{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T20:55:50Z","timestamp":1769547350310,"version":"3.49.0"},"reference-count":50,"publisher":"Elsevier BV","issue":"33","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31070819"],"award-info":[{"award-number":["31070819"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chongqing Key Laboratory (CSTC)"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2014,7]]},"DOI":"10.1016\/j.vaccine.2014.05.019","type":"journal-article","created":{"date-parts":[[2014,6,2]],"date-time":"2014-06-02T22:15:47Z","timestamp":1401747347000},"page":"4179-4188","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":23,"title":["Mucosal and systemic immunization with a novel attenuated pneumococcal vaccine candidate confer serotype independent protection against Streptococcus pneumoniae in mice"],"prefix":"10.1016","volume":"32","author":[{"given":"Kaifeng","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Run","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Pang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuai","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuemei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibing","family":"Yin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2014.05.019_bib0005","unstructured":"Organization WH. Global immunization data; 2008. p. 2."},{"key":"10.1016\/j.vaccine.2014.05.019_bib0010","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1053\/jinf.2000.0653","article-title":"Early predictors of mortality in pneumococcal bacteraemia","volume":"40","author":"Balakrishnan","year":"2000","journal-title":"J Infect"},{"issue":"Suppl. 2","key":"10.1016\/j.vaccine.2014.05.019_bib0015","doi-asserted-by":"crossref","first-page":"S97","DOI":"10.1086\/596487","article-title":"Use of multiple surveillance modalities to assess the epidemiology of Streptococcus pneumoniae infection in Bangladesh","volume":"48","author":"Luby","year":"2009","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0020","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1086\/374052","article-title":"Factors associated with relative rates of antimicrobial resistance among Streptococcus pneumoniae in the United States: results from the TRUST Surveillance Program (1998\u20132002)","volume":"36","author":"Karlowsky","year":"2003","journal-title":"Clin Infect Dis"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0025","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/S0732-8893(02)00522-9","article-title":"Antimicrobial susceptibility of community-acquired respiratory tract pathogens in North America in 1999\u20132000: findings of the PROTEKT surveillance study","volume":"45","author":"Hoban","year":"2003","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0030","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.1128\/AAC.48.6.2101-2107.2004","article-title":"High prevalence of antimicrobial resistance among clinical Streptococcus pneumoniae isolates in Asia (an ANSORP Study)","volume":"48","author":"Song","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0035","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1016\/j.diagmicrobio.2008.02.004","article-title":"Serotype distribution and antimicrobial resistance patterns of Streptococcus pneumoniae isolated from children in China younger than 5 years","volume":"61","author":"Liu","year":"2008","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0040","first-page":"175","article-title":"Rate of nasopharyngeal carriage, antimicrobial resistance and serotype of Streptococcus pneumoniae among children in northern Taiwan","volume":"36","author":"Lo","year":"2003","journal-title":"J Microbiol Immunol Infect"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0045","doi-asserted-by":"crossref","first-page":"2765","DOI":"10.1128\/AAC.45.10.2765-2770.2001","article-title":"Nasopharyngeal carriage of antimicrobial-resistant Streptococcus pneumoniae among young children attending 79 kindergartens and day care centers in Hong Kong","volume":"45","author":"Chiu","year":"2001","journal-title":"Antimicrob Agents Chemother"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0050","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1186\/1471-2180-12-228","article-title":"Molecular characteristics of erythromycin-resistant Streptococcus pneumoniae from pediatric patients younger than five years in Beijing, 2010","volume":"12","author":"Zhou","year":"2012","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0055","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/21202","article-title":"Emergence of vancomycin tolerance in Streptococcus pneumoniae","volume":"399","author":"Novak","year":"1999","journal-title":"Nature"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0060","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1056\/NEJM199111213252101","article-title":"The protective efficacy of polyvalent pneumococcal polysaccharide vaccine","volume":"325","author":"Shapiro","year":"1991","journal-title":"New Engl J Med"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0065","first-page":"3","article-title":"Streptococcus pneumoniae: epidemiology and patterns of resistance","volume":"117","author":"Jacobs","year":"2004","journal-title":"Am J Med"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0070","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1086\/653123","article-title":"A new pneumococcal serotype, 11E, has a variably inactivated wcjE gene","volume":"202","author":"Calix","year":"2010","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0075","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1084\/jem.20071168","article-title":"Discovery of a novel class of highly conserved vaccine antigens using genomic scale antigenic fingerprinting of pneumococcus with human antibodies","volume":"205","author":"Giefing","year":"2008","journal-title":"J Exp Med"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0080","doi-asserted-by":"crossref","first-page":"2398","DOI":"10.1016\/j.vaccine.2006.09.008","article-title":"The