{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T04:29:06Z","timestamp":1793680146062,"version":"4.5.1"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-012"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-004"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["The Veterinary Journal"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.tvjl.2026.106950","type":"journal-article","created":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:11:22Z","timestamp":1792797082000},"page":"106950","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["A novel PRRSV-2 M antibody-blocking ELISA shows superior sensitivity over N antibody assays for evaluating maternal antibodies"],"prefix":"10.1016","volume":"320","author":[{"given":"Zhenyang","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixuan","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueli","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyu","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahao","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongliang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinmei","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongqing","family":"An","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.tvjl.2026.106950_bib1","doi-asserted-by":"crossref","first-page":"7811","DOI":"10.1038\/s41598-018-26036-w","article-title":"Genetic diversity of PRRSV 1 in Central Eastern Europe in 1994-2014: origin and evolution of the virus in the region","volume":"8","author":"Balka","year":"2018","journal-title":"Sci. Rep."},{"issue":"2","key":"10.1016\/j.tvjl.2026.106950_bib2","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1177\/104063879200400202","article-title":"Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332)","volume":"4","author":"Benfield","year":"1992","journal-title":"J. Vet. Diagn. Invest."},{"issue":"2","key":"10.1016\/j.tvjl.2026.106950_bib3","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1177\/104063879200400201","article-title":"Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs","volume":"4","author":"Collins","year":"1992","journal-title":"J. Vet. Diagn. Invest."},{"issue":"2","key":"10.1016\/j.tvjl.2026.106950_bib4","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1016\/j.virol.2006.05.036","article-title":"Serologic marker candidates identified among B-cell linear epitopes of Nsp2 and structural proteins of a North American strain of porcine reproductive and respiratory syndrome virus","volume":"353","author":"de Lima","year":"2006","journal-title":"Virology"},{"issue":"11","key":"10.1016\/j.tvjl.2026.106950_bib5","doi-asserted-by":"crossref","DOI":"10.3390\/jof8111179","article-title":"Non-mammalian eukaryotic expression systems yeast and fungi in the production of biologics","volume":"8","author":"Garvey","year":"2022","journal-title":"J. Fungi"},{"key":"10.1016\/j.tvjl.2026.106950_bib6","doi-asserted-by":"crossref","DOI":"10.1016\/j.tvjl.2025.106482","article-title":"Novel real-time quantitative RT-PCR for detection of PRRSV-1 and PRRSV-2 strains circulating in China: a study based on whole-genome characteristics and epidemiological features","volume":"314","author":"Gong","year":"2025","journal-title":"Vet. J."},{"key":"10.1016\/j.tvjl.2026.106950_bib7","first-page":"1","article-title":"Isolation and identification of Procine reproductive and respiratory syndrome virus from aborted fetuses suspected of PRRS","volume":"87","author":"Guo","year":"1996","journal-title":"Chin. J. Anim. Poult. Infect. Dis."},{"issue":"Pt 1","key":"10.1016\/j.tvjl.2026.106950_bib8","article-title":"Improved detection sensitivity of anti-PRV variant antibodies through preparation of anti-gB and anti-gE monoclonal antibodies and development of blocking ELISAs","volume":"260","author":"Guo","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"2","key":"10.1016\/j.tvjl.2026.106950_bib9","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1007\/s11262-008-0322-1","article-title":"Molecular mutations associated with the in vitro passage of virulent porcine reproductive and respiratory syndrome virus","volume":"38","author":"Han","year":"2009","journal-title":"Virus Genes."},{"issue":"1-2","key":"10.1016\/j.tvjl.2026.106950_bib10","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.vetmic.2011.12.012","article-title":"Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation","volume":"157","author":"Leng","year":"2012","journal-title":"Vet. Microbiol."},{"issue":"2","key":"10.1016\/j.tvjl.2026.106950_bib11","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.02350-24","article-title":"Engineering a live-attenuated porcine reproductive and respiratory syndrome virus vaccine to prevent RNA recombination by rewiring transcriptional regulatory sequences","volume":"16","author":"Li","year":"2025","journal-title":"mBio"},{"issue":"Pt 2","key":"10.1016\/j.tvjl.2026.106950_bib12","article-title":"Development and application of a blocking ELISA based on a N protein monoclonal antibody for the antibody detection against porcine reproductive and respiratory syndrome virus 2","volume":"269","author":"Li","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"3","key":"10.1016\/j.tvjl.2026.106950_bib13","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/CVI.00304-06","article-title":"Protection against porcine reproductive and respiratory syndrome virus (PRRSV) infection through passive transfer of PRRSV-neutralizing antibodies is dose dependent","volume":"14","author":"Lopez","year":"2007","journal-title":"Clin. Vaccin. Immunol."