{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T17:45:06Z","timestamp":1777484706506,"version":"3.51.4"},"reference-count":33,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"MinCyT UE Mercosur grant BiotechSur","award":["AERG 232001"],"award-info":[{"award-number":["AERG 232001"]}]},{"name":"MinCyT UE Mercosur grant BiotechSur","award":["AEBIO243512"],"award-info":[{"award-number":["AEBIO243512"]}]},{"name":"MinCyT UE Mercosur grant BiotechSur","award":["PICT2324"],"award-info":[{"award-number":["PICT2324"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Tuberculosis"],"published-print":{"date-parts":[[2013,5]]},"DOI":"10.1016\/j.tube.2013.02.004","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T03:52:47Z","timestamp":1363665167000},"page":"363-372","source":"Crossref","is-referenced-by-count":34,"title":["Mycobacterium bovis \u0394mce2 double deletion mutant protects cattle against challenge with virulent M.\u00a0bovis"],"prefix":"10.1016","volume":"93","author":[{"given":"Federico Carlos","family":"Blanco","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Ver\u00f3nica","family":"Bianco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Garbaccio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia","family":"Meikle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jos\u00e9","family":"Gravisaco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Montenegro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar","family":"Alfonseca","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahavir","family":"Singh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soledad","family":"Barandiaran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Canal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucas","family":"Vagnoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryce Malcom","family":"Buddle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabiana","family":"Bigi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angel","family":"Cataldi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.tube.2013.02.004_bib1","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.tube.2006.01.007","article-title":"The situation of tuberculosis and tuberculosis control in animals of economic interest","volume":"86","author":"Amanfu","year":"2006","journal-title":"Tuberculosis (Edinb)"},{"key":"10.1016\/j.tube.2013.02.004_bib2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.vetmic.2005.11.033","article-title":"An update on bovine tuberculosis programmes in Latin American and Caribbean countries","volume":"112","author":"de Kantor","year":"2006","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.tube.2013.02.004_bib3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.vetmic.2011.02.021","article-title":"Update on vaccination of cattle and wildlife populations against tuberculosis","volume":"151","author":"Buddle","year":"2011","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.tube.2013.02.004_bib4","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1016\/j.vaccine.2012.02.018","article-title":"Bovine tuberculosis vaccine research: historical perspectives and recent advances","volume":"30","author":"Waters","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.tube.2013.02.004_bib5","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.vaccine.2005.07.078","article-title":"Preclinical testing of new vaccines for tuberculosis: a comprehensive review","volume":"24","author":"Orme","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.tube.2013.02.004_bib6","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/j.micinf.2005.04.001","article-title":"Live attenuated mutants of Mycobacterium tuberculosis as candidate vaccines against tuberculosis","volume":"7","author":"Sambandamurthy","year":"2005","journal-title":"Microbe Infect"},{"key":"10.1016\/j.tube.2013.02.004_bib7","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1517\/14712598.8.2.201","article-title":"Live tuberculosis vaccines based on phoP mutants: a step towards clinical trials","volume":"8","author":"Asensio","year":"2008","journal-title":"Expert Opin Biol Ther"},{"key":"10.1016\/j.tube.2013.02.004_bib8","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.tube.2006.01.022","article-title":"The use of mutant mycobacteria as new vaccines to prevent tuberculosis","volume":"86","author":"Hern\u00e1ndez Pando","year":"2006","journal-title":"Tuberculosis (Edinb)"},{"key":"10.1016\/j.tube.2013.02.004_bib9","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1126\/science.8367727","article-title":"Cloning of an M.