{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T15:10:57Z","timestamp":1793459457344,"version":"4.5.0"},"reference-count":40,"publisher":"Elsevier BV","issue":"39","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"National Agriculture and Food Research Organization of Japan"},{"name":"R&D","award":["AS2311903E"],"award-info":[{"award-number":["AS2311903E"]}]},{"DOI":"10.13039\/501100003993","name":"Ministry of Agriculture, Forestry, and Fisheries of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003993","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2015,9]]},"DOI":"10.1016\/j.vaccine.2015.07.074","type":"journal-article","created":{"date-parts":[[2015,8,5]],"date-time":"2015-08-05T16:09:40Z","timestamp":1438790980000},"page":"5204-5211","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":30,"title":["Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs"],"prefix":"10.1016","volume":"33","author":[{"given":"Natsumi","family":"Takeyama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Yuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2441-5062","authenticated-orcid":false,"given":"Daisuke","family":"Tokuhara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuki","family":"Oroku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mio","family":"Mejima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiho","family":"Kurokawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2231-2518","authenticated-orcid":false,"given":"Masaharu","family":"Kuroda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiaki","family":"Kodama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinya","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kiyono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2015.07.074_bib0205","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0378-1135(99)00108-X","article-title":"Seroprevalence of F4+ enterotoxigenic Escherichia coli in regions with different pig farm densities","volume":"69","author":"Van den Broeck","year":"1999","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0210","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/978-1-4615-4143-1_7","article-title":"Comparative pathology of bacterial enteric diseases of swine","volume":"473","author":"Moxley","year":"1999","journal-title":"Adv Exp Med Biol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0215","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.vetmic.2007.02.018","article-title":"Prevalence of virulence genes in Escherichia coli strains recently isolated from young pigs with diarrhea in the US","volume":"123","author":"Zhang","year":"2007","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0220","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1177\/104063870601800405","article-title":"Frequency of enteropathogen detection in suckling and weaned pigs with diarrhea in Japan","volume":"18","author":"Katsuda","year":"2006","journal-title":"J Vet Diagn Invest"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0225","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.vetimm.2012.09.024","article-title":"ETEC vaccination in pigs","volume":"152","author":"Melkebeek","year":"2013","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0230","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1128\/MMBR.46.2.129-161.1982","article-title":"Host-specific fimbrial adhesins of noninvasive enterotoxigenic Escherichia coli strains","volume":"46","author":"Gaastra","year":"1982","journal-title":"Microbiol Rev"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0235","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1016\/j.vetmic.2012.09.025","article-title":"Protection of piglets against enteric colibacillosis by intranasal immunization with K88ac (F4ac) fimbriae and heat labile enterotoxin of Escherichia coli","volume":"162","author":"Lin","year":"2013","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0240","first-page":"57","article-title":"The transfer of immunoglobins IgG, IgA and IgM from serum to colostrum and milk in the sow","volume":"24","author":"Bourne","year":"1973","journal-title":"Immunology"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0245","doi-asserted-by":"crossref","first-page":"43","DOI":"10.20506\/rst.17.1.1096","article-title":"Immunoglobulin diversity, B-cell and antibody repertoire development in large farm animals","volume":"17","author":"Butler","year":"1998","journal-title":"Rev Sci Tech"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0250","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1016\/j.dci.2008.07.007","article-title":"Humoral and cellular factors of maternal immunity in swine","volume":"33","author":"Salmon","year":"2008","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0255","first-page":"259","article-title":"Enterotoxigenic Escherichia coli (ETEC) in farm animals","volume":"30","author":"Nagy","year":"1999","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0260","doi-asserted-by":"crossref","first-page":"2797","DOI":"10.1016\/j.micinf.2006.08.011","article-title":"In vivo expression of the heat stable (estA) and heat labile (eltB) toxin genes of enterotoxigenic Escherichia