{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T14:25:59Z","timestamp":1779114359707,"version":"3.51.4"},"reference-count":25,"publisher":"Elsevier BV","issue":"11","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2013,3]]},"DOI":"10.1016\/j.vaccine.2013.01.030","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T09:02:33Z","timestamp":1359536553000},"page":"1458-1463","source":"Crossref","is-referenced-by-count":25,"title":["Oral immunization of a live attenuated Escherichia coli strain expressing a holotoxin-structured adhesin\u2013toxoid fusion (1FaeG-FedF-LTA2:5LTB) protected young pigs against enterotoxigenic E. coli (ETEC) infection"],"prefix":"10.1016","volume":"31","author":[{"given":"Xiaosai","family":"Ruan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2013.01.030_bib0005","series-title":"The World Health Report 1999: making a difference","author":"WHO","year":"1999"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0010","series-title":"USDA:APHIS:VS, CEAH. Nat. Anim Health Monitoring Syst., in Part II: reference for swine health and health management in the United States","author":"USDA","year":"2000"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0015","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1079\/AHRR200131","article-title":"Identification of virulence attributes of gastrointestinal Escherichia coli isolates of veterinary significance","volume":"2","author":"DebRoy","year":"2001","journal-title":"Anim Health Res Rev"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0020","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1128\/CMR.11.1.142","article-title":"Diarrheagenic Escherichia coli","volume":"11","author":"Nataro","year":"1998","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0025","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0378-1135(01)00504-1","article-title":"Prevalence of serogroups and virulence genes in Escherichia coli associated with postweaning diarrhoea and edema disease in pigs and a comparison of diagnostic approaches","volume":"85","author":"Frydendahl","year":"2002","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0030","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.vetmic.2007.02.018","article-title":"Prevalence of virulence genes in Escherichia coli strains recently isolated from young pigs with diarrhea in the US","volume":"123","author":"Zhang","year":"2007","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0035","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1007\/s11250-011-9786-4","article-title":"High prevalence of F4+ and F18+ Escherichia coli in Cuban piggeries as determined by serological survey","volume":"43","author":"de la Fe Rodriguez","year":"2011","journal-title":"Trop Anim Health Product"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0040","first-page":"259","article-title":"Enterotoxigenic Escherichia coli (ETEC) in farm animals","volume":"30","author":"Nagy","year":"1999","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0045","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1079\/AHR2005105","article-title":"Escherichia coli in postweaning diarrhea in pigs: an update on bacterial types, pathogenesis, and prevention strategies","volume":"6","author":"Fairbrother","year":"2005","journal-title":"Anim Health Res Rev"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0050","doi-asserted-by":"crossref","first-page":"1593","DOI":"10.1128\/CVI.05120-11","article-title":"A tripartite fusion, FaeG-FedF-LT(192)A2:B, of enterotoxigenic Escherichia coli (ETEC) elicits antibodies that neutralize cholera toxin, inhibit adherence of K88 (F4) and F18 fimbriae, and protect pigs against K88ac\/heat-labile toxin infection","volume":"18","author":"Ruan","year":"2011","journal-title":"Clin Vaccine Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0055","doi-asserted-by":"crossref","first-page":"4915","DOI":"10.1128\/IAI.60.11.4915-4924.1992","article-title":"Fusion proteins containing the A2 domain of cholera toxin assemble with B polypeptides of cholera toxin to form immunoreactive and functional holotoxin-like chimeras","volume":"60","author":"Jobling","year":"1992","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0060","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1128\/IAI.66.2.462-468.1998","article-title":"Mucosal immunogenicity of a holotoxin-like molecule containing the serine-rich Entamoeba histolytica protein (SREHP) fused to the A2 domain of cholera toxin","volume":"66","author":"Sultan","year":"1998","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0065","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.2460\/ajvr.1991.52.07.1051","article-title":"Evaluation of