{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:56:36Z","timestamp":1790301396760,"version":"4.1.0"},"reference-count":43,"publisher":"Elsevier BV","issue":"40","license":[{"start":{"date-parts":[[2005,9,1]],"date-time":"2005-09-01T00:00:00Z","timestamp":1125532800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2005,9]]},"DOI":"10.1016\/j.vaccine.2005.05.013","type":"journal-article","created":{"date-parts":[[2005,6,10]],"date-time":"2005-06-10T15:55:01Z","timestamp":1118418901000},"page":"4811-4820","source":"Crossref","is-referenced-by-count":27,"title":["Induction of protective immunity by Legionella pneumophila flagellum in an A\/J mouse model"],"prefix":"10.1016","volume":"23","author":[{"given":"Maria Luisa","family":"Ricci","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Torosantucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Scaturro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Chiani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucilla","family":"Baldassarri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maddalena Castellani","family":"Pastoris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2005.05.013_bib1","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1056\/NEJM197712012972201","article-title":"Legionnaires\u2019 disease. Description of an epidemic of pneumonia","volume":"297","author":"Fraser","year":"1977","journal-title":"New Engl J Med"},{"key":"10.1016\/j.vaccine.2005.05.013_bib2","series-title":"Opportunistic intracellular bacteria and immunity","first-page":"131","article-title":"Pathogenesis of Legionella pneumophila infection","author":"Hoffman","year":"1999"},{"key":"10.1016\/j.vaccine.2005.05.013_bib3","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1172\/JCI109874","article-title":"Silverstein SC Legionnaires\u2019 disease bacterium (Legionella pneumophila) multiplies intracellularly in human monocytes","volume":"66","author":"Horwitz","year":"1980","journal-title":"J Clin Invest"},{"key":"10.1016\/j.vaccine.2005.05.013_bib4","series-title":"Opportunistic Intracellular Bacteria and Immunity","first-page":"149","article-title":"Immune responses to Legionella","author":"Klein","year":"1999"},{"key":"10.1016\/j.vaccine.2005.05.013_bib5","doi-asserted-by":"crossref","first-page":"3454","DOI":"10.1128\/IAI.62.8.3454-3462.1994","article-title":"Human and guinea pig immune responses to Legionella pneumophila protein antigens OmpS and Hsp60","volume":"62","author":"Weeratna","year":"1994","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib6","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1084\/jem.169.3.691","article-title":"Vaccination with the major secretory protein of Legionella pneumophila induces cell-mediated and protective immunity in a guinea pig model of Legionnaires\u2019 disease","volume":"169","author":"Blander","year":"1989","journal-title":"J Exp Med"},{"key":"10.1016\/j.vaccine.2005.05.013_bib7","first-page":"285","article-title":"Vaccination with the major secretory protein of Legionella induces humoral and cell-mediated immune responses and protective immunity across different serogroups of Legionella pneumophila and different species of Legionella","volume":"147","author":"Blander","year":"1991","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib8","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1172\/JCI116253","article-title":"Major cytoplasmic membrane protein of Legionella pneumophila, a genus common antigen and member of the hsp 60 family of heat shock proteins, induces protective immunity in a guinea pig model of Legionnaires\u2019 disease","volume":"91","author":"Blander","year":"1993","journal-title":"J Clin Invest"},{"key":"10.1016\/j.vaccine.2005.05.013_bib9","doi-asserted-by":"crossref","first-page":"685","DOI":"10.1128\/IAI.45.3.685-691.1984","article-title":"Vaccination against Legionella pneumophila: serum antibody correlates with protection induced by heat-killed or acetone-killed cells against intraperitoneal but not aerosol infection in guinea pigs","volume":"45","author":"Eisenstein","year":"1984","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib10","doi-asserted-by":"crossref","first-page":"1099","DOI":"10.1038\/35074106","article-title":"The innate immune response to bacterial flagellin is mediated by toll-like receptor 5","volume":"410","author":"Hayashi","year":"2001","journal-title":"Nature (London)"},{"key":"10.1016\/j.vaccine.2005.05.013_bib11","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1017\/S0022172400062252","article-title":"A comparison of virulence of two strains of Legionella pneumophila based on experimental aerosol infection of guinea pigs","volume":"95","author":"Jepras","year":"1985","journal-title":"J Hyg"},{"key":"10.1016\/j.vaccine.2005.05.013_bib12","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/S0065-2911(08)60007-7","article-title":"The bacterial flagellum and flagellar motor: structure, assembly, and function","volume":"32","author":"Jones","year":"1991","journal-title":"Adv