{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T06:20:12Z","timestamp":1789885212044,"version":"4.0.1"},"reference-count":40,"publisher":"Elsevier BV","issue":"43","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2014,9]]},"DOI":"10.1016\/j.vaccine.2014.07.092","type":"journal-article","created":{"date-parts":[[2014,8,12]],"date-time":"2014-08-12T19:46:43Z","timestamp":1407872803000},"page":"5570-5576","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":17,"title":["Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae"],"prefix":"10.1016","volume":"32","author":[{"given":"Corinne","family":"Pion","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Courtois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Husson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Clotilde","family":"Bernard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Claire","family":"Nicolai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Talaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuelle","family":"Trannoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Moste","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9gis","family":"Sodoyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Legastelois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1","key":"10.1016\/j.vaccine.2014.07.092_bib0005","doi-asserted-by":"publisher","first-page":"89","DOI":"10.4161\/hv.8.1.19066","article-title":"The importance of influenza prevention for public health","volume":"8","author":"Carrillo-Santisteve","year":"2012","journal-title":"Hum Vaccines Immunother"},{"key":"10.1016\/j.vaccine.2014.07.092_bib0010","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.virusres.2011.09.024","article-title":"Prospects for controlling future pandemics of influenza","volume":"162","author":"Robertson","year":"2011","journal-title":"Virus Res"},{"issue":"1","key":"10.1016\/j.vaccine.2014.07.092_bib0015","doi-asserted-by":"publisher","first-page":"45","DOI":"10.4161\/hv.8.1.18859","article-title":"New technologies for influenza vaccines","volume":"8","author":"Dormitzer","year":"2012","journal-title":"Hum Vaccines Immunother"},{"key":"10.1016\/j.vaccine.2014.07.092_bib0020","series-title":"Influenza vaccines for the future","first-page":"293","article-title":"Cell culture derived influenza vaccines","author":"Dormitzer","year":"2010"},{"issue":"1\u20132","key":"10.1016\/j.vaccine.2014.07.092_bib0025","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.virusres.2011.09.037","article-title":"Novel vaccines against influenza viruses","volume":"162","author":"Kang","year":"2011","journal-title":"Virus Res"},{"key":"10.1016\/j.vaccine.2014.07.092_bib0030","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.2119\/molmed.2012.00147","article-title":"Novel viral vectored vaccines for the prevention of influenza","volume":"18","author":"Lambe","year":"2012","journal-title":"Mol Med"},{"key":"10.1016\/j.vaccine.2014.07.092_bib0035","doi-asserted-by":"publisher","first-page":"737","DOI":"10.1146\/annurev.iy.08.040190.003513","article-title":"Structural basis of immune recognition of influenza virus hemagglutinin","volume":"8","author":"Wilson","year":"1990","journal-title":"Annu Rev Immunol"},{"issue":"5","key":"10.1016\/j.vaccine.2014.07.092_bib0040","doi-asserted-by":"publisher","first-page":"2814","DOI":"10.1128\/JVI.79.5.2814-2822.2005","article-title":"Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls","volume":"79","author":"Fouchier","year":"2005","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2014.07.092_bib0045","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1146\/annurev.biochem.69.1.531","article-title":"Receptor binding and membrane fusion in virus entry: the influenza hemagglutinin","volume":"69","author":"Skehel","year":"2000","journal-title":"Annu Rev Biochem"},{"issue":"2 Pt 1","key":"10.1016\/j.vaccine.2014.07.092_bib0050","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/0092-8674(82)90135-0","article-title":"The antigenic structure of the influenza virus A\/PR\/8\/34 hemagglutinin (H1 subtype)","volume":"31","author":"Caton","year":"1982","journal-title":"Cell"},{"issue":"5796","key":"10.1016\/j.vaccine.2014.07.092_bib0055","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1038\/289373a0","article-title":"Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation","volume":"289","author":"Wiley","year":"1981","journal-title":"Nature"},{"issue":"12","key":"10.1016\/j.vaccine.2014.07.092_bib0060","doi-asserted-by":"publisher","first-page":"2176","DOI":"10.1016\/j.vaccine.2005.11.005","article-title":"Expression and purification of an influenza hemagglutinin \u2013 one step closer to a recombinant protein-based influenza vaccine","volume":"24","author":"Wang","year":"2006","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2014.07.092_bib0065","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1186\/1475-2859-9-29","article-title":"A highly efficient pipeline for protein expression in Leishmania tarentolae using infrared fluorescence protein as marker","volume":"9","author":"Dortay","year":"2010","journal-title":"Microb