\u00a0tuberculosis DNA fragment associated with entry and survival inside cells","volume":"261","author":"Arruda","year":"1993","journal-title":"Science"},{"key":"10.1016\/j.tube.2013.02.004_bib10","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1016\/j.vaccine.2005.11.051","article-title":"Immunogenicity and protection induced by Mycobacterium tuberculosis mce-2 and mce-3 mutants in a Balb\/c mouse model of progressive pulmonary tuberculosis","volume":"24","author":"Aguilar","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.tube.2013.02.004_bib11","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.micinf.2004.11.007","article-title":"Mutation in mce operons attenuates Mycobacterium tuberculosis virulence","volume":"7","author":"Gioffre","year":"2005","journal-title":"Microbe Infect"},{"key":"10.1016\/j.tube.2013.02.004_bib12","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1016\/j.micinf.2007.05.020","article-title":"Enhanced mortality despite control of lung infection in mice aerogenically infected with a Mycobacterium tuberculosis mce1 operon mutant","volume":"9","author":"Lima","year":"2007","journal-title":"Microbe Infect"},{"key":"10.1016\/j.tube.2013.02.004_bib13","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.tube.2009.10.004","article-title":"Mce2 operon mutant strain of Mycobacterium tuberculosis is attenuated in C57BL\/6 mice","volume":"90","author":"Marjanovic","year":"2010","journal-title":"Tuberculosis (Edinb)"},{"key":"10.1016\/j.tube.2013.02.004_bib14","first-page":"111","article-title":"Good protection but excessive pulmonary inflammation in Balb\/C mice vaccinated with mycobacterium bovis mce-2A mutant after challenge with homologous strains","volume":"2","author":"Alfonseca-Silva","year":"2012","journal-title":"Mycobac Dis"},{"key":"10.1016\/j.tube.2013.02.004_bib15","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1099\/jmm.0.47454-0","article-title":"Mycobacterium tuberculosis strains disrupted in mce3 and mce4 operons are attenuated in mice","volume":"57","author":"Senaratne","year":"2008","journal-title":"J\u00a0Med Microbiol"},{"key":"10.1016\/j.tube.2013.02.004_bib16","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1365-2249.2009.03923.x","article-title":"Mycobacterium bovis with different genotypes and from different hosts induce dissimilar immunopathological lesions in a mouse model of tuberculosis","volume":"157","author":"Aguilar Le\u00f3n","year":"2009","journal-title":"Clin Exp Immunol"},{"key":"10.1016\/j.tube.2013.02.004_bib17","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/1746-6148-7-37","article-title":"Evaluation of pathogenesis caused in cattle and guinea pig by a Mycobacterium bovis strain isolated from wild boar","volume":"7","author":"Meikle","year":"2011","journal-title":"BMC Vet Res"},{"key":"10.1016\/j.tube.2013.02.004_bib18","first-page":"258353","article-title":"Assessment of the immune responses induced in cattle after inoculation of a Mycobacterium bovis strain deleted in two mce2 genes","volume":"2012","author":"Blanco","year":"2012","journal-title":"J\u00a0Biomed Biotechnol"},{"key":"10.1016\/j.tube.2013.02.004_bib19","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1128\/CVI.00034-08","article-title":"Enhanced protection against bovine tuberculosis after coadministration of Mycobacterium bovis BCG with a mycobacterial protein vaccine-adjuvant combination but not after coadministration of adjuvant alone","volume":"15","author":"Wedlock","year":"2008","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.tube.2013.02.004_bib20","doi-asserted-by":"crossref","first-page":"460","DOI":"10.1111\/j.1348-0421.2009.00141.x","article-title":"Study of the immunological profile towards Mycobacterium bovis antigens in naturally infected cattle","volume":"53","author":"Blanco","year":"2009","journal-title":"Microbiol Immunol"},{"key":"10.1016\/j.tube.2013.02.004_bib21","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.1093\/nar\/30.9.e36","article-title":"Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR","volume":"30","author":"Pfaffl","year":"2002","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.tube.2013.02.004_bib22","series-title":"\u201cBacteriolog\u00eda de la