{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T04:45:12Z","timestamp":1766551512598,"version":"build-2065373602"},"reference-count":18,"publisher":"Elsevier BV","issue":"34","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2011,6,19]],"date-time":"2011-06-19T00:00:00Z","timestamp":1308441600000},"content-version":"vor","delay-in-days":322,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2010,8]]},"DOI":"10.1016\/j.vaccine.2010.05.079","type":"journal-article","created":{"date-parts":[[2010,6,20]],"date-time":"2010-06-20T20:27:35Z","timestamp":1277065655000},"page":"5648-5651","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Determination of hydrazine in a meningococcal C conjugate vaccine intermediary product"],"prefix":"10.1016","volume":"28","author":[{"given":"Renata Chagas","family":"Bastos","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juliana Machado","family":"de Carvalho","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivna Alana Freitas Brasileiro","family":"da Silveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvana","family":"do Couto Jacob","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katia Christina","family":"Leandro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2010.05.079_bib1","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1038\/nbt1261","article-title":"Vaccine manufacturing: challenges and solutions","volume":"24","author":"Ulmer","year":"2006","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.vaccine.2010.05.079_bib2","doi-asserted-by":"crossref","first-page":"7261","DOI":"10.1016\/j.vaccine.2007.07.037","article-title":"Characterization and immunogenicity of meningococcal group C conjugate vaccine prepared using hydrazine-activated tetanus toxoid","volume":"25","author":"Silveira","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2010.05.079_bib3","series-title":"Guia de Vigil\u00e2ncia Epidemiol\u00f3gica","article-title":"Minist\u00e9rio da Sa\u00fade. Secretaria de Vigil\u00e2ncia em Sa\u00fade. Meningites","author":"BRASIL","year":"2005"},{"key":"10.1016\/j.vaccine.2010.05.079_bib4","doi-asserted-by":"crossref","first-page":"S35","DOI":"10.2223\/JPED.1495","article-title":"Meningococcal conjugate vaccines: efficacy and new combinations","volume":"82","author":"S\u00e1fadi","year":"2006","journal-title":"J Pediatr"},{"key":"10.1016\/j.vaccine.2010.05.079_bib5","doi-asserted-by":"crossref","first-page":"5903","DOI":"10.1073\/pnas.0710799105","article-title":"Rational chemical design of the carbohydrate in a glycoconjugate vaccine enhances IgM-to-IgG switching","volume":"105","author":"Guttormsen","year":"2008","journal-title":"PNAS"},{"key":"10.1016\/j.vaccine.2010.05.079_bib6","unstructured":"Lee Che HR, Frasch CE. Polysaccharide\u2013protein conjugate vaccines. Patent pending, US 60\/493, 389; WO 2005\/014,037 A2; 2005."},{"key":"10.1016\/j.vaccine.2010.05.079_bib7","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1016\/j.vaccine.2006.12.033","article-title":"Investigation of the detoxification mechanism of formaldehyde-treated tetanus toxin","volume":"25","author":"Morten","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2010.05.079_bib8","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1128\/iai.52.2.519-528.1986","article-title":"Quantitative and qualitative analyses of serum antibodies elicited in adults by Haemophilus influenzae type b and pneumococcus type 6A capsular polysaccharide\u2013tetanus toxoid conjugates","volume":"52","author":"Schneerson","year":"1986","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2010.05.079_bib9","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1016\/S0264-410X(99)00370-9","article-title":"Activation of soluble polysaccharides with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) for use in protein\u2013polysaccharide conjugate vaccines and immunological reagents. II. Selective crosslinking of proteins to CDAP-activated polysaccharides","volume":"18","author":"Shafer","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2010.05.079_bib10","doi-asserted-by":"crossref","first-page":"4074","DOI":"10.1021\/bi00838a026","article-title":"The derivitization of cross-linked polyacrylamide beads. Controlled introduction of function groups for the preparation of special-purposes, biochemical absorbents","volume":"8","author":"Inman","year":"1969","journal-title":"Biochemistry"},{"key":"10.1016\/j.vaccine.2010.05.079_bib11","first-page":"23","article-title":"Acceptance criteria for levels of hydrazine in substance for pharmaceutical use and analytical methods for its determination","volume":"Notes 2","author":"Kean","year":"2006","journal-title":"Pharmeur Sci"},{"year":"2001","series-title":"Electrochemical methods: fundamentals and applications","author":"Bard","key":"10.1016\/j.vaccine.2010.05.079_bib12"},{"key":"10.1016\/j.vaccine.2010.05.079_bib13","unstructured":"INMETRO. Orienta\u00e7\u00e3o sobre Valida\u00e7\u00e3o de M\u00e9todos de Ensaios Qu\u00edmicos: DOQ-CGCRE-008. Revis\u00e3o 02. Rio de Janeiro; 2007. p. 24."},{"year":"1998","series-title":"The fitness for purpose of analytical methods, a laboratory guide to method validation and related topics","author":"EURACHEM","key":"10.1016\/j.vaccine.2010.05.079_bib14"},{"year":"1993","series-title":"Statistics for analytical chemistry","author":"Miller","key":"10.1016\/j.vaccine.2010.05.079_bib15"},{"key":"10.1016\/j.vaccine.2010.05.079_bib16","series-title":"International conference on harmonization of technical requirements for registration of pharmaceuticals for human use. Validation of analytical procedures: methodology","first-page":"8","author":"ICH","year":"1996"},{"year":"1997","series-title":"Toxicological profile for hydrazines","author":"ATSDR","key":"10.1016\/j.vaccine.2010.05.079_bib17"},{"key":"10.1016\/j.vaccine.2010.05.079_bib18","series-title":"International conference on harmonization of technical requirements for registration of pharmaceuticals for human use. Specifications: test procedures and acceptance criteria for biotechnological\/biological products. Q6B, current step 4 version","first-page":"9","author":"ICH","year":"1999"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X10008212?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X10008212?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:17:06Z","timestamp":1759400226000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X10008212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8]]},"references-count":18,"journal-issue":{"issue":"34","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["S0264410X10008212"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2010.05.079","relation":{},"ISSN":["0264-410X"],"issn-type":[{"type":"print","value":"0264-410X"}],"subject":[],"published":{"date-parts":[[2010,8]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Determination of hydrazine in a meningococcal C conjugate vaccine intermediary product","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2010.05.079","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}