{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T06:06:34Z","timestamp":1792649194692,"version":"4.5.0"},"reference-count":35,"publisher":"Elsevier BV","issue":"24","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill & Melinda Gates Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2013,5]]},"DOI":"10.1016\/j.vaccine.2013.04.012","type":"journal-article","created":{"date-parts":[[2013,4,17]],"date-time":"2013-04-17T15:08:27Z","timestamp":1366211307000},"page":"2667-2672","source":"Crossref","is-referenced-by-count":41,"title":["Parenteral immunization with IpaB\/IpaD protects mice against lethal pulmonary infection by Shigella"],"prefix":"10.1016","volume":"31","author":[{"given":"Francisco J.","family":"Martinez-Becerra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Micah","family":"Scobey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Harrison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shyamal P.","family":"Choudhari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy M.","family":"Quick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangeeta B.","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Russell","family":"Middaugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy L.","family":"Picking","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"July, 7","key":"10.1016\/j.vaccine.2013.04.012_bib0005","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1038\/nrmicro1662","article-title":"Clinical trials of Shigella vaccines: two steps forward and one step back on a long, hard road","volume":"5","author":"Levine","year":"2007","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.vaccine.2013.04.012_bib0010","unstructured":"WHO. Shigellosis. 2009 [cited; Available from: http:\/\/www.who.int\/vaccine_research\/diseases\/diarrhoeal\/en\/index6.html."},{"issue":"November, 5","key":"10.1016\/j.vaccine.2013.04.012_bib0015","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.jinf.2011.08.003","article-title":"Epidemiology of food-borne disease outbreaks in the French armed forces: a review of investigations conducted from 1999 to 2009","volume":"63","author":"Mayet","year":"2011","journal-title":"J Infect"},{"issue":"2","key":"10.1016\/j.vaccine.2013.04.012_bib0020","doi-asserted-by":"crossref","first-page":"e4494","DOI":"10.1371\/journal.pone.0004494","article-title":"A look back at an ongoing problem: Shigella dysenteriae type 1 epidemics in refugee settings in Central Africa (1993\u20131995)","volume":"4","author":"Kerneis","year":"2009","journal-title":"PloS one"},{"issue":"7","key":"10.1016\/j.vaccine.2013.04.012_bib0025","doi-asserted-by":"crossref","first-page":"2548","DOI":"10.1128\/IAI.06391-11","article-title":"Hierarchies of host factor dynamics at the entry site of Shigella flexneri during host cell invasion","volume":"80","author":"Ehsani","year":"2012","journal-title":"Infect Immun"},{"issue":"July","key":"10.1016\/j.vaccine.2013.04.012_bib0030","article-title":"Emergence of a novel Shigella flexneri serotype 1d in China","volume":"31","author":"Luo","year":"2012","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"January, 1","key":"10.1016\/j.vaccine.2013.04.012_bib0035","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0966-842X(99)01646-7","article-title":"Serotype-converting bacteriophages and O-antigen modification in Shigella flexneri","volume":"8","author":"Allison","year":"2000","journal-title":"Trends Microbiol"},{"issue":"December, 49","key":"10.1016\/j.vaccine.2013.04.012_bib0040","doi-asserted-by":"crossref","first-page":"32895","DOI":"10.1074\/jbc.273.49.32895","article-title":"Shigella-induced apoptosis is dependent on caspase-1 which binds to IpaB","volume":"273","author":"Hilbi","year":"1998","journal-title":"J Biol Chem"},{"issue":"February, 4","key":"10.1016\/j.vaccine.2013.04.012_bib0045","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1111\/j.1365-2958.1994.tb00341.x","article-title":"IpaB mediates macrophage apoptosis induced by Shigella flexneri","volume":"11","author":"Zychlinsky","year":"1994","journal-title":"Mol Microbiol"},{"issue":"April, 4","key":"10.1016\/j.vaccine.2013.04.012_bib0050","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.chom.2012.03.001","article-title":"Shigella targets epithelial tricellular junctions and uses a noncanonical clathrin-dependent endocytic pathway to spread between cells","volume":"11","author":"Fukumatsu","year":"2012","journal-title":"Cell Host Microbe"},{"issue":"November, 6","key":"10.1016\/j.vaccine.2013.04.012_bib0055","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1111\/j.1574-6976.2011.00271.x","article-title":"Functional domains and motifs of bacterial type III effector proteins and their roles in infection","volume":"35","author":"Dean","year":"2011","journal-title":"FEMS