{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T04:37:12Z","timestamp":1786423032106,"version":"build-2736575974"},"reference-count":49,"publisher":"Elsevier BV","issue":"18","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2010CB530200\/2010CB530205"],"award-info":[{"award-number":["2010CB530200\/2010CB530205"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81371767\/31170161"],"award-info":[{"award-number":["81371767\/31170161"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Natural Science and Technology Major Project of China","award":["2013ZX10004803"],"award-info":[{"award-number":["2013ZX10004803"]}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2014,4]]},"DOI":"10.1016\/j.vaccine.2014.02.057","type":"journal-article","created":{"date-parts":[[2014,2,28]],"date-time":"2014-02-28T11:30:58Z","timestamp":1393587058000},"page":"2027-2033","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":29,"title":["Surface protein Adr2 of Rickettsia rickettsii induced protective immunity against Rocky Mountain spotted fever in C3H\/HeN mice"],"prefix":"10.1016","volume":"32","author":[{"given":"Wenping","family":"Gong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolu","family":"Xiong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Jiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changsong","family":"Duan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bohai","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2014.02.057_bib0005","series-title":"The so-called spotted fever of the Rocky Mountains \u2013 a new disease in Bitter Root Valley, Mont. Public Health Reports (1896\u20131970)","first-page":"1868","author":"Cobb","year":"1902"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0010","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1093\/infdis\/1.1.31","article-title":"Studies in Pyroplasmosis hominis (spotted fever or tick fever of the Rocky Mountains) (with map, charts, and plates I, II)","volume":"1","author":"Wilson","year":"1904","journal-title":"Journal of Infectious Diseases"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0015","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1603\/ME10181","article-title":"Rickettsia rickettsii in Rhipicephalus ticks, Mexicali, Mexico","volume":"48","author":"Eremeeva","year":"2011","journal-title":"Journal of Medical Entomology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0020","doi-asserted-by":"crossref","first-page":"713","DOI":"10.4269\/ajtmh.2012.11-0453","article-title":"Fatal Rocky Mountain Spotted Fever in the United States, 1999\u20132007","volume":"86","author":"Dahlgren","year":"2012","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0025","series-title":"Epidemiology of Brazilian spotted fever in the Atlantic Forest, state of S\u00e3o Paulo, Brazil","author":"Labruna","year":"2012"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.ttbdis.2012.10.010","article-title":"Features of Brazilian spotted fever in two different endemic areas in Brazil","author":"Angerami","year":"2012","journal-title":"Ticks and Tick-Borne Diseases"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0035","doi-asserted-by":"crossref","first-page":"369","DOI":"10.4269\/ajtmh.1941.s1-21.369","article-title":"Rocky Mountain spotted fever: results of fifteen years\u2019 prophylactic vaccination","volume":"1","author":"Parker","year":"1941","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0040","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1126\/science.94.2444.399","article-title":"Cultivation of Rickettsiae of the Rocky Mountain spotted fever, typhus and Q fever groups in the embryonic tissues of developing chicks","volume":"94","author":"Cox","year":"1941","journal-title":"Science (New York, NY)"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0045","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1128\/JCM.1.6.500-503.1975","article-title":"Preparation of Rocky Mountain spotted fever vaccine suitable for human immunization","volume":"1","author":"Kenyon","year":"1975","journal-title":"Journal of Clinical Microbiology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0050","doi-asserted-by":"crossref","first-page":"371","DOI":"10.4269\/ajtmh.2001.65.371","article-title":"Identification of protective components of two major outer membrane proteins of spotted fever group Rickettsiae","volume":"65","author":"Diaz-Montero","year":"2001","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0055","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1093\/infdis\/128.3.340","article-title":"Rocky Mountain spotted fever: a comparative study of the active immunity induced by inactivated and viable pathogenic Rickettsia rickettsii","volume":"128","author":"DuPont","year":"1973","journal-title":"Journal of Infectious Diseases"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0060","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1128\/IAI.25.2.580-582.1979","article-title":"Exposure of guinea pigs to Rickettsia rickettsii by aerosol, nasal, conjunctival, gastric, and subcutaneous routes and protection afforded by an experimental vaccine","volume":"25","author":"Kenyon","year":"1979","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0065","doi-asserted-by":"crossref","first-page":"859","DOI":"10.1056\/NEJM197710202971604","article-title":"Laboratory-acquired Rocky Mountain spotted fever: the hazard of aerosol transmission","volume":"297","author":"Oster","year":"1977","journal-title":"New England Journal of Medicine"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0070","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1006\/mpat.1997.0197","article-title":"rOmpA is a critical protein for the adhesion of Rickettsia rickettsii to host cells","volume":"24","author":"Li","year":"1998","journal-title":"Microbial Pathogenesis"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0075","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1016\/j.micinf.2005.10.003","article-title":"The major outer membrane protein rOmpB of spotted fever group rickettsiae functions in the rickettsial