a live avirulent Escherichia coli vaccine for K88+, LT+ enterotoxigenic colibacillosis in weaned pigs","volume":"52","author":"Francis","year":"1991","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0070","doi-asserted-by":"crossref","first-page":"5755","DOI":"10.1128\/IAI.67.11.5755-5761.1999","article-title":"Lysine residue 117 of the FasG adhesin of enterotoxigenic Escherichia coli is essential for binding of 987P fimbriae to sulfatide","volume":"67","author":"Choi","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0075","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1128\/IAI.00497-09","article-title":"Genetic fusions of heat-labile (LT) and heat-stable (ST) toxoids of porcine enterotoxigenic Escherichia coli elicit neutralizing anti-LT and anti-STa antibodies","volume":"78","author":"Zhang","year":"2010","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0080","unstructured":"Ausubel FM, Brent R, Kingston RK, Moore DD, Seidman JG, Smith JA, et al. Short protocols in molecular biology. 4th ed. New York, NY: John Wiley & Sons, Inc.; 1999."},{"key":"10.1016\/j.vaccine.2013.01.030_bib0085","series-title":"Guide for the care and use of laboratory animals","year":"1996"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0090","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/j.vetmic.2007.12.018","article-title":"Porcine intestinal epithelial cell lines as a new in vitro model for studying adherence and pathogenesis of enterotoxigenic Escherichia coli","volume":"130","author":"Koh","year":"2008","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0095","doi-asserted-by":"crossref","first-page":"3107","DOI":"10.1128\/IAI.01338-05","article-title":"Significance of heat-stable and heat-labile enterotoxins in porcine colibacillosis in an additive model for pathogenicity studies","volume":"74","author":"Zhang","year":"2006","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0100","doi-asserted-by":"crossref","first-page":"3914","DOI":"10.1128\/IAI.72.7.3914-3924.2004","article-title":"Relative importance of heat-labile enterotoxin in the causation of severe diarrheal disease in the gnotobiotic piglet model by a strain of enterotoxigenic Escherichia coli that produces multiple enterotoxins","volume":"72","author":"Berberov","year":"2004","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0105","doi-asserted-by":"crossref","first-page":"3141","DOI":"10.1128\/IAI.01743-07","article-title":"Comparison of the contributions of heat-labile enterotoxin and heat-stable enterotoxin b to the virulence of enterotoxigenic Escherichia coli in F4ac receptor-positive young pigs","volume":"76","author":"Erume","year":"2008","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0110","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1128\/CVI.00095-10","article-title":"Genetic fusions of heat-labile toxoid (LT) and heat-stable toxin b (STb) of porcine enterotoxigenic Escherichia coli elicit protective anti-LT and anti-STb antibodies","volume":"17","author":"Zhang","year":"2010","journal-title":"Clin Vaccine Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0115","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1128\/IAI.58.5.1159-1166.1990","article-title":"Construction of a nontoxic fusion peptide for immunization against Escherichia coli strains that produce heat-labile and heat-stable enterotoxins","volume":"58","author":"Clements","year":"1990","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0120","doi-asserted-by":"crossref","first-page":"4789","DOI":"10.1128\/IAI.62.11.4789-4794.1994","article-title":"Age-related resistance to 987P fimbria-mediated colonization correlates with specific glycolipid receptors in intestinal mucus in swine","volume":"62","author":"Dean-Nystrom","year":"1994","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.01.030_bib0125","doi-asserted-by":"crossref","first-page":"890","DOI":"10.1006\/jmbi.1993.1209","article-title":"Refined structure of Escherichia coli heat-labile enterotoxin, a close relative of cholera tocin","volume":"230","author":"Sixma","year":"1993","journal-title":"J Mol Biol"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X1300087X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X1300087X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,5,5]],"date-time":"2024-05-05T08:41:35Z","timestamp":1714898495000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X1300087X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3]]},"references-count":25,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["S0264410X1300087X"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2013.01.030","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2013,3]]}}}