{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T03:54:45Z","timestamp":1780631685802,"version":"3.54.1"},"reference-count":49,"publisher":"Elsevier BV","issue":"51","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2007,12]]},"DOI":"10.1016\/j.vaccine.2007.09.074","type":"journal-article","created":{"date-parts":[[2007,11,14]],"date-time":"2007-11-14T22:23:59Z","timestamp":1195079039000},"page":"8574-8584","source":"Crossref","is-referenced-by-count":24,"title":["Construction and preclinical evaluation of recombinant Peru-15 expressing high levels of the cholera toxin B subunit as a vaccine against enterotoxigenic Escherichia coli"],"prefix":"10.1016","volume":"25","author":[{"given":"Kenneth L.","family":"Roland","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl","family":"Cloninger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sims K.","family":"Kochi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lawrence J.","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven A.","family":"Tinge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Rouskey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin P.","family":"Killeen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"Suppl 1","key":"10.1016\/j.vaccine.2007.09.074_bib1","doi-asserted-by":"crossref","first-page":"S73","DOI":"10.1093\/clinids\/12.Supplement_1.S73","article-title":"Epidemiology of travelers\u2019 diarrhea and relative importance of various pathogens","volume":"12","author":"Black","year":"1990","journal-title":"Rev Infect Dis"},{"issue":"11","key":"10.1016\/j.vaccine.2007.09.074_bib2","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/0966-842X(96)10068-8","article-title":"Colonization factors of human enterotoxigenic Escherichia coli (ETEC)","volume":"4","author":"Gaastra","year":"1996","journal-title":"Trends Microbiol"},{"issue":"11","key":"10.1016\/j.vaccine.2007.09.074_bib3","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1086\/340419","article-title":"Natural history of enteroaggregative and enterotoxigenic Escherichia coli infection among US travelers to Guadalajara, Mexico","volume":"185","author":"Adachi","year":"2002","journal-title":"J Infect Dis"},{"issue":"3","key":"10.1016\/j.vaccine.2007.09.074_bib4","doi-asserted-by":"crossref","first-page":"137","DOI":"10.2310\/7060.2002.23206","article-title":"Enterotoxigenic Escherichia coli as cause of diarrhea among Mexican adults and US travelers in Mexico","volume":"9","author":"Bouckenooghe","year":"2002","journal-title":"J Travel Med"},{"issue":"4","key":"10.1016\/j.vaccine.2007.09.074_bib5","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1086\/338834","article-title":"Prevalence of enteric pathogens among international travelers with diarrhea acquired in Kenya (Mombasa), India (Goa), or Jamaica (Montego Bay)","volume":"185","author":"Jiang","year":"2002","journal-title":"J Infect Dis"},{"issue":"4","key":"10.1016\/j.vaccine.2007.09.074_bib6","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1128\/JCM.31.4.851-856.1993","article-title":"Characterization of enterotoxigenic Escherichia coli isolated from US troops deployed to the Middle East","volume":"31","author":"Wolf","year":"1993","journal-title":"J Clin Microbiol"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib7","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1128\/JCM.38.1.27-31.2000","article-title":"Prevalence of toxin types and colonization factors in enterotoxigenic Escherichia coli isolated during a 2-year period from diarrheal patients in Bangladesh","volume":"38","author":"Qadri","year":"2000","journal-title":"J Clin Microbiol"},{"issue":"2","key":"10.1016\/j.vaccine.2007.09.074_bib8","doi-asserted-by":"crossref","first-page":"120","DOI":"10.4269\/ajtmh.2001.65.120","article-title":"Toxins and colonization factor antigens of enterotoxigenic Escherichia coli among residents of Jakarta Indonesia","volume":"65","author":"Oyofo","year":"2001","journal-title":"Am J Trop Med Hyg"},{"issue":"6","key":"10.1016\/j.vaccine.2007.09.074_bib9","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/S1473-3099(05)70139-0","article-title":"Traveller's diarrhoea","volume":"5","author":"Al-Abri","year":"2005","journal-title":"Lancet Infect Dis"},{"issue":"6","key":"10.1016\/j.vaccine.2007.09.074_bib10","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1128\/JCM.35.6.1639-1641.1997","article-title":"Coli surface antigens associated with enterotoxigenic Escherichia coli strains isolated from persons with traveler's diarrhea in Asia","volume":"35","author":"Serichantalergs","year":"1997","journal-title":"J Clin Microbiol"},{"issue":"Suppl 8","key":"10.1016\/j.vaccine.2007.09.074_bib11","doi-asserted-by":"crossref","first-page":"S536","DOI":"10.1086\/432948","article-title":"Epidemiology