adherence to and invasion of Vero cells","volume":"8","author":"Uchiyama","year":"2006","journal-title":"Microbes and Infection"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0080","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1128\/IAI.01165-09","article-title":"The Rickettsia conorii autotransporter protein Sca1 promotes adherence to nonphagocytic mammalian cells","volume":"78","author":"Riley","year":"2010","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0085","doi-asserted-by":"crossref","first-page":"5272","DOI":"10.1128\/IAI.00201-09","article-title":"The Sca2 autotransporter protein from Rickettsia conorii is sufficient to mediate adherence to and invasion of cultured mammalian cells","volume":"77","author":"Cardwell","year":"2009","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0090","doi-asserted-by":"crossref","first-page":"605","DOI":"10.1016\/j.resmic.2006.02.002","article-title":"Identification of two putative rickettsial adhesins by proteomic analysis","volume":"157","author":"Renesto","year":"2006","journal-title":"Research in Microbiology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0095","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1016\/j.micpath.2011.01.009","article-title":"Characterization of rickettsial adhesin Adr2 belonging to a new group of adhesins in \u03b1-proteobacteria","volume":"50","author":"Vellaiswamy","year":"2011","journal-title":"Microbial Pathogenesis"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0100","doi-asserted-by":"crossref","first-page":"2760","DOI":"10.1128\/IAI.58.9.2760-2769.1990","article-title":"A protective protein antigen of Rickettsia rickettsii has tandemly repeated, near-identical sequences","volume":"58","author":"Anderson","year":"1990","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0105","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1128\/IAI.60.1.159-165.1992","article-title":"Evidence for proteolytic cleavage of the 120-kilodalton outer membrane protein of rickettsiae: identification of an avirulent mutant deficient in processing","volume":"60","author":"Hackstadt","year":"1992","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.vaccine.2013.09.064","article-title":"Recombinant protein YbgF induces protective immunity against Rickettsia heilongjiangensis infection in C3H\/HeN mice","author":"Qi","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0115","doi-asserted-by":"crossref","DOI":"10.1002\/9780471729259.mc03a05s11","article-title":"Laboratory maintenance of Rickettsia rickettsii","author":"Ammerman","year":"2008","journal-title":"Current Protocols in Microbiology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0120","doi-asserted-by":"crossref","first-page":"e70440","DOI":"10.1371\/journal.pone.0070440","article-title":"Proteome analysis and serological characterization of surface-exposed proteins of Rickettsia heilongjiangensis","volume":"8","author":"Qi","year":"2013","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0125","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1186\/1471-2180-12-35","article-title":"Potential serodiagnostic markers for Q fever identified in Coxiella burnetii by immunoproteomic and protein microarray approaches","volume":"12","author":"Xiong","year":"2012","journal-title":"BMC Microbiology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0130","first-page":"526","article-title":"Development of quantitative PCR specific for Ricekttsia rickettsii","volume":"6","author":"Niu","year":"2006","journal-title":"Chinese Journal of Epidemiology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0135","doi-asserted-by":"crossref","DOI":"10.1002\/0471142735.im0301s39","article-title":"Isolation of mouse mononuclear cells","author":"Kruisbeek","year":"2001","journal-title":"Current Protocols in Immunology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0140","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1126\/science.3099387","article-title":"Cloned gene of Rickettsia rickettsii surface antigen: candidate vaccine for Rocky Mountain spotted fever","volume":"235","author":"McDonald","year":"1987","journal-title":"Science"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0145","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1016\/S0264-410X(01)00377-2","article-title":"Immunization with a portion of rickettsial outer membrane protein A stimulates protective immunity against spotted fever rickettsiosis","volume":"20","author":"Crocquet-Valdes","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0150","doi-asserted-by":"crossref","first-page":"2222","DOI":"10.1128\/IAI.72.4.2222-2228.2004","article-title":"Fc-dependent polyclonal antibodies and antibodies to outer membrane proteins A and B, but not to lipopolysaccharide, protect SCID mice against fatal Rickettsia conorii infection","volume":"72","author":"Feng","year":"2004","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0155","doi-asserted-by":"crossref","first-page":"2303","DOI":"10.1128\/IAI.01324-10","article-title":"Molecular basis of immunity to rickettsial infection conferred through outer membrane protein B","volume":"79","author":"Chan","year":"2011","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0160","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1093\/infdis\/158.1.228","article-title":"Protection of guinea pigs from experimental Rocky Mountain spotted fever with a cloned antigen of Rickettsia rickettsii","volume":"158","author":"Mcdonald","year":"1988","journal-title":"Journal of Infectious Diseases"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0165","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1002\/eji.1830111212","article-title":"Activation of mouse complement by monoclonal mouse antibodies","volume":"11","author":"Neuberger","year":"1981","journal-title":"European Journal of Immunology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0170","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1007\/s002770050396","article-title":"The IgG Fc receptor family","volume":"76","author":"Gessner","year":"1998","journal-title":"Annals of