Microb Physiol"},{"key":"10.1016\/j.vaccine.2005.05.013_bib13","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1128\/IAI.70.1.192-198.2002","article-title":"Comparison of inflammation, organ damage, and oxidant stress induced by Salmonella enterica serovar Muenchen flagellin and serovar Enteritidis lipopolysaccharide","volume":"70","author":"Liaudet","year":"2002","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib14","doi-asserted-by":"crossref","first-page":"5176","DOI":"10.1128\/IAI.67.10.5176-5185.1999","article-title":"Induction of cytokine synthesis by flagella from gram-negative bacteria may be dependent on the activation or differentiation state of human monocytes","volume":"67","author":"Ciacci-Woolwine","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib15","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1128\/IAI.66.7.3029-3034.1998","article-title":"Expression of Legionella pneumophila virulence traits in response to growth conditions","volume":"66","author":"Byrne","year":"1998","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib16","doi-asserted-by":"crossref","first-page":"715","DOI":"10.7326\/0003-4819-93-5-715","article-title":"Flagella on Legionnaires\u2019 disease bacteria in the human lung","volume":"93","author":"Chandler","year":"1980","journal-title":"Ann Intern Med"},{"key":"10.1016\/j.vaccine.2005.05.013_bib17","doi-asserted-by":"crossref","first-page":"2116","DOI":"10.1128\/IAI.69.4.2116-2122.2001","article-title":"Flagellum of Legionella pneumophila positively affects the early phase of infection of eukaryotic host cells","volume":"69","author":"Dietrich","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib18","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1078\/1438-4221-00259","article-title":"The flagellum of Legionella pneumophila and its link to the expression of the virulent phenotype","volume":"293","author":"Heuner","year":"2003","journal-title":"Int J Med Microbiol"},{"key":"10.1016\/j.vaccine.2005.05.013_bib19","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1006\/mpat.1997.0134","article-title":"Flagella are positive predictor virulence in Legionella","volume":"23","author":"Bosshardt","year":"1997","journal-title":"Microb Pathog"},{"key":"10.1016\/j.vaccine.2005.05.013_bib20","doi-asserted-by":"crossref","first-page":"4738","DOI":"10.1128\/IAI.65.11.4738-4746.1997","article-title":"Utilization of similar mechanisms by Legionella pneumophila to parasitize two evolutionarily distant host cells, mammalian macrophages and protozoa","volume":"65","author":"Gao","year":"1997","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib21","series-title":"Legionella, 2002","first-page":"43","article-title":"Function and expression of Legionella pneumophila surface factors","author":"Heuner","year":"2002"},{"key":"10.1016\/j.vaccine.2005.05.013_bib22","doi-asserted-by":"crossref","first-page":"4928","DOI":"10.1128\/IAI.63.12.4928-4932.1995","article-title":"Association of flagellum expression and intracellular growth of Legionella pneumophila","volume":"63","author":"Pruckler","year":"1995","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib23","series-title":"Legionella, 2002","first-page":"129","article-title":"Protection against lethal challenge by Legionella pneumophila in A\/J mice following immunisation with flagella","author":"Ricci","year":"2002"},{"key":"10.1016\/j.vaccine.2005.05.013_bib24","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1099\/00222615-46-8-647","article-title":"Suckling CD1 mice as an animal model for studies of Legionella pneumophila virulence","volume":"46","author":"Castellani Pastoris","year":"1997","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.vaccine.2005.05.013_bib25","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1016\/j.vaccine.2005.05.013_bib26","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature (London)"},{"key":"10.1016\/j.vaccine.2005.05.013_bib27","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2005.05.013_bib28","doi-asserted-by":"crossref","first-page":"2585","DOI":"10.1128\/IAI.58.8.2585-2592.1990","article-title":"The Legionella pneumophila major secretory protein, a protease, is not required for intracellular growth or cell killing","volume":"58","author":"Szeto","year":"1990","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib29","first-page":"4.5.1","article-title":"In vivo assays for mouse lymphocyte function. 