{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T13:20:56Z","timestamp":1789824056761,"version":"4.0.1"},"reference-count":41,"publisher":"Elsevier BV","issue":"1","license":[{"start":{"date-parts":[[2007,12,1]],"date-time":"2007-12-01T00:00:00Z","timestamp":1196467200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2007,12]]},"DOI":"10.1016\/j.vaccine.2007.10.052","type":"journal-article","created":{"date-parts":[[2007,12,5]],"date-time":"2007-12-05T23:31:01Z","timestamp":1196897461000},"page":"88-95","source":"Crossref","is-referenced-by-count":87,"title":["Recombinant Mycobacterium bovis BCG expressing the LipL32 antigen of Leptospira interrogans protects hamsters from challenge"],"prefix":"10.1016","volume":"26","author":[{"given":"Fabiana K\u00f6mmling","family":"Seixas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9verton Fagonde","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiane Drawanz","family":"Hartwig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Maia","family":"Cerqueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Amaral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel Quevedo","family":"Fagundes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robson Grando","family":"Dossa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odir Ant\u00f4nio","family":"Dellagostin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"5","key":"10.1016\/j.vaccine.2007.10.052_bib1","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1097\/01.qco.0000178824.05715.2c","article-title":"Leptospirosis","volume":"18","author":"McBride","year":"2005","journal-title":"Curr Opin Infect Dis"},{"issue":"5","key":"10.1016\/j.vaccine.2007.10.052_bib2","doi-asserted-by":"crossref","first-page":"1457","DOI":"10.1086\/314424","article-title":"Epidemic leptospirosis associated with pulmonary hemorrhage-Nicaragua, 1995","volume":"178","author":"Trevejo","year":"1998","journal-title":"J Infect Dis"},{"issue":"9181","key":"10.1016\/j.vaccine.2007.10.052_bib3","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1016\/S0140-6736(99)80012-9","article-title":"Urban epidemic of severe leptospirosis in Brazil. Salvador Leptospirosis Study Group","volume":"354","author":"Ko","year":"1999","journal-title":"Lancet"},{"issue":"6","key":"10.1016\/j.vaccine.2007.10.052_bib4","first-page":"194","article-title":"An urban outbreak of leptospirosis in Mumbai, India","volume":"55","author":"Bharadwaj","year":"2002","journal-title":"Jpn J Infect Dis"},{"issue":"4","key":"10.1016\/j.vaccine.2007.10.052_bib5","doi-asserted-by":"crossref","first-page":"309","DOI":"10.4103\/0255-0857.29404","article-title":"Leptospirosis: current status and future trends","volume":"24","author":"Hartskeerl","year":"2006","journal-title":"Indian J Med Microbiol"},{"issue":"11","key":"10.1016\/j.vaccine.2007.10.052_bib6","doi-asserted-by":"crossref","first-page":"6831","DOI":"10.1128\/IAI.69.11.6831-6838.2001","article-title":"Identification of the hemolysis-associated protein 1 as a cross-protective immunogen of Leptospira interrogans by adenovirus-mediated vaccination","volume":"69","author":"Branger","year":"2001","journal-title":"Infect Immun"},{"issue":"8","key":"10.1016\/j.vaccine.2007.10.052_bib7","doi-asserted-by":"crossref","first-page":"4853","DOI":"10.1128\/IAI.73.8.4853-4863.2005","article-title":"Surfaceome of Leptospira spp.","volume":"73","author":"Cullen","year":"2005","journal-title":"Infect Immun"},{"issue":"4","key":"10.1016\/j.vaccine.2007.10.052_bib8","doi-asserted-by":"crossref","first-page":"2276","DOI":"10.1128\/IAI.68.4.2276-2285.2000","article-title":"The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection","volume":"68","author":"Haake","year":"2000","journal-title":"Infect Immun"},{"issue":"9","key":"10.1016\/j.vaccine.2007.10.052_bib9","doi-asserted-by":"crossref","first-page":"2818","DOI":"10.1128\/JB.186.9.2818-2828.2004","article-title":"Molecular evolution and mosaicism of leptospiral outer membrane proteins