{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T19:34:56Z","timestamp":1775072096546,"version":"3.50.1"},"reference-count":41,"publisher":"Elsevier BV","issue":"13","license":[{"start":{"date-parts":[[2006,3,1]],"date-time":"2006-03-01T00:00:00Z","timestamp":1141171200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2006,3]]},"DOI":"10.1016\/j.vaccine.2005.11.062","type":"journal-article","created":{"date-parts":[[2005,12,20]],"date-time":"2005-12-20T10:21:35Z","timestamp":1135074095000},"page":"2358-2366","source":"Crossref","is-referenced-by-count":34,"title":["Vaccination with DNA encoding cysteine proteinase confers protective immune response to rats infected with Clonorchis sinensis"],"prefix":"10.1016","volume":"24","author":[{"given":"Ji-Sook","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"In Sik","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Woon-Mok","family":"Sohn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jongweon","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai-Soon","family":"Yong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2005.11.062_bib1","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1146\/annurev.immunol.15.1.617","article-title":"DNA vaccines","volume":"15","author":"Donnelly","year":"1997","journal-title":"Annu Rev Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib2","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1038\/icb.1997.58","article-title":"DNA vaccines for parasitic infections","volume":"75","author":"Kalinna","year":"1997","journal-title":"Immunol Cell Biol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib3","doi-asserted-by":"crossref","first-page":"2985","DOI":"10.1016\/S0264-410X(00)00095-5","article-title":"Successful DNA immunisation of rats against fasciolosis","volume":"18","author":"Kofta","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2005.11.062_bib4","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1006\/expr.2001.4601","article-title":"Fasciola: kinetics and quality of humoral responses to fatty acid binding protein and cathepsin l following delivery as DNA vaccines in mice","volume":"97","author":"Smooker","year":"2001","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib5","doi-asserted-by":"crossref","first-page":"4857","DOI":"10.1128\/IAI.71.9.4857-4861.2003","article-title":"A DNA vaccine encoding Cu, Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB\/c mice","volume":"71","author":"Onate","year":"2003","journal-title":"Infect Immun"},{"issue":"Suppl 1","key":"10.1016\/j.vaccine.2005.11.062_bib6","first-page":"47","article-title":"Epidemiology and control of clonorchiasis in Korea","volume":"28","author":"Rim","year":"1997","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"10.1016\/j.vaccine.2005.11.062_bib7","doi-asserted-by":"crossref","first-page":"397","DOI":"10.2307\/3285768","article-title":"How much human helminthiasis is there in the world?","volume":"85","author":"Crompton","year":"1999","journal-title":"J Parasitol"},{"issue":"Supp 4","key":"10.1016\/j.vaccine.2005.11.062_bib8","doi-asserted-by":"crossref","first-page":"308","DOI":"10.1023\/A:1008313809752","article-title":"Risk factors for biliary tract carcinogenesis","volume":"10","author":"Chapman","year":"1999","journal-title":"Ann Oncol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib9","first-page":"21","article-title":"Effect of Clonorchis sinensis infection and dimethylnitrosamine administration on the induction of cholangiocarcinoma in Syrian golden hamster","volume":"31","author":"Kim","year":"1993","journal-title":"Korean J Pathol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib10","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1146\/annurev.mi.47.100193.004133","article-title":"The proteases and pathogenicity of parasitic protozoa","volume":"47","author":"McKerrow","year":"1993","journal-title":"Annu Rev Microbiol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib11","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1128\/IAI.67.1.368-374.1999","article-title":"Recombinant expression and localization of Schistosoma mansoni cathepsin L1 support its role in the degradation of host hemoglobin","volume":"67","author":"Brady","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.11.062_bib12","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1432-1033.1995.tb20805.x","article-title":"Fasciola hepatica cathepsin L proteinase cleaves fibrinogen and produces a novel type of fibrin clot","volume":"232","author":"Dowd","year":"1995","journal-title":"Eur J