{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:44:24Z","timestamp":1761662664658},"reference-count":50,"publisher":"Elsevier BV","issue":"38","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2008,9]]},"DOI":"10.1016\/j.vaccine.2008.07.030","type":"journal-article","created":{"date-parts":[[2008,8,4]],"date-time":"2008-08-04T13:45:30Z","timestamp":1217857530000},"page":"4913-4917","source":"Crossref","is-referenced-by-count":22,"title":["Evaluation of Brucella melitensis B115 as rough-phenotype vaccine against B. melitensis and B. ovis infections"],"prefix":"10.1016","volume":"26","author":[{"given":"R.","family":"Adone","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Francia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Ciuchini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2008.07.030_bib1","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1128\/CDLI.6.6.787-790.1999","article-title":"Complement fixation test to assess humoral immunity in cattle and sheep vaccinated with Brucella abortus RB51","volume":"6","author":"Adone","year":"1999","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1128\/CDLI.8.2.385-387.2001","article-title":"Field validation of RB51 antigen in a complement fixation test to identify calves vaccinated with Brucella abortus RB51","volume":"8","author":"Adone","year":"2001","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib3","doi-asserted-by":"crossref","first-page":"4198","DOI":"10.1128\/IAI.73.7.4198-4204.2005","article-title":"Protective properties of rifampin-resistant rough mutants of Brucella melitensis","volume":"73","author":"Adone","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib4","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1111\/j.1365-2672.2008.03787.x","article-title":"Brucella melitensis B115-based complement fixation test to detect antibodies induced by Brucella rough strains","volume":"105","author":"Adone","year":"2008","journal-title":"J Appl Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib5","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/IAI.66.3.1008-1016.1998","article-title":"Transposon-derived Brucella abortus rough mutants are attenuated and exhibit reduced intracellular survival","volume":"66","author":"Allen","year":"1998","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib6","first-page":"793","article-title":"Rev. 1 Brucella melitensis vaccine: a review of ten years of study","volume":"371","author":"Alton","year":"1967","journal-title":"Vet Bull"},{"key":"10.1016\/j.vaccine.2008.07.030_bib7","series-title":"Techniques for the brucellosis laboratory","author":"Alton","year":"1988"},{"key":"10.1016\/j.vaccine.2008.07.030_bib8","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/S0378-1135(02)00231-6","article-title":"Control of small ruminant brucellosis by use of Brucella melitensis Rev. 1 vaccine: laboratory aspects and field observations","volume":"90","author":"Banai","year":"2002","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib9","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/0140-6736(93)91571-3","article-title":"Brucella melitensis Rev. 1 as cause of human brucellosis","volume":"342","author":"Blasco","year":"1993","journal-title":"Lancet"},{"key":"10.1016\/j.vaccine.2008.07.030_bib10","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0167-5877(96)01110-5","article-title":"A review of the use of Brucella melitensis Rev. 1 vaccine in adult sheep and goats","volume":"31","author":"Blasco","year":"1997","journal-title":"Prev Vet Med"},{"key":"10.1016\/j.vaccine.2008.07.030_bib11","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S1045-1056(05)80025-9","article-title":"Brucella melitensis Rev. 1 living attenuated vaccine: stability of markers, residual virulence and immunogenicity in mice","volume":"19","author":"Bosseray","year":"1991","journal-title":"Biologicals"},{"key":"10.1016\/j.vaccine.2008.07.030_bib12","doi-asserted-by":"crossref","first-page":"2660","DOI":"10.1128\/JCM.32.11.2660-2666.1994","article-title":"Differentiation of Brucella abortus bv. 1, 2, and 4; Brucella melitensis; Brucella ovis; and Brucella suis bv. 1 by PCR","volume":"32","author":"Bricker","year":"1994","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib13","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.2460\/ajvr.1993.54.10.1591","article-title":"Immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of Brucella abortus","volume":"54","author":"Cheville","year":"1993","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.vaccine.2008.07.030_bib14","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1128\/IAI.60.1.312-315.1992","article-title":"Protection against Brucella melitensis or Brucella abortus in mice with immunoglobulin G (IgG), IgA and IgM monoclonal antibodies specific for a common epitope shared by the Brucella A and M smooth lipopolysaccharide","volume":"60","author":"Cloeckaert","year":"1992","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib15","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1099\/00221287-138-6-1211","article-title":"O-chain expression in the rough Brucella melitensis strain B115: induction of O-polysaccharide-specific monoclonal antibodies and intracellular localization demonstrated by immunoelectron microscopy","volume":"138","author":"Cloeckaert","year":"1992","journal-title":"J Gen Microb"},{"key":"10.1016\/j.vaccine.2008.07.030_bib16","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1099\/00221287-139-7-1551","article-title":"Characterization of O-polysaccharide specific monoclonal antibodies