Hematology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0175","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1084\/jem.142.6.1520","article-title":"Binding of monomeric immunoglobulins to Fc receptors of mouse macrophages","volume":"142","author":"Unkeless","year":"1975","journal-title":"Journal of Experimental Medicine"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0180","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1084\/jem.161.1.1","article-title":"Importance of immunoglobulin isotype in human antibody-dependent, cell-mediated cytotoxicity directed by murine monoclonal antibodies","volume":"161","author":"Kipps","year":"1985","journal-title":"Journal of Experimental Medicine"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0185","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1016\/0092-8674(94)90115-5","article-title":"FcR \u03b3-chain deletion results in pleiotrophic effector cell defects","volume":"76","author":"Takai","year":"1994","journal-title":"Cell"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0190","doi-asserted-by":"crossref","first-page":"3524","DOI":"10.1128\/IAI.72.6.3524-3530.2004","article-title":"Effect of antibody on the rickettsia\u2013host cell interaction","volume":"72","author":"Feng","year":"2004","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0195","doi-asserted-by":"crossref","first-page":"6729","DOI":"10.1128\/IAI.68.12.6729-6736.2000","article-title":"Mechanisms of intracellular killing of Rickettsia conorii in infected human endothelial cells, hepatocytes, and macrophages","volume":"68","author":"Feng","year":"2000","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0200","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1128\/CVI.00156-06","article-title":"Distinct contributions of vaccine-induced immunoglobulin G1 (IgG1) and IgG2a antibodies to protective immunity against influenza","volume":"13","author":"Huber","year":"2006","journal-title":"Clinical and Vaccine Immunology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0205","doi-asserted-by":"crossref","first-page":"832","DOI":"10.1128\/IAI.51.3.832-837.1986","article-title":"Cross-reactive lymphocyte responses and protective immunity against other spotted fever group rickettsiae in mice immunized with Rickettsia conorii","volume":"51","author":"Jerrells","year":"1986","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0210","doi-asserted-by":"crossref","first-page":"5314","DOI":"10.4049\/jimmunol.158.11.5314","article-title":"Role of T lymphocyte subsets in immunity to spotted fever group Rickettsiae","volume":"158","author":"Feng H-m Popov","year":"1997","journal-title":"Journal of Immunology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0215","doi-asserted-by":"crossref","first-page":"2018","DOI":"10.1128\/CVI.05292-11","article-title":"Immunization with Ehrlichia P28 outer membrane proteins confers protection in a mouse model of Ehrlichiosis","volume":"18","author":"Crocquet-Valdes","year":"2011","journal-title":"Clinical and Vaccine Immunology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0220","doi-asserted-by":"crossref","first-page":"1841","DOI":"10.1128\/IAI.69.3.1841-1846.2001","article-title":"Critical role of cytotoxic T lymphocytes in immune clearance of rickettsial infection","volume":"69","author":"Walker","year":"2001","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0225","doi-asserted-by":"crossref","first-page":"4609","DOI":"10.1128\/IAI.00563-08","article-title":"Enumeration of cytotoxic CD8T cells ex vivo during the response to Listeria monocytogenes infection","volume":"76","author":"Zaiss","year":"2008","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0230","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1111\/j.1365-2567.2006.02452.x","article-title":"CD4+ Th1 cells promote CD8+ Tc1 cell survival, memory response, tumor localization and therapy by targeted delivery of interleukin 2 via acquired pMHC I complexes","volume":"120","author":"Huang","year":"2007","journal-title":"Immunology"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0235","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1128\/IAI.62.5.1952-1960.1994","article-title":"Depletion of gamma interferon and tumor necrosis factor alpha in mice with Rickettsia conorii-infected endothelium: impairment of rickettsicidal nitric oxide production resulting in fatal, overwhelming rickettsial disease","volume":"62","author":"Feng","year":"1994","journal-title":"Infection and Immunity"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0240","doi-asserted-by":"crossref","first-page":"770","DOI":"10.1086\/519739","article-title":"Intralesional expression of mRNA of interferon-\u03b3, tumor necrosis factor-\u03b1, interleukin-10, nitric oxide synthase, indoleamine-2, 3-dioxygenase, and RANTES is a major immune effector in Mediterranean spotted fever rickettsiosis","volume":"196","author":"de Sousa","year":"2007","journal-title":"Journal of Infectious Diseases"},{"key":"10.1016\/j.vaccine.2014.02.057_bib0245","first-page":"1016","article-title":"Interferon-gamma and tumor necrosis factor-alpha exert their antirickettsial effect via induction of synthesis of nitric oxide","volume":"143","author":"Feng","year":"1993","journal-title":"American Journal of Pathology"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X14002424?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X14002424?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,7,10]],"date-time":"2023-07-10T21:08:33Z","timestamp":1689023313000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X14002424"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":49,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["S0264410X14002424"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2014.02.057","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Surface protein Adr2 of Rickettsia rickettsii induced protective immunity against Rocky Mountain spotted fever in C3H\/HeN mice","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2014.02.057","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}