{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:57:57Z","timestamp":1791035877849,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","issue":"12","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"ERDF","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005357","name":"Slovak Research and Development Agency","doi-asserted-by":"crossref","award":["APVV-51-004505"],"award-info":[{"award-number":["APVV-51-004505"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Slovak Scientific Grant Agency VEGA","award":["2\/0191\/12"],"award-info":[{"award-number":["2\/0191\/12"]}]},{"name":"MEC"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2013,3]]},"DOI":"10.1016\/j.vaccine.2013.01.017","type":"journal-article","created":{"date-parts":[[2013,1,25]],"date-time":"2013-01-25T18:30:44Z","timestamp":1359138644000},"page":"1582-1589","source":"Crossref","is-referenced-by-count":15,"title":["Immunization with recombinant subolesin does not reduce tick infection with tick-borne encephalitis virus nor protect mice against disease"],"prefix":"10.1016","volume":"31","author":[{"given":"Sab\u00edna","family":"Havl\u00edkov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Li\u010dkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieves","family":"Ayll\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ladislav","family":"Roller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1ria","family":"Kazim\u00edrov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirko","family":"Slov\u00e1k","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Moreno-Cid","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 M.","family":"P\u00e9rez de la Lastra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Klempa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9","family":"de la Fuente","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2013.01.017_bib0005","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S0065-3527(03)61008-0","article-title":"Tick-borne flaviviruses","volume":"61","author":"Gritsun","year":"2003","journal-title":"Adv Virus Res"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0010","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0166-3542(02)00206-1","article-title":"Tick-borne encephalitis","volume":"57","author":"Gritsun","year":"2003","journal-title":"Antiviral Res"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0015","doi-asserted-by":"crossref","first-page":"19976","DOI":"10.2807\/ese.16.39.19976-en","article-title":"Working group for tick-borne encephalitis virus C. Tick-borne encephalitis in Europe, 2007 to 2009","volume":"16","author":"Donoso Mantke","year":"2011","journal-title":"Euro Surveill"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0020","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.ttbdis.2010.10.007","article-title":"Tick-borne encephalitis 2010: epidemiology, risk areas, and virus strains in Europe and Asia\u2014an overview","volume":"2","author":"S\u00fcss","year":"2011","journal-title":"Ticks Tick Borne Dis"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0025","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1186\/1756-3305-5-184","article-title":"Why is tick-borne encephalitis increasing? A review of the key factors causing the increasing incidence of human TBE in Sweden","volume":"5","author":"Jaenson","year":"2012","journal-title":"Parasit Vectors"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0030","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1093\/jmedent\/30.1.295","article-title":"Efficient transmission of tick-borne encephalitis virus between cofeeding ticks","volume":"30","author":"Labuda","year":"1993","journal-title":"J Med Entomol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0035","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1006\/viro.1996.0261","article-title":"Importance of localized skin infection in tick-borne encephalitis virus transmission","volume":"219","author":"Labuda","year":"1996","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0040","doi-asserted-by":"crossref","first-page":"4301","DOI":"10.1016\/j.vaccine.2011.09.114","article-title":"Flaviviruses and flavivirus vaccines","volume":"30","author":"Heinz","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0045","doi-asserted-by":"crossref","first-page":"2827","DOI":"10.1016\/j.vaccine.2010.02.001","article-title":"Vaccine failures after active immunisation against tick-borne encephalitis","volume":"28","author":"Andersson","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0050","doi-asserted-by":"crossref","first-page":"7307","DOI":"10.1016\/j.vaccine.2011.07.089","article-title":"Prevention of tick-borne encephalitis by FSME-IMMUN vaccines: review of a clinical development programme","volume":"29","author":"Loew-Baselli","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0055","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1586\/14760584.7.5.589","article-title":"Advances in vaccination against tick-borne encephalitis","volume":"7","author":"Rendi-Wagner","year":"2008","journal-title":"Expert Rev Vaccines"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0060","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1586\/14760584.2.4.583","article-title":"Advances in the identification and characterization of protective antigens for development of recombinant vaccines against tick infestations","volume":"2","author":"de la Fuente","year":"2003","journal-title":"Expert Rev Vaccines"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0065","first-page":"880","article-title":"Advances in flavivirus vaccine