{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:13:52Z","timestamp":1770819232732,"version":"3.50.1"},"reference-count":51,"publisher":"Elsevier BV","issue":"42","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100005014","name":"Avicenna Research Institute","doi-asserted-by":"publisher","award":["88-49"],"award-info":[{"award-number":["88-49"]}],"id":[{"id":"10.13039\/501100005014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004484","name":"Tehran University of Medical Sciences","doi-asserted-by":"publisher","award":["8723"],"award-info":[{"award-number":["8723"]}],"id":[{"id":"10.13039\/501100004484","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2015,10]]},"DOI":"10.1016\/j.vaccine.2015.09.013","type":"journal-article","created":{"date-parts":[[2015,9,16]],"date-time":"2015-09-16T11:46:25Z","timestamp":1442403985000},"page":"5532-5538","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":39,"title":["Simultaneous immunization of mice with Omp31 and TF provides protection against Brucella melitensis infection"],"prefix":"10.1016","volume":"33","author":[{"given":"Amir","family":"Ghasemi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmood","family":"Jeddi-Tehrani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josef","family":"Mautner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Hossein","family":"Salari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir-Hassan","family":"Zarnani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2015.09.013_bib0260","doi-asserted-by":"crossref","first-page":"124509","DOI":"10.1155\/2010\/124509","article-title":"Importance of lipopolysaccharide and cyclic beta-1,2-glucans in Brucella-mammalian infections","volume":"2010","author":"Haag","year":"2010","journal-title":"Int J Microbiol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0265","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1016\/S1286-4579(01)01427-7","article-title":"Classification of Brucella spp. isolated from marine mammals by DNA polymorphism at the Omp2 locus","volume":"3","author":"Cloeckaert","year":"2001","journal-title":"Microbes Infect"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0270","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1006\/mpat.2000.0373","article-title":"Residual virulence of Brucella abortus in the absence of the cytochrome bc(1) complex in a murine model in vitro and in vivo","volume":"29","author":"Ko","year":"2000","journal-title":"Microb Pathog"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0275","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/S0378-1135(97)00118-1","article-title":"The characterisation of Brucella strains isolated from marine mammals","volume":"57","author":"Jahans","year":"1997","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0280","first-page":"233","article-title":"Brucellosis in humans \u2013 etiology, diagnostics, clinical forms","volume":"20","author":"Galinska","year":"2013","journal-title":"Ann Agric Environ Med"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0285","doi-asserted-by":"crossref","first-page":"289","DOI":"10.3325\/cmj.2010.51.289","article-title":"Brucellosis \u2013 regionally emerging zoonotic disease?","volume":"51","author":"Gwida","year":"2010","journal-title":"Croat Med J"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0290","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3389\/fcimb.2012.00002","article-title":"Analyses of Brucella pathogenesis, host immunity, and vaccine targets using systems biology and bioinformatics","volume":"2","author":"He","year":"2012","journal-title":"Front Cell Inf Microbiol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0295","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0300","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/j.vaccine.2013.12.028","article-title":"Evaluation of humoral and cellular immune responses to BP26 and Omp31 epitopes in the attenuated Brucella melitensis vaccinated sheep","volume":"32","author":"Wang","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0305","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/S0378-1135(02)00255-9","article-title":"Brucellosis vaccines: past, present and future","volume":"90","author":"Schurig","year":"2002","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0310","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1016\/S0034-5288(18)30992-5","article-title":"Comparison of subcutaneous and conjunctival routes of Rev 1 vaccination for the prophylaxis of Brucella ovis infection in rams","volume":"48","author":"Marin","year":"1990","journal-title":"Res Vet Sci"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0315","doi-asserted-by":"crossref","first-page":"e29552","DOI":"10.1371\/journal.pone.0029552","article-title":"Immunization of mice with recombinant protein CobB or AsnC confers protection against Brucella abortus infection","volume":"7","author":"Fu","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0320","first-page":"19","article-title":"Immune reactivity of Brucella melitensis-vaccinated rabbit serum with recombinant Omp31 and DnaK proteins","volume":"5","author":"Ghasemi","year":"2013","journal-title":"Iran J Microbiol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0325","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s11515-012-1196-0","article-title":"Progress in vaccine development","volume":"8","author":"Yang","year":"2013","journal-title":"Front Biol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0330","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1016\/0140-6736(93)91571-3","article-title":"Brucella melitensis Rev-1 vaccine as a cause of human brucellosis","volume":"342","author":"Blasco","year":"1993","journal-title":"Lancet"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0335","doi-asserted-by":"crossref","first-page":"a012476","DOI":"10.1101\/cshperspect.a012476","article-title":"Vaccines, reverse vaccinology, and bacterial pathogenesis","volume":"3","author":"Delany","year":"2013","journal-title":"Cold Spring Harbor Perspect