{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T01:17:01Z","timestamp":1790299021492,"version":"4.1.0"},"reference-count":52,"publisher":"Elsevier BV","issue":"3","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2011,1]]},"DOI":"10.1016\/j.vaccine.2010.09.109","type":"journal-article","created":{"date-parts":[[2010,11,5]],"date-time":"2010-11-05T08:40:08Z","timestamp":1288946408000},"page":"577-582","source":"Crossref","is-referenced-by-count":17,"title":["The use of green fluorescent protein as a marker for Brucella vaccines"],"prefix":"10.1016","volume":"29","author":[{"given":"Carlos","family":"Chac\u00f3n-D\u00edaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Mu\u00f1oz-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"El\u00edas","family":"Barquero-Calvo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Guzm\u00e1n-Verri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Chaves-Olarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Jes\u00fas","family":"Grill\u00f3","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgardo","family":"Moreno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2010.09.109_bib0005","first-page":"315","article-title":"The genus Brucella","volume":"vol. 5","author":"Moreno","year":"2006"},{"key":"10.1016\/j.vaccine.2010.09.109_bib0010","first-page":"147","article-title":"Vaccination against Brucella","volume":"13","author":"Nicoletti","year":"1990","journal-title":"Adv Biotechnol Processes"},{"key":"10.1016\/j.vaccine.2010.09.109_bib0015","series-title":"Manual of diagnostic tests and vaccines for terrestrial animals, vol. 2","first-page":"1","article-title":"Bovine brucellosis","author":"Office International des \u00c9pizooties","year":"2009"},{"key":"10.1016\/j.vaccine.2010.09.109_bib0020","series-title":"Prevention of brucellosis in Mediterranean countries","first-page":"113","article-title":"Prevention of animal brucellosis: the role of the veterinary services","author":"Nicoletti","year":"1992"},{"issue":"12","key":"10.1016\/j.vaccine.2010.09.109_bib0025","doi-asserted-by":"crossref","first-page":"1575","DOI":"10.2460\/ajvr.1998.59.12.1575","article-title":"Protection against infection and abortion induced by virulent challenge exposure after oral vaccination of cattle with Brucella abortus strain RB51","volume":"59","author":"Elzer","year":"1998","journal-title":"Am J Vet Res"},{"issue":"1\u20134","key":"10.1016\/j.vaccine.2010.09.109_bib0030","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0378-1135(02)00255-9","article-title":"Brucellosis vaccines: past, present and future","volume":"90","author":"Schurig","year":"2002","journal-title":"Vet Microbiol"},{"issue":"12","key":"10.1016\/j.vaccine.2010.09.109_bib0035","doi-asserted-by":"crossref","first-page":"3136","DOI":"10.1128\/JCM.31.12.3136-3141.1993","article-title":"Comparative analysis of Brucella serotype A and M and Yersinia enterocolitica O:9 polysaccharides for serological diagnosis of brucellosis in cattle, sheep, and goats","volume":"31","author":"D\u00edaz-Aparicio","year":"1993","journal-title":"J Clin Microbiol"},{"issue":"1\u20134","key":"10.1016\/j.vaccine.2010.09.109_bib0040","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/0167-5877(84)90064-3","article-title":"Progres recents en immunisation contre l\u2019infection a immunisation chez les bovins","volume":"2","author":"Plommet","year":"1984","journal-title":"Prev Vet Med"},{"issue":"5","key":"10.1016\/j.vaccine.2010.09.109_bib0045","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1128\/CDLI.5.5.654-661.1998","article-title":"Enzyme immunoassays for serological diagnosis of bovine brucellosis: a trial in Latin America","volume":"5","author":"Gall","year":"1998","journal-title":"Clin Diagn Lab