4.5 Delayed-Type Hypersesitivity","volume":"vol. 1","author":"Coligan","year":"1994"},{"key":"10.1016\/j.vaccine.2005.05.013_bib30","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1128\/JCM.29.5.953-956.1991","article-title":"Detection of flagella in 278 Legionella strains by latex reagent sensitized with antiflagellum immunoglobulins","volume":"29","author":"Bornstein","year":"1991","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.vaccine.2005.05.013_bib31","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1128\/IAI.33.2.602-610.1981","article-title":"Immunological and biochemical relationships among flagella isolated from Legionella pneumophila serogroups 1,2 and 3","volume":"33","author":"Elliott","year":"1981","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib32","doi-asserted-by":"crossref","first-page":"398","DOI":"10.1084\/jem.153.2.398","article-title":"Interaction of the Legionnaires\u2019 disease bacterium (Legionella pneumophila) with human phagocytes. II. Antibody promotes binding of L.pneumophila to monocytes but does not inhibit intracellular multiplication","volume":"153","author":"Horwitz","year":"1981","journal-title":"J Exp Med"},{"issue":"2","key":"10.1016\/j.vaccine.2005.05.013_bib33","doi-asserted-by":"crossref","first-page":"698","DOI":"10.1128\/IAI.26.2.698-704.1979","article-title":"Serospecificity and opsonic activity of antisera to Legionella pneumophila","volume":"26","author":"Johnson","year":"1979","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib34","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1128\/IAI.71.4.1622-1629.2003","article-title":"Flagella are virulence determinants of Burkholderia pseudomallei","volume":"71","author":"Chua","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib35","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1099\/0022-1317-51-11-958","article-title":"The Helicobacter pylori flbA flagellar biosynthesis and regulatory gene is required for motility and virulence and modulates urease of H. pylori and Proteus mirabilis","volume":"51","author":"McGee","year":"2002","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.vaccine.2005.05.013_bib36","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1099\/jmm.0.04901-0","article-title":"Lack of flagella disadvantages Salmonella enterica serovar Enteritidis during the early stages of infection in the rat","volume":"52","author":"Robertson","year":"2003","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.vaccine.2005.05.013_bib37","doi-asserted-by":"crossref","first-page":"1248","DOI":"10.4049\/jimmunol.166.2.1248","article-title":"Flagellin, a novel mediator of Salmonella-induced epithelial activation and systemic inflammation: I kappa B alpha degradation, induction of nitric oxide synthase, induction of proinflammatory mediators, and cardiovascular dysfunction","volume":"166","author":"Eaves-Pyles","year":"2001","journal-title":"J. Immunol"},{"key":"10.1016\/j.vaccine.2005.05.013_bib38","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.4049\/jimmunol.167.4.1882","article-title":"Cutting edge: bacterial flagellin activates basolaterallly expressed TLR5 to induce epithelial proinflammatory gene expression","volume":"167","author":"Gewirtz","year":"2001","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2005.05.013_bib39","doi-asserted-by":"crossref","first-page":"2546","DOI":"10.1128\/IAI.72.5.2546-2555.2004","article-title":"Host and bacterial factors affecting induction of immune responses to flagellin expressed by attenuated Salmonella vaccine strains","volume":"72","author":"Sbrogio-Almeida","year":"2004","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib40","series-title":"Legionella","first-page":"124","article-title":"Differential cytokine response following challenge of A\/J mice with virulent or avirulent Legionella pneumophila","author":"Torosantucci","year":"2002"},{"key":"10.1016\/j.vaccine.2005.05.013_bib41","doi-asserted-by":"crossref","first-page":"4151","DOI":"10.1128\/IAI.66.9.4151-4157.1998","article-title":"Low endotoxic potential of Legionella pneumophila lipopolysaccaride due to failure of interaction with the monocyte lipopolysaccaride receptor CD 14","volume":"66","author":"Neumeister","year":"1998","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.05.013_bib42","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1086\/341780","article-title":"Toll-like receptor 4 is not involved in host defence against pulmonary Legionella pneumophila infection in a mouse model","volume":"186","author":"Lettinga","year":"2002","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2005.05.013_bib43","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1084\/jem.20031220","article-title":"A common dominant TLR5 stop codon polymorphism abolishes flagellin signaling and is associated with susceptibility to legionnaires\u2019 disease","volume":"198","author":"Hawn","year":"2003","journal-title":"J Exp Med"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X05005323?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X05005323?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T16:01:15Z","timestamp":1586275275000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X05005323"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,9]]},"references-count":43,"journal-issue":{"issue":"40","published-print":{"date-parts":[[2005,9]]}},"alternative-id":["S0264410X05005323"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2005.05.013","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2005,9]]}}}