{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:15:57Z","timestamp":1784888157627,"version":"3.55.0"},"reference-count":36,"publisher":"Elsevier BV","issue":"43","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2014,9]]},"DOI":"10.1016\/j.vaccine.2014.08.010","type":"journal-article","created":{"date-parts":[[2014,8,17]],"date-time":"2014-08-17T13:30:24Z","timestamp":1408282224000},"page":"5650-5656","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":22,"title":["Evaluation and validation of a serum bactericidal antibody assay for Haemophilus influenzae type b and the threshold of protection"],"prefix":"10.1016","volume":"32","author":[{"given":"Kelly","family":"Townsend","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0856-2476","authenticated-orcid":false,"given":"Shamez N.","family":"Ladhani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Findlow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ray","family":"Borrow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2014.08.010_bib0005","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1084\/jem.53.4.471","article-title":"Variation and type specificity in the bacterial species Haemophilus influenzae","volume":"53","author":"Pittman","year":"1931","journal-title":"J Exp Med"},{"issue":"April (2)","key":"10.1016\/j.vaccine.2014.08.010_bib0010","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1128\/CMR.13.2.302-317.2000","article-title":"Worldwide Haemophilus influenzae type b disease at the beginning of the 21st century: global analysis of the disease burden 25 years after the use of the polysaccharide vaccine and a decade after the advent of conjugates","volume":"13","author":"Peltola","year":"2000","journal-title":"Clin Microbiol Rev"},{"issue":"April (4)","key":"10.1016\/j.vaccine.2014.08.010_bib0015","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/0882-4010(91)90010-8","article-title":"Complement-mediated serum activities against genetically defined capsular transformants of Haemophilus influenzae","volume":"10","author":"Swift","year":"1991","journal-title":"Microb Pathog"},{"issue":"February (2)","key":"10.1016\/j.vaccine.2014.08.010_bib0020","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1016\/j.clinthera.2011.11.027","article-title":"Two decades of experience with the Haemophilus influenzae serotype b conjugate vaccine in the United Kingdom","volume":"34","author":"Ladhani","year":"2012","journal-title":"Clin Ther"},{"issue":"March (3)","key":"10.1016\/j.vaccine.2014.08.010_bib0025","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1203\/00006450-197303000-00001","article-title":"Quantitative measurement of \u201cnatural\u201d and immunization-induced Haemophilus influenzae type b capsular polysaccharide antibodies","volume":"7","author":"Robbins","year":"1973","journal-title":"Pediatr Res"},{"issue":"November (5)","key":"10.1016\/j.vaccine.2014.08.010_bib0030","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1542\/peds.60.5.730","article-title":"Haemophilus influenzae type b capsular polysaccharide vaccine in children: a double-blind field study of 100,000 vaccinees 3 months to 5 years of age in Finland","volume":"60","author":"Peltola","year":"1977","journal-title":"Pediatrics"},{"issue":"June (6)","key":"10.1016\/j.vaccine.2014.08.010_bib0035","doi-asserted-by":"publisher","first-page":"1100","DOI":"10.1093\/infdis\/147.6.1100","article-title":"The protective level of serum antibodies to the capsular polysaccharide of Haemophilus influenzae type b","volume":"147","author":"Kayhty","year":"1983","journal-title":"J Infect Dis"},{"issue":"October (15)","key":"10.1016\/j.vaccine.2014.08.010_bib0040","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1056\/NEJM198710083171503","article-title":"Prevention of Haemophilus influenzae type b infections in high-risk infants treated with bacterial polysaccharide immune globulin","volume":"317","author":"Santosham","year":"1987","journal-title":"N Engl J Med"},{"issue":"December (1\u20132)","key":"10.1016\/j.vaccine.2014.08.010_bib0045","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/0022-1759(90)90264-V","article-title":"An ELISA employing a Haemophilus influenzae type b oligosaccharide\u2013human serum albumin conjugate correlates with the radioantigen binding assay","volume":"135","author":"Phipps","year":"1990","journal-title":"J Immunol Methods"},{"issue":"April (4)","key":"10.1016\/j.vaccine.2014.08.010_bib0050","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1086\/517407","article-title":"Antibody avidity as a surrogate marker of successful priming by Haemophilus influenzae type b conjugate vaccines following infant immunization","volume":"177","author":"Goldblatt","year":"1998","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2014.08.010_bib0055","doi-asserted-by":"crossref","first-page":"273","DOI":"10.4049\/jimmunol.24.4.273","article-title":"Influenzal meningitis: the relation of age incidence to the bactericidal power of blood against the causal organism","volume":"24","author":"Fothergill","year":"1933","journal-title":"J