},{"issue":"3","key":"10.1016\/j.tvjl.2026.106950_bib14","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/j.vetimm.2004.09.005","article-title":"Role of neutralizing antibodies in PRRSV protective immunity","volume":"102","author":"Lopez","year":"2004","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"1-2","key":"10.1016\/j.tvjl.2026.106950_bib15","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetimm.2015.07.003","article-title":"Innate and adaptive immunity against Porcine Reproductive and Respiratory Syndrome Virus","volume":"167","author":"Loving","year":"2015","journal-title":"Vet. Immunol. Immunopathol."},{"issue":"2-4","key":"10.1016\/j.tvjl.2026.106950_bib16","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.vetmic.2014.11.016","article-title":"Re-emerging of porcine respiratory and reproductive syndrome virus (lineage 3) and increased pathogenicity after genomic recombination with vaccine variant","volume":"175","author":"Lu","year":"2015","journal-title":"Vet. Microbiol."},{"key":"10.1016\/j.tvjl.2026.106950_bib17","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1146\/annurev-animal-022114-111025","article-title":"Porcine Reproductive and Respiratory Syndrome Virus (PRRSV): Pathogenesis and Interaction with the Immune System","volume":"4","author":"Lunney","year":"2016","journal-title":"Annu. Rev. Anim. Biosci."},{"issue":"5","key":"10.1016\/j.tvjl.2026.106950_bib18","article-title":"Research Progress on Glycoprotein 5 of Porcine Reproductive and Respiratory Syndrome Virus","volume":"13","author":"Luo","year":"2023","journal-title":"Anim. (Basel)"},{"key":"10.1016\/j.tvjl.2026.106950_bib19","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2025.110534","article-title":"The mRNA vaccine expressing fused structural protein of PRRSV protects piglets against PRRSV challenge","volume":"305","author":"Mou","year":"2025","journal-title":"Vet. Microbiol."},{"issue":"1","key":"10.1016\/j.tvjl.2026.106950_bib20","first-page":"1","article-title":"Pseudorabies virus infections in pigs. Role of viral proteins in virulence, pathogenesis and transmission","volume":"28","author":"Mulder","year":"1997","journal-title":"Vet. Res."},{"issue":"1-2","key":"10.1016\/j.tvjl.2026.106950_bib21","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.virusres.2010.08.014","article-title":"Molecular epidemiology of PRRSV: a phylogenetic perspective","volume":"154","author":"Shi","year":"2010","journal-title":"Virus Res."},{"issue":"17","key":"10.1016\/j.tvjl.2026.106950_bib22","doi-asserted-by":"crossref","first-page":"8700","DOI":"10.1128\/JVI.02551-09","article-title":"Phylogeny-based evolutionary, demographical, and geographical dissection of North American type 2 porcine reproductive and respiratory syndrome viruses","volume":"84","author":"Shi","year":"2010","journal-title":"J. Virol."},{"issue":"1-2","key":"10.1016\/j.tvjl.2026.106950_bib23","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.vetmic.2013.02.029","article-title":"Molecular evolution of PRRSV in Europe: current state of play","volume":"165","author":"Stadejek","year":"2013","journal-title":"Vet. Microbiol."},{"issue":"3","key":"10.1016\/j.tvjl.2026.106950_bib24","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1080\/01652176.1991.9694297","article-title":"Experimental reproduction of porcine epidemic abortion and respiratory syndrome (mystery swine disease) by infection with Lelystad virus: Koch's postulates fulfilled","volume":"13","author":"Terpstra","year":"1991","journal-title":"Vet. Q"},{"key":"10.1016\/j.tvjl.2026.106950_bib25","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2174\/1874357901711010059","article-title":"NADC30-Like porcine reproductive and respiratory syndrome in China","volume":"11","author":"Tian","year":"2017","journal-title":"Open. Virol. J."},{"issue":"9","key":"10.1016\/j.tvjl.2026.106950_bib26","doi-asserted-by":"crossref","first-page":"1434","DOI":"10.3201\/eid1309.070399","article-title":"Highly pathogenic porcine reproductive and respiratory syndrome, China","volume":"13","author":"Tong","year":"2007","journal-title":"Emerg. Infect. Dis."},{"issue":"12","key":"10.1016\/j.tvjl.2026.106950_bib27","doi-asserted-by":"crossref","first-page":"6515","DOI":"10.1128\/JVI.03287-14","article-title":"A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity","volume":"89","author":"Trible","year":"2015","journal-title":"J. Virol."},{"key":"10.1016\/j.tvjl.2026.106950_bib28","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.virusres.2014.09.010","article-title":"Membrane proteins of arterivirus particles: structure, topology, processing and function","volume":"194","author":"Veit","year":"2014","journal-title":"Virus Res."