involves horizontal DNA transfer","volume":"186","author":"Haake","year":"2004","journal-title":"J Bacteriol"},{"issue":"12","key":"10.1016\/j.vaccine.2007.10.052_bib10","doi-asserted-by":"crossref","first-page":"6572","DOI":"10.1128\/IAI.67.12.6572-6582.1999","article-title":"Leptospiral outer membrane proteins OmpL1 and LipL41 exhibit synergistic immunoprotection","volume":"67","author":"Haake","year":"1999","journal-title":"Infect Immun"},{"issue":"1","key":"10.1016\/j.vaccine.2007.10.052_bib11","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1128\/IAI.70.1.323-334.2002","article-title":"Novel 45-kilodalton leptospiral protein that is processed to a 31-kilodalton growth-phase-regulated peripheral membrane protein","volume":"70","author":"Matsunaga","year":"2002","journal-title":"Infect Immun"},{"issue":"11","key":"10.1016\/j.vaccine.2007.10.052_bib12","doi-asserted-by":"crossref","first-page":"5924","DOI":"10.1128\/IAI.70.11.5924-5930.2002","article-title":"Cloning and molecular characterization of an immunogenic LigA protein of Leptospira interrogans","volume":"70","author":"Palaniappan","year":"2002","journal-title":"Infect Immun"},{"issue":"1","key":"10.1016\/j.vaccine.2007.10.052_bib13","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1089\/hyb.2006.033","article-title":"Monoclonal antibodies against LipL32, the major outer membrane protein of pathogenic Leptospira: production, characterization, and testing in diagnostic applications","volume":"26","author":"Fernandes","year":"2007","journal-title":"Hybridoma"},{"issue":"8","key":"10.1016\/j.vaccine.2007.10.052_bib14","doi-asserted-by":"crossref","first-page":"2037","DOI":"10.1097\/01.ASN.0000022007.91733.62","article-title":"The Leptospira outer membrane protein LipL32 induces tubulointerstitial nephritis-mediated gene expression in mouse proximal tubule cells","volume":"13","author":"Yang","year":"2002","journal-title":"J Am Soc Nephrol"},{"issue":"4","key":"10.1016\/j.vaccine.2007.10.052_bib15","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1016\/j.bbrc.2005.06.045","article-title":"Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni: potentiation of hemolytic activity by LipL32","volume":"333","author":"Hauk","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"issue":"9","key":"10.1016\/j.vaccine.2007.10.052_bib16","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1128\/JCM.39.9.3303-3310.2001","article-title":"Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis","volume":"39","author":"Flannery","year":"2001","journal-title":"J Clin Microbiol"},{"issue":"7","key":"10.1016\/j.vaccine.2007.10.052_bib17","doi-asserted-by":"crossref","first-page":"4062","DOI":"10.1128\/IAI.73.7.4062-4069.2005","article-title":"Protection against Leptospira interrogans sensu lato challenge by DNA immunization with the gene encoding hemolysin-associated protein 1","volume":"73","author":"Branger","year":"2005","journal-title":"Infect Immun"},{"issue":"4\u20135","key":"10.1016\/j.vaccine.2007.10.052_bib18","doi-asserted-by":"crossref","first-page":"526","DOI":"10.1016\/S0171-2985(11)80459-6","article-title":"Experimental approaches to mechanisms of protection and pathogenesis in M. tuberculosis infection","volume":"191","author":"Bloom","year":"1994","journal-title":"Immunobiology"},{"issue":"30","key":"10.1016\/j.vaccine.2007.10.052_bib19","doi-asserted-by":"crossref","first-page":"4089","DOI":"10.1016\/S0264-410X(01)00155-4","article-title":"Recombinant BCG vaccines","volume":"19","author":"Ohara","year":"2001","journal-title":"Vaccine"},{"issue":"6326","key":"10.1016\/j.vaccine.2007.10.052_bib20","doi-asserted-by":"crossref","first-page":"456","DOI":"10.1038\/351456a0","article-title":"New use of BCG for recombinant vaccines","volume":"351","author":"Stover","year":"1991","journal-title":"Nature"},{"issue":"10","key":"10.1016\/j.vaccine.2007.10.052_bib21","doi-asserted-by":"crossref","first-page":"1209","DOI":"10.1016\/j.vaccine.2004.08.039","article-title":"Factors