of traveler's diarrhea","volume":"41","author":"Steffen","year":"2005","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.vaccine.2007.09.074_bib12","series-title":"The Comprehensive Sourcebook of Bacterial Protein Toxins","first-page":"104","article-title":"Cholera toxin and Escherichia coli heat-labile enterotoxin","author":"Hirst","year":"1999"},{"issue":"2","key":"10.1016\/j.vaccine.2007.09.074_bib13","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1093\/infdis\/158.2.372","article-title":"Cross-protection by B subunit-whole cell cholera vaccine against diarrhea associated with heat-labile toxin-producing enterotoxigenic Escherichia coli: results of a large-scale field trial","volume":"158","author":"Clemens","year":"1988","journal-title":"J Infect Dis"},{"issue":"8778","key":"10.1016\/j.vaccine.2007.09.074_bib14","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1016\/0140-6736(91)92590-X","article-title":"Prevention of travellers\u2019 diarrhoea by oral B-subunit\/whole-cell cholera vaccine","volume":"338","author":"Peltola","year":"1991","journal-title":"Lancet"},{"issue":"4","key":"10.1016\/j.vaccine.2007.09.074_bib15","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1128\/CMR.10.4.569","article-title":"Occurrence, distribution, and associations of O and H serogroups, colonization factor antigens, and toxins of enterotoxigenic Escherichia coli","volume":"10","author":"Wolf","year":"1997","journal-title":"Clin Microbiol Rev"},{"issue":"9380","key":"10.1016\/j.vaccine.2007.09.074_bib16","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S0140-6736(03)13971-2","article-title":"Protection from natural infections with enterotoxigenic Escherichia coli: longitudinal study","volume":"362","author":"Steinsland","year":"2003","journal-title":"Lancet"},{"issue":"3","key":"10.1016\/j.vaccine.2007.09.074_bib17","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1128\/CMR.18.3.465-483.2005","article-title":"Enterotoxigenic Escherichia coli in developing countries: epidemiology, microbiology, clinical features, treatment, and prevention","volume":"18","author":"Qadri","year":"2005","journal-title":"Clin Microbiol Rev"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib18","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1128\/IAI.50.1.255-261.1985","article-title":"Experimental enterotoxin-induced Escherichia coli diarrhea and protection induced by previous infection with bacteria of the same adhesin or enterotoxin type","volume":"50","author":"Ahren","year":"1985","journal-title":"Infect Immunol"},{"issue":"5","key":"10.1016\/j.vaccine.2007.09.074_bib19","doi-asserted-by":"crossref","first-page":"247","DOI":"10.2310\/7060.2002.24210","article-title":"Intestinal immunoglobulin a response to naturally acquired enterotoxigenic Escherichia coli in US travelers to an endemic area of Mexico","volume":"9","author":"Estrada-Garcia","year":"2002","journal-title":"J Travel Med"},{"key":"10.1016\/j.vaccine.2007.09.074_bib20","series-title":"Relationship between Enterotoxigenic Escherichia coli and Travelers\u2019 Diarrhea in Mexico, from 1992 to 1997","author":"Jiang","year":"1998"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib21","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.diagmicrobio.2005.12.002","article-title":"Enterotoxigenic Escherichia coli colonization factor types collected from 1997 to 2001 in US military personnel during operation Bright Star in northern Egypt","volume":"55","author":"Rockabrand","year":"2006","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"2","key":"10.1016\/j.vaccine.2007.09.074_bib22","doi-asserted-by":"crossref","first-page":"102","DOI":"10.2310\/7060.2005.12207","article-title":"Vaccination against enterotoxigenic Escherichia coli, a cause of travelers\u2019 diarrhea","volume":"12","author":"Steffen","year":"2005","journal-title":"J Travel Med"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib23","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1086\/514025","article-title":"Evaluation of Peru-15, a new live oral vaccine for cholera, in volunteers","volume":"176","author":"Sack","year":"1997","journal-title":"J Infect Dis"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib24","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1111\/j.1708-8305.1995.tb00615.x","article-title":"Safety, Immunogenicity, and Protective Efficacy of the Whole-Cell\/Recombinant B Subunit (WC\/rBS) Oral Cholera Vaccine Against Travelers\u2019 Diarrhea","volume":"2","author":"Scerpella","year":"1995","journal-title":"J Travel Med"},{"issue":"43","key":"10.1016\/j.vaccine.2007.09.074_bib25","doi-asserted-by":"crossref","first-page":"5120","DOI":"10.1016\/j.vaccine.2005.05.022","article-title":"Analysis