Biochem"},{"key":"10.1016\/j.vaccine.2005.11.062_bib13","doi-asserted-by":"crossref","first-page":"211","DOI":"10.3347\/kjp.1995.33.3.211","article-title":"Cytotoxicity of a cysteine proteinase of adult Clonorchis sinensis","volume":"33","author":"Park","year":"1995","journal-title":"Korean J Parasitol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib14","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0305-0491(91)90018-9","article-title":"Cysteine proteinase activity in various developmental stages of Clonorchis sinensis: a comparative analysis","volume":"99","author":"Song","year":"1991","journal-title":"Comp Biochem Physiol B"},{"key":"10.1016\/j.vaccine.2005.11.062_bib15","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1128\/CDLI.8.6.1076-1080.2001","article-title":"Cystatin capture enzyme-linked immunosorbent assay for serodiagnosis of human clonorchiasis and profile of captured antigenic protein of Clonorchis sinensis","volume":"8","author":"Kim","year":"2001","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib16","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1645\/0022-3395(2002)088[1000:EOCPOC]2.0.CO;2","article-title":"Expression of cysteine proteinase of Clonorchis sinensis and its use in serodiagnosis of clonorchiasis","volume":"88","author":"Na","year":"2002","journal-title":"J Parasitol"},{"issue":"8","key":"10.1016\/j.vaccine.2005.11.062_bib17","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1016\/0020-7519(93)90117-H","article-title":"Fasciola hepatica: a secreted cathepsin L-like proteinase cleaves host immunoglobulin","volume":"23","author":"Smith","year":"1993","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib18","doi-asserted-by":"crossref","first-page":"5066","DOI":"10.1128\/IAI.64.12.5066-5074.1996","article-title":"Induction of protective immunity in cattle against infection with Fasciola hepatica by vaccination with cathepsin L proteinases and with hemoglobin","volume":"64","author":"Dalton","year":"1996","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.11.062_bib19","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1006\/expr.1998.4234","article-title":"Fasciola hepatica: characterization and cloning of the major cathepsin B protease secreted by newly excysted juvenile liver fluke","volume":"88","author":"Wilson","year":"1998","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib20","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1053\/rvsc.2000.0425","article-title":"Cathepsin L proteinases as vaccines against infection with Fasciola hepatica (liver fluke) in ruminants","volume":"70","author":"Mulcahy","year":"2001","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.vaccine.2005.11.062_bib21","doi-asserted-by":"crossref","first-page":"2439","DOI":"10.1016\/S0264-410X(02)00189-5","article-title":"Recombinant cysteine proteinases-based vaccines against Leishmania major in BALB\/c mice: the partial protection relies on interferon gamma producing CD8(+) T lymphocyte activation","volume":"20","author":"Rafati","year":"2002","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2005.11.062_bib22","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1128\/IAI.69.1.386-391.2001","article-title":"Vaccination with calpain induces a Th1-biased protective immune response against Schistosoma japonicum","volume":"69","author":"Zhang","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2005.11.062_bib23","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1007\/s00436-003-0962-y","article-title":"Analysis of the genes expressed in Clonorchis sinensis adults using the expressed sequence tag approach","volume":"91","author":"Lee","year":"2003","journal-title":"Parasitol Res"},{"key":"10.1016\/j.vaccine.2005.11.062_bib24","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein\u2013dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal Biochem"},{"key":"10.1016\/j.vaccine.2005.11.062_bib25","first-page":"495","article-title":"Immunoblotting in the clinical laboratory","volume":"27","author":"Towbin","year":"1989","journal-title":"J Clin Chem Clin Biochem"},{"key":"10.1016\/j.vaccine.2005.11.062_bib26","first-page":"326","article-title":"An ether sedimentation technique for routine stool examination","volume":"8","author":"Ritchie","year":"1948","journal-title":"Bull US Army Med Dept"},{"key":"10.1016\/j.vaccine.2005.11.062_bib27","first-page":"59","article-title":"An effective method of counting hookworm egg in feces","volume":"3","author":"Stoll","year":"1923","journal-title":"Am J Hyg"},{"key":"10.1016\/j.vaccine.2005.11.062_bib28","series-title":"Antibodies","author":"Harlow","year":"1988"},{"key":"10.1016\/j.vaccine.2005.11.062_bib29","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1046\/j.1365-3024.2002.00461.x","article-title":"Vaccination