Immunol"},{"issue":"10","key":"10.1016\/j.vaccine.2014.08.010_bib0060","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1016\/0264-410X(93)90124-G","article-title":"Characterization of the serum antibody response induced by Haemophilus influenzae type b tetanus protein-conjugate vaccine in infants receiving a DTP-combined vaccine from 2 months of age","volume":"11","author":"Barra","year":"1993","journal-title":"Vaccine"},{"issue":"April (4 Pt 1)","key":"10.1016\/j.vaccine.2014.08.010_bib0065","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1203\/00006450-199004000-00008","article-title":"Variability in the functional activity of vaccine-induced antibody to Haemophilus influenzae type b","volume":"27","author":"Amir","year":"1990","journal-title":"Pediatr Res"},{"issue":"January (1)","key":"10.1016\/j.vaccine.2014.08.010_bib0070","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1093\/infdis\/153.1.8","article-title":"Functional characterization of human IgG, IgM, and IgA antibody directed to the capsule of Haemophilus influenzae type b","volume":"153","author":"Schreiber","year":"1986","journal-title":"J Infect Dis"},{"issue":"July (1)","key":"10.1016\/j.vaccine.2014.08.010_bib0075","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1093\/infdis\/162.1.163","article-title":"Bactericidal and opsonic activity of IgG1 and IgG2 anticapsular antibodies to Haemophilus influenzae type b","volume":"162","author":"Amir","year":"1990","journal-title":"J Infect Dis"},{"issue":"March (11)","key":"10.1016\/j.vaccine.2014.08.010_bib0080","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1001\/jama.1992.03480110065035","article-title":"Avidity and bactericidal activity of antibody elicited by different Haemophilus influenzae type b conjugate vaccines. The Vaccine Study Group","volume":"267","author":"Schlesinger","year":"1992","journal-title":"JAMA"},{"issue":"November (6)","key":"10.1016\/j.vaccine.2014.08.010_bib0085","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1128\/CDLI.8.6.1115-1119.2001","article-title":"Functional antibody activity elicited by fractional doses of Haemophilus influenzae type b conjugate vaccine (polyribosylribitol phosphate-tetanus toxoid conjugate)","volume":"8","author":"Romero-Steiner","year":"2001","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.vaccine.2014.08.010_bib0090","series-title":"Guidance for industry: bioanalytical method validation","author":"Food and Drug Administration (FDA)","year":"2001"},{"issue":"March (2)","key":"10.1016\/j.vaccine.2014.08.010_bib0095","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1128\/CDLI.4.2.156-167.1997","article-title":"Standardization and a multilaboratory comparison of Neisseria meningitidis serogroup A and C serum bactericidal assays. The Multilaboratory Study Group","volume":"4","author":"Maslanka","year":"1997","journal-title":"Clin Diagn Lab Immunol"},{"issue":"February (2)","key":"10.1016\/j.vaccine.2014.08.010_bib0100","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1128\/CVI.00420-08","article-title":"Immunogenicity of a reduced schedule of meningococcal group C conjugate vaccine given concomitantly with the Prevenar and Pediacel vaccines in healthy infants in the United Kingdom","volume":"16","author":"Southern","year":"2009","journal-title":"Clin Vaccine Immunol"},{"issue":"January (1)","key":"10.1016\/j.vaccine.2014.08.010_bib0105","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1128\/CVI.00384-09","article-title":"Kinetics of antibody persistence following administration of a combination meningococcal serogroup C and haemophilus influenzae type b conjugate vaccine in healthy infants in the United Kingdom primed with a monovalent meningococcal serogroup C vaccine","volume":"17","author":"Borrow","year":"2010","journal-title":"Clin Vaccine Immunol"},{"issue":"July (4)","key":"10.1016\/j.vaccine.2014.08.010_bib0110","first-page":"872","article-title":"Comparison of a multiplex flow cytometric assay with enzyme-linked immunosorbent assay for auantitation of antibodies to tetanus, diphtheria, and Haemophilus influenzae Type b","volume":"9","author":"Pickering","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"issue":"February (2)","key":"10.1016\/j.vaccine.2014.08.010_bib0115","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1128\/IAI.13.2.581-589.1976","article-title":"Synthesis and release of polyribophosphate by Haemophilus influenzae type b in vitro","volume":"13","author":"Anderson","year":"1976","journal-title":"Infect Immun"},{"issue":"January (1)","key":"10.1016\/j.vaccine.2014.08.010_bib0120","first-page":"109","article-title":"Standardization of Neisseria meningitidis serogroup B colorimetric serum bactericidal assay","volume":"9","author":"Rodriguez","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"10.1016\/j.vaccine.2014.08.010_bib0125","series-title":"Requirements for meningococcal polysaccharide vaccine (requirements for biological substances no. 23). W.H.O. Tech. Rep. Ser. ed.","author":"World Health