of efficacy of CVD 103-HgR live oral cholera vaccine against all-cause travellers\u2019 diarrhoea in a randomised, double-blind, placebo-controlled study","volume":"23","author":"Leyten","year":"2005","journal-title":"Vaccine"},{"issue":"6","key":"10.1016\/j.vaccine.2007.09.074_bib26","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/0264-410X(90)90012-B","article-title":"Randomized double-blind placebo controlled trial to evaluate the safety and immunogenicity of the live oral cholera vaccine strain CVD 103-HgR in Swiss adults","volume":"8","author":"Cryz","year":"1990","journal-title":"Vaccine"},{"issue":"24","key":"10.1016\/j.vaccine.2007.09.074_bib27","doi-asserted-by":"crossref","first-page":"7752","DOI":"10.1073\/pnas.81.24.7752","article-title":"Mechanism of toxin secretion by Vibrio cholerae investigated in strains harboring plasmids that encode heat-labile enterotoxins of Escherichia coli","volume":"81","author":"Hirst","year":"1984","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib28","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1128\/IAI.68.1.221-226.2000","article-title":"Development of a Delta glnA balanced lethal plasmid system for expression of heterologous antigens by attenuated vaccine vector strains of Vibrio cholerae","volume":"68","author":"Ryan","year":"2000","journal-title":"Infect Immunol"},{"issue":"3","key":"10.1016\/j.vaccine.2007.09.074_bib29","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1046\/j.1365-2958.1998.00809.x","article-title":"Distinct roles of an alternative sigma factor during both free-swimming and colonizing phases of the Vibrio cholerae pathogenic cycle","volume":"28","author":"Klose","year":"1998","journal-title":"Mol Microbiol"},{"issue":"4","key":"10.1016\/j.vaccine.2007.09.074_bib30","doi-asserted-by":"crossref","first-page":"1126","DOI":"10.1093\/infdis\/172.4.1126","article-title":"Peru-15, an improved live attenuated oral vaccine candidate for Vibrio cholerae O1","volume":"172","author":"Kenner","year":"1995","journal-title":"J Infect Dis"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib31","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/0378-1119(85)90120-9","article-title":"Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors","volume":"33","author":"Yanisch-Perron","year":"1985","journal-title":"Gene"},{"issue":"8","key":"10.1016\/j.vaccine.2007.09.074_bib32","doi-asserted-by":"crossref","first-page":"3118","DOI":"10.1128\/IAI.65.8.3118-3125.1997","volume":"65","author":"Ryan","year":"1997","journal-title":"Infect Immunol"},{"issue":"10","key":"10.1016\/j.vaccine.2007.09.074_bib33","doi-asserted-by":"crossref","first-page":"7066","DOI":"10.1073\/pnas.092152899","article-title":"Identification of a protein secretory pathway for the secretion of heat-labile enterotoxin by an enterotoxigenic strain of Escherichia coli","volume":"99","author":"Tauschek","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"issue":"4","key":"10.1016\/j.vaccine.2007.09.074_bib34","first-page":"58","article-title":"Optimization of a rabbit model of attenuated Vibrio cholerae immunogenicity","volume":"43","author":"Linnetz","year":"2004","journal-title":"Contemp. Topics"},{"issue":"5","key":"10.1016\/j.vaccine.2007.09.074_bib35","doi-asserted-by":"crossref","first-page":"2163","DOI":"10.1128\/IAI.01740-06","article-title":"Safety and immunogenicity of an enterotoxigenic Escherichia coli vaccine patch containing heat-labile toxin: use of skin pretreatment to disrupt the stratum corneum","volume":"75","author":"Glenn","year":"2007","journal-title":"Infect Immunol"},{"issue":"23-24","key":"10.1016\/j.vaccine.2007.09.074_bib36","doi-asserted-by":"crossref","first-page":"3061","DOI":"10.1016\/j.vaccine.2004.02.019","article-title":"Recombinant cholera toxin B subunit (rCTB) as a mucosal adjuvant enhances induction of diphtheria and tetanus antitoxin antibodies in mice by intranasal administration with diphtheria-pertussis-tetanus (DPT) combination vaccine","volume":"22","author":"Isaka","year":"2004","journal-title":"Vaccine"},{"issue":"11","key":"10.1016\/j.vaccine.2007.09.074_bib37","doi-asserted-by":"crossref","first-page":"4137","DOI":"10.4049\/jimmunol.156.11.4137","article-title":"Distinct effects of recombinant cholera toxin B subunit and holotoxin on different stages of class II MHC antigen processing and presentation by macrophages","volume":"156","author":"Matousek","year":"1996","journal-title":"J Immunol"},{"issue":"5","key":"10.1016\/j.vaccine.2007.09.074_bib38","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1128\/IAI.69.5.3488-3493.2001","article-title":"Preparation, immunogenicity, and protective efficacy, in a murine model, of a conjugate vaccine composed of the polysaccharide moiety of the