derived from mice infected with the rough Brucella melitensis strain B115","volume":"139","author":"Cloeckaert","year":"1993","journal-title":"J Gen Microb"},{"key":"10.1016\/j.vaccine.2008.07.030_bib17","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1073\/pnas.221575398","article-title":"The genome sequence of the facultative intracellular pathogen Brucella melitensis","volume":"99","author":"DelVecchio","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2008.07.030_bib18","doi-asserted-by":"crossref","first-page":"741","DOI":"10.20506\/rst.20.3.1305","article-title":"Comparison of the efficacy of Brucella abortus strain RB51 and Brucella melitensis Rev. 1 live vaccines against experimental infection with Brucella melitensis in pregnant ewes","volume":"20","author":"El Idrissi","year":"2001","journal-title":"Rev Sci Tech Off Int Epiz"},{"key":"10.1016\/j.vaccine.2008.07.030_bib19","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0034-5288(98)90135-7","article-title":"Evaluation of a rough mutant of Brucella melitensis in pregnant goats","volume":"64","author":"Elzer","year":"1998","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.vaccine.2008.07.030_bib20","first-page":"255","article-title":"Brucella melitensis infection in sheep: present and future","volume":"29","author":"Garin-Bastuji","year":"1998","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2008.07.030_bib21","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/S0923-2508(00)90130-X","article-title":"Genetic organisation of the lipopolysaccharide O-antigen biosynthesis region of Brucella melitensis 16M (wbk)","volume":"151","author":"Godfroid","year":"2000","journal-title":"Res Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib22","series-title":"Brucellosis international research conference","article-title":"Comparison of Brucella melitensis R mutants with intact or defective lipopolysaccharide core as vaccines in BALB\/c mice","author":"Gonzalez","year":"2003"},{"key":"10.1016\/j.vaccine.2008.07.030_bib23","series-title":"Brucellosis international research conference","article-title":"Virulence and vaccine efficacy in mice of lipopolysaccharide mutants obtained from Brucella melitensis strains 16M and H38","author":"Grillo","year":"2003"},{"key":"10.1016\/j.vaccine.2008.07.030_bib24","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1006\/biol.2000.0249","article-title":"In vitro markers and biological activity in mice of seed lot strains and commercial Brucella melitensis Rev1 and Brucella abortus B19 vaccines","volume":"28","author":"Grill\u00f3","year":"2000","journal-title":"Biologicals"},{"key":"10.1016\/j.vaccine.2008.07.030_bib25","first-page":"205","article-title":"Responses of ewes to B. melitensis Rev. 1 vaccine administered by subcutaneous or conjunctival routes at different stages of pregnancy","volume":"20","author":"Jim\u00e9nez de Bag\u00fc\u00e9s","year":"1989","journal-title":"Ann Rech Vet"},{"key":"10.1016\/j.vaccine.2008.07.030_bib26","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/0378-1135(92)90117-C","article-title":"An ELISA with Brucella lipopolysaccharide antigen for the diagnosis of B. melitensis infection in sheep and for the evaluation of serological responses following subcutaneous or conjunctival B. melitensis strain Rev. 1","volume":"30","author":"Jim\u00e9nez de Bag\u00fc\u00e9s","year":"1992","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib27","doi-asserted-by":"crossref","first-page":"4990","DOI":"10.1128\/IAI.62.11.4990-4996.1994","article-title":"Vaccination with Brucella abortus rough mutant RB51 protects BALB\/c mice against virulent strains of Brucella abortus, Brucella melitensis, and Brucella ovis","volume":"62","author":"Jim\u00e9nez de Bag\u00fc\u00e9s","year":"1994","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib28","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/0264-410X(95)93317-3","article-title":"The Brucella abortus RB51 vaccine does not confer protection against Brucella ovis in rams","volume":"13","author":"Jim\u00e9nez de Bag\u00fc\u00e9s","year":"1995","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2008.07.030_bib29","doi-asserted-by":"crossref","first-page":"3830","DOI":"10.1128\/IAI.67.8.3830-3835.1999","article-title":"Genetic characterization of a Tn5-disrupted glycosyltransferase gene homologue in Brucella abortus and its effect on lipopolysaccharide composition and virulence","volume":"67","author":"McQuiston","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib30","first-page":"902","article-title":"Characterization of Brucella abortus strain 19 isolated from human and bovine tissues and fluids","volume":"46","author":"Meyer","year":"1985","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.vaccine.2008.07.030_bib31","doi-asserted-by":"crossref","first-page":"3261","DOI":"10.1128\/IAI.71.6.3261-3271.2003","article-title":"Characterization of Brucella abortus O-polysaccharide and core lipopolysaccharide mutants and demonstration that a complete core is required for rough vaccines to be efficient against Brucella abortus and Brucella ovis in the mouse model","volume":"71","author":"Monreal","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib32","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1128\/IAI.53.2.245-251.1986","article-title":"Comparison of living and nonliving vaccines for Brucella abortus in BALB\/c mice","volume":"53","author":"Montaraz","year":"1986","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1051\/vetres:2003037","article-title":"Rough vaccines in animal brucellosis: structural and genetic basis and present status","volume":"35","author":"Moriyon","year":"2004","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2008.07.030_bib34","unstructured":"National Academy of Sciences. Brucellosis research: an evaluation. Report of the subcommittee on brucellosis research, National Academy of Sciences. Washington, DC: National Academy Press; 1977. p. 61\u201377."