tuberculosis Ns11, \u201dCEPANZO OPS\/OMS","author":"de Kantor","year":"1988"},{"key":"10.1016\/j.tube.2013.02.004_bib23","doi-asserted-by":"crossref","first-page":"2232","DOI":"10.1016\/j.jcpa.2005.05.001","article-title":"Advanced granulomatous lesions in Mycobacterium bovis-infected cattle are associated with increased expression of type I procollagen, gammadelta (WC1+) T cells and CD 68+ cells","volume":"133","author":"Wangoo","year":"2005","journal-title":"J\u00a0Comp Pathol"},{"key":"10.1016\/j.tube.2013.02.004_bib24","doi-asserted-by":"crossref","first-page":"3364","DOI":"10.1128\/IAI.00287-09","article-title":"Viral booster vaccines improve Mycobacterium bovis BCG-induced protection against bovine tuberculosis","volume":"77","author":"Vordermeier","year":"2009","journal-title":"Infect Immun"},{"key":"10.1016\/j.tube.2013.02.004_bib25","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/j.vaccine.2008.12.018","article-title":"Efficacy and immunogenicity of Mycobacterium bovis \u0394RD1 against aerosol M.\u00a0bovis infection in neonatal calves","volume":"27","author":"Waters","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.tube.2013.02.004_bib26","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.vetimm.2007.04.005","article-title":"Vaccination of cattle with Danish and Pasteur strains of Mycobacterium bovis BCG induce different levels of IFNgamma post-vaccination, but induce similar levels of protection against bovine tuberculosis","volume":"118","author":"Wedlock","year":"2007","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.tube.2013.02.004_bib27","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/j.tube.2010.11.007","article-title":"Increased IL-17 expression is associated with pathology in a bovine model of tuberculosis","volume":"91","author":"Blanco","year":"2011","journal-title":"Tuberculosis (Edinb)"},{"key":"10.1016\/j.tube.2013.02.004_bib28","doi-asserted-by":"crossref","first-page":"2200","DOI":"10.1002\/eji.201040433","article-title":"Enhanced protection to Mycobacterium tuberculosis infection in IL-10-deficient mice is accompanied by early and enhanced Th1 responses in the lung","volume":"40","author":"Redford","year":"2010","journal-title":"Eur J Immunol"},{"key":"10.1016\/j.tube.2013.02.004_bib29","doi-asserted-by":"crossref","first-page":"4414","DOI":"10.4049\/jimmunol.0903332","article-title":"Essential role of IL-17A in the formation of a mycobacterial infection induced granuloma in the lung","volume":"184","author":"Okamoto Yoshida","year":"2010","journal-title":"J\u00a0Immunol"},{"key":"10.1016\/j.tube.2013.02.004_bib30","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1016\/j.vetimm.2007.05.009","article-title":"Associations between cytokine gene expression and pathology in Mycobacterium bovis infected cattle","volume":"119","author":"Thacker","year":"2007","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.tube.2013.02.004_bib31","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1128\/JB.00750-10","article-title":"African 2, a clonal complex of Mycobacterium bovis epidemiologically important in East Africa","volume":"193","author":"Berg","year":"2011","journal-title":"J\u00a0Bacteriol"},{"key":"10.1016\/j.tube.2013.02.004_bib32","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.tube.2003.08.010","article-title":"Immunology of tuberculosis and implications in vaccine development","volume":"84","author":"Flynn","year":"2004","journal-title":"Tuberculosis (Edinb)"},{"key":"10.1016\/j.tube.2013.02.004_bib33","doi-asserted-by":"crossref","first-page":"3176","DOI":"10.1128\/JCM.00420-10","article-title":"Development of a skin test for bovine tuberculosis for differentiating infected from vaccinated animals","volume":"48","author":"Whelan","year":"2010","journal-title":"J\u00a0Clin Microbiol"}],"container-title":["Tuberculosis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1472979213000127?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1472979213000127?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T23:23:40Z","timestamp":1539818620000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1472979213000127"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["S1472979213000127"],"URL":"https:\/\/doi.org\/10.1016\/j.tube.2013.02.004","relation":{},"ISSN":["1472-9792"],"issn-type":[{"value":"1472-9792","type":"print"}],"subject":[],"published":{"date-parts":[[2013,5]]}}}