{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:16:12Z","timestamp":1792800972556,"version":"4.5.0"},"reference-count":34,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2006,5,1]],"date-time":"2006-05-01T00:00:00Z","timestamp":1146441600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003198","name":"Minist\u00e8re de l\u2019Agriculture, de l\u2019Agroalimentaire et de la For\u00eat","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005989","name":"Ministrstvo za Izobra\u017eevanje, Znanost in \u0160port","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005989","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Veterinary Journal"],"published-print":{"date-parts":[[2006,5]]},"DOI":"10.1016\/j.tvjl.2004.11.004","type":"journal-article","created":{"date-parts":[[2005,1,11]],"date-time":"2005-01-11T07:38:59Z","timestamp":1105429139000},"page":"539-544","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular detection of BHV-1 in artificially inoculated semen and in the semen of a latently infected bull treated with dexamethasone"],"prefix":"10.1016","volume":"171","author":[{"given":"Jo\u017ee","family":"Grom","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Hostnik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivan","family":"Toplak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darja","family":"Barli\u010d-Maganja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tvjl.2004.11.004_bib1","first-page":"93","article-title":"Viruses associated with bovine semen","volume":"60","author":"Afshar","year":"1990","journal-title":"Veterinary Bulletin"},{"key":"10.1016\/j.tvjl.2004.11.004_bib2","first-page":"21","article-title":"Clinical and serological follow up of young calves experimentally infected with virulent bovine herpesvirus (BHV-1)","volume":"45","author":"Brenner","year":"1989","journal-title":"Israel Journal of Veterinary Medicine"},{"key":"10.1016\/j.tvjl.2004.11.004_bib3","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/0147-9571(92)90005-C","article-title":"Effects in calves of mixed infection with bovine viral diarrhoea virus and several other bovine viruses","volume":"15","author":"Castrucci","year":"1992","journal-title":"Comparative Immunology, Microbiology and Infectious Diseases"},{"key":"10.1016\/j.tvjl.2004.11.004_bib4","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0378-1135(96)01244-8","article-title":"The use of polymerase chain reaction assay for the detection of bovine herpesvirus 1 in semen during a natural outbreak of infectious bovine rhinotracheitis","volume":"53","author":"De Gee","year":"1996","journal-title":"Veterinary Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib5","first-page":"547","article-title":"Effect of storage conditions and culture technique on the isolation of infectious bovine rhinotracheitis virus from bovine semen","volume":"121","author":"Drew","year":"1987","journal-title":"Veterinary Record"},{"key":"10.1016\/j.tvjl.2004.11.004_bib6","doi-asserted-by":"crossref","first-page":"2498","DOI":"10.1128\/JCM.37.8.2498-2507.1999","article-title":"Detection of bovine herpesvirus type 1 in blood from naturally infected cattle by using a sensitive PCR that discriminates between wild-type virus and virus lacking glycoprotein E","volume":"37","author":"Fuchs","year":"1999","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib7","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/S0264-410X(96)00165-X","article-title":"A quantitative study of the efficacy of a deletion mutant bovine herpesvirus-1 differential vaccine in reducing the establishment of latency by wildtype virus","volume":"15","author":"Galeota","year":"1997","journal-title":"Vaccine"},{"key":"10.1016\/j.tvjl.2004.11.004_bib8","first-page":"317","article-title":"Bovine herpesviruses. I. Bovine herpesvirus 1","volume":"47","author":"Gibbs","year":"1977","journal-title":"Veterinary Bulletin"},{"key":"10.1016\/j.tvjl.2004.11.004_bib9","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1016\/0264-410X(95)98254-8","article-title":"An inactivated vaccine based on a glycoprotein E-negative strain of bovine herpesvirus 1 induces protective immunity and allows serological differentiation","volume":"13","author":"Kaashoek","year":"1995","journal-title":"Vaccine"},{"key":"10.1016\/j.tvjl.2004.11.004_bib10","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/0093-691X(80)90101-6","article-title":"The search for viruses in bovine semen, a review","volume":"14","author":"Kahrs","year":"1980","journal-title":"Theriogenology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib11","doi-asserted-by":"crossref","first-page":"1206","DOI":"10.2460\/ajvr.1994.55.09.1206","article-title":"Amplification of strains of bovine herpesvirus 1 by use of polymerase chain reaction with primers in the thymine kinase region","volume":"55","author":"Kibenge","year":"1994","journal-title":"American Journal of Veterinary Research"},{"key":"10.1016\/j.tvjl.2004.11.004_bib12","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0093-691X(86)90052-X","article-title":"Transmission of IBR\/IPV virus in bovine semen: a case report","volume":"25","author":"Kupferschmied","year":"1986","journal-title":"Theriogenology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib13","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0378-1135(95)00136-0","article-title":"The distinction of serologically related ruminant alphaherpesviruses by the polymerase chain reaction (PCR) and restriction endonuclease analysis","volume":"48","author":"Lyaku","year":"1996","journal-title":"Veterinary Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib14","first-page":"95","article-title":"The effects of IBR virus infection on reproductive function of cattle","volume":"1","author":"Miller","year":"1991","journal-title":"Veterinary Medicine"},{"key":"10.1016\/j.tvjl.2004.11.004_bib15","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/S0378-1135(00)00210-8","article-title":"A rapid and sensitive PCR-based diagnostic assay to detect bovine herpesvirus 1 in routine diagnostic submissions","volume":"75","author":"Moore","year":"2000","journal-title":"Veterinary Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib16","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/0147-9571(85)90052-9","article-title":"Diagnosis and prophylaxis of infectious bovine rhinotracheitis: the role of virus latency","volume":"8","author":"Pastoret","year":"1985","journal-title":"Comparative Immunology, Microbiology and Infectious Diseases"},{"key":"10.1016\/j.tvjl.2004.11.004_bib17","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/0378-1135(79)90050-6","article-title":"Effects of repeated