{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T20:17:04Z","timestamp":1789503424354,"version":"build-2803163510"},"reference-count":42,"publisher":"Elsevier BV","issue":"20","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2010,4]]},"DOI":"10.1016\/j.vaccine.2010.02.094","type":"journal-article","created":{"date-parts":[[2010,3,12]],"date-time":"2010-03-12T12:52:47Z","timestamp":1268398367000},"page":"3571-3581","source":"Crossref","is-referenced-by-count":173,"title":["Ag85B\u2013ESAT-6 adjuvanted with IC31\u00ae promotes strong and long-lived Mycobacterium tuberculosis specific T cell responses in na\u00efve human volunteers"],"prefix":"10.1016","volume":"28","author":[{"given":"Jaap T.","family":"van Dissel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra M.","family":"Arend","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corine","family":"Prins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Bang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pernille Nyholm","family":"Tingskov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Lingnau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Nouta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mich\u00e8l R.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ida","family":"Rosenkrands","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom H.M.","family":"Ottenhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Kromann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. Mark","family":"Doherty","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Andersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"May (5)","key":"10.1016\/j.vaccine.2010.02.094_bib1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.2471\/BLT.06.037820","article-title":"Planning to improve global health: the next decade of tuberculosis control","volume":"85","author":"Maher","year":"2007","journal-title":"Bull World Health Organ"},{"issue":"May (17)","key":"10.1016\/j.vaccine.2010.02.094_bib2","doi-asserted-by":"crossref","first-page":"2086","DOI":"10.1001\/jama.291.17.2086","article-title":"Long-term efficacy of BCG vaccine in American Indians and Alaska Natives: a 60-year follow-up study","volume":"291","author":"Aronson","year":"2004","journal-title":"JAMA"},{"issue":"December (12)","key":"10.1016\/j.vaccine.2010.02.094_bib3","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1097\/01.inf.0000243765.33880.54","article-title":"The impact of a change in bacille Calmette\u2013Guerin vaccine policy on tuberculosis incidence in children in Cape Town","volume":"25","author":"Mahomed","year":"2006","journal-title":"South Africa Pediatr Infect Dis J"},{"issue":"March (3)","key":"10.1016\/j.vaccine.2010.02.094_bib4","first-page":"200","article-title":"Does the efficacy of BCG decline with time since vaccination?","volume":"2","author":"Sterne","year":"1998","journal-title":"Int J Tuberc Lung Dis"},{"issue":"July (7)","key":"10.1016\/j.vaccine.2010.02.094_bib5","doi-asserted-by":"crossref","first-page":"505","DOI":"10.2471\/BLT.08.055657","article-title":"Disseminated bacille Calmette\u2013Guerin disease in HIV-infected South African infants","volume":"87","author":"Hesseling","year":"2009","journal-title":"Bull World Health Organ"},{"issue":"January (1)","key":"10.1016\/j.vaccine.2010.02.094_bib6","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.vaccine.2006.07.020","article-title":"The risk of disseminated Bacille Calmette\u2013Guerin (BCG) disease in HIV-infected children","volume":"25","author":"Hesseling","year":"2007","journal-title":"Vaccine"},{"issue":"December (12)","key":"10.1016\/j.vaccine.2010.02.094_bib7","first-page":"1376","article-title":"Consensus statement on the revised World Health Organization recommendations for BCG vaccination in HIV-infected infants","volume":"12","author":"Hesseling","year":"2008","journal-title":"Int J Tuberc Lung Dis"},{"issue":"June (6)","key":"10.1016\/j.vaccine.2010.02.094_bib8","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1038\/nrmicro1419","article-title":"Advances in tuberculosis