development","volume":"13","author":"Coller","year":"2010","journal-title":"IDrugs"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0070","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1590\/S0074-02762007005000054","article-title":"Perspectives in the control of infectious diseases by transgenic mosquitoes in the post-genomic era\u2014a review","volume":"102","author":"Speran\u00e7a","year":"2007","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0075","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/S0264-410X(97)00208-9","article-title":"Field studies and cost-effectiveness analysis of vaccination with Gavac\u2122 against the cattle tick Boophilus microplus","volume":"16","author":"de la Fuente","year":"1998","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0080","doi-asserted-by":"crossref","first-page":"1608","DOI":"10.1111\/j.1469-0691.2011.03664.x","article-title":"Vector control: a cornerstone in the malaria elimination campaign","volume":"17","author":"Karunamoorthi","year":"2011","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0085","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1017\/S1466252307001193","article-title":"A ten-year review of commercial vaccine performance for control of tick infestations on cattle","volume":"8","author":"de la Fuente","year":"2007","journal-title":"Anim Health Res Rev"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0090","doi-asserted-by":"crossref","first-page":"8575","DOI":"10.1016\/j.vaccine.2011.09.023","article-title":"Targeting the tick protective antigen subolesin reduces vector infestations and pathogen infection by Anaplasma marginale and Babesia bigemina","volume":"29","author":"Merino","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0095","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.vetpar.2011.04.018","article-title":"Targeting arthropod subolesin\/akirin for the development of a universal vaccine for control of vector infestations and pathogen transmission","volume":"181","author":"de la Fuente","year":"2011","journal-title":"Vet Parasitol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0100","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/s00436-009-1368-2","article-title":"Conservation and immunogenicity of the mosquito ortholog of the tick protective antigen, subolesin","volume":"105","author":"Canales","year":"2009","journal-title":"Parasitol Res"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0105","doi-asserted-by":"crossref","first-page":"4082","DOI":"10.1016\/j.vaccine.2006.02.046","article-title":"The tick protective antigen, 4D8, is a conserved protein involved in modulation of tick blood ingestion and reproduction","volume":"24","author":"de la Fuente","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0110","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1186\/1471-2148-9-34","article-title":"Evolution of the multifaceted eukaryotic akirin gene family","volume":"9","author":"Macqueen","year":"2009","journal-title":"BMC Evol Biol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0115","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.dci.2008.11.002","article-title":"Tick subolesin is an ortholog of the akirins described in insects and vertebrates","volume":"33","author":"Galindo","year":"2009","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0120","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1016\/j.dci.2009.02.010","article-title":"Response to the commentary of D. Macqueen on: Galindo RC, Doncel-P\u00e9rez E, Zivkovic Z, Naranjo V, Gortazar C, Mangold AJ, et al. Tick subolesin is an ortholog of the akirins described in insects and vertebrates [Dev. Comp. Immunol. 33 (2009) 612\u2013617]","volume":"33","author":"Mangold","year":"2009","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0125","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1038\/ni1543","article-title":"Akirins are highly conserved nuclear proteins required for NF-kappaB-dependent gene expression in drosophila and mice","volume":"9","author":"Goto","year":"2008","journal-title":"Nat Immunol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0130","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1186\/1471-2164-9-372","article-title":"Evidence of the role of tick subolesin in gene expression","volume":"9","author":"de la Fuente","year":"2008","journal-title":"BMC Genomics"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0135","doi-asserted-by":"crossref","first-page":"e1002547","DOI":"10.1371\/journal.pgen.1002547","article-title":"Akirin links twist-regulated transcription with the Brahma chromatin remodeling complex during embryogenesis","volume":"8","author":"Nowak","year":"2012","journal-title":"PLoS Genet"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0140","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.bbrc.2006.03.109","article-title":"Autocidal control of ticks by silencing of a single gene by RNA interference","volume":"344","author":"de la Fuente","year":"2006","journal-title":"Biochem Biophys Res Commun"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0145","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1186\/1471-213X-9-42","article-title":"Silencing of genes involved in Anaplasma marginale-tick interactions affects the pathogen developmental cycle in Dermacentor variabilis","volume":"9","author":"Kocan","year":"2009","journal-title":"BMC Dev Biol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0150","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/1471-2172-11-7","article-title":"Subolesin expression in response to pathogen infection in ticks","volume":"11","author":"Zivkovic","year":"2010","journal-title":"BMC