{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T12:11:17Z","timestamp":1777983077863,"version":"3.51.4"},"reference-count":42,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2007,3]]},"DOI":"10.1016\/j.vaccine.2006.12.004","type":"journal-article","created":{"date-parts":[[2006,12,16]],"date-time":"2006-12-16T07:14:37Z","timestamp":1166253277000},"page":"2261-2268","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":43,"title":["Nasal immunization with recombinant Brucella melitensis bp26 and trigger factor with cholera toxin reduces B. melitensis colonization"],"prefix":"10.1016","volume":"25","author":[{"given":"Xinghong","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Walters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda","family":"Robison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theresa","family":"Trunkle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David W.","family":"Pascual","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2006.12.004_bib1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.vaccine.2006.12.004_bib2","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/S1286-4579(01)01427-7","article-title":"Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the omp2 locus","volume":"3","author":"Cloeckaert","year":"2001","journal-title":"Microbes Infect"},{"key":"10.1016\/j.vaccine.2006.12.004_bib3","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1186\/1471-2105-7-347","article-title":"BBP: Brucella genome annotation with literature mining and curation","volume":"16","author":"Xiang","year":"2006","journal-title":"BMC Bioinformatics"},{"key":"10.1016\/j.vaccine.2006.12.004_bib4","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/0264-410X(96)00018-7","article-title":"Immunization of mice with recombinant L7\/L12 ribosomal protein confers protection against Brucella abortus infection","volume":"14","author":"Oliveira","year":"1996","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2006.12.004_bib5","doi-asserted-by":"crossref","first-page":"4857","DOI":"10.1128\/IAI.71.9.4857-4861.2003","article-title":"A DNA vaccine encoding Cu, Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB\/c mice","volume":"71","author":"O\u00f1ate","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib6","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1128\/IAI.72.4.2081-2087.2004","article-title":"Intraspleen delivery of a DNA vaccine coding for superoxide dismutase (SOD) of Brucella abortus induces SOD-specific CD4+and CD8+ T cells","volume":"72","author":"Munoz-Montesino","year":"2004","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib7","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.imbio.2005.09.004","article-title":"Evaluation of Brucella abortus DNA vaccine by expression of Cu-Zn superoxide dismutase antigen fused to IL-2","volume":"211","author":"Gonzalez-Smith","year":"2006","journal-title":"Immunobiology"},{"key":"10.1016\/j.vaccine.2006.12.004_bib8","doi-asserted-by":"crossref","first-page":"2507","DOI":"10.1128\/IAI.70.5.2507-2511.2002","article-title":"A DNA vaccine encoding lumazine synthase from Brucella abortus induces protective immunity in BALB\/c mice","volume":"70","author":"Velikovsky","year":"2002","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib9","doi-asserted-by":"crossref","first-page":"6264","DOI":"10.1128\/IAI.69.10.6264-6270.2001","article-title":"Induction of immune response in BALB\/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp.","volume":"69","author":"Al-Mariri","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib10","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1128\/IAI.74.5.2734-2741.2006","article-title":"Protective immunity elicited by a divalent DNA vaccine encoding both the L7\/L12 and Omp16 genes of Brucella abortus in BALB\/c mice","volume":"74","author":"Luo","year":"2006","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib11","doi-asserted-by":"crossref","first-page":"6537","DOI":"10.1128\/IAI.73.10.6537-6546.2005","article-title":"A DNA vaccine coding for the Brucella outer membrane protein 31 confers protection against B. melitensis and B. ovis infection by eliciting a specific cytotoxic response","volume":"73","author":"Cassataro","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib12","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1099\/0022-1317-51-8-661","volume":"51","author":"Rosinha","year":"2002","journal-title":"J Med Microbiol"},{"key":"10.1016\/j.vaccine.2006.12.004_bib13","doi-asserted-by":"crossref","first-page":"7297","DOI":"10.1128\/IAI.73.11.7297-7303.2005","article-title":"Selection of protective epitopes for Brucella melitensis using DNA vaccination","volume":"73","author":"Yang","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib14","doi-asserted-by":"crossref","first-page":"3836","DOI":"10.1016\/j.vaccine.2005.02.023","article-title":"Transgene vaccination using Ulex europaeus agglutinin I (UEA-1) for targeted mucosal immunization against HIV-1 