vaccine strategies","volume":"4","author":"Skeiky","year":"2006","journal-title":"Nat Rev Microbiol"},{"issue":"October (10)","key":"10.1016\/j.vaccine.2010.02.094_bib9","doi-asserted-by":"crossref","first-page":"1539","DOI":"10.1517\/14712598.7.10.1539","article-title":"Tuberculosis subunit vaccines: from basic science to clinical testing","volume":"7","author":"Doherty","year":"2007","journal-title":"Expert Opin Biol Ther"},{"issue":"September (9)","key":"10.1016\/j.vaccine.2010.02.094_bib10","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1038\/nri1920","article-title":"Envisioning future strategies for vaccination against tuberculosis","volume":"6","author":"Kaufmann","year":"2006","journal-title":"Nat Rev Immunol"},{"issue":"June (3)","key":"10.1016\/j.vaccine.2010.02.094_bib11","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1586\/14760584.3.3.299","article-title":"Developing an improved vaccine against tuberculosis","volume":"3","author":"McShane","year":"2004","journal-title":"Expert Rev Vaccines"},{"issue":"April (21)","key":"10.1016\/j.vaccine.2010.02.094_bib12","doi-asserted-by":"crossref","first-page":"2740","DOI":"10.1016\/j.vaccine.2004.11.051","article-title":"Protection of macaques against Mycobacterium tuberculosis infection by a subunit vaccine based on a fusion protein of antigen 85B and ESAT-6","volume":"23","author":"Langermans","year":"2005","journal-title":"Vaccine"},{"issue":"January\u2013March (1\u20132)","key":"10.1016\/j.vaccine.2010.02.094_bib13","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.tube.2004.09.009","article-title":"Evaluation of vaccines in the EU TB vaccine cluster using a guinea pig aerosol infection model of tuberculosis","volume":"85","author":"Williams","year":"2005","journal-title":"Tuberculosis (Edinb)"},{"issue":"October (10)","key":"10.1016\/j.vaccine.2010.02.094_bib14","doi-asserted-by":"crossref","first-page":"6148","DOI":"10.1128\/IAI.72.10.6148-6150.2004","article-title":"Protective effect of a tuberculosis subunit vaccine based on a fusion of antigen 85B and ESAT-6 in the aerosol guinea pig model","volume":"72","author":"Olsen","year":"2004","journal-title":"Infect Immun"},{"issue":"December (12)","key":"10.1016\/j.vaccine.2010.02.094_bib15","doi-asserted-by":"crossref","first-page":"2146","DOI":"10.1086\/425931","article-title":"Comparative analysis of different vaccine constructs expressing defined antigens from Mycobacterium tuberculosis","volume":"190","author":"Doherty","year":"2004","journal-title":"J Infect Dis"},{"issue":"October","key":"10.1016\/j.vaccine.2010.02.094_bib16","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1111\/j.1600-065X.2007.00545.x","article-title":"Mycobacterium tuberculosis, macrophages, and the innate immune response: does common variation matter?","volume":"219","author":"Berrington","year":"2007","journal-title":"Immunol Rev"},{"issue":"October","key":"10.1016\/j.vaccine.2010.02.094_bib17","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1111\/j.1600-065X.2007.00547.x","article-title":"Mycobacterium tuberculosis and the environment within the phagosome","volume":"219","author":"Rohde","year":"2007","journal-title":"Immunol Rev"},{"issue":"December (12)","key":"10.1016\/j.vaccine.2010.02.094_bib18","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1016\/S1473-3099(04)01203-4","article-title":"Human genetics of intracellular infectious diseases: molecular and cellular immunity against mycobacteria and salmonellae","volume":"4","author":"van de Vosse","year":"2004","journal-title":"Lancet Infect Dis"},{"issue":"March (17\u201319)","key":"10.1016\/j.vaccine.2010.02.094_bib19","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.1016\/S0264-410X(00)00519-3","article-title":"Tuberculosis subunit vaccine development: on the role of interferon-gamma","volume":"19","author":"Agger","year":"2001","journal-title":"Vaccine"},{"issue":"August (8)","key":"10.1016\/j.vaccine.2010.02.094_bib20","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1016\/S1473-3099(06)70552-7","article-title":"Early