Immunol"},{"issue":"1","key":"10.1016\/j.vaccine.2010.09.109_bib0050","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1051\/vetres:2003037","article-title":"Rough vaccines in animal brucellosis: structural and genetic basis and present status","volume":"35","author":"Moriy\u00f3n","year":"2004","journal-title":"Vet Res"},{"issue":"7","key":"10.1016\/j.vaccine.2010.09.109_bib0055","doi-asserted-by":"crossref","first-page":"e2760","DOI":"10.1371\/journal.pone.0002760","article-title":"Brucellosis vaccines: assessment of Brucella melitensis lipopolysaccharide rough mutants defective in core and O-polysaccharide synthesis and export","volume":"3","author":"Gonz\u00e1lez","year":"2008","journal-title":"PLoS One"},{"issue":"3\u20134","key":"10.1016\/j.vaccine.2010.09.109_bib0060","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/S0167-5877(96)01110-5","article-title":"A review of the use of B. melitensis Rev 1 vaccine in adult sheep and goats","volume":"31","author":"Blasco","year":"1997","journal-title":"Prev Vet Med"},{"issue":"1","key":"10.1016\/j.vaccine.2010.09.109_bib0065","first-page":"131","article-title":"Vaccination against bovine brucellosis with a low dose of strain 19 administrated by the conjunctival route IV. Comparison between two methods of vaccination","volume":"10","author":"Fensterbank","year":"1979","journal-title":"Ann Res Vet"},{"issue":"5","key":"10.1016\/j.vaccine.2010.09.109_bib0070","doi-asserted-by":"crossref","first-page":"677","DOI":"10.2460\/ajvr.1996.57.05.677","article-title":"Protection of Balb\/c mice against homologous and heterologous species of Brucella by rough strain vaccines derived from Brucella melitensis and Brucella suis biovar 4","volume":"57","author":"Winter","year":"1996","journal-title":"Am J Vet Res"},{"issue":"6","key":"10.1016\/j.vaccine.2010.09.109_bib0075","doi-asserted-by":"crossref","first-page":"3261","DOI":"10.1128\/IAI.71.6.3261-3271.2003","article-title":"Characterization of Brucella abortus O-polysaccharide and core lipopolysaccharide mutants and demonstration that a complete core is required for rough vaccines to be efficient against Brucella abortus and Brucella ovis in the mouse model","volume":"71","author":"Monreal","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2010.09.109_bib0080","doi-asserted-by":"crossref","first-page":"1741","DOI":"10.1016\/j.vaccine.2009.01.025","article-title":"Rough mutants defective in core and O-polysaccharide synthesis and export induce antibodies reacting in an indirect ELISA with smooth lipopolysaccharide and are less effective than Rev 1 vaccine against Brucella melitensis infection of sheep","volume":"27","author":"Barrio","year":"2009","journal-title":"Vaccine"},{"issue":"5\u20136","key":"10.1016\/j.vaccine.2010.09.109_bib0085","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.cimid.2005.05.002","article-title":"Protection of Brucella abortus RB51 revaccinated cows","volume":"28","author":"Blasco","year":"2005","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"10.1016\/j.vaccine.2010.09.109_bib0090","doi-asserted-by":"crossref","DOI":"10.1016\/j.vaccine.2010.03.057","article-title":"Epidemiological study of brucellosis in cattle, immunized with Brucella abortus RB51 vaccine in endemic zones","author":"Herrera-L\u00f3pez","year":"2010","journal-title":"Vaccine"},{"issue":"1\u20134","key":"10.1016\/j.vaccine.2010.09.109_bib0095","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0378-1135(02)00242-0","article-title":"Brucellosis in Central America","volume":"90","author":"Moreno","year":"2002","journal-title":"Vet Microbiol"},{"issue":"1\u20132","key":"10.1016\/j.vaccine.2010.09.109_bib0100","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.prevetmed.2009.10.009","article-title":"Eradication of bovine brucellosis in the Azores Portugal: outcome of a 5-year programme (2002\u20132007) based on test-and-slaughter and RB51 