influencing the immune response to foreign antigen expressed in recombinant BCG vaccines","volume":"23","author":"Dennehy","year":"2005","journal-title":"Vaccine"},{"issue":"6506","key":"10.1016\/j.vaccine.2007.10.052_bib22","doi-asserted-by":"crossref","first-page":"552","DOI":"10.1038\/372552a0","article-title":"Systemic and mucosal immunity induced by BCG vector expressing outer-surface protein A of Borrelia burgdorferi","volume":"372","author":"Langermann","year":"1994","journal-title":"Nature"},{"issue":"24","key":"10.1016\/j.vaccine.2007.10.052_bib23","doi-asserted-by":"crossref","first-page":"11473","DOI":"10.1073\/pnas.90.24.11473","article-title":"Effective immunization against cutaneous leishmaniasis with recombinant bacille Calmette\u2013Guerin expressing the Leishmania surface proteinase gp63","volume":"90","author":"Connell","year":"1993","journal-title":"Proc Natl Acad Sci USA"},{"issue":"6","key":"10.1016\/j.vaccine.2007.10.052_bib24","doi-asserted-by":"crossref","first-page":"2277","DOI":"10.1084\/jem.180.6.2277","article-title":"Protective humoral response against pneumococcal infection in mice elicited by recombinant bacille Calmette\u2013Guerin vaccines expressing pneumococcal surface protein A","volume":"180","author":"Langermann","year":"1994","journal-title":"J Exp Med"},{"issue":"7\u20138","key":"10.1016\/j.vaccine.2007.10.052_bib25","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1016\/S0264-410X(00)00257-7","article-title":"Mycobacterium bovis bacillus Calmette\u2013Guerin induces protective immunity against infection by Plasmodium yoelii at blood-stage depending on shifting immunity toward Th1 type and inducing protective IgG2a after the parasite infection","volume":"19","author":"Matsumoto","year":"2000","journal-title":"Vaccine"},{"issue":"6","key":"10.1016\/j.vaccine.2007.10.052_bib26","doi-asserted-by":"crossref","first-page":"3336","DOI":"10.1128\/IAI.72.6.3336-3343.2004","article-title":"Recombinant Mycobacterium bovis BCG expressing the Sm14 antigen of Schistosoma mansoni protects mice from cercarial challenge","volume":"72","author":"Varaldo","year":"2004","journal-title":"Infect Immun"},{"issue":"1\u20132","key":"10.1016\/j.vaccine.2007.10.052_bib27","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.tube.2004.10.004","article-title":"A new vaccine against tuberculosis shows greater protection in a mouse model with progressive pulmonary tuberculosis","volume":"85","author":"Castanon-Arreola","year":"2005","journal-title":"Tuberculosis (Edinb)"},{"issue":"10","key":"10.1016\/j.vaccine.2007.10.052_bib28","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1016\/j.vaccine.2005.10.002","article-title":"A novel live recombinant mycobacterial vaccine against bovine tuberculosis more potent than BCG","volume":"24","author":"Horwitz","year":"2006","journal-title":"Vaccine"},{"issue":"4","key":"10.1016\/j.vaccine.2007.10.052_bib29","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1139\/w06-138","article-title":"Evaluation of different ways of presenting LipL32 to the immune system with the aim of developing a recombinant vaccine against leptospirosis","volume":"53","author":"Seixas","year":"2007","journal-title":"Can J Microbiol"},{"key":"10.1016\/j.vaccine.2007.10.052_bib30","first-page":"493","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed","year":"1938","journal-title":"Am J Hyg"},{"issue":"15\u201316","key":"10.1016\/j.vaccine.2007.10.052_bib31","doi-asserted-by":"crossref","first-page":"1906","DOI":"10.1016\/S0264-410X(00)00439-4","article-title":"Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette\u2013Guerin over-producing three putative protective antigen candidates","volume":"19","author":"Ohara","year":"2001","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.vaccine.2007.10.052_bib32","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1084\/jem.178.1.197","article-title":"Protective immunity elicited by recombinant bacille Calmette\u2013Guerin (BCG) expressing