Immunol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0155","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1016\/S0264-410X(02)00683-7","article-title":"Identification of protective antigens for the control of Ixodes scapularis infestations using cDNA expression library immunization","volume":"21","author":"Almaz\u00e1n","year":"2003","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0160","doi-asserted-by":"crossref","first-page":"6040","DOI":"10.1016\/j.vaccine.2012.07.053","article-title":"Oral vaccination with vaccinia virus expressing the tick antigen subolesin inhibits tick feeding and transmission of Borrelia burgdorferi","volume":"30","author":"Bensaci","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0165","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1006\/abio.1995.1126","article-title":"Substitution of chloroform by bromo-chloropropane in the single-step method of RNA isolation","volume":"225","author":"Chomczynski","year":"1995","journal-title":"Anal Biochem"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0170","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1111\/j.1365-2915.2011.00973.x","article-title":"Expression of heat-shock proteins and subolesin affects stress responses, Anaplasma phagocytophilum infection and questing behavior in the tick, Ixodes scapularis","volume":"26","author":"Busby","year":"2012","journal-title":"Med Vet Entomol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0175","doi-asserted-by":"crossref","first-page":"14211","DOI":"10.1073\/pnas.0603459103","article-title":"Central peptidergic ensembles associated with organisation of an innate behavior","volume":"103","author":"Kim","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0180","doi-asserted-by":"crossref","first-page":"1147","DOI":"10.1016\/j.ibmb.2008.04.009","article-title":"The unique evolution of neuropeptide genes in the silkworm Bombyx mori","volume":"38","author":"Roller","year":"2008","journal-title":"Insect Biochem Mol Biol"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0185","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1007\/s00436-009-1689-1","article-title":"Identification and characterization of Rhipicephalus (Boophilus) microplus candidate protective antigens for the control of cattle tick infestations","volume":"106","author":"Almaz\u00e1n","year":"2010","journal-title":"Parasitol Res"},{"issue":"4","key":"10.1016\/j.vaccine.2013.01.017_bib0190","doi-asserted-by":"crossref","first-page":"e27","DOI":"10.1371\/journal.ppat.0020027","article-title":"An antivector vaccine protects against a lethal vector-borne pathogen","volume":"2","author":"Labuda","year":"2006","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0195","first-page":"113","article-title":"A simple micro-culture method for the study of group B arboviruses","volume":"40","author":"De Madrid","year":"1969","journal-title":"Bull World Health Organ"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0200","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1196\/annals.1428.056","article-title":"Differential expression of the tick protective antigen subolesin in Anaplasma marginale- and A. phagocytophilum-infected host cells","volume":"1149","author":"de la Fuente","year":"2008","journal-title":"Ann NY Acad Sci"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0205","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ttbdis.2011.09.003","article-title":"Differential salivary gland transcript expression profile in Ixodes scapularis nymphs upon feeding or flavivirus infection","volume":"3","author":"McNally","year":"2012","journal-title":"Ticks Tick Borne Dis"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0210","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1007\/s00436-006-0244-6","article-title":"Reduction of tick infections with Anaplasma marginale and A. phagocytophilum by targeting the tick protective antigen subolesin","volume":"100","author":"de la Fuente","year":"2006","journal-title":"Parasitol Res"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0215","doi-asserted-by":"crossref","first-page":"4403","DOI":"10.1016\/j.vaccine.2005.04.012","article-title":"Characterization of three Ixodes scapularis cDNAs protective against tick infestations","volume":"23","author":"Almaz\u00e1n","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0220","doi-asserted-by":"crossref","first-page":"2248","DOI":"10.1016\/j.vaccine.2011.01.050","article-title":"Control of Rhipicephalus (Boophilus) microplus infestations by the combination of subolesin vaccination and tick autocidal control after subolesin gene knockdown in ticks fed on cattle","volume":"29","author":"Merino","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.01.017_bib0225","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.vaccine.2011.10.099","article-title":"Vaccination with BM86, subolesin and akirin protective antigens for the control of tick infestations in white tailed deer and red deer","volume":"30","author":"Carre\u00f3n","year":"2012","journal-title":"Vaccine"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X13000741?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X13000741?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,7]],"date-time":"2020-05-07T07:38:52Z","timestamp":1588837132000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X13000741"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":45,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["S0264410X13000741"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2013.01.017","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2013,3]]}}}