lipopolysaccharide of Vibrio cholerae O139 bound to tetanus toxoid","volume":"69","author":"Boutonnier","year":"2001","journal-title":"Infect Immunol"},{"issue":"Suppl 2","key":"10.1016\/j.vaccine.2007.09.074_bib39","article-title":"Preparation and evaluation of Vibrio cholerae O1 EL Tor Ogawa lipopolysaccharide-tetanus toxoid conjugates","volume":"24","author":"Cabrera","year":"2006","journal-title":"Vaccine"},{"issue":"5","key":"10.1016\/j.vaccine.2007.09.074_bib40","doi-asserted-by":"crossref","first-page":"2021","DOI":"10.1128\/IAI.63.5.2021-2025.1995","article-title":"Local and systemic antibody responses to dextran-cholera toxin B subunit conjugates","volume":"63","author":"Bergquist","year":"1995","journal-title":"Infect Immunol"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib41","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1046\/j.1365-2567.1997.00314.x","article-title":"Immunogenicity of bacterial carbohydrates: cholera toxin modulates the immune response against dextran B512","volume":"92","author":"Sverremark","year":"1997","journal-title":"Immunology"},{"issue":"8","key":"10.1016\/j.vaccine.2007.09.074_bib42","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/S0966-842X(01)02096-0","article-title":"Can a \u2018flawless\u2019 live vector vaccine strain be engineered?","volume":"9","author":"Galen","year":"2001","journal-title":"Trends Microbiol"},{"issue":"6","key":"10.1016\/j.vaccine.2007.09.074_bib43","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.ijmm.2004.07.010","article-title":"Use of the alpha-hemolysin secretion system of Escherichia coli for antigen delivery in the Salmonella typhi Ty21a vaccine strain","volume":"294","author":"Gentschev","year":"2004","journal-title":"Int J Med Microbiol"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib44","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1128\/IAI.73.1.362-368.2005","article-title":"Optimization of Salmonella enterica serovar Typhi Delta aroC Delta ssaV derivatives as vehicles for delivering heterologous antigens by chromosomal integration and in vivo inducible promoters","volume":"73","author":"Stratford","year":"2005","journal-title":"Infect Immunol"},{"issue":"21","key":"10.1016\/j.vaccine.2007.09.074_bib45","doi-asserted-by":"crossref","first-page":"4175","DOI":"10.1016\/j.vaccine.2007.03.007","article-title":"Ability of SPI2 mutant of S. typhi to effectively induce antibody responses to the mucosal antigen enterotoxigenic E. coli heat labile toxin B subunit after oral delivery to humans","volume":"25","author":"Khan","year":"2007","journal-title":"Vaccine"},{"issue":"2","key":"10.1016\/j.vaccine.2007.09.074_bib46","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1128\/IAI.46.2.564-569.1984","article-title":"Construction of a potential live oral bivalent vaccine for typhoid fever and cholera-Escherichia coli-related diarrheas","volume":"46","author":"Clements","year":"1984","journal-title":"Infect Immunol"},{"issue":"1","key":"10.1016\/j.vaccine.2007.09.074_bib47","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1073\/pnas.93.1.226","article-title":"Potent immunogenicity of the B subunits of Escherichia coli heat-labile enterotoxin: receptor binding is essential and induces differential modulation of lymphocyte subsets","volume":"93","author":"Nashar","year":"1996","journal-title":"Proc Natl Acad Sci USA"},{"issue":"18","key":"10.1016\/j.vaccine.2007.09.074_bib48","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.1016\/j.vaccine.2007.01.043","article-title":"Transcutaneous immunization with the heat-labile toxin (LT) of enterotoxigenic Escherichia coli (ETEC): protective efficacy in a double-blind, placebo-controlled challenge study","volume":"25","author":"McKenzie","year":"2007","journal-title":"Vaccine"},{"issue":"22","key":"10.1016\/j.vaccine.2007.09.074_bib49","doi-asserted-by":"crossref","first-page":"4392","DOI":"10.1016\/j.vaccine.2007.03.034","article-title":"Randomised, double-blind, safety and efficacy of a killed oral vaccine for enterotoxigenic E. coli diarrhoea of travellers to Guatemala and Mexico","volume":"25","author":"Sack","year":"2007","journal-title":"Vaccine"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X07011127?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X07011127?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,14]],"date-time":"2023-05-14T18:47:45Z","timestamp":1684090065000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X07011127"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":49,"journal-issue":{"issue":"51","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["S0264410X07011127"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2007.09.074","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2007,12]]}}}