clinical trials with a new tuberculosis vaccine, MVA85A, in tuberculosis-endemic countries: issues in study design","volume":"6","author":"Ibanga","year":"2006","journal-title":"Lancet Infect Dis"},{"issue":"June (2)","key":"10.1016\/j.vaccine.2010.02.094_bib21","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1111\/j.1365-2567.2007.02751.x","article-title":"Adjuvant modulation of the cytokine balance in Mycobacterium tuberculosis subunit vaccines; immunity, pathology and protection","volume":"124","author":"Agger","year":"2008","journal-title":"Immunology"},{"issue":"October (7)","key":"10.1016\/j.vaccine.2010.02.094_bib22","doi-asserted-by":"crossref","first-page":"4403","DOI":"10.4049\/jimmunol.0900164","article-title":"Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity","author":"McKee","year":"2009","journal-title":"J Immunol"},{"issue":"October (5)","key":"10.1016\/j.vaccine.2010.02.094_bib23","doi-asserted-by":"crossref","first-page":"488","DOI":"10.1111\/j.0818-9641.2004.01272.x","article-title":"Vaccine adjuvants: current state and future trends","volume":"82","author":"Petrovsky","year":"2004","journal-title":"Immunol Cell Biol"},{"issue":"May (5)","key":"10.1016\/j.vaccine.2010.02.094_bib24","doi-asserted-by":"crossref","first-page":"1247","DOI":"10.1002\/eji.200737889","article-title":"Protective anti-mycobacterial T cell responses through exquisite in vivo activation of vaccine-targeted dendritic cells","volume":"38","author":"Kamath","year":"2008","journal-title":"Eur J Immunol"},{"issue":"October (5)","key":"10.1016\/j.vaccine.2010.02.094_bib25","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1586\/14760584.6.5.741","article-title":"IC31 and IC30, novel types of vaccine adjuvant based on peptide delivery systems","volume":"6","author":"Lingnau","year":"2007","journal-title":"Expert Rev Vaccines"},{"issue":"June (26)","key":"10.1016\/j.vaccine.2010.02.094_bib26","doi-asserted-by":"crossref","first-page":"5452","DOI":"10.1016\/j.vaccine.2006.03.072","article-title":"Protective immunity to tuberculosis with Ag85B\u2013ESAT-6 in a synthetic cationic adjuvant system IC31","volume":"24","author":"Agger","year":"2006","journal-title":"Vaccine"},{"issue":"11","key":"10.1016\/j.vaccine.2010.02.094_bib27","doi-asserted-by":"crossref","first-page":"e3683","DOI":"10.1371\/journal.pone.0003683","article-title":"Adult-like anti-mycobacterial T cell and in vivo dendritic cell responses following neonatal immunization with Ag85B\u2013ESAT-6 in the IC31 adjuvant","volume":"3","author":"Kamath","year":"2008","journal-title":"PLoS One"},{"issue":"April (4)","key":"10.1016\/j.vaccine.2010.02.094_bib28","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/S0196-0644(95)70270-9","article-title":"Effects of physical activity on creatine phosphokinase and the isoenzyme creatine kinase-MB","volume":"25","author":"Schneider","year":"1995","journal-title":"Ann Emerg Med"},{"issue":"May (5\u20136)","key":"10.1016\/j.vaccine.2010.02.094_bib29","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1016\/j.micinf.2005.03.013","article-title":"TB subunit vaccines\u2014putting the pieces together","volume":"7","author":"Andersen","year":"2005","journal-title":"Microbes Infect"},{"issue":"June (26)","key":"10.1016\/j.vaccine.2010.02.094_bib30","doi-asserted-by":"crossref","first-page":"5461","DOI":"10.1016\/j.vaccine.2006.03.071","article-title":"IC31, a novel adjuvant signaling via TLR9, induces potent cellular and humoral immune responses","volume":"24","author":"Schellack","year":"2006","journal-title":"Vaccine"},{"issue":"July (7)","key":"10.1016\/j.vaccine.2010.02.094_bib31","doi-asserted-by":"crossref","first-page":"475","DOI":"10.4161\/hv.8570","article-title":"The candidate tuberculosis vaccine Mtb72F\/AS02A: Tolerability and immunogenicity in humans","volume":"5","author":"Von Eschen","year":"2009","journal-title":"Hum Vaccin"},{"issue":"June (6)","key":"10.1016\/j.vaccine.2010.02.094_bib32","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1111\/j.1462-5822.2007.00892.x","article-title":"The ESAT6 