Organisation","year":"1976"},{"issue":"October (10)","key":"10.1016\/j.vaccine.2014.08.010_bib0130","doi-asserted-by":"publisher","first-page":"1362","DOI":"10.1128\/CVI.00154-07","article-title":"Quality of the Haemophilus influenzae type b (Hib) antibody response induced by diphtheria\u2013tetanus\u2013acellular pertussis\/Hib combination vaccines","volume":"14","author":"Denoel","year":"2007","journal-title":"Clin Vaccine Immunol"},{"issue":"January (1)","key":"10.1016\/j.vaccine.2014.08.010_bib0135","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/S0732-8893(01)00338-8","article-title":"Influence of CO(2) incubation on quinolone activity against Streptococcus pneumoniae and Haemophilus influenzae","volume":"42","author":"Bolmstrom","year":"2002","journal-title":"Diagn Microbiol Infect Dis"},{"issue":"September (1\u20132)","key":"10.1016\/j.vaccine.2014.08.010_bib0140","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/j.jim.2004.06.021","article-title":"High-throughput imaging of bacterial colonies grown on filter plates with application to serum bactericidal assays","volume":"292","author":"Liu","year":"2004","journal-title":"J Immunol Methods"},{"issue":"March (17\u201318)","key":"10.1016\/j.vaccine.2014.08.010_bib0145","doi-asserted-by":"publisher","first-page":"2218","DOI":"10.1016\/j.vaccine.2005.01.070","article-title":"Validation of the serum bactericidal assay for measurement of functional antibodies against group B meningococci associated with vaccine trials","volume":"23","author":"Martin","year":"2005","journal-title":"Vaccine"},{"issue":"December (6)","key":"10.1016\/j.vaccine.2014.08.010_bib0150","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1586\/14760584.5.6.851","article-title":"Long-term protection in children with meningococcal C conjugate vaccination: lessons learned","volume":"5","author":"Borrow","year":"2006","journal-title":"Expert Rev Vaccines"},{"issue":"9 Suppl. June","key":"10.1016\/j.vaccine.2014.08.010_bib0155","doi-asserted-by":"publisher","first-page":"S30","DOI":"10.1016\/0264-410X(91)90178-9","article-title":"Comparative immunogenicity of Haemophilus influenzae type b polysaccharide\u2013protein conjugate vaccines","author":"Granoff","year":"1991","journal-title":"Vaccine"},{"issue":"July (7)","key":"10.1016\/j.vaccine.2014.08.010_bib0160","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1128\/CVI.00068-09","article-title":"Investigation of different group A immunoassays following one dose of meningococcal group A conjugate vaccine or A\/C polysaccharide vaccine in adults","volume":"16","author":"Findlow","year":"2009","journal-title":"Clin Vaccine Immunol"},{"issue":"January (1)","key":"10.1016\/j.vaccine.2014.08.010_bib0165","doi-asserted-by":"publisher","first-page":"102","DOI":"10.4161\/hv.7.1.13351","article-title":"Haemophilus influenzae vaccine candidate outer membrane protein P6 is not conserved in all strains","volume":"7","author":"Chang","year":"2011","journal-title":"Hum Vaccin"},{"issue":"December (12)","key":"10.1016\/j.vaccine.2014.08.010_bib0170","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1128\/CVI.00221-06","article-title":"Antibodies specific for the high-molecular-weight adhesion proteins of nontypeable Haemophilus influenzae are opsonophagocytic for both homologous and heterologous strains","volume":"13","author":"Winter","year":"2006","journal-title":"Clin Vaccine Immunol"},{"issue":"January\u2013February (1)","key":"10.1016\/j.vaccine.2014.08.010_bib0175","doi-asserted-by":"publisher","first-page":"24","DOI":"10.4161\/hv.2.1.2445","article-title":"Immunogenicity and reactogenicity to Haemophilus influenzae type B (Hib) conjugate vaccine among rural Alaska adults","volume":"2","author":"Dentinger","year":"2006","journal-title":"Hum Vaccin."},{"issue":"Aug (4)","key":"10.1016\/j.vaccine.2014.08.010_bib0180","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1017\/S0950268804002122","article-title":"Long-term impact of vaccination on Haemophilus influenzae type b (Hib) carriage in the United Kingdom","volume":"132","author":"McVernon","year":"2004","journal-title":"Epidemiol Infect."}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X14011232?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X14011232?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,7,16]],"date-time":"2023-07-16T02:52:01Z","timestamp":1689475921000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X14011232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":36,"journal-issue":{"issue":"43","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["S0264410X14011232"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2014.08.010","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2014,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Evaluation and validation of a serum bactericidal antibody assay for Haemophilus influenzae type b and the threshold of protection","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2014.08.010","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2014 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}