{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:35:16Z","timestamp":1788910516311,"version":"build-2803163510"},"reference-count":29,"publisher":"Elsevier BV","issue":"48","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2013,11]]},"DOI":"10.1016\/j.vaccine.2013.09.033","type":"journal-article","created":{"date-parts":[[2013,9,30]],"date-time":"2013-09-30T12:15:57Z","timestamp":1380543357000},"page":"5729-5735","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":10,"title":["Intranasal immunization with a non-adjuvanted adhesive protein descended from Pasteurella pneumotropica and its preventive efficacy against opportunistic infection in mice"],"prefix":"10.1016","volume":"31","author":[{"given":"Hiraku","family":"Sasaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Itoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Hosomi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiichi","family":"Kawamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2013.09.033_bib0005","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/S0169-409X(01)00162-4","article-title":"Nasal vaccines","volume":"51","author":"Davis","year":"2001","journal-title":"Adv Drug Deliv Rev"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0010","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/S1461-5347(00)00281-9","article-title":"Intranasal vaccines: forthcoming challenges","volume":"3","author":"Partidos","year":"2000","journal-title":"Pharm Sci Technol Today"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0015","doi-asserted-by":"crossref","first-page":"2708","DOI":"10.1016\/S0264-410X(00)00507-7","article-title":"The bare skin and the nose as non-invasive routes for administering peptide vaccines","volume":"19","author":"Partidos","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0020","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1038\/36014","article-title":"Skin immunization made possible by cholera toxin","volume":"391","author":"Glenn","year":"1998","journal-title":"Nature"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0025","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.1128\/IAI.67.3.1100-1106.1999","article-title":"Transcutaneous immunization with bacterial ADP-ribosylating exotoxins as antigens and adjuvants","volume":"67","author":"Glenn","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0030","doi-asserted-by":"crossref","first-page":"5306","DOI":"10.1128\/IAI.68.9.5306-5313.2000","article-title":"Transcutaneous immunization with bacterial ADP-ribosylating exotoxins, subunits, and unrelated adjuvants","volume":"68","author":"Scharton-Kersten","year":"2000","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0035","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0264-410X(93)90190-9","article-title":"Adjuvants \u2013 a balance between toxicity and adjuvanticity","volume":"11","author":"Gupta","year":"1993","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0040","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1051\/vetres:2006014","article-title":"Adjuvants modulating mucosal immune responses or directing systemic responses towards the mucosa","volume":"37","author":"Cox","year":"2006","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0045","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1586\/erv.10.142","article-title":"Novel adjuvants and delivery systems for enhancing immune responses induced by immunogens","volume":"10","author":"Dey","year":"2011","journal-title":"Expert Rev Vaccines"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0050","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1051\/vetres:2006012","article-title":"Mucosal delivery of vaccines in domestic animals","volume":"37","author":"Gerdts","year":"2006","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0055","doi-asserted-by":"crossref","first-page":"e1003001","DOI":"10.1371\/journal.ppat.1003001","article-title":"Vaccines: from empirical development to rational design","volume":"8","author":"Rueckert","year":"2012","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0060","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1021\/mp060098+","article-title":"Vaccine delivery methods using viral vectors","volume":"4","author":"Brave","year":"2007","journal-title":"Mol Pharm"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0065","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.immuni.2010.10.004","article-title":"Immunologic basis of vaccine vectors","volume":"33","author":"Liu","year":"2010","journal-title":"Immunity"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0070","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1538\/expanim.60.463","article-title":"Pathogenicity of Pasteurella pneumotropica in immunodeficient NOD\/ShiJic-scid\/Jcl and immunocompetent Crlj:CD1 (ICR) mice","volume":"60","author":"Kawamoto","year":"2011","journal-title":"Exp Anim"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0075","doi-asserted-by":"crossref","first-page":"3698","DOI":"10.1128\/JB.01527-08","article-title":"Identification and characterization of hemolysin-like proteins similar to RTX toxin in Pasteurella pneumotropica","volume":"191","author":"Sasaki","year":"2009","journal-title":"J Bacteriol"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0080","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1186\/1471-2180-11-55","article-title":"Molecular