protein of Mycobacterium tuberculosis induces apoptosis of macrophages by activating caspase expression","volume":"9","author":"Derrick","year":"2007","journal-title":"Cell Microbiol"},{"issue":"December (12)","key":"10.1016\/j.vaccine.2010.02.094_bib33","doi-asserted-by":"crossref","first-page":"5478","DOI":"10.1128\/IAI.00614-08","article-title":"Evidence for pore formation in host cell membranes by ESX-1-secreted ESAT-6 and its role in Mycobacterium marinum escape from the vacuole","volume":"76","author":"Smith","year":"2008","journal-title":"Infect Immun"},{"issue":"May-June (4)","key":"10.1016\/j.vaccine.2010.02.094_bib34","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/icb.2009.14","article-title":"TB vaccines: current status and future perspectives","volume":"87","author":"Aagaard","year":"2009","journal-title":"Immunol Cell Biol"},{"issue":"November (11)","key":"10.1016\/j.vaccine.2010.02.094_bib35","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1038\/nm1128","article-title":"Recombinant modified vaccinia virus Ankara expressing antigen 85A boosts BCG-primed and naturally acquired antimycobacterial immunity in humans","volume":"10","author":"McShane","year":"2004","journal-title":"Nat Med"},{"issue":"11","key":"10.1016\/j.vaccine.2010.02.094_bib36","doi-asserted-by":"crossref","first-page":"e7972","DOI":"10.1371\/journal.pone.0007972","article-title":"Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from Mycobacterium tuberculosis","volume":"4","author":"Smith","year":"2009","journal-title":"PLoS One"},{"issue":"August (8)","key":"10.1016\/j.vaccine.2010.02.094_bib37","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1038\/nrmicro1211","article-title":"The success and failure of BCG\u2014implications for a novel tuberculosis vaccine","volume":"3","author":"Andersen","year":"2005","journal-title":"Nat Rev Microbiol"},{"issue":"July (1 Pt 1)","key":"10.1016\/j.vaccine.2010.02.094_bib38","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1542\/peds.96.1.29","article-title":"The efficacy of bacillus Calmette\u2013Guerin vaccination of newborns and infants in the prevention of tuberculosis: meta-analyses of the published literature","volume":"96","author":"Colditz","year":"1995","journal-title":"Pediatrics"},{"key":"10.1016\/j.vaccine.2010.02.094_bib39","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1186\/1471-2334-8-9","article-title":"Persistence of the immune response induced by BCG vaccination","volume":"8","author":"Weir","year":"2008","journal-title":"BMC Infect Dis"},{"issue":"August (4)","key":"10.1016\/j.vaccine.2010.02.094_bib40","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1016\/j.coi.2006.05.016","article-title":"Progress in tuberculosis vaccine development","volume":"18","author":"Baumann","year":"2006","journal-title":"Curr Opin Immunol"},{"issue":"Aug (6797)","key":"10.1016\/j.vaccine.2010.02.094_bib41","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1038\/35021239","article-title":"Vaccines against intracellular infections requiring cellular immunity","volume":"406","author":"Seder","year":"2000","journal-title":"Nature"},{"issue":"June (12)","key":"10.1016\/j.vaccine.2010.02.094_bib42","doi-asserted-by":"crossref","first-page":"8047","DOI":"10.4049\/jimmunol.0801592","article-title":"Tuberculosis subunit vaccination provides long-term protective immunity characterized by multifunctional CD4 memory T cells","volume":"182","author":"Lindenstrom","year":"2009","journal-title":"J Immunol"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X10002902?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X10002902?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T23:42:40Z","timestamp":1685490160000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X10002902"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":42,"journal-issue":{"issue":"20","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["S0264410X10002902"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2010.02.094","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2010,4]]}}}