},{"key":"10.1016\/j.vaccine.2008.07.030_bib35","series-title":"Animal brucellosis","first-page":"284","article-title":"Vaccination","author":"Nicoletti","year":"1990"},{"key":"10.1016\/j.vaccine.2008.07.030_bib36","series-title":"Animal brucellosis","first-page":"83","article-title":"Response to Brucella abortus","author":"Nicoletti","year":"1990"},{"key":"10.1016\/j.vaccine.2008.07.030_bib37","unstructured":"Office International des Epizooties. Manual of diagnostic tests and vaccines for terrestrial animals, 5th ed. Paris, France: Office International des Epizooties; 2004."},{"key":"10.1016\/j.vaccine.2008.07.030_bib38","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1053\/rvsc.1998.0251","article-title":"Responses of cattle to two dosages of Brucella abortus strain RB51: serology, clearance and efficacy","volume":"66","author":"Olsen","year":"1999","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.vaccine.2008.07.030_bib39","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1128\/JCM.25.11.2090-2093.1987","article-title":"Rapid identification of smooth Brucella species with a monoclonal antibody","volume":"25","author":"Roop","year":"1987","journal-title":"J Clin Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib40","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0378-1135(91)90091-S","article-title":"Biological properties of RB51; a stable rough strain of Brucella abortus","volume":"28","author":"Schurig","year":"1991","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib41","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0378-1135(02)00255-9","article-title":"Brucellosis vaccines: past, present and future","volume":"90","author":"Schurig","year":"2002","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib42","doi-asserted-by":"crossref","first-page":"209","DOI":"10.3109\/10408419009105726","article-title":"Pathogenesis of Brucella","volume":"17","author":"Smith","year":"1990","journal-title":"Crit Rev Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib43","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1128\/IAI.64.3.1030-1034.1996","article-title":"Antibody responses to Brucella abortus 2308 in cattle vaccinated with B. abortus RB51","volume":"64","author":"Stevens","year":"1996","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib44","doi-asserted-by":"crossref","first-page":"6264","DOI":"10.1128\/IAI.71.11.6264-6269.2003","article-title":"Evaluation of Brucella abortus phosphoglucomutase (pgm) mutant as a new live rough-phenotype vaccine","volume":"71","author":"Ugalde","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib45","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1128\/CDLI.6.5.760-764.1999","article-title":"Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains","volume":"6","author":"Vemulapalli","year":"1999","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib46","doi-asserted-by":"crossref","first-page":"3286","DOI":"10.1128\/IAI.68.6.3286-3289.2000","article-title":"Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51","volume":"68","author":"Vemulapalli","year":"2000","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib47","doi-asserted-by":"crossref","first-page":"3927","DOI":"10.1128\/IAI.68.7.3927-3932.2000","article-title":"Complementation of Brucella abortus RB51 with a functional wboA gene results in O-antigen synthesis and enhanced vaccine efficacy but no change in rough phenotype and attenuation","volume":"68","author":"Vemulapalli","year":"2000","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2008.07.030_bib48","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.vetmic.2004.07.001","article-title":"Enhanced efficacy of recombinant Brucella abortus RB51 vaccines against B. melitensis infection in mice","volume":"102","author":"Vemulapalli","year":"2004","journal-title":"Vet Microb"},{"key":"10.1016\/j.vaccine.2008.07.030_bib49","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/0923-2508(92)90098-9","article-title":"A rapid and sensitive method for the identification of Brucella species with a monoclonal antibody","volume":"143","author":"Vizcaino","year":"1992","journal-title":"Res Microbiol"},{"key":"10.1016\/j.vaccine.2008.07.030_bib50","doi-asserted-by":"crossref","first-page":"677","DOI":"10.2460\/ajvr.1996.57.05.677","article-title":"Protection of BALB\/c mice against homologous and heterologous species of Brucella by rough strain vaccines derived from Brucella melitensis and Brucella suis biovar 4","volume":"57","author":"Winter","year":"1996","journal-title":"Am J Vet Res"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X08009742?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X08009742?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,2,28]],"date-time":"2024-02-28T23:37:36Z","timestamp":1709163456000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X08009742"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,9]]},"references-count":50,"journal-issue":{"issue":"38","published-print":{"date-parts":[[2008,9]]}},"alternative-id":["S0264410X08009742"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2008.07.030","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2008,9]]}}}