vaccination","volume":"94","author":"Blasco","year":"2010","journal-title":"Prev Vet Med"},{"issue":"2","key":"10.1016\/j.vaccine.2010.09.109_bib0105","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1128\/IAI.65.2.798-800.1997","article-title":"Protection against infection in mice vaccinated with a Brucella abortus mutant","volume":"65","author":"Boschiroli","year":"1997","journal-title":"Infect Immun"},{"issue":"3\u20134","key":"10.1016\/j.vaccine.2010.09.109_bib0110","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.vetmic.2008.05.003","article-title":"Protection levels in vaccinated heifers with experimental vaccines Brucella abortus M1-luc and INTA 2","volume":"132","author":"Fiorentino","year":"2008","journal-title":"Vet Microbiol"},{"issue":"17","key":"10.1016\/j.vaccine.2010.09.109_bib0115","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.1016\/j.vaccine.2006.02.007","article-title":"Residual virulence and immunogenicity of CGV26 and CGV2631 B. melitensis Rev. 1 deletion mutant strains in sheep after subcutaneous or conjunctival vaccination","volume":"24","author":"Guilloteau","year":"2006","journal-title":"Vaccine"},{"issue":"2\u20133","key":"10.1016\/j.vaccine.2010.09.109_bib0120","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1574-6968.1996.tb08327.x","article-title":"Cloning, nucleotide sequence, and expression of the Brucella melitensis bp26 gene coding for a protein immunogenic in infected sheep","volume":"140","author":"Cloeckaert","year":"1996","journal-title":"FEMS Microbiol Lett"},{"issue":"3\u20134","key":"10.1016\/j.vaccine.2010.09.109_bib0125","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/S0378-1135(96)01265-5","article-title":"Competitive Enzyme-linked immunosorbent assay using monoclonal antibodies to the B. melitensis BP26 protein to evaluate antibody responses in infected and B. melitensis Rev.1 vaccinated sheep","volume":"53","author":"Debbarh","year":"1996","journal-title":"Vet Microbiol"},{"issue":"1","key":"10.1016\/j.vaccine.2010.09.109_bib0130","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/JCM.34.1.165-169.1996","article-title":"Cloning of Brucella abortus gene and characterization of expressed 26-kilodalton periplasmic protein: potential use for diagnosis","volume":"34","author":"Rossetti","year":"1996","journal-title":"J Clin Microbiol"},{"issue":"3","key":"10.1016\/j.vaccine.2010.09.109_bib0135","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0378-1135(02)00052-4","article-title":"Single-step purification and evaluation of recombinant BP26 protein for serological diagnosis of Brucella ovis infection in rams","volume":"87","author":"Zygmunt","year":"2002","journal-title":"Vet Microbiol"},{"issue":"15","key":"10.1016\/j.vaccine.2010.09.109_bib0140","doi-asserted-by":"crossref","first-page":"2910","DOI":"10.1016\/j.vaccine.2005.12.038","article-title":"Increases of efficacy as vaccine against Brucella abortus infection in mice by simultaneous inoculation with avirulent smooth bvrS\/bvrR and rough wbkA mutants","volume":"24","author":"Grill\u00f3","year":"2006","journal-title":"Vaccine"},{"issue":"5","key":"10.1016\/j.vaccine.2010.09.109_bib0145","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1016\/j.vaccine.2006.09.051","article-title":"Immunological responses and protective efficacy against Brucella melitensis induced by bp26 and omp31 B. melitensis Rev 1 deletion mutants in sheep","volume":"25","author":"Jacques","year":"2007","journal-title":"Vaccine"},{"issue":"3","key":"10.1016\/j.vaccine.2010.09.109_bib0150","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.biologicals.2005.04.003","article-title":"Evaluation of Brucella abortus S19 vaccine strains by bacteriological tests, molecular analysis of ery loci and virulence in BALB\/c