preventable burden of pneumococcal disease in the developing world","volume":"25","author":"Scott","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0085","doi-asserted-by":"crossref","first-page":"2189","DOI":"10.1016\/S0140-6736(03)13772-5","article-title":"Effect of conjugate pneumococcal vaccine followed by polysaccharide pneumococcal vaccine on recurrent acute otitis media: a randomised study","volume":"361","author":"Veenhoven","year":"2003","journal-title":"Lancet"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0090","doi-asserted-by":"crossref","first-page":"1871","DOI":"10.1016\/S0140-6736(04)16357-5","article-title":"Colonisation by Streptococcus pneumoniae and Staphylococcus aureus in healthy children","volume":"363","author":"Bogaert","year":"2004","journal-title":"Lancet"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0095","doi-asserted-by":"crossref","first-page":"5289","DOI":"10.1016\/j.vaccine.2013.08.025","article-title":"Safety of 13-valent pneumococcal conjugate vaccine in infants and children: meta-analysis of 13 clinical trials in 9 countries","volume":"31","author":"Thompson","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0100","doi-asserted-by":"crossref","first-page":"2469","DOI":"10.1128\/IAI.01972-06","article-title":"Live attenuated Streptococcus pneumoniae strains induce serotype-independent mucosal and systemic protection in mice","volume":"75","author":"Roche","year":"2007","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0105","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0928-8244(03)00146-9","article-title":"Novel vaccine strategies with protein antigens of Streptococcus pneumoniae","volume":"38","author":"Swiatlo","year":"2003","journal-title":"FEMS Immunol Med Microbiol"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0110","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1086\/599839","article-title":"A live-attenuated vaccine for the treatment of urinary tract infection by uropathogenic Escherichia coli","volume":"200","author":"Billips","year":"2009","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0115","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1128\/IAI.31.1.160-168.1981","article-title":"Nature of the cross-protective antigen in subcellular vaccines of Streptococcus pneumoniae","volume":"31","author":"Au","year":"1981","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0120","doi-asserted-by":"crossref","first-page":"2008","DOI":"10.1016\/j.vaccine.2011.11.073","article-title":"Streptococcus pneumoniae pep27 mutant as a live vaccine for serotype-independent protection in mice","volume":"30","author":"Kim","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0125","article-title":"A live-attenuated pneumococcal vaccine elicits CD4+ T-cell dependent class switching and provides serotype independent protection against acute otitis media","author":"Rosch","year":"2013","journal-title":"EMBO Mol Med"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0130","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1128\/IAI.00473-09","article-title":"Immunization with a combination of three pneumococcal proteins confers additive and broad protection against Streptococcus pneumoniae Infections in Mice","volume":"78","author":"Wu","year":"2010","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0135","doi-asserted-by":"crossref","first-page":"1187","DOI":"10.1139\/W09-078","article-title":"Contribution of ClpE to virulence of Streptococcus pneumoniae","volume":"55","author":"Zhang","year":"2009","journal-title":"Can J Microbiol"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0140","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1046\/j.1365-2958.1999.01291.x","article-title":"Pneumococcal licD2 gene is involved in phosphorylcholine metabolism","volume":"31","author":"Zhang","year":"1999","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0145","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1111\/j.1365-2958.1994.tb01076.x","article-title":"Peptide permeases modulate transformation in Streptococcus pneumoniae","volume":"12","author":"Pearce","year":"1994","journal-title":"Mol Microbiol"},{"issue":"12","key":"10.1016\/j.vaccine.2014.05.019_bib0150","first-page":"1","article-title":"Bone marrow-derived macrophages (BMM): isolation and applications","volume":"3","author":"Weischenfeldt","year":"2008","journal-title":"Cold Spring Harb Protoc"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0155","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1086\/513980","article-title":"Intranasal immunization of mice with PspA (Pneumococcal Surface Protein A) can prevent intranasal carriage, pulmonary infection, and sepsis with Streptococcus pneumoniae","volume":"175","author":"Wu","year":"1997","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0160","doi-asserted-by":"crossref","first-page":"4996","DOI":"10.1016\/j.vaccine.2007.04.069","article-title":"Enhanced protection against pneumococcal infection elicited by immunization with the combination of PspA, PspC, and ClpP","volume":"25","author":"Cao","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0165","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1128\/CDLI.4.4.415-422.1997","article-title":"Standardization of an opsonophagocytic assay for the measurement of functional antibody activity against Streptococcus pneumoniae using differentiated HL-60 cells","volume":"4","author":"Romero-Steiner","year":"1997","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0170","doi-asserted-by":"crossref","first-page":"38609","DOI":"10.1074\/jbc.M112.391060","article-title":"Mycobacterium paratuberculosis CobT activates dendritic cells via engagement of Toll-like receptor 4 resulting in Th1 cell expansion","volume":"287","author":"Byun","year":"2012","journal-title":"J Bio Chem"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0175","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1128\/CVI.00400-07","article-title":"Optimized immune