{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:09:54Z","timestamp":1791407394153,"version":"4.3.3"},"reference-count":31,"publisher":"Elsevier BV","issue":"22","license":[{"start":{"date-parts":[[2011,5,1]],"date-time":"2011-05-01T00:00:00Z","timestamp":1304208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2011,5]]},"DOI":"10.1016\/j.vaccine.2011.02.007","type":"journal-article","created":{"date-parts":[[2011,2,22]],"date-time":"2011-02-22T09:13:12Z","timestamp":1298365992000},"page":"3923-3929","source":"Crossref","is-referenced-by-count":35,"title":["Subcutaneous and intranasal immunization with Stx2B\u2013Tir\u2013Stx1B\u2013Zot reduces colonization and shedding of Escherichia coli O157:H7 in mice"],"prefix":"10.1016","volume":"29","author":[{"given":"Xue-han","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kong-wang","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-xia","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-cai","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pan-deng","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-ting","family":"Luan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-bin","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong-li","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-min","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-xin","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-ming","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng-yu","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai-hua","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2011.02.007_bib0005","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1046\/j.1365-2958.1998.00798.x","article-title":"EspE, a novel secreted protein of attaching and effacing bacteria, is directly translocated into infected host cells, where it appears as a tyrosine-phosphorylated 90kDa protein","volume":"28","author":"Deibel","year":"1998","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0010","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1007\/PL00013202","article-title":"Enteropathogenic Escherichia coli: a pathogen that inserts its own receptor into host cells","volume":"55","author":"DeVinney","year":"1999","journal-title":"Cell Mol Life Sci"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0015","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/S0092-8674(00)80437-7","article-title":"Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells","volume":"91","author":"Kenny","year":"1997","journal-title":"Cell"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0020","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/S1369-5274(03)00005-5","article-title":"Tails of two Tirs: actin pedestal formation by enteropathogenic E. coli and enterohemorrhagic E. coli O157:H7","volume":"6","author":"Campellone","year":"2000","journal-title":"Curr Opin Microbiol"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0025","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.devcel.2004.07.004","article-title":"EspFU is a translocated EHEC effector that interacts with Tir and N-WASP and promotes Nck-independent actin assembly","volume":"7","author":"Campellone","year":"2004","journal-title":"Dev Cell"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0030","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.1111\/j.1462-5822.2004.00459.x","article-title":"TccP is an enterohaemorrhagic Escherichia coli O157:H7 type III effector protein that couples Tir to the actin-cytoskeleton","volume":"6","author":"Garmendia","year":"2004","journal-title":"Cell Microbiol"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0035","doi-asserted-by":"crossref","first-page":"2541","DOI":"10.1128\/IAI.73.4.2541-2546.2005","article-title":"Antibody against the carboxyl terminus of intimin alpha reduces enteropathogenic Escherichia coli adherence to tissue culture cells and subsequent induction of actin polymerization","volume":"73","author":"Carvalho","year":"2005","journal-title":"Infect Immun"},{"issue":"12","key":"10.1016\/j.vaccine.2011.02.007_bib0040","first-page":"2570","article-title":"Cloning, expression, biological activities of recombinant Tir gene from enterohemorrhagic Escherichia coli O157:H7","volume":"43","author":"Zhang","year":"2010","journal-title":"Sci Agric Sin"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0045","doi-asserted-by":"crossref","first-page":"7129","DOI":"10.1128\/IAI.71.12.7129-7139.2003","article-title":"Critical roles for stx2, eae, and tir in enterohemorrhagic Escherichia coli-induced diarrhea and intestinal inflammation in infant rabbits","volume":"71","author":"Ritchie","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0050","doi-asserted-by":"crossref","first-page":"823","DOI":"10.1007\/s004670100660","article-title":"Pathogenesis of Shiga toxin-induced hemolytic uremic syndrome","volume":"16","author":"Ray","year":"2001","journal-title":"Pediatr Nephrol"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0055","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1128\/AEM.01328-06","article-title":"Shiga toxin and Shiga toxin-encoding phage do not facilitate Escherichia coli O157:H7 colonization in sheep","volume":"73","author":"Cornick","year":"2007","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0060","doi-asserted-by":"crossref","first-page":"9667","DOI":"10.1073\/pnas.0602359103","article-title":"Shiga toxin of enterohemorrhagic Escherichia coli type O157:H7 promotes intestinal colonization","volume":"103","author":"Robinson","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0065","doi-asserted-by":"crossref","first-page":"2209","DOI":"10.1128\/IAI.67.5.2209-2217.1999","article-title":"Shiga toxin 1 from Escherichia coli blocks activation and proliferation of bovine lymphocyte subpopulations in vitro","volume":"67","author":"Menge","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0070","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.micinf.2006.10.005","article-title":"Shiga toxin binding in normal and inflamed human intestinal mucosa","volume":"9","author":"Schuller","year":"2007","journal-title":"Microb