treatment with dexamethasone with re-excretion pattern of infectious bovine rhinotracheitis virus and humoral response","volume":"4","author":"Pastoret","year":"1979","journal-title":"Veterinary Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib18","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/S0007-1935(05)80075-2","article-title":"The dangers of disease transmission by AI and embryo transfer","volume":"149","author":"Philpott","year":"1993","journal-title":"British Veterinary Journal"},{"key":"10.1016\/j.tvjl.2004.11.004_bib19","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/0378-1135(95)00169-7","article-title":"Rapid and high sensitivity test for direct detection of bovine herpesvirus-1 genome in clinical samples","volume":"49","author":"Santurde","year":"1996","journal-title":"Veterinary Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib20","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/0093-691X(82)90071-1","article-title":"A method to test large numbers of bovine semen samples for viral contamination and results of a study using this method","volume":"17","author":"Schultz","year":"1982","journal-title":"Theriogenology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib21","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0166-0934(99)00153-6","article-title":"Comparison of three polymerase chain reaction methods for routine detection of bovine herpesvirus 1 DNA in fresh bull semen","volume":"85","author":"Smits","year":"2000","journal-title":"Journal of Virological Methods"},{"key":"10.1016\/j.tvjl.2004.11.004_bib22","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1136\/vr.115.10.248","article-title":"Failure to demonstrate infectious bovine rhinotracheitis virus reactivation in parturient cows","volume":"8","author":"Thiry","year":"1984","journal-title":"Veterinary Record"},{"key":"10.1016\/j.tvjl.2004.11.004_bib23","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1128\/jcm.31.12.3129-3135.1993","article-title":"Development of a rapid and positive polymerase chain reaction assay for detection of bovine herpesvirus type 1 in bovine semen","volume":"31","author":"Van Engelenburg","year":"1993","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib24","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1128\/jcm.33.2.308-312.1995","article-title":"Excretion of bovine herpesvirus 1 in semen is detected much longer by PCR than by virus isolation","volume":"33","author":"Van Engelenburg","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib25","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1099\/0022-1317-76-9-2387","article-title":"A glycoprotein E deletion mutant of bovine herpesvirus 1 infects the same limited number of tissues in calves as wild type virus, but for a shorter period","volume":"76","author":"Van Engelenburg","year":"1995","journal-title":"Journal of General Virology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib26","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1136\/vr.132.2.32","article-title":"A subclinical infection of bulls with bovine herpesvirus type 1 at an AI centre","volume":"132","author":"Van Oirschot","year":"1993","journal-title":"Veterinary Record"},{"key":"10.1016\/j.tvjl.2004.11.004_bib27","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/0166-0934(93)90132-B","article-title":"Detection of bovine herpesvirus-1 (BHV-1) genome by PCR","volume":"41","author":"Vil\u010dek","year":"1993","journal-title":"Journal of Virological Methods"},{"key":"10.1016\/j.tvjl.2004.11.004_bib28","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/0378-1135(94)90077-9","article-title":"Rapid detection of the bovine herpesvirus-1 (BHV 1) using the polymerase chain reaction","volume":"42","author":"Vil\u010dek","year":"1994","journal-title":"Veterinary Microbiology"},{"key":"10.1016\/j.tvjl.2004.11.004_bib29","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1007\/BF00366546","article-title":"A polymerase chain reaction (PCR) assay for the detection of bovine herpesvirus 1 (BHV1) in selectively digested whole bovine semen","volume":"20","author":"Wagter","year":"1996","journal-title":"Veterinary Research Communication"},{"key":"10.1016\/j.tvjl.2004.11.004_bib30","first-page":"506","article-title":"Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material","volume":"10","author":"Walsh","year":"1991","journal-title":"BioTechniques"},{"key":"10.1016\/j.tvjl.2004.11.004_bib31","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1177\/104063879200400321","article-title":"Isolation of bovine herpesvirus 1 from preputial swabs and semen of bulls with balanoposthitis","volume":"4","author":"Weiblen","year":"1992","journal-title":"Journal of Veterinary Diagnostic Investigation"},{"key":"10.1016\/j.tvjl.2004.11.004_bib32","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/0166-0934(93)90015-J","article-title":"Detection of bovine herpesvirus-1 in bovine semen by a nested PCR assay","volume":"44","author":"Wiedmann","year":"1993","journal-title":"Journal of Virological Methods"},{"key":"10.1016\/j.tvjl.2004.11.004_bib33","series-title":"Herpesvirus Diseases of Cattle, Horses and Pigs","first-page":"1","article-title":"Infectious bovine rhinotracheitis\/vulvovaginitis (BHV1)","author":"Wyler","year":"1989"},{"key":"10.1016\/j.tvjl.2004.11.004_bib34","first-page":"102","article-title":"Comparison of dot blot hybridisation, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (BHV-1) in artificially infected bovine semen","volume":"59","author":"Xia","year":"1995","journal-title":"Canadian Journal of Veterinary Research"}],"container-title":["The Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1090023304002461?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1090023304002461?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T18:24:39Z","timestamp":1761071079000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1090023304002461"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5]]},"references-count":34,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["S1090023304002461"],"URL":"https:\/\/doi.org\/10.1016\/j.tvjl.2004.11.004","relation":{},"ISSN":["1090-0233"],"issn-type":[{"value":"1090-0233","type":"print"}],"subject":[],"published":{"date-parts":[[2006,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular detection of BHV-1 in artificially inoculated semen and in the semen of a latently infected bull treated with dexamethasone","name":"articletitle","label":"Article Title"},{"value":"The Veterinary Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tvjl.2004.11.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2004 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}