Microbiol Rev"},{"issue":"December, 12","key":"10.1016\/j.vaccine.2013.04.012_bib0060","doi-asserted-by":"crossref","first-page":"5281","DOI":"10.1128\/IAI.00800-09","article-title":"Spa15 of Shigella flexneri is secreted through the type III secretion system and prevents staurosporine-induced apoptosis","volume":"77","author":"Faherty","year":"2009","journal-title":"Infect Immun"},{"issue":"August, 4","key":"10.1016\/j.vaccine.2013.04.012_bib0065","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.coi.2011.06.001","article-title":"Shigella are versatile mucosal pathogens that circumvent the host innate immune system","volume":"23","author":"Ashida","year":"2011","journal-title":"Curr Opin Immunol"},{"issue":"January, 2","key":"10.1016\/j.vaccine.2013.04.012_bib0070","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1021\/bi101365f","article-title":"Conformational changes in IpaD from Shigella flexneri upon binding bile salts provide insight into the second step of type III secretion","volume":"50","author":"Dickenson","year":"2011","journal-title":"Biochemistry"},{"issue":"July, 27","key":"10.1016\/j.vaccine.2013.04.012_bib0075","doi-asserted-by":"crossref","first-page":"18646","DOI":"10.1074\/jbc.M802799200","article-title":"Deoxycholate interacts with IpaD of Shigella flexneri in inducing the recruitment of IpaB to the type III secretion apparatus needle tip","volume":"283","author":"Stensrud","year":"2008","journal-title":"J Biol Chem"},{"issue":"November, 3","key":"10.1016\/j.vaccine.2013.04.012_bib0080","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1083\/jcb.147.3.683","article-title":"The tripartite type III secreton of Shigella flexneri inserts IpaB and IpaC into host membranes","volume":"147","author":"Blocker","year":"1999","journal-title":"J Cell Biol"},{"issue":"March, 3","key":"10.1016\/j.vaccine.2013.04.012_bib0085","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1128\/IAI.06174-11","article-title":"Broadly protective Shigella vaccine based on type III secretion apparatus proteins","volume":"80","author":"Martinez-Becerra","year":"2012","journal-title":"Infect Immun"},{"issue":"January, 1","key":"10.1016\/j.vaccine.2013.04.012_bib0090","doi-asserted-by":"crossref","first-page":"76","DOI":"10.4161\/hv.8.1.18809","article-title":"Live attenuated intranasal influenza vaccine","volume":"8","author":"Esposito","year":"2012","journal-title":"Hum Vaccine Immunother"},{"issue":"9","key":"10.1016\/j.vaccine.2013.04.012_bib0095","doi-asserted-by":"crossref","first-page":"e6999","DOI":"10.1371\/journal.pone.0006999","article-title":"Transient facial nerve paralysis (Bell's palsy) following intranasal delivery of a genetically detoxified mutant of Escherichia coli heat labile toxin","volume":"4","author":"Lewis","year":"2009","journal-title":"PloS one"},{"issue":"September, 18","key":"10.1016\/j.vaccine.2013.04.012_bib0100","doi-asserted-by":"crossref","first-page":"10078","DOI":"10.1128\/JVI.77.18.10078-10087.2003","article-title":"Enhanced mucosal immunoglobulin A response of intranasal adenoviral vector human immunodeficiency virus vaccine and localization in the central nervous system","volume":"77","author":"Lemiale","year":"2003","journal-title":"J Virol"},{"issue":"November, 9","key":"10.1016\/j.vaccine.2013.04.012_bib0105","doi-asserted-by":"crossref","first-page":"1628","DOI":"10.1086\/491739","article-title":"Proinflammatory responses in the murine brain after intranasal delivery of cholera toxin: implications for the use of AB toxins as adjuvants in intranasal vaccines","volume":"192","author":"Armstrong","year":"2005","journal-title":"J Infect Dis"},{"issue":"August, 33\u201334","key":"10.1016\/j.vaccine.2013.04.012_bib0110","doi-asserted-by":"crossref","first-page":"5937","DOI":"10.1016\/j.vaccine.2006.06.005","article-title":"Enhanced humoral and memory B cellular immunity using HPV16\/18 L1 VLP vaccine formulated with the MPL\/aluminium salt combination (AS04) compared to aluminium salt only","volume":"24","author":"Giannini","year":"2006","journal-title":"Vaccine"},{"issue":"October, 20","key":"10.1016\/j.vaccine.2013.04.012_bib0115","doi-asserted-by":"crossref","first-page":"3231","DOI":"10.1007\/s00018-008-8228-6","article-title":"Putting endotoxin to work for us: monophosphoryl lipid A as a safe and effective vaccine adjuvant","volume":"65","author":"Casella","year":"2008","journal-title":"Cell Mol Life Sci: CMLS"},{"issue":"April, 14","key":"10.1016\/j.vaccine.2013.04.012_bib0120","doi-asserted-by":"crossref","first-page":"13406","DOI":"10.1074\/jbc.M500687200","article-title":"Effects of adsorption to aluminum salt adjuvants on the structure and stability of model protein antigens","volume":"280","author":"Jones","year":"2005","journal-title":"J Biol Chem"},{"issue":"November, 