Infect"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0075","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1078\/1438-4221-00202","article-title":"Consequences of EHEC colonisation in humans and cattle","volume":"292","author":"Smith","year":"2002","journal-title":"Int J Med Microbiol"},{"issue":"15","key":"10.1016\/j.vaccine.2011.02.007_bib0080","doi-asserted-by":"crossref","first-page":"4975","DOI":"10.1128\/AEM.00297-09","article-title":"Adherence of Escherichia coli O157:H7 mutants in vitro and in ligated pig intestines","volume":"75","author":"Yin","year":"2009","journal-title":"Appl Environ Microb"},{"issue":"13","key":"10.1016\/j.vaccine.2011.02.007_bib0085","doi-asserted-by":"crossref","first-page":"4461","DOI":"10.1128\/AEM.00182-10","article-title":"Verotoxin 2 enhances adherence of enterohemorrhagic Escherichia coli O157:H7 to intestinal epithelial cells and expression of \u03b21-integrin by IPEC-J2 Cells","volume":"76","author":"Liu","year":"2010","journal-title":"Appl Environ Microb"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0090","doi-asserted-by":"crossref","first-page":"2070","DOI":"10.1016\/j.vaccine.2009.01.115","article-title":"Immunogenicity of a novel Stx2B\u2013Stx1B fusion protein in a mice model of enterohemorrhagic Escherichia coli O157:H7 infection","volume":"27","author":"Gao","year":"2009","journal-title":"Vaccine"},{"issue":"22","key":"10.1016\/j.vaccine.2011.02.007_bib0095","doi-asserted-by":"crossref","first-page":"19160","DOI":"10.1074\/jbc.M009674200","article-title":"Zonula Occludens toxin structure\u2013function analysis","volume":"276","author":"Di Pierro","year":"2001","journal-title":"J Biol Chem"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0100","first-page":"S260","article-title":"Zonula occludens toxin as a new promising adjuvant for mucosal vaccines","volume":"24S2","author":"De Magistris","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0105","doi-asserted-by":"crossref","first-page":"710","DOI":"10.1172\/JCI118114","article-title":"Zonula occludens toxin modulates tight junctions through protein kinase C-dependent actin reorganization, in vitro","volume":"96","author":"Fasano","year":"1995","journal-title":"J Clin Investig"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0110","doi-asserted-by":"crossref","first-page":"1158","DOI":"10.1172\/JCI119271","article-title":"Modulation of intestinal tight junctions by Zonula occludens toxin permits enteral administration of insulin and other macromolecules in an animal model","volume":"99","author":"Fasano","year":"1997","journal-title":"J Clin Investig"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2001.tb09437.x","article-title":"Purification and preliminary characterization of the zonula occludens toxin receptor from human (CaCo-2) and murine (IEC6) intestinal cell lines","volume":"194","author":"Uzzau","year":"2001","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0120","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/j.1749-6632.2000.tb05244.x","article-title":"Regulation of intercellular tight junctions by Zonula occludens toxin and its eukaryotic analogue zonulin","volume":"915","author":"Fasano","year":"2000","journal-title":"Ann NY Acad Sci"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0125","doi-asserted-by":"crossref","first-page":"4435","DOI":"10.1242\/jcs.113.24.4435","article-title":"Human zonulin, a potential modulator of intestinal tight junctions","volume":"24","author":"Wang","year":"2000","journal-title":"J Cell Sci"},{"issue":"4","key":"10.1016\/j.vaccine.2011.02.007_bib0130","doi-asserted-by":"crossref","first-page":"1897","DOI":"10.1128\/IAI.71.4.1897-1902.2003","article-title":"Zonula Occludens toxin acts as an adjuvant through different mucosal routes and induces protective immune responses","volume":"71","author":"Marinaro","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0135","doi-asserted-by":"crossref","first-page":"2438","DOI":"10.1128\/IAI.58.8.2438-2445.1990","article-title":"Mouse model for colonization and disease caused by enterohemorrhagic Escherichia coli O157:H7","volume":"58","author":"Wadolkowski","year":"1990","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0140","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.micpath.2008.01.005","article-title":"Subcutaneous and intranasal immunization with type III secreted proteins can prevent colonization and shedding of Escherichia coli O157:H7 in mice","volume":"45","author":"Babiuk","year":"2008","journal-title":"Microb Pathog"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0145","doi-asserted-by":"crossref","first-page":"5242","DOI":"10.1073\/pnas.88.12.5242","article-title":"Vibrio cholerae produces a second enterotoxin, which affects intestinal tight junctions","volume":"88","author":"Fasano","year":"1991","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0150","doi-asserted-by":"crossref","first-page":"4778","DOI":"10.4049\/jimmunol.165.9.4778","article-title":"The mucosal adjuvant cholera toxin redirects vaccine proteins into olfactory tissues","volume":"165","author":"van Ginkel","year":"2000","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2011.02.007_bib0155","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1128\/IAI.67.3.1287-1291.1999","article-title":"Zonula occludens toxin is a powerful mucosal adjuvant for intranasally delivered antigens","volume":"67","author":"Marinaro","year":"1999","journal-title":"Infect Immun"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X11002088?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X11002088?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2021,11,19]],"date-time":"2021-11-19T08:38:56Z","timestamp":1637311136000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X11002088"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5]]},"references-count":31,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["S0264410X11002088"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2011.02.007","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2011,5]]}}}