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0150","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1007\/s00436-018-5775-0","article-title":"Molecular detection and characterization of Theileria spp. infecting cattle in Sennar State, Sudan","volume":"117","author":"Mohamed","year":"2018","journal-title":"Parasitol. Res."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0155","doi-asserted-by":"crossref","first-page":"432","DOI":"10.3201\/eid1003.020713","article-title":"Acute spotted fever rickettsiosis among febrile patients, Cameroon","volume":"10","author":"Ndip","year":"2004","journal-title":"Emerg. Infect Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0160","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.vetpar.2012.01.029","article-title":"Molecular identification of tick-borne pathogens in Nigerian ticks","volume":"187","author":"Ogo","year":"2012","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0165","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0001-706X(97)00045-4","article-title":"A subtropical case of human babesiosis","volume":"67","author":"Olmeda","year":"1997","journal-title":"Acta Trop."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0170","doi-asserted-by":"crossref","first-page":"897","DOI":"10.1086\/319347","article-title":"Ticks and tickborne bacterial diseases in humans: an emerging infectious threat","volume":"32","author":"Parola","year":"2001","journal-title":"Clin. Infect. Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0175","doi-asserted-by":"crossref","first-page":"719","DOI":"10.1128\/CMR.18.4.719-756.2005","article-title":"Tick-borne rickettsioses around the world: emerging diseases challenging old concepts","volume":"18","author":"Parola","year":"2005","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0180","doi-asserted-by":"crossref","first-page":"657","DOI":"10.1128\/CMR.00032-13","article-title":"Update on tick-borne rickettsioses around the world: a geographic approach","volume":"26","author":"Parola","year":"2013","journal-title":"Clin. Microbiol. Rev."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0185","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1016\/S0020-7519(00)00141-7","article-title":"Effects of environmental change on emerging parasitic diseases","volume":"30","author":"Patz","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0190","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1525\/bio.2009.59.11.6","article-title":"Biodiversity loss affects global disease ecology","volume":"59","author":"Pongsiri","year":"2009","journal-title":"BioScience"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0195","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.1128\/JB.173.5.1576-1589.1991","article-title":"Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes","volume":"173","author":"Regnery","year":"1991","journal-title":"J. Bacteriol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0200","doi-asserted-by":"crossref","first-page":"1437","DOI":"10.1016\/j.ttbdis.2018.06.014","article-title":"Molecular detection and characterization of tick-borne protozoan and rickettsial pathogens isolated from cattle on Pemba Island, Tanzania","volume":"9","author":"Ringo","year":"2018","journal-title":"Ticks Tick. Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0205","doi-asserted-by":"crossref","first-page":"251","DOI":"10.3389\/fpubh.2014.00251","article-title":"Ixodes ricinus and its transmitted pathogens in urban and peri-urban areas in Europe: new hazards and relevance for public health","volume":"2","author":"Rizzoli","year":"2014","journal-title":"Front. Public Health"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0210","first-page":"101330","article-title":"Molecular identification of protozoal and bacterial organisms in domestic animals and their infesting ticks from north-eastern Algeria","author":"Sadeddine","year":"2019","journal-title":"Ticks Tick. Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0215","doi-asserted-by":"crossref","first-page":"2215","DOI":"10.1128\/JCM.37.7.2215-2222.1999","article-title":"Detection and identification of Ehrlichia, Borrelia burgdorferi sensu lato, and Bartonella species in Dutch Ixodes ricinus ticks","volume":"37","author":"Schouls","year":"1999","journal-title":"J. Clin. Microbiol."},{"issue":"Suppl 2","key":"10.1016\/j.ttbdis.2020.101583_bib0220","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1111\/j.1469-0691.2008.02256.x","article-title":"Detection of Rickettsia aeschlimannii in Hyalomma marginatum ticks in western Russia","volume":"15","author":"Shpynov","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"issue":"Suppl 2","key":"10.1016\/j.ttbdis.2020.101583_bib0225","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1111\/j.1469-0691.2008.02257.x","article-title":"Transmission of Rickettsia conorii conorii in naturally infected Rhipicephalus sanguineus","volume":"15","author":"Socolovschi","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0230","first-page":"130","article-title":"Biological and DNA evidence of two dissimilar populations of the Rhipicephalus sanguineus tick group (Acari: ixodidae) in South America","author":"Szab\u00f3","year":"2005","journal-title":"Vet. Parasitol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0235","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.ttbdis.2018.02.004","article-title":"Occurrence of tick-borne haemoparasites in cattle in the Mungwi District, Northern Province, Zambia","volume":"9","author":"Tembo","year":"2018","journal-title":"Ticks Tick. Dis."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0240","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s10493-012-9528-y","article-title":"Ticks and tick-borne pathogens in livestock from nomadic herds in the Somali Region, Ethiopia","volume":"56","author":"Tomassone","year":"2012","journal-title":"Exp. Appl. Acarol."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0245","series-title":"Ticks of Domestic Animals in Africa: a Guide to Identification of Species","author":"Walker","year":"2014"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0250","unstructured":"https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/vector-borne-diseases https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/vector-borne-diseases (accessed 03 March 2020)."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0255","doi-asserted-by":"crossref","first-page":"446","DOI":"10.1196\/annals.1374.084","article-title":"Anaplasma phagocytophilum in ruminants in Europe","volume":"1078","author":"Woldehiwet","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"10.1016\/j.ttbdis.2020.101583_bib0260","doi-asserted-by":"crossref","first-page":"603","DOI":"10.1186\/s13071-016-1886-6","article-title":"Molecular survey and characterization of a novel Anaplasma species closely related to Anaplasma capra in ticks, northwestern China","volume":"9","author":"Yang","year":"2016","journal-title":"Parasit. Vectors"},{"key":"10.1016\/j.ttbdis.2020.101583_bib0265","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1186\/s13071-017-2182-9","article-title":"A novel zoonotic Anaplasma species is prevalent in small ruminants: potential public health implications","volume":"10","author":"Yang","year":"2017","journal-title":"Parasit. Vectors"}],"container-title":["Ticks and Tick-borne Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X20304520?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1877959X20304520?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,4,2]],"date-time":"2021-04-02T14:30:30Z","timestamp":1617373830000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1877959X20304520"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1]]},"references-count":53,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1]]}},"alternative-id":["S1877959X20304520"],"URL":"https:\/\/doi.org\/10.1016\/j.ttbdis.2020.101583","relation":{},"ISSN":["1877-959X"],"issn-type":[{"value":"1877-959X","type":"print"}],"subject":[],"published":{"date-parts":[[2021,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular identification and characterization of Rickettsia spp. and other tick-borne pathogens in cattle and their ticks from Huambo, Angola","name":"articletitle","label":"Article Title"},{"value":"Ticks and Tick-borne Diseases","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.ttbdis.2020.101583","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier GmbH. All rights reserved.","name":"copyright","label":"Copyright"}],"article-number":"101583"}}