},{"issue":"9","key":"10.1016\/j.tvjl.2026.106950_bib29","doi-asserted-by":"crossref","first-page":"2633","DOI":"10.1007\/s00705-021-05156-1","article-title":"Changes to virus taxonomy and to the International Code of Virus Classification and Nomenclature ratified by the International Committee on Taxonomy of Viruses (2021)","volume":"166","author":"Walker","year":"2021","journal-title":"Arch. Virol."},{"issue":"1","key":"10.1016\/j.tvjl.2026.106950_bib30","doi-asserted-by":"crossref","DOI":"10.1155\/tbed\/7831655","article-title":"Development and application of a blocking ELISA for the detection of feline calicivirus antibodies based on monoclonal antibodies against VP1 protein","volume":"2026","author":"Wang","year":"2026","journal-title":"Transbound. Emerg. Dis."},{"issue":"11","key":"10.1016\/j.tvjl.2026.106950_bib31","doi-asserted-by":"crossref","first-page":"3021","DOI":"10.1007\/s00705-014-2174-4","article-title":"Characterisation of novel linear antigen epitopes on North American-type porcine reproductive and respiratory syndrome virus M protein","volume":"159","author":"Wang","year":"2014","journal-title":"Arch. Virol."},{"issue":"5","key":"10.1016\/j.tvjl.2026.106950_bib32","doi-asserted-by":"crossref","DOI":"10.1111\/tbed.14485","article-title":"Novel characteristics of Chinese NADC34-like PRRSV during 2020-2021","volume":"69","author":"Xu","year":"2022","journal-title":"Transbound. Emerg. Dis."},{"issue":"3","key":"10.1016\/j.tvjl.2026.106950_bib33","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0033756","article-title":"Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in southwest China from 2007 to 2009","volume":"7","author":"Yin","year":"2012","journal-title":"PLoS. One"},{"issue":"3","key":"10.1016\/j.tvjl.2026.106950_bib34","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1177\/104063879400600326","article-title":"A modified serum neutralization test for the detection of antibody to porcine reproductive and respiratory syndrome virus in swine sera","volume":"6","author":"Yoon","year":"1994","journal-title":"J. Vet. Diagn. Invest."},{"issue":"6","key":"10.1016\/j.tvjl.2026.106950_bib35","doi-asserted-by":"crossref","DOI":"10.1128\/spectrum.01984-23","article-title":"Protective efficacy of a candidate live attenuated vaccine derived from the SD-R strain of lineage 1 porcine reproductive and respiratory syndrome virus against a lethal challenge with HP-PRRSV HuN4 in piglets","volume":"11","author":"Zhang","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"10.1016\/j.tvjl.2026.106950_bib36","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.vetmic.2018.06.017","article-title":"Emergence of novel porcine reproductive and respiratory syndrome viruses (ORF5 RFLP 1-7-4 viruses) in China","volume":"222","author":"Zhang","year":"2018","journal-title":"Vet. Microbiol."},{"issue":"Pt 3","key":"10.1016\/j.tvjl.2026.106950_bib37","article-title":"Intervention strategies targeting virus and host factors against porcine reproductive and respiratory syndrome virus: a systematic review","volume":"279","author":"Zhang","year":"2024","journal-title":"Int. J. Biol. Macromol."},{"issue":"20","key":"10.1016\/j.tvjl.2026.106950_bib38","doi-asserted-by":"crossref","first-page":"10712","DOI":"10.1128\/JVI.01446-15","article-title":"Importation and recombination are responsible for the latest emergence of highly pathogenic porcine reproductive and respiratory syndrome virus in China","volume":"89","author":"Zhao","year":"2015","journal-title":"J. Virol."},{"issue":"10","key":"10.1016\/j.tvjl.2026.106950_bib39","doi-asserted-by":"crossref","DOI":"10.3390\/v10100551","article-title":"Genetic characterization and pathogenicity of a novel recombined porcine reproductive and respiratory syndrome virus 2 among Nadc30-Like, Jxa1-Like, and Mlv-Like Strains","volume":"10","author":"Zhou","year":"2018","journal-title":"Viruses"}],"container-title":["The Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1090023326004077?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S1090023326004077?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T04:23:33Z","timestamp":1793679813000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S1090023326004077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":39,"alternative-id":["S1090023326004077"],"URL":"https:\/\/doi.org\/10.1016\/j.tvjl.2026.106950","relation":{},"ISSN":["1090-0233"],"issn-type":[{"value":"1090-0233","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A novel PRRSV-2 M antibody-blocking ELISA shows superior sensitivity over N antibody assays for evaluating maternal antibodies","name":"articletitle","label":"Article Title"},{"value":"The Veterinary Journal","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.tvjl.2026.106950","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Elsevier Ltd. All rights are reserved, including those for text and data mining, AI training, and similar technologies.","name":"copyright","label":"Copyright"}],"article-number":"106950"}}