and virulence characteristics of an outer membrane-associated RTX exoprotein in Pasteurella pneumotropica","volume":"11","author":"Sasaki","year":"2011","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0085","first-page":"161","article-title":"The leukotoxin of Pasteurella haemolytica binds to beta2 integrins on bovine leukocytes","volume":"179","author":"Ambagala","year":"1999","journal-title":"FEMS Microbiol Lett"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0090","doi-asserted-by":"crossref","first-page":"30463","DOI":"10.1074\/jbc.272.48.30463","article-title":"RTX toxins recognize a \u03b22 integrin on the surface of human target cells","volume":"272","author":"Lally","year":"1997","journal-title":"J Biol Chem"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0095","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1128\/IAI.68.1.72-79.2000","article-title":"Lymphocyte function-associated antigen 1 is a receptor for Pasteurella haemolytica leukotoxin in bovine leukocytes","volume":"68","author":"Jeyaseelan","year":"2000","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0100","doi-asserted-by":"crossref","first-page":"1076","DOI":"10.1111\/j.1574-6976.2010.00231.x","article-title":"RTX proteins: a highly diverse family secreted by a common mechanism","volume":"34","author":"Linhartov\u00e1","year":"2010","journal-title":"FEMS Microbiol Rev"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0105","doi-asserted-by":"crossref","first-page":"2818","DOI":"10.1128\/iai.63.8.2818-2825.1995","article-title":"Clearance of Pseudomonas aeruginosa from the murine gastrointestinal tract is effectively mediated by O-antigen-specific circulating antibodies","volume":"63","author":"Pier","year":"1995","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0110","doi-asserted-by":"crossref","first-page":"4624","DOI":"10.1073\/pnas.0608657104","article-title":"Intranasal immunization with heterologously expressed polysaccharide protects against multiple Pseudomonas aeruginosa infections","volume":"104","author":"DiGiandomenico","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0115","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1586\/erv.11.196","article-title":"Novel vaccine development strategies for inducing mucosal immunity","volume":"11","author":"Fujkuyama","year":"2012","journal-title":"Expert Rev Vaccines"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0120","doi-asserted-by":"crossref","first-page":"10986","DOI":"10.1073\/pnas.0703766104","article-title":"Rice-based mucosal vaccine as a global strategy for cold-chain- and needle-free vaccination","volume":"104","author":"Nochi","year":"2007","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0125","doi-asserted-by":"crossref","first-page":"8794","DOI":"10.1073\/pnas.0914121107","article-title":"Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine","volume":"107","author":"Tokuhara","year":"2010","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0130","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1038\/nmat2784","article-title":"Nanogel antigenic protein-delivery system for adjuvant-free intranasal vaccines","volume":"9","author":"Nochi","year":"2010","journal-title":"Nat Mater"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0135","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/0264-410X(95)93129-W","article-title":"Mucosal immunoadjuvant activity of liposomes, induction of systemic IgG and secretory IgA responses in mice by intranasal immunization with an influenza subunit vaccine and coadministered liposomes","volume":"13","author":"de Haan","year":"1995","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0140","doi-asserted-by":"crossref","first-page":"1320","DOI":"10.1016\/0264-410X(95)00037-2","article-title":"Liposomes as an immunoadjuvant system for stimulation of mucosal and systemic antibody responses against inactivated measles virus administered intranasally to mice","volume":"13","author":"de Haan","year":"1995","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2013.09.033_bib0145","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.vaccine.2006.06.086","article-title":"N-trimethyl chitosan (TMC) nanoparticles loaded with influenza subunit antigen for intranasal vaccination: biological properties and immunogenicity in a mouse model","volume":"25","author":"Amidi","year":"2007","journal-title":"Vaccine"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X13012747?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X13012747?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:19:50Z","timestamp":1759400390000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X13012747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11]]},"references-count":29,"journal-issue":{"issue":"48","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["S0264410X13012747"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2013.09.033","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2013,11]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Intranasal immunization with a non-adjuvanted adhesive protein descended from Pasteurella pneumotropica and its preventive efficacy against opportunistic infection in mice","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2013.09.033","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2013 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}