outer surface protein A (OspA) lipoprotein: a candidate Lyme disease vaccine","volume":"178","author":"Stover","year":"1993","journal-title":"J Exp Med"},{"issue":"1\u20132","key":"10.1016\/j.vaccine.2007.10.052_bib33","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0264-410X(02)00443-7","article-title":"Construction and immunogenicity of recombinant Mycobacterium bovis BCG expressing GP5 and M protein of porcine reproductive respiratory syndrome virus","volume":"21","author":"Bastos","year":"2002","journal-title":"Vaccine"},{"issue":"22","key":"10.1016\/j.vaccine.2007.10.052_bib34","doi-asserted-by":"crossref","first-page":"3331","DOI":"10.1111\/j.1365-2958.1992.tb02201.x","article-title":"Expression of Escherichia coli beta-galactosidase in Mycobacterium bovis BCG using an expression system isolated from Mycobacterium paratuberculosis which induced humoral and cellular immune responses","volume":"6","author":"Murray","year":"1992","journal-title":"Mol Microbiol"},{"issue":"Pt 8","key":"10.1016\/j.vaccine.2007.10.052_bib35","doi-asserted-by":"crossref","first-page":"1785","DOI":"10.1099\/13500872-141-8-1785","article-title":"Activity of mycobacterial promoters during intracellular and extracellular growth","volume":"141","author":"Dellagostin","year":"1995","journal-title":"Microbiology"},{"issue":"6","key":"10.1016\/j.vaccine.2007.10.052_bib36","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1054\/tube.2002.0374","article-title":"Expression of foreign genes in Mycobacterium bovis BCG strains using different promoters reveals instability of the hsp60 promoter for expression of foreign genes in Mycobacterium bovis BCG strains","volume":"82","author":"Al Zarouni","year":"2002","journal-title":"Tuberculosis (Edinb)"},{"issue":"Pt 7","key":"10.1016\/j.vaccine.2007.10.052_bib37","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1099\/00221287-148-7-1999","article-title":"Comparative evaluation of Mycobacterium vaccae as a surrogate cloning host for use in the study of mycobacterial genetics","volume":"148","author":"Medeiros","year":"2002","journal-title":"Microbiology"},{"issue":"37\u201339","key":"10.1016\/j.vaccine.2007.10.052_bib38","doi-asserted-by":"crossref","first-page":"6332","DOI":"10.1016\/j.vaccine.2006.05.028","article-title":"Immunogenicity of Mycobacterium bovis BCG expressing Anaplasma marginale MSP1a antigen","volume":"24","author":"Michelon","year":"2006","journal-title":"Vaccine"},{"issue":"11\u201312","key":"10.1016\/j.vaccine.2007.10.052_bib39","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/j.vaccine.2003.10.007","article-title":"Leptospiral immunoglobulin-like proteins elicit protective immunity","volume":"22","author":"Koizumi","year":"2004","journal-title":"Vaccine"},{"issue":"3","key":"10.1016\/j.vaccine.2007.10.052_bib40","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1128\/IAI.74.3.1745-1750.2006","article-title":"Immunoprotection of recombinant leptospiral immunoglobulin-like protein A against Leptospira interrogans serovar Pomona infection","volume":"74","author":"Palaniappan","year":"2006","journal-title":"Infect Immun"},{"issue":"33","key":"10.1016\/j.vaccine.2007.10.052_bib41","doi-asserted-by":"crossref","first-page":"6277","DOI":"10.1016\/j.vaccine.2007.05.053","article-title":"The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis","volume":"25","author":"Silva","year":"2007","journal-title":"Vaccine"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X07012212?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X07012212?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,4,29]],"date-time":"2020-04-29T05:09:17Z","timestamp":1588136957000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X07012212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":41,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["S0264410X07012212"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2007.10.052","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2007,12]]}}}