response elicited by a DNA vaccine expressing pneumococcal surface protein A is characterized by a balanced immunoglobulin G1 (IgG1)\/IgG2a Ratio and proinflammatory cytokine production","volume":"15","author":"Ferreira","year":"2008","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0180","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1093\/dnares\/dsp007","article-title":"Transcriptional regulation of the capsular polysaccharide biosynthesis locus of Streptococcus pneumoniae: a bioinformatic analysis","volume":"16","author":"Moscoso","year":"2009","journal-title":"DNA Res"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0185","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1016\/s1016-8478(23)12905-0","article-title":"Comparative analysis of growth-phase-dependent gene expression in virulent and avirulent Streptococcus pneumoniae using a high-density DNA microarray","volume":"21","author":"Ko","year":"2006","journal-title":"Mol Cells"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0190","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1073\/pnas.59.1.86","article-title":"Biological consequences of the replacement of choline by ethanolamine in the cell wall of Pneumococcus: chanin formation, loss of transformability, and loss of autolysis","volume":"59","author":"Tomasz","year":"1968","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0195","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3109\/1040841X.2013.813902","article-title":"Current status and perspectives on protein-based pneumococcal vaccines","volume":"29","author":"Darrieux","year":"2013","journal-title":"Crit Rev Microbiol"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0200","doi-asserted-by":"crossref","first-page":"4870","DOI":"10.1128\/IAI.69.8.4870-4873.2001","article-title":"Intranasal immunization with killed unencapsulated whole cells prevents colonization and invasive disease by capsulated pneumococci","volume":"69","author":"Malley","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0205","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1006\/mpat.1997.0150","article-title":"The hemolytic and complement-activating properties of pneumolysin do not contribute individually to virulence in a pneumococcal bacteremia model","volume":"23","author":"Benton","year":"1997","journal-title":"Microb Pathog"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0210","doi-asserted-by":"crossref","first-page":"e1001191","DOI":"10.1371\/journal.ppat.1001191","article-title":"Pneumolysin activates the NLRP3 inflammasome and promotes proinflammatory cytokines independently of TLR4","volume":"6","author":"McNeela","year":"2010","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0215","doi-asserted-by":"crossref","first-page":"3231","DOI":"10.1016\/j.vaccine.2010.02.014","article-title":"Novel mucosal vaccines generated by genetic conjugation of heterologous proteins to pneumolysin (PLY) from Streptococcus pneumoniae","volume":"28","author":"Douce","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0220","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1038\/sj.embor.7400248","article-title":"Toll-like receptor 2-dependent bacterial sensing does not occur via peptidoglycan recognition","volume":"5","author":"Travassos","year":"2004","journal-title":"EMBO Rep"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0225","doi-asserted-by":"crossref","first-page":"15654","DOI":"10.1074\/jbc.M112.446963","article-title":"Structural reevaluation of Streptococcus pneumoniae Lipoteichoic acid and new insights into its immunostimulatory potency","volume":"288","author":"Gisch","year":"2013","journal-title":"J Biol Chem"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0230","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nri2711","article-title":"The regulation of IL-10 production by immune cells","volume":"10","author":"Saraiva","year":"2010","journal-title":"Nat Rev Immunol"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0235","doi-asserted-by":"crossref","first-page":"e1000159","DOI":"10.1371\/journal.ppat.1000159","article-title":"Interleukin-17A mediates acquired immunity to pneumococcal colonization","volume":"4","author":"Lu","year":"2008","journal-title":"PLoS Pathog"},{"issue":"Suppl. 1","key":"10.1016\/j.vaccine.2014.05.019_bib0240","doi-asserted-by":"crossref","first-page":"S87","DOI":"10.1016\/S0264-410X(00)00285-1","article-title":"The potential for using protein vaccines to protect against otitis media caused by Streptococcus pneumoniae","volume":"19","author":"Briles","year":"2000","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0245","first-page":"99","article-title":"Unsafe injections","author":"Kane","year":"1998","journal-title":"Bull World Health Organization;"},{"key":"10.1016\/j.vaccine.2014.05.019_bib0250","doi-asserted-by":"crossref","first-page":"3131","DOI":"10.1128\/IAI.01696-07","article-title":"The Streptococcus pneumoniae competence regulatory system influences respiratory tract colonization","volume":"76","author":"Kowalko","year":"2008","journal-title":"Infect Immun"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X14006756?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X14006756?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T07:36:55Z","timestamp":1716795415000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X14006756"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7]]},"references-count":50,"journal-issue":{"issue":"33","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["S0264410X14006756"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2014.05.019","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2014,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Mucosal and systemic immunization with a novel attenuated pneumococcal vaccine candidate confer serotype independent protection against Streptococcus pneumoniae in mice","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2014.05.019","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}