Cell Fact"},{"issue":"2","key":"10.1016\/j.vaccine.2014.07.092_bib0070","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/S1046-5928(02)00001-3","article-title":"Non-pathogenic trypanosomatid protozoa as a platform for protein research and production","volume":"25","author":"Breitling","year":"2002","journal-title":"Protein Expr Purif"},{"issue":"1","key":"10.1016\/j.vaccine.2014.07.092_bib0075","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.pep.2010.07.011","article-title":"Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae","volume":"75","author":"Ben-Abdallah","year":"2011","journal-title":"Protein Expr Purif"},{"issue":"8","key":"10.1016\/j.vaccine.2014.07.092_bib0080","first-page":"1073","article-title":"High-level expression, purification and characterization of codon-optimized recombinant hemagglutinin 5 proteins in mammalian cells","volume":"123","author":"Yang","year":"2010","journal-title":"Chin Med J (Engl)"},{"issue":"5","key":"10.1016\/j.vaccine.2014.07.092_bib0085","doi-asserted-by":"publisher","first-page":"e10645","DOI":"10.1371\/journal.pone.0010645","article-title":"A single immunization with soluble recombinant trimeric hemagglutinin protects chickens against highly pathogenic avian influenza virus H5N1","volume":"5","author":"Cornelissen","year":"2010","journal-title":"PLoS ONE"},{"issue":"7","key":"10.1016\/j.vaccine.2014.07.092_bib0090","doi-asserted-by":"publisher","first-page":"e11694","DOI":"10.1371\/journal.pone.0011694","article-title":"An influenza A\/H1N1\/2009 hemagglutinin vaccine produced in Escherichia coli","volume":"5","author":"Aguilar-Y\u00e1\u00f1ez","year":"2010","journal-title":"PLoS ONE"},{"issue":"1","key":"10.1016\/j.vaccine.2014.07.092_bib0095","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1007\/s12539-011-0055-z","article-title":"Codon optimization of the major antigen encoding genes of diverse strains of influenza A virus","volume":"3","author":"Mani","year":"2011","journal-title":"Interdiscip Sci"},{"issue":"21","key":"10.1016\/j.vaccine.2014.07.092_bib0100","doi-asserted-by":"publisher","first-page":"11735","DOI":"10.1128\/JVI.01084-12","article-title":"Glycan-dependent immunogenicity of recombinant soluble trimeric hemagglutinin","volume":"86","author":"De Vries","year":"2012","journal-title":"J Virol"},{"issue":"2","key":"10.1016\/j.vaccine.2014.07.092_bib0105","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.molbiopara.2010.12.002","article-title":"Artificial linear episome-based protein expression system for protozoon Leishmania tarentolae","volume":"176","author":"Kushnir","year":"2011","journal-title":"Mol Biochem Parasitol"},{"issue":"3","key":"10.1016\/j.vaccine.2014.07.092_bib0110","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1002\/j.1460-2075.1990.tb08155.x","article-title":"Intracellular transport of soluble and membrane-bound glycoproteins: folding, assembly and secretion of anchor-free influenza hemagglutinin","volume":"9","author":"Singh","year":"1990","journal-title":"EMBO J"},{"issue":"3","key":"10.1016\/j.vaccine.2014.07.092_bib0115","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1128\/JVI.60.3.833-839.1986","article-title":"Quaternary structure of influenza virus hemagglutinin after acid treatment","volume":"60","author":"Doms","year":"1986","journal-title":"J Virol"},{"issue":"5","key":"10.1016\/j.vaccine.2014.07.092_bib0120","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1086\/590913","article-title":"An adjuvanted, low-dose, pandemic influenza A (H5N1) vaccine candidate is safe, immunogenic, and induces cross-reactive immune responses in healthy adults","volume":"198","author":"Levie","year":"2008","journal-title":"J Infect Dis"},{"issue":"18","key":"10.1016\/j.vaccine.2014.07.092_bib0125","doi-asserted-by":"publisher","first-page":"3076","DOI":"10.1016\/j.vaccine.2010.02.050","article-title":"AF03-adjuvanted and non-adjuvanted pandemic influenza A (H1N1) 2009 vaccines induce strong antibody responses in seasonal influenza vaccine-primed and unprimed mice","volume":"28","author":"Caillet","year":"2010","journal-title":"Vaccine"},{"issue":"9523","key":"10.1016\/j.vaccine.2014.07.092_bib0130","doi-asserted-by":"publisher","first-page":"1657","DOI":"10.1016\/S0140-6736(06)68656-X","article-title":"Safety and immunogenicity of an inactivated split-virion influenza A\/Vietnam\/1194\/2004(H5N1) vaccine: phase I randomised trial","volume":"367","author":"Bresson","year":"2006","journal-title":"Lancet"},{"issue":"7","key":"10.1016\/j.vaccine.2014.07.092_bib0140","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1586\/14760584.2013.811188","article-title":"An update on safety and immunogenicity of vaccines containing emulsion-based adjuvants","volume":"12","author":"Fox","year":"2013","journal-title":"Expert Rev Vaccines"},{"issue":"29","key":"10.1016\/j.vaccine.2014.07.092_bib0145","doi-asserted-by":"publisher","first-page":"4341","DOI":"10.1016\/j.vaccine.2011.09.061","article-title":"The mechanism of action of MF59 \u2013 an innately attractive adjuvant