Med"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0340","doi-asserted-by":"crossref","first-page":"207","DOI":"10.20506\/rst.32.1.2201","article-title":"Recent developments in livestock and wildlife brucellosis vaccination","volume":"32","author":"Olsen","year":"2013","journal-title":"Rev Sci Tech"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0345","doi-asserted-by":"crossref","first-page":"3740","DOI":"10.4049\/jimmunol.1302561","article-title":"Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis","volume":"192","author":"Vitry","year":"2014","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0350","doi-asserted-by":"crossref","first-page":"303","DOI":"10.4161\/bioe.21005","article-title":"Identification of Brucella abortus virulence proteins that modulate the host immune response","volume":"3","author":"Wang","year":"2012","journal-title":"Bioengineered"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0355","doi-asserted-by":"crossref","first-page":"7297","DOI":"10.1128\/IAI.73.11.7297-7303.2005","article-title":"Selection of protective epitopes for Brucella melitensis by DNA vaccination","volume":"73","author":"Yang","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0360","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1016\/j.biologicals.2012.01.002","article-title":"Efficacy evaluation of live Escherichia coli expression Brucella P39 protein combined with CpG oligodeoxynucleotides vaccine against Brucella melitensis 16M, in BALB\/c mice","volume":"40","author":"Al-Mariri","year":"2012","journal-title":"Biologicals"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0365","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/s11259-009-9337-x","article-title":"Protection of mice against Brucella abortus 544 challenge by vaccination with recombinant Omp28 adjuvanted with CpG oligonucleotides","volume":"34","author":"Kaushik","year":"2010","journal-title":"Vet Res Commun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0370","doi-asserted-by":"crossref","first-page":"4017","DOI":"10.1016\/j.vaccine.2012.04.036","article-title":"Invasive Escherichia coli vaccines expressing Brucella melitensis outer membrane proteins 31 or 16 or periplasmic protein BP26 confer protection in mice challenged with B. melitensis","volume":"30","author":"Gupta","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0375","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.vetmic.2012.07.016","article-title":"Vaccination with recombinant flagellar proteins FlgJ and FliN induce protection against Brucella abortus 544 infection in BALB\/c mice","volume":"161","author":"Li","year":"2012","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0380","doi-asserted-by":"crossref","first-page":"3663","DOI":"10.1016\/j.vaccine.2013.06.013","article-title":"Protective effect of a DNA vaccine containing an open reading frame with homology to an ABC-type transporter present in the genomic island 3 of Brucella abortus in BALB\/c mice","volume":"31","author":"Riquelme-Neira","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0385","doi-asserted-by":"crossref","first-page":"4537","DOI":"10.1016\/j.vaccine.2014.06.012","article-title":"Evaluation of immunogenicity and protective efficacy of a plasmid DNA vaccine encoding ribosomal protein L9 of Brucella abortus in BALB\/c mice","volume":"32","author":"Jain","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0390","doi-asserted-by":"crossref","first-page":"6659","DOI":"10.1016\/j.vaccine.2014.09.013","article-title":"Immunization of mice with a novel recombinant molecular chaperon confers protection against Brucella melitensis infection","volume":"32","author":"Ghasemi","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0395","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.molimm.2014.06.017","article-title":"Identification of a new immunogenic candidate conferring protection against Brucella melitensis infection in Mice","volume":"62","author":"Ghasemi","year":"2014","journal-title":"Mol Immunol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0400","doi-asserted-by":"crossref","first-page":"6129","DOI":"10.1016\/j.vaccine.2013.07.041","article-title":"The vaccine candidate BLSOmp31 protects mice against Brucella canis infection","volume":"31","author":"Clausse","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0405","doi-asserted-by":"crossref","first-page":"5889","DOI":"10.1016\/j.vaccine.2014.08.073","article-title":"Novel vector vaccine against Brucella abortus based on influenza A viruses expressing Brucella L7\/L12 or Omp16 proteins: evaluation of protection in pregnant heifers","volume":"32","author":"Tabynov","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0410","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1128\/IAI.01151-08","article-title":"Immunization with recombinant Brucella species outer membrane protein Omp16 or Omp19 in adjuvant induces specific CD4+ and CD8+ T cells as well as systemic and oral protection against Brucella abortus infection","volume":"77","author":"Pasquevich","year":"2009","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0415","doi-asserted-by":"crossref","first-page":"6721","DOI":"10.1016\/j.vaccine.2007.07.002","article-title":"Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB\/c mice","volume":"25","author":"Delpino","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0420","doi-asserted-by":"crossref","first-page":"2734","DOI":"10.1128\/IAI.74.5.2734-2741.2006","article-title":"Protective immunity elicited by a divalent DNA vaccine encoding both the L7\/L12 and Omp16 genes of Brucella abortus in BALB\/c mice","volume":"74","author":"Luo","year":"2006","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0425","doi-asserted-by":"crossref","first-page":"5238","DOI":"10.1128\/IAI.71.9.5238-5244.2003","article-title":"Purification and characterization of an immunogenic aminopeptidase of Brucella melitensis","volume":"71","author":"Contreras-Rodriguez","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0430","first-page":"192","article-title":"Immunogenicity assessment of Brucella mellitensis HSP and TF proteins by immunized rabbit serum","volume":"12","author":"Ghasemi","year":"2013","journal-title":"Iran