coli (ETEC)","volume":"8","author":"Sj\u00f6ling","year":"2006","journal-title":"Microbes Infect"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0265","doi-asserted-by":"crossref","first-page":"2132","DOI":"10.3390\/toxins2082132","article-title":"Toxin mediated diarrhea in the 21st century: the pathophysiology of intestinal ion transport in the course of ETEC, V. cholerae and rotavirus infection","volume":"2","author":"Kopic","year":"2010","journal-title":"Toxins"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0270","doi-asserted-by":"crossref","first-page":"3141","DOI":"10.1128\/IAI.01743-07","article-title":"Comparison of the contributions of heat-labile enterotoxin and heat-stable enterotoxin b to the virulence of enterotoxigenic Escherichia coli in F4ac receptor-positive young pigs","volume":"76","author":"Erume","year":"2008","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0275","doi-asserted-by":"crossref","first-page":"1458","DOI":"10.1016\/j.vaccine.2013.01.030","article-title":"Oral immunization of a live attenuated Escherichia coli strain expressing a holotoxin-structured adhesin-toxoid fusion (1FaeG-FedF-LTA2:5LTB) protected young pigs against enterotoxigenic E. coli (ETEC) infection","volume":"31","author":"Ruan","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0280","doi-asserted-by":"crossref","first-page":"10986","DOI":"10.1073\/pnas.0703766104","article-title":"Rice-based mucosal vaccine as a global strategy for cold-chain- and needle-free vaccination","volume":"104","author":"Nochi","year":"2007","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0285","doi-asserted-by":"crossref","first-page":"6538","DOI":"10.4049\/jimmunol.0901480","article-title":"A rice-based oral cholera vaccine induces macaque-specific systemic neutralizing antibodies but does not influence pre-existing intestinal immunity","volume":"183","author":"Nochi","year":"2009","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0290","doi-asserted-by":"crossref","first-page":"8794","DOI":"10.1073\/pnas.0914121107","article-title":"Secretory IgA-mediated protection against V. cholerae and heat-labile enterotoxin-producing enterotoxigenic Escherichia coli by rice-based vaccine","volume":"107","author":"Tokuhara","year":"2010","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0295","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1128\/IAI.42.2.683-691.1983","article-title":"Immunological relationships between cholera toxin and Escherichia coli heat-labile enterotoxin","volume":"42","author":"Gilligan","year":"1983","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0300","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1093\/infdis\/158.2.372","article-title":"Cross-protection by B subunit-whole cell cholera vaccine against diarrhea associated with heat-labile toxin-producing enterotoxigenic Escherichia coli: results of a large-scale field trial","volume":"158","author":"Clemens","year":"1988","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0305","doi-asserted-by":"crossref","first-page":"1285","DOI":"10.1016\/0140-6736(91)92590-X","article-title":"Prevention of travellers\u2019 diarrhoea by oral B-subunit\/whole-cell cholera vaccine","volume":"338","author":"Peltola","year":"1991","journal-title":"Lancet"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0310","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1016\/S0140-6736(03)13971-2","article-title":"Protection from natural infections with enterotoxigenic Escherichia coli: longitudinal study","volume":"362","author":"Steinsland","year":"2003","journal-title":"Lancet"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0315","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1111\/pbi.12071","article-title":"Induction of toxin-specific neutralizing immunity by molecularly uniform rice-based oral cholera toxin B subunit vaccine without plant-associated sugar modification","volume":"11","author":"Yuki","year":"2013","journal-title":"Plant Biotechnol J"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0320","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/BF02122706","article-title":"Evaluation of a defined medium for the production of both thermolabile (LT) and thermostable (ST) enterotoxins of Escherichia coli","volume":"171","author":"Fontes","year":"1982","journal-title":"Med Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0325","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1038\/nbt0398-292","article-title":"Efficacy of a food plant-based oral cholera toxin B subunit vaccine","volume":"16","author":"Arakawa","year":"1998","journal-title":"Nat Biotechnol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0330","doi-asserted-by":"crossref","first-page":"3374","DOI":"10.1016\/S0264-410X(03)00336-0","article-title":"Transfer of antibody via mother's milk","volume":"21","author":"Van de Perre","year":"2003","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0335","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.vetimm.2012.02.002","article-title":"Development of a novel live vaccine delivering enterotoxigenic Escherichia coli fimbrial antigens to prevent post-weaning diarrhea in