formulation","volume":"30","author":"O\u2019Hagan","year":"2012","journal-title":"Vaccine"},{"issue":"3","key":"10.1016\/j.vaccine.2014.07.092_bib0150","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.biologicals.2009.02.019","article-title":"A novel vaccine adjuvant for recombinant flu antigens","volume":"37","author":"Chang","year":"2009","journal-title":"Biologicals"},{"key":"10.1016\/j.vaccine.2014.07.092_bib0155","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1186\/1743-422X-8-524","article-title":"Yeast expressed recombinant hemagglutinin protein of novel H1N1 elicits neutralising antibodies in rabbits and mice","volume":"8","author":"Athmaram","year":"2011","journal-title":"Virol J"},{"issue":"1","key":"10.1016\/j.vaccine.2014.07.092_bib0160","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.jviromet.2012.07.026","article-title":"Recombinant haemagglutinin protein of highly pathogenic avian influenza A (H5N1) virus expressed in Pichia pastoris elicits a neutralizing antibody response in mice","volume":"187","author":"Murugan","year":"2013","journal-title":"J Virol Methods"},{"issue":"52","key":"10.1016\/j.vaccine.2014.07.092_bib0165","doi-asserted-by":"publisher","first-page":"7498","DOI":"10.1016\/j.vaccine.2012.10.054","article-title":"Insect cell-expressed hemagglutinin with CpG oligodeoxynucleotides plus alum as an adjuvant is a potential pandemic influenza vaccine candidate","volume":"30","author":"Gong","year":"2012","journal-title":"Vaccine"},{"issue":"1\u20132","key":"10.1016\/j.vaccine.2014.07.092_bib0170","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.virusres.2004.02.025","article-title":"Immunogenicity and protective efficacy of influenza vaccination","volume":"103","author":"Hannoun","year":"2004","journal-title":"Virus Res"},{"issue":"44","key":"10.1016\/j.vaccine.2014.07.092_bib0175","doi-asserted-by":"publisher","first-page":"6234","DOI":"10.1016\/j.vaccine.2009.07.107","article-title":"Preparation, characterization, and immunogenicity in mice of a recombinant influenza H5 hemagglutinin vaccine against the avian H5N1 A\/Vietnam\/1203\/2004 influenza virus","volume":"27","author":"Biesova","year":"2009","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.vaccine.2014.07.092_bib0180","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.virol.2010.09.009","article-title":"Classical swine H1N1 influenza viruses confer cross protection from swine-origin 2009 pandemic H1N1 influenza virus infection in mice and ferrets","volume":"408","author":"Min","year":"2010","journal-title":"Virology"},{"issue":"1","key":"10.1016\/j.vaccine.2014.07.092_bib0185","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1586\/erv.09.148","article-title":"Animal models for the preclinical evaluation of candidate influenza vaccines","author":"Bodewes","year":"2010","journal-title":"Expert Rev Vaccines"},{"issue":"7","key":"10.1016\/j.vaccine.2014.07.092_bib0190","doi-asserted-by":"publisher","first-page":"e11548","DOI":"10.1371\/journal.pone.0011548","article-title":"Properly folded bacterially expressed H1N1 hemagglutinin globular head and ectodomain vaccines protect ferrets against H1N1 pandemic influenza virus","volume":"5","author":"Khurana","year":"2010","journal-title":"PLoS ONE"},{"issue":"12","key":"10.1016\/j.vaccine.2014.07.092_bib0195","doi-asserted-by":"publisher","first-page":"1357","DOI":"10.1007\/s40265-013-0103-6","article-title":"Recombinant trivalent influenza vaccine (Flublok\u00ae): a review of its use in then prevention of seasonal influenza in adults","author":"Yang","year":"2013","journal-title":"Drugs"},{"issue":"1","key":"10.1016\/j.vaccine.2014.07.092_bib0200","doi-asserted-by":"publisher","first-page":"45","DOI":"10.4161\/hv.8.1.18859","article-title":"New technologies for influenza vaccines","author":"Dormitzer","year":"2012","journal-title":"Hum Vaccines Immunother"},{"issue":"12","key":"10.1016\/j.vaccine.2014.07.092_bib0205","doi-asserted-by":"publisher","first-page":"e50830","DOI":"10.1371\/journal.pone.0050830","article-title":"Evaluations for in vitro correlates of immunogenicity of inactivated influenza a H5, H7 and H9 vaccines in humans","volume":"7","author":"Couch","year":"2012","journal-title":"PLoS ONE"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X14010743?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X14010743?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,8,23]],"date-time":"2020-08-23T04:55:50Z","timestamp":1598158550000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X14010743"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":40,"journal-issue":{"issue":"43","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["S0264410X14010743"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2014.07.092","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2014,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Characterization and immunogenicity in mice of recombinant influenza haemagglutinins produced in Leishmania tarentolae","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2014.07.092","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}