10","key":"10.1016\/j.vaccine.2013.04.012_bib0125","doi-asserted-by":"crossref","first-page":"6186","DOI":"10.4049\/jimmunol.0901474","article-title":"AS04, an aluminum salt- and TLR4 agonist-based adjuvant system, induces a transient localized innate immune response leading to enhanced adaptive immunity","volume":"183","author":"Didierlaurent","year":"2009","journal-title":"J Immunol"},{"issue":"May, 19","key":"10.1016\/j.vaccine.2013.04.012_bib0130","doi-asserted-by":"crossref","first-page":"7914","DOI":"10.1073\/pnas.1104588108","article-title":"Vaccine adjuvants aluminum and monophosphoryl lipid A provide distinct signals to generate protective cytotoxic memory CD8 T cells","volume":"108","author":"MacLeod","year":"2011","journal-title":"Proc Natl Acad Sci USA"},{"issue":"July, 7","key":"10.1016\/j.vaccine.2013.04.012_bib0135","doi-asserted-by":"crossref","first-page":"2426","DOI":"10.1128\/IAI.00181-12","article-title":"A subunit of Escherichia coli heat-labile enterotoxin functions as a mucosal adjuvant and promotes IgG2a, IgA, and Th17 responses to vaccine antigens","volume":"80","author":"Norton","year":"2012","journal-title":"Infect Immun"},{"issue":"January, 1","key":"10.1016\/j.vaccine.2013.04.012_bib0140","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/0003-2697(92)90279-G","article-title":"Statistical determination of the average values of the extinction coefficients of tryptophan and tyrosine in native proteins","volume":"200","author":"Mach","year":"1992","journal-title":"Anal Biochem"},{"issue":"September, 9","key":"10.1016\/j.vaccine.2013.04.012_bib0145","doi-asserted-by":"crossref","first-page":"5230","DOI":"10.1128\/IAI.69.9.5230-5234.2001","article-title":"Predominance of serotype-specific mucosal antibody response in Shigella flexneri-infected humans living in an area of endemicity","volume":"69","author":"Rasolofo-Razanamparany","year":"2001","journal-title":"Infect Immun"},{"issue":"September, 6","key":"10.1016\/j.vaccine.2013.04.012_bib0150","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1093\/oxfordjournals.aje.a116134","article-title":"Epidemiologic patterns of acute diarrhea and endemic Shigella infections in children in a poor periurban setting in Santiago, Chile","volume":"134","author":"Ferreccio","year":"1991","journal-title":"Am J Epidemiol"},{"issue":"September, 3","key":"10.1016\/j.vaccine.2013.04.012_bib0155","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1093\/infdis\/164.3.533","article-title":"Effect of prior infection with virulent Shigella flexneri 2a on the resistance of monkeys to subsequent infection with Shigella sonnei","volume":"164","author":"Formal","year":"1991","journal-title":"J Infect Dis"},{"issue":"March, 13","key":"10.1016\/j.vaccine.2013.04.012_bib0160","doi-asserted-by":"crossref","first-page":"2290","DOI":"10.1016\/j.vaccine.2005.11.040","article-title":"Development and evaluation of a Shigella flexneri 2a and S. sonnei bivalent invasin complex (Invaplex) vaccine","volume":"24","author":"Oaks","year":"2006","journal-title":"Vaccine"},{"issue":"June, 6","key":"10.1016\/j.vaccine.2013.04.012_bib0165","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1006\/mpat.1996.0127","article-title":"Construction and characterization of genetically-marked bivalent anti-Shigella dysenteriae 1 and anti-Shigella flexneri Y live vaccine candidates","volume":"22","author":"Klee","year":"1997","journal-title":"Microb Pathog"},{"issue":"April, 3","key":"10.1016\/j.vaccine.2013.04.012_bib0170","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1111\/j.1574-695X.2006.00062.x","article-title":"Construction of a multivalent vaccine strain of Shigella flexneri and evaluation of serotype-specific immunity","volume":"46","author":"Jennison","year":"2006","journal-title":"FEMS Immunol Med Microbiol"},{"issue":"February, 4","key":"10.1016\/j.vaccine.2013.04.012_bib0175","doi-asserted-by":"crossref","first-page":"2076","DOI":"10.4049\/jimmunol.0900978","article-title":"Th17 cells are the dominant T cell subtype primed by Shigella flexneri mediating protective immunity","volume":"184","author":"Sellge","year":"2010","journal-title":"J Immunol"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X13004313?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X13004313?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,10,16]],"date-time":"2018-10-16T22:25:03Z","timestamp":1539728703000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X13004313"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5]]},"references-count":35,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2013,5]]}},"alternative-id":["S0264410X13004313"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2013.04.012","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2013,5]]}}}