envelope","volume":"23","author":"Wang","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2006.12.004_bib15","doi-asserted-by":"crossref","first-page":"3029","DOI":"10.1002\/1521-4141(200211)32:11<3029::AID-IMMU3029>3.0.CO;2-9","article-title":"Mucosal addressin expression and binding interactions with native lymphocytes vary among the cranial, oral, and nasal associated lymphoid tissues","volume":"32","author":"Csencsits","year":"2002","journal-title":"Eur J Immunol"},{"key":"10.1016\/j.vaccine.2006.12.004_bib16","doi-asserted-by":"crossref","first-page":"895","DOI":"10.1089\/hum.1995.6.7-895","article-title":"Intratracheal gene delivery with adenoviral vector induces elevated systemic IgG and mucosal IgA antibodies to adenovirus and \u03b2-galactosidase","volume":"6","author":"van Ginkel","year":"1995","journal-title":"Hum Gene Ther"},{"key":"10.1016\/j.vaccine.2006.12.004_bib17","doi-asserted-by":"crossref","first-page":"5470","DOI":"10.1128\/IAI.66.11.5470-5476.1998","article-title":"Oral immunization with a Salmonella typhimurium vaccine vector expressing recombinant enterotoxigenic Escherichia coli K99 fimbriae elicits elevated antibody titers for protective immunity","volume":"66","author":"Asc\u00f3n","year":"1998","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib18","doi-asserted-by":"crossref","first-page":"2325","DOI":"10.1056\/NEJMra050570","volume":"352","author":"Pappas","year":"2005","journal-title":"N Engl J Med"},{"key":"10.1016\/j.vaccine.2006.12.004_bib19","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1001\/jama.1997.03550050061035","article-title":"Clinical recognition and management of patients exposed to biological warfare agents","volume":"278","author":"Franz","year":"1997","journal-title":"JAMA"},{"key":"10.1016\/j.vaccine.2006.12.004_bib20","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1093\/infdis\/170.5.1216","article-title":"Changing trends in the epidemiology of human brucellosis in California from 1973 to 1992: a shift toward foodborne transmission","volume":"170","author":"Chomel","year":"1994","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2006.12.004_bib21","first-page":"548","article-title":"Epidemiologic notes and reports brucellosis\u2014Texas","volume":"32","year":"1983","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"10.1016\/j.vaccine.2006.12.004_bib22","first-page":"113","article-title":"Brucellosis outbreak at a pork processing plant\u2014North Carolina","volume":"43","year":"1994","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"10.1016\/j.vaccine.2006.12.004_bib23","first-page":"172","article-title":"Human exposure to Brucella abortus strain RB51\u2014Kansas","volume":"47","year":"1997","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"10.1016\/j.vaccine.2006.12.004_bib24","doi-asserted-by":"crossref","first-page":"375","DOI":"10.4269\/ajtmh.1997.56.375","article-title":"A clinical study of brucellosis in adults in the Asir region of southern Saudi Arabia","volume":"56","author":"Malik","year":"1997","journal-title":"Am J Trop Med Hyg"},{"key":"10.1016\/j.vaccine.2006.12.004_bib25","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1093\/clinids\/20.5.1241","article-title":"Characteristics of and risk factors for relapse of brucellosis in humans","volume":"20","author":"Ariza","year":"1995","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.vaccine.2006.12.004_bib26","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1016\/j.vaccine.2005.08.003","article-title":"One-time intradermal DNA vaccination in ear pinnae one year prior to infection protects dogs against rabies virus","volume":"24","author":"Lodmell","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2006.12.004_bib27","doi-asserted-by":"crossref","first-page":"3705","DOI":"10.1016\/j.vaccine.2005.07.012","article-title":"DNA vaccines against enteric infections","volume":"24","author":"Herrmann","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2006.12.004_bib28","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1080\/08830180600785827","article-title":"From plasmids to protection: a review of DNA vaccines against infectious diseases","volume":"25","author":"Laddy","year":"2006","journal-title":"Int Rev Immunol"},{"key":"10.1016\/j.vaccine.2006.12.004_bib29","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0378-1135(96)01265-5","article-title":"Competitive enzyme-linked immunosorbent assay using monoclonal antibodies to the Brucella melitensis BP26 protein to evaluate antibody responses in infected and B. melitensis Rev1-vaccinated sheep","volume":"53","author":"Debbarh","year":"1996","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2006.12.004_bib30","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1128\/cdli.3.3.305-308.1996","article-title":"Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev.1-vaccinated sheep","volume":"3","author":"Salih-Alj","year":"1996","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.vaccine.2006.12.004_bib31","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1016\/j.vaccine.2004.01.001","article-title":"Development