{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:33:28Z","timestamp":1785760408964,"version":"3.56.0"},"reference-count":41,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1800505"],"award-info":[{"award-number":["2021YFD1800505"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012431","name":"Jiangsu Agricultural Science and Technology Independent Innovation Fund","doi-asserted-by":"publisher","award":["CX(23)1029"],"award-info":[{"award-number":["CX(23)1029"]}],"id":[{"id":"10.13039\/501100012431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007540","name":"Jiangsu Agricultural Science and Technology Innovation Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007540","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Veterinary Journal"],"published-print":{"date-parts":[[2026,2]]},"DOI":"10.1016\/j.tvjl.2026.106567","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:42:21Z","timestamp":1768520541000},"page":"106567","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Development and preliminary validation of a rapid on-site detection method for Schmallenberg virus using RT-RAA-LFD"],"prefix":"10.1016","volume":"315","author":[{"given":"Yiran","family":"Zhao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruojun","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huixing","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaojian","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjie","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanke","family":"Shan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.tvjl.2026.106567_bib1","doi-asserted-by":"crossref","first-page":"1883","DOI":"10.1128\/JCM.00167-14","article-title":"Rapid genome detection of Schmallenberg virus and bovine viral diarrhea virus by use of isothermal amplification methods and high-speed real-time reverse transcriptase PCR","volume":"52","author":"Aebischer","year":"2014","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1016\/j.tvjl.2026.106567_bib2","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1016\/j.prevetmed.2014.02.012","article-title":"The Schmallenberg virus epidemic in Europe-2011-2013","volume":"116","author":"Afonso","year":"2014","journal-title":"Preventive Veterinary Medicine"},{"key":"10.1016\/j.tvjl.2026.106567_bib3","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.prevetmed.2014.09.007","article-title":"Schmallenberg virus","volume":"116","author":"Afonso","year":"2014","journal-title":"Preventive Veterinary Medicine"},{"key":"10.1016\/j.tvjl.2026.106567_bib4","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1016\/j.prevetmed.2014.06.018","article-title":"Schmallenberg virus: lessons learned","volume":"116","author":"Angot","year":"2014","journal-title":"Preventive Veterinary Medicine"},{"key":"10.1016\/j.tvjl.2026.106567_bib5","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1177\/1040638720947199","article-title":"Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus","volume":"32","author":"Azkur","year":"2020","journal-title":"Journal of Veterinary Diagnostic Investigation"},{"key":"10.1016\/j.tvjl.2026.106567_bib6","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S0950268812002245","article-title":"Schmallenberg virus\u2014a novel orthobunyavirus emerging in Europe","volume":"141","author":"Beer","year":"2013","journal-title":"Epidemiology and Infection"},{"key":"10.1016\/j.tvjl.2026.106567_bib7","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0053446","article-title":"Validation of a commercially available indirect ELISA using a nucleocapsid recombinant protein for detection of Schmallenberg virus antibodies","volume":"8","author":"Br\u00e9ard","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.tvjl.2026.106567_bib8","doi-asserted-by":"crossref","DOI":"10.1016\/j.jviromet.2019.113685","article-title":"Development and analytical validation of a group-specific RT-qPCR assay for the detection of the Simbu serogroup orthobunyaviruses","volume":"271","author":"Camar\u00e3o","year":"2019","journal-title":"Journal of Virological Methods"},{"key":"10.1016\/j.tvjl.2026.106567_bib9","first-page":"261","article-title":"Schmallenberg virus infection of ruminants: challenges and opportunities for veterinarians","volume":"6","author":"Claine","year":"2015","journal-title":"Veterinary Medicine"},{"key":"10.1016\/j.tvjl.2026.106567_bib10","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.mcp.2014.11.005","article-title":"Influence of sequence mismatches on the specificity of recombinase polymerase amplification technology","volume":"29","author":"Daher","year":"2015","journal-title":"Molecular and Cellular Probes"},{"key":"10.1016\/j.tvjl.2026.106567_bib11","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.3390\/v11111065","article-title":"Schmallenberg disease\u2014a newly emerged Culicoides-borne viral disease of ruminants","volume":"11","author":"Endalew","year":"2019","journal-title":"Viruses"},{"key":"10.1016\/j.tvjl.2026.106567_bib12","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2023.133531","article-title":"A fully-enclosed prototype 'pen' for rapid detection of SARS-CoV-2 based on RT-RPA with dipstick assay at point-of-care testing","volume":"383","author":"Fu","year":"2023","journal-title":"Sensors and Actuators B: Chemical"},{"key":"10.1016\/j.tvjl.2026.106567_bib13","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.ijbiomac.2022.10.153","article-title":"Development and application of a recombinase-aided amplification and lateral flow assay for rapid detection of pseudorabies virus from clinical crude samples","volume":"224","author":"Guo","year":"2023","journal-title":"International Journal of Biological Macromolecules"},{"key":"10.1016\/j.tvjl.2026.106567_bib14","doi-asserted-by":"crossref","first-page":"469","DOI":"10.3201\/eid1803.111905","article-title":"Novel orthobunyavirus in cattle, Europe, 2011","volume":"18","author":"Hoffmann","year":"2012","journal-title":"Emerging Infectious Diseases"},{"key":"10.1016\/j.tvjl.2026.106567_bib15","doi-asserted-by":"crossref","DOI":"10.1016\/j.snb.2025.137612","article-title":"A portable all-in-one microfluidic platform integrated with CRISPR-based extraction-free assay for rapid and on-site detection of monkeypox and lumpy skin disease","volume":"436","author":"Huang","year":"2025","journal-title":"Sensors and Actuators B: Chemical"},{"key":"10.1016\/j.tvjl.2026.106567_bib16","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1016\/j.tvjl.2013.08.013","article-title":"First detection of Schmallenberg virus in elk (Alces alces) indicating infection of wildlife in Bia\u0142owie\u017ca National Park in Poland","volume":"198","author":"Larska","year":"2013","journal-title":"The Veterinary Journal"},{"key":"10.1016\/j.tvjl.2026.106567_bib17","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1186\/s12917-014-0307-3","article-title":"Cross-sectional study of Schmallenberg virus seroprevalence in wild ruminants in Poland at the end of the vector season of 2013","volume":"10","author":"Larska","year":"2014","journal-title":"BMC Veterinary Research"},{"key":"10.1016\/j.tvjl.2026.106567_bib18","article-title":"Advances in the application of recombinase-aided amplification combined with CRISPR-Cas technology in quick detection of pathogenic microbes","volume":"11","author":"Li","year":"2023","journal-title":"Frontiers in Bioengineering and Biotechnology"},{"key":"10.1016\/j.tvjl.2026.106567_bib19","doi-asserted-by":"crossref","DOI":"10.3389\/fcimb.2022.922146","article-title":"Rapid visual detection of Plasmodium using recombinase-aided amplification with lateral flow dipstick assay","volume":"12","author":"Lin","year":"2022","journal-title":"Frontiers in Cellular and Infection Microbiology"},{"key":"10.1016\/j.tvjl.2026.106567_bib20","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1080\/14737159.2022.2109964","article-title":"Critical insight into recombinase polymerase amplification technology","volume":"22","author":"Munawar","year":"2022","journal-title":"Expert Review of Molecular Diagnostics"},{"key":"10.1016\/j.tvjl.2026.106567_bib21","first-page":"112","article-title":"Diarrhea and loss of production on Dutch dairy farms caused by the Schmallenberg virus","volume":"137","author":"Muskens","year":"2012","journal-title":"Tijdschrift voor Diergeneeskunde"},{"key":"10.1016\/j.tvjl.2026.106567_bib22","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s13028-014-0071-1","article-title":"Development and evaluation of an indirect enzyme-linked immunosorbent assay for serological detection of Schmallenberg virus antibodies in ruminants using whole virus antigen","volume":"56","author":"N\u00e4slund","year":"2014","journal-title":"Acta Veterinaria Scandinavica"},{"key":"10.1016\/j.tvjl.2026.106567_bib23","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1177\/0300985814560231","article-title":"Ovine and bovine congenital abnormalities associated with intrauterine infection with Schmallenberg virus","volume":"52","author":"Peperkamp","year":"2015","journal-title":"Veterinary Pathology"},{"key":"10.1016\/j.tvjl.2026.106567_bib24","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pbio.0040204","article-title":"DNA detection using recombination proteins","volume":"4","author":"Piepenburg","year":"2006","journal-title":"PLoS Biology"},{"key":"10.1016\/j.tvjl.2026.106567_bib25","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.jmoldx.2014.01.001","article-title":"Getting things backwards to prevent primer dimers","volume":"16","author":"Poritz","year":"2014","journal-title":"The Journal of Molecular Diagnostics"},{"key":"10.1016\/j.tvjl.2026.106567_bib26","doi-asserted-by":"crossref","first-page":"1204","DOI":"10.3201\/eid1807.120385","article-title":"Culicoids as vectors of Schmallenberg virus","volume":"18","author":"Rasmussen","year":"2012","journal-title":"Emerging Infectious Diseases"},{"key":"10.1016\/j.tvjl.2026.106567_bib27","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.3201\/eid1811.120650","article-title":"Lack of evidence for zoonotic transmission of Schmallenberg virus","volume":"18","author":"Reusken","year":"2012","journal-title":"Emerging Infectious Diseases"},{"key":"10.1016\/j.tvjl.2026.106567_bib28","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1186\/s12879-019-3814-9","article-title":"A rapid and sensitive recombinase-aided amplification assay to detect hepatitis B virus without DNA extraction","volume":"19","author":"Shen","year":"2019","journal-title":"BMC Infectious Diseases"},{"key":"10.1016\/j.tvjl.2026.106567_bib29","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117178","article-title":"Lift-CM: An integrated lift-heater centrifugal microfluidic platform for point-of-care pathogen nucleic acid detection using isothermal amplification and CRISPR\/Cas12a","volume":"274","author":"Shu","year":"2025","journal-title":"Biosensors and Bioelectronics"},{"key":"10.1016\/j.tvjl.2026.106567_bib30","doi-asserted-by":"crossref","first-page":"3361","DOI":"10.1038\/srep03361","article-title":"The influence of the wind in the Schmallenberg virus outbreak in Europe","volume":"3","author":"Sedda","year":"2013","journal-title":"Scientific Reports"},{"key":"10.1016\/j.tvjl.2026.106567_bib31","doi-asserted-by":"crossref","first-page":"2266","DOI":"10.1111\/tbed.14241","article-title":"Detection of pseudorabies virus with a real-time recombinase-aided amplification assay","volume":"69","author":"Tu","year":"2022","journal-title":"Transboundary Emerging Diseases"},{"key":"10.1016\/j.tvjl.2026.106567_bib32","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.rvsc.2013.04.022","article-title":"Development and validation of an indirect enzyme-linked immunosorbent assay for the detection of antibodies against Schmallenberg virus in blood samples from ruminants","volume":"95","author":"van der Heijden","year":"2013","journal-title":"Research in Veterinary Science"},{"key":"10.1016\/j.tvjl.2026.106567_bib33","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117607","article-title":"Exploring a CRISPR\/Cas12a-powered impedimetric biosensor for amplification-free detection of a pathogenic bacterial DNA","volume":"285","author":"Wachholz Junior","year":"2025","journal-title":"Biosensors and Bioelectronics"},{"key":"10.1016\/j.tvjl.2026.106567_bib34","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2025.117679","article-title":"A time-resolved luminescence assay using time-to-space conversion strategy for the sensitive detection of influenza virus on a microfluidic chip","volume":"287","author":"Wang","year":"2025","journal-title":"Biosensors and Bioelectronics"},{"key":"10.1016\/j.tvjl.2026.106567_bib35","doi-asserted-by":"crossref","first-page":"287","DOI":"10.3390\/vaccines8020287","article-title":"Schmallenberg virus: To vaccinate, or not to vaccinate?","volume":"8","author":"Wernike","year":"2020","journal-title":"Vaccines"},{"key":"10.1016\/j.tvjl.2026.106567_bib36","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.3389\/fmicb.2020.01015","article-title":"A recombinase polymerase amplification and lateral flow strip combined method that detects Salmonella enterica serotype Typhimurium with no worry of primer-dependent artifacts","volume":"11","author":"Wu","year":"2020","journal-title":"Frontiers in Microbiology"},{"key":"10.1016\/j.tvjl.2026.106567_bib37","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.ijid.2020.03.075","article-title":"Rapid visual detection of dengue virus by combining reverse transcription recombinase-aided amplification with lateral-flow dipstick assay","volume":"95","author":"Xiong","year":"2020","journal-title":"International Journal of Infectious Diseases"},{"key":"10.1016\/j.tvjl.2026.106567_bib38","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1039\/C3MB70304E","article-title":"Isothermal amplified detection of DNA and RNA","volume":"10","author":"Yan","year":"2014","journal-title":"Molecular BioSystems"},{"key":"10.1016\/j.tvjl.2026.106567_bib39","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1080\/21505594.2022.2116157","article-title":"A nucleic acid detection assay combining reverse transcription recombinase-aided amplification with a lateral flow dipstick for the rapid visual detection of porcine deltacoronavirus","volume":"13","author":"Zeng","year":"2022","journal-title":"Virulence"},{"key":"10.1016\/j.tvjl.2026.106567_bib40","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1007\/s11250-017-1433-2","article-title":"Preliminary serological evidence for Schmallenberg virus infection in China","volume":"50","author":"Zhai","year":"2018","journal-title":"Tropical Animal Health and Production"},{"key":"10.1016\/j.tvjl.2026.106567_bib41","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1016\/j.mimet.2017.06.011","article-title":"Rapid detection of Salmonella with recombinase-aided amplification","volume":"139","author":"Zhang","year":"2017","journal-title":"Journal of Microbiological Methods"}],"container-title":["The Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1090023326000237?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1090023326000237?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T02:30:11Z","timestamp":1773973811000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1090023326000237"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2]]},"references-count":41,"alternative-id":["S1090023326000237"],"URL":"https:\/\/doi.org\/10.1016\/j.tvjl.2026.106567","relation":{},"ISSN":["1090-0233"],"issn-type":[{"value":"1090-0233","type":"print"}],"subject":[],"published":{"date-parts":[[2026,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Development and preliminary validation of a rapid on-site detection method for Schmallenberg virus using RT-RAA-LFD","name":"articletitle","label":"Article Title"},{"value":"The Veterinary Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tvjl.2026.106567","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"106567"}}