mice","volume":"33","author":"Mukherjee","year":"2005","journal-title":"Biologicals"},{"issue":"4","key":"10.1016\/j.vaccine.2010.09.109_bib0155","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1084\/jem.20030088","article-title":"Brucella evades macrophage killing via VirB-dependent sustained interactions with the endoplasmic reticulum","volume":"198","author":"Celli","year":"2003","journal-title":"J Exp Med"},{"issue":"1","key":"10.1016\/j.vaccine.2010.09.109_bib0160","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/0378-1119(95)00584-1","article-title":"Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes","volume":"166","author":"Kovach","year":"1995","journal-title":"Gene"},{"issue":"48","key":"10.1016\/j.vaccine.2010.09.109_bib0165","doi-asserted-by":"crossref","first-page":"44435","DOI":"10.1074\/jbc.M105606200","article-title":"GTPases of the Rho subfamily are required for Brucella abortus internalization in nonprofessional phagocytes: direct activation of Cdc42","volume":"276","author":"Guzm\u00e1n-Verri","year":"2001","journal-title":"J Biol Chem"},{"issue":"5","key":"10.1016\/j.vaccine.2010.09.109_bib0170","doi-asserted-by":"crossref","first-page":"2387","DOI":"10.1128\/IAI.66.5.2387-2392.1998","article-title":"Virulent Brucella abortus prevents lysosome fusion and is distributed within autophagosome-like compartments","volume":"66","author":"Pizarro-Cerd\u00e1","year":"1998","journal-title":"Infect Immun"},{"issue":"2","key":"10.1016\/j.vaccine.2010.09.109_bib0175","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1006\/biol.2000.0249","article-title":"In vitro markers and biological activity in mice of seed lot strains and commercial Brucella melitensis Rev 1 and Brucella abortus B19 vaccines","volume":"28","author":"Grill\u00f3","year":"2000","journal-title":"Biologicals"},{"key":"10.1016\/j.vaccine.2010.09.109_bib0180","series-title":"Antibodies: a laboratory manual","author":"Harlow","year":"1988"},{"key":"10.1016\/j.vaccine.2010.09.109_bib0185","series-title":"Practical immunology","author":"Hudson","year":"1976"},{"key":"10.1016\/j.vaccine.2010.09.109_bib0190","article-title":"Methods in molecular biology","volume":"vol. 149","author":"Crowther","year":"2001"},{"issue":"8","key":"10.1016\/j.vaccine.2010.09.109_bib0195","doi-asserted-by":"crossref","first-page":"5137","DOI":"10.1128\/IAI.73.8.5137-5143.2005","article-title":"MyD88, but not toll-like receptors 4 and 2, is required for efficient clearance of Brucella abortus","volume":"73","author":"Weiss","year":"2005","journal-title":"Infect Immun"},{"issue":"2","key":"10.1016\/j.vaccine.2010.09.109_bib0200","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1006\/plas.1993.1016","article-title":"Conjugative transfer and in vitro\/in vivo stability of the broad-host-range IncP R751 plasmid in Brucella spp.","volume":"29","author":"Verger","year":"1993","journal-title":"Plasmid"},{"issue":"5","key":"10.1016\/j.vaccine.2010.09.109_bib0205","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1016\/0264-410X(90)90247-J","article-title":"Brucella suis S2, Brucella melitensis Rev. 1 and Brucella abortus S19 living vaccines: residual virulence and immunity induced against three Brucella species challenge strains in mice","volume":"8","author":"Bosseray","year":"1990","journal-title":"Vaccine"},{"issue":"SI","key":"10.1016\/j.vaccine.2010.09.109_bib0210","first-page":"103","article-title":"Construcci\u00f3n y caracterizaci\u00f3n de una mutante de Brucella abortus por inactivaci\u00f3n de un gen que codifica una prote\u00edna de 26kDa","volume":"27","author":"Boschiroli","year":"1995","journal-title":"Arch Med Vet"},{"issue":"21\u201322","key":"10.1016\/j.vaccine.2010.09.109_bib0215","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1016\/j.vaccine.2004.01.001","article-title":"Development and evaluation as vaccines in mice of Brucella melitensis