J Allergy Asthma Immunol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0435","doi-asserted-by":"crossref","first-page":"e6875","DOI":"10.5812\/jjm.6875","article-title":"Optimization and efficient purification in production of Brucella melitensis recombinant HSP A and TF proteins with low endotoxin contents","volume":"6","author":"Ghasemi","year":"2013","journal-title":"Jundishapur J Microbiol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0440","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/0022-1759(90)90029-U","article-title":"Removal of endotoxin from protein solutions by phase separation using Triton X-114","volume":"132","author":"Aida","year":"1990","journal-title":"J Immunol Methods"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0445","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1002\/(SICI)1097-0290(19961020)52:2<185::AID-BIT1>3.0.CO;2-M","article-title":"Novel bioseparations using two-phase aqueous micellar systems","volume":"52","author":"Liu","year":"1996","journal-title":"Biotechnol Bioeng"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0450","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1016\/j.pep.2005.09.027","article-title":"Single step protocol to purify recombinant proteins with low endotoxin contents","volume":"46","author":"Reichelt","year":"2006","journal-title":"Protein Expr Purif"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0455","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/0076-6879(90)82008-P","article-title":"Quantitation of protein","volume":"182","author":"Stoscheck","year":"1990","journal-title":"Methods Enzymol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0460","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.molimm.2011.08.009","article-title":"Immunoproteomic analysis of Brucella melitensis and identification of a new immunogenic candidate protein for the development of brucellosis subunit vaccine","volume":"49","author":"Yang","year":"2011","journal-title":"Mol Immunol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0465","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1297-9716-43-29","article-title":"What have we learned from brucellosis in the mouse model?","volume":"43","author":"Grillo","year":"2012","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0470","doi-asserted-by":"crossref","first-page":"17","DOI":"10.3389\/fcimb.2013.00017","article-title":"Host\u2013Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis","volume":"3","author":"Gomez","year":"2013","journal-title":"Front Cell Inf Microbiol"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0475","doi-asserted-by":"crossref","first-page":"e16203","DOI":"10.1371\/journal.pone.0016203","article-title":"An oral vaccine based on U-Omp19 induces protection against B. abortus mucosal challenge by inducing an adaptive IL-17 immune response in mice","volume":"6","author":"Pasquevich","year":"2011","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0480","doi-asserted-by":"crossref","first-page":"4271","DOI":"10.1128\/IAI.00761-12","article-title":"Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice","volume":"80","author":"Vitry","year":"2012","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0485","doi-asserted-by":"crossref","first-page":"4899","DOI":"10.1128\/IAI.61.11.4899-4901.1993","article-title":"Endogenous gamma interferon mediates resistance to Brucella abortus infection","volume":"61","author":"Zhan","year":"1993","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0490","doi-asserted-by":"crossref","first-page":"8079","DOI":"10.1128\/IAI.73.12.8079-8088.2005","article-title":"Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection","volume":"73","author":"Cassataro","year":"2005","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0495","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.molmed.2007.12.007","article-title":"The wolf in sheep's clothing: the role of interleukin-6 in immunity, inflammation and cancer","volume":"14","author":"Naugler","year":"2008","journal-title":"Trends Mol Med"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0500","doi-asserted-by":"crossref","first-page":"6541","DOI":"10.1128\/IAI.69.10.6541-6544.2001","article-title":"Mouse cytokine profiles associated with Brucella abortus RB51 vaccination or B. abortus 2308 infection","volume":"69","author":"Pasquali","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0505","doi-asserted-by":"crossref","first-page":"5750","DOI":"10.1128\/IAI.71.10.5750-5755.2003","article-title":"Brucella lumazine synthase elicits a mixed Th1\u2013Th2 immune response and reduces infection in mice challenged with Brucella abortus 544 independently of the adjuvant formulation used","volume":"71","author":"Velikovsky","year":"2003","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2015.09.013_bib0510","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/S1286-4579(02)00075-8","article-title":"The recombinant Omp31 from Brucella melitensis alone or associated with rough lipopolysaccharide induces protection against Brucella ovis infection in BALB\/c mice","volume":"5","author":"Estein","year":"2003","journal-title":"Microbes Infect"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X15012712?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X15012712?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,8]],"date-time":"2020-09-08T11:10:16Z","timestamp":1599563416000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X15012712"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10]]},"references-count":51,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["S0264410X15012712"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2015.09.013","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2015,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Simultaneous immunization of mice with Omp31 and TF provides protection against Brucella melitensis infection","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2015.09.013","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Copyright \u00a9 2015 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}