piglets","volume":"146","author":"Hur","year":"2012","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0340","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/0264-410X(95)00192-4","article-title":"Parenteral vaccination of mice and piglets with F4+ Escherichia coli suppresses the enteric anti-F4 response upon oral infection","volume":"14","author":"Bianchi","year":"1996","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0345","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1128\/JVI.72.1.330-338.1998","article-title":"Antibody-secreting cell responses and protective immunity assessed in gnotobiotic pigs inoculated orally or intramuscularly with inactivated human rotavirus","volume":"72","author":"Yuan","year":"1998","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0350","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0378-1135(99)00166-2","article-title":"Active oral immunization of suckling piglets to prevent colonization after weaning by enterotoxigenic Escherichia coli with fimbriae F18","volume":"71","author":"Bertschingera","year":"2000","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0355","doi-asserted-by":"crossref","first-page":"4782","DOI":"10.1128\/AEM.02885-05","article-title":"Comparison of virulence gene profiles of Escherichia coli strains isolated from healthy and diarrheic swine","volume":"72","author":"Chapman","year":"2006","journal-title":"Appl Environ Microbiol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0360","doi-asserted-by":"crossref","first-page":"2020","DOI":"10.1016\/S0264-410X(98)00406-X","article-title":"Induction of immune responses in pigs following oral administration of purified F4 fimbriae","volume":"17","author":"Van den Broeck","year":"1999","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0365","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1128\/IAI.67.2.520-526.1999","article-title":"Receptor-dependent immune responses in pigs after oral immunization with F4 fimbriae","volume":"67","author":"Van den Broeck","year":"1999","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0370","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.vetimm.2007.06.018","article-title":"Mucosal immunization of piglets with purified F18 fimbriae does not protect against F18+ Escherichia coli infection","volume":"120","author":"Verdonck","year":"2007","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0375","first-page":"59","article-title":"Isolation of Escherichia coli from piglets in South Korea with diarrhea and characteristics of the virulence genes","volume":"74","author":"Kim","year":"2010","journal-title":"Can J Vet Res"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0380","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/S0378-1135(01)00504-1","article-title":"Prevalence of serogroups and virulence genes in Escherichia coli associated with postweaning diarrhoea and edema disease in pigs and a comparison of diagnostic approaches","volume":"85","author":"Frydendahl","year":"2002","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0385","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1177\/104063870802000413","article-title":"Development of multiplex polymerase chain reaction assays for detecting enterotoxigenic Escherichia coli and their application to field isolates from piglets with diarrhea","volume":"20","author":"Lee","year":"2008","journal-title":"J Vet Diagn Invest"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0390","doi-asserted-by":"crossref","first-page":"e42405","DOI":"10.1371\/journal.pone.0042405","article-title":"Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco","volume":"7","author":"Kolotilin","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0395","doi-asserted-by":"crossref","first-page":"11809","DOI":"10.1073\/pnas.1301975110","article-title":"Orally fed seeds producing designer IgAs protect weaned piglets against enterotoxigenic Escherichia coli infection","volume":"110","author":"Virdi","year":"2013","journal-title":"Proc Natl Acad Sci U S A"},{"key":"10.1016\/j.vaccine.2015.07.074_bib0400","doi-asserted-by":"crossref","first-page":"3372","DOI":"10.1021\/pr4002146","article-title":"MucoRice-cholera toxin B-subunit, a rice-based oral cholera vaccine, down-regulates the expression of a-amylase\/trypsin inhibitor-like protein family as major rice allergens","volume":"12","author":"Kurokawa","year":"2013","journal-title":"J Proteome Res"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X15010518?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X15010518?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,6]],"date-time":"2020-09-06T22:18:30Z","timestamp":1599430710000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X15010518"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9]]},"references-count":40,"journal-issue":{"issue":"39","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["S0264410X15010518"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2015.07.074","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2015,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2015.07.074","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}