of calves against Ostertagia ostertagi with cysteine proteinase enriched protein fractions","volume":"24","author":"Geldhof","year":"2002","journal-title":"Parasite Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib30","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/0305-0491(91)90018-9","article-title":"Cysteine proteinase activity in various developmental stages of Clonorchis sinensis: a comparative analysis","volume":"99","author":"Song","year":"1991","journal-title":"Comp Biochem Physiol B"},{"key":"10.1016\/j.vaccine.2005.11.062_bib31","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/S0165-2427(03)00085-0","article-title":"The immune response of rats to vaccination with the cDNA or protein forms of the cysteine proteinase of Fasciola hepatica","volume":"94","author":"Wedrychowicz","year":"2003","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib32","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0014-4894(03)00036-5","article-title":"Characterization and classification of five cysteine proteinases expressed by Paragonimus westermani adult worm","volume":"102","author":"Park","year":"2002","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib33","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1006\/smim.1997.0083","article-title":"DNA vaccines","volume":"9","author":"Robinson","year":"1997","journal-title":"Semin Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib34","first-page":"165","article-title":"CpG motifs for optimization of DNA vaccines","volume":"104","author":"Davis","year":"2000","journal-title":"Dev Biol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib35","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1016\/S0952-7915(00)00118-7","article-title":"DNA vaccines: a key for inducing long-term cellular immunity","volume":"12","author":"Gurunathan","year":"2000","journal-title":"Curr Opin Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib36","doi-asserted-by":"crossref","first-page":"5141","DOI":"10.1073\/pnas.93.10.5141","article-title":"Preferential induction of a Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization","volume":"93","author":"Raz","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2005.11.062_bib37","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1093\/intimm\/9.12.1897","article-title":"Influence of cellular location of expressed antigen on the efficacy of DNA vaccination: cytotoxic T lymphocyte and antibody responses are suboptimal when antigen is cytoplasmic after intramuscular DNA immunization","volume":"9","author":"Boyle","year":"1997","journal-title":"Int Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib38","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.4049\/jimmunol.171.4.1999","article-title":"DNA vaccines, combining form of antigen and method of delivery to raise a spectrum of IFN-gamma and IL-4-producing CD4+ and CD8+ T cells","volume":"171","author":"Oran","year":"2003","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib39","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1046\/j.1365-3024.1997.d01-163.x","article-title":"Intradermal immunization of rats with plasmid DNA encoding Schistosoma mansoni 28 kDa glutathione S-transferase","volume":"19","author":"Dupre","year":"1997","journal-title":"Parasite Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib40","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/S0065-2776(08)60652-5","article-title":"Heterogeneity of cytokine secretion patterns and functions of helper T cells","volume":"46","author":"Mosmann","year":"1989","journal-title":"Adv Immunol"},{"key":"10.1016\/j.vaccine.2005.11.062_bib41","doi-asserted-by":"crossref","first-page":"2826","DOI":"10.4049\/jimmunol.138.9.2826","article-title":"Polyclonal activation of the murine immune system by a goat antibody to mouse IgD. IX. Induction of a polyclonal IgE response","volume":"138","author":"Finkelman","year":"1987","journal-title":"J Immunol"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X0501203X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X0501203X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,5]],"date-time":"2023-05-05T19:16:41Z","timestamp":1683314201000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X0501203X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,3]]},"references-count":41,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2006,3]]}},"alternative-id":["S0264410X0501203X"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2005.11.062","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2006,3]]}}}