and evaluation as vaccines in mice of Brucella melitensis Rev. 1 single and double deletion mutants of the bp26 and omp31 genes coding for antigens of diagnostic significance in ovine brucellosis","volume":"22","author":"Cloeckaert","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2006.12.004_bib32","first-page":"1495","article-title":"Presence of antigen specific long-term memory cells in systemic lymphoid tissues as well as locally in the gut lamina propria following oral immunization with cholera toxin adjuvant","volume":"371B","author":"Vajdy","year":"1995","journal-title":"Adv Exp Med Biol"},{"key":"10.1016\/j.vaccine.2006.12.004_bib33","doi-asserted-by":"crossref","first-page":"1516","DOI":"10.1128\/iai.64.5.1516-1525.1996","article-title":"Oral immunization of interleukin-4 (IL-4) knockout mice with a recombinant Salmonella strain or cholera toxin reveals that CD4+ Th2 cells producing IL-6 and IL-10 are associated with mucosal immunoglobulin A responses","volume":"64","author":"Okahashi","year":"1996","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib34","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1086\/315694","article-title":"Enterotoxin adjuvants have direct effects on T cells and antigen-presenting cells that result in either interleukin-4-dependent or -independent immune responses","volume":"82","author":"Yamamoto","year":"2000","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2006.12.004_bib35","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1084\/jem.178.4.1309","article-title":"Helper T cell subsets for immunoglobulin A responses: oral immunization with tetanus toxoid and cholera toxin as adjuvant selectively induces Th2 cells in mucosa associated tissues","volume":"178","author":"Xu-Amano","year":"1993","journal-title":"J Exp Med"},{"key":"10.1016\/j.vaccine.2006.12.004_bib36","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1084\/jem.172.1.95","article-title":"Cholera toxin discriminates between T helper 1 and 2 cells in T cell receptor-mediated activation: role of cAMP in T cell proliferation","volume":"172","author":"Munoz","year":"1990","journal-title":"J Exp Med"},{"key":"10.1016\/j.vaccine.2006.12.004_bib37","doi-asserted-by":"crossref","first-page":"7015","DOI":"10.4049\/jimmunol.162.12.7015","article-title":"Direct effects on antigen-presenting cells and T lymphocytes explain the adjuvanticity of a nontoxic cholera toxin mutant","volume":"162","author":"Yamamoto","year":"1999","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2006.12.004_bib38","doi-asserted-by":"crossref","first-page":"4899","DOI":"10.1128\/iai.61.11.4899-4901.1993","article-title":"Endogenous gamma interferon mediates resistance to Brucella abortus infection","volume":"61","author":"Zhan","year":"1993","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib39","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1128\/iai.61.1.124-134.1993","article-title":"Effects of cytokines on intracellular growth of Brucella abortus","volume":"61","author":"Jiang","year":"1993","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib40","doi-asserted-by":"crossref","first-page":"5267","DOI":"10.1128\/iai.62.12.5267-5274.1994","article-title":"Live Brucella spp. fail to induce tumor necrosis factor alpha excretion upon infection of U937-derived phagocytes","volume":"62","author":"Caron","year":"1994","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2006.12.004_bib41","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.4049\/jimmunol.156.8.2885","article-title":"Brucella species release a specific, protease-sensitive, inhibitor of TNF-\u03b1 expression, active on human macrophage-like cells","volume":"156","author":"Caron","year":"1996","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2006.12.004_bib42","doi-asserted-by":"crossref","first-page":"4990","DOI":"10.1128\/iai.62.11.4990-4996.1994","article-title":"Vaccination with Brucella abortus rough mutant RB51 protects BALB\/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis","volume":"62","author":"Jim\u00e9nez de Bag\u00fc\u00e9s","year":"1994","journal-title":"Infect Immun"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X0601320X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X0601320X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:15:12Z","timestamp":1759400112000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X0601320X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":42,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["S0264410X0601320X"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2006.12.004","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2007,3]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Nasal immunization with recombinant Brucella melitensis bp26 and trigger factor with cholera toxin reduces B. melitensis colonization","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2006.12.004","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2006 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}