Rev.1 single and double deletion mutants of the bp26 and omp31 genes coding for antigens of diagnostic significance in ovine brucellosis","volume":"22","author":"Cloeckaert","year":"2004","journal-title":"Vaccine"},{"issue":"2","key":"10.1016\/j.vaccine.2010.09.109_bib0220","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.vaccine.2008.10.065","article-title":"Efficacy of bp26 and bp26\/omp31 B. melitensis Rev.1 deletion mutants against Brucella ovis in rams","volume":"27","author":"Grill\u00f3","year":"2009","journal-title":"Vaccine"},{"issue":"13\u201314","key":"10.1016\/j.vaccine.2010.09.109_bib0225","doi-asserted-by":"crossref","first-page":"1820","DOI":"10.1016\/S0264-410X(02)00035-X","article-title":"Brucella abortus vaccine strain RB51 produces low levels of M-like O-antigen","volume":"20","author":"Cloeckaert","year":"2002","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.vaccine.2010.09.109_bib0230","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.cimid.2004.07.003","article-title":"Protection of Brucella abortus RB51 revaccinated cows, introduced in a herd with active brucellosis, with presence of atypical humoral response","volume":"28","author":"Leal-Hernandez","year":"2005","journal-title":"Comp Immunol Microbiol Infect Dis"},{"issue":"2","key":"10.1016\/j.vaccine.2010.09.109_bib0235","doi-asserted-by":"crossref","first-page":"143","DOI":"10.2460\/javma.1981.178.02.143","article-title":"Prevalence and persistence of Brucella abortus strain 19 infections and prevalence of other biotypes in vaccinated adult dairy cattle","volume":"178","author":"Nicoletti","year":"1981","journal-title":"J Am Vet Med Assoc"},{"issue":"19","key":"10.1016\/j.vaccine.2010.09.109_bib0240","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1136\/vr.165.19.570","article-title":"Abortions in pregnant dairy cows after vaccination with Brucella abortus strain RB51","volume":"165","author":"Yazdi","year":"2009","journal-title":"Vet Rec"},{"issue":"2","key":"10.1016\/j.vaccine.2010.09.109_bib0245","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/0378-1119(92)90691-H","article-title":"Primary structure of the Aequorea victoria green-fluorescent protein","volume":"111","author":"Prasher","year":"1992","journal-title":"Gene"},{"issue":"Pt 3","key":"10.1016\/j.vaccine.2010.09.109_bib0250","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1099\/0022-1317-81-3-709","article-title":"Development of a genetically marked recombinant rinderpest vaccine expressing green fluorescent protein","volume":"81","author":"Walsh","year":"2000","journal-title":"J Gen Virol"},{"issue":"21","key":"10.1016\/j.vaccine.2010.09.109_bib0255","doi-asserted-by":"crossref","first-page":"10165","DOI":"10.1128\/JVI.74.21.10165-10175.2000","article-title":"Recombinant rinderpest vaccines expressing membrane-anchored proteins as genetic markers: evidence of exclusion of marker protein from the virus envelope","volume":"74","author":"Walsh","year":"2000","journal-title":"J Virol"},{"issue":"5","key":"10.1016\/j.vaccine.2010.09.109_bib0260","doi-asserted-by":"crossref","first-page":"e2193","DOI":"10.1371\/journal.pone.0002193","article-title":"Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes","volume":"3","author":"Crasta","year":"2008","journal-title":"PLoS One"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X10015720?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X10015720?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T16:21:42Z","timestamp":1740673302000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X10015720"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":52,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["S0264410X10015720"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2010.09.109","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2011,1]]}}}