{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T23:54:59Z","timestamp":1783814099223,"version":"3.55.0"},"reference-count":39,"publisher":"Elsevier BV","issue":"30","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"name":"Major Project of National Natural Science Foundation of China","award":["31430087"],"award-info":[{"award-number":["31430087"]}]},{"name":"Modern Agro-Industry Technology Research System","award":["nycytx-42-G3-01"],"award-info":[{"award-number":["nycytx-42-G3-01"]}]},{"name":"Application Technology Research and Development Fund of Harbin","award":["2014AB3AN058"],"award-info":[{"award-number":["2014AB3AN058"]}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2016,6]]},"DOI":"10.1016\/j.vaccine.2016.02.072","type":"journal-article","created":{"date-parts":[[2016,5,8]],"date-time":"2016-05-08T13:46:34Z","timestamp":1462715194000},"page":"3508-3514","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":20,"title":["An optimized, highly efficient, self-assembled, subvirus-like particle of infectious bursal disease virus (IBDV)"],"prefix":"10.1016","volume":"34","author":[{"given":"Miao","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honglei","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaole","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yulong","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2016.02.072_bib0200","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1080\/03079459108418748","article-title":"Acute infectious bursa disease in poultry: isolation and characterization of a highly virulent strain","volume":"20","author":"Van Den Berg","year":"1991","journal-title":"Avian Dis"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0205","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1099\/0022-1317-69-8-1757","article-title":"Biochemistry and immunology of infectious bursa disease virus","volume":"69","author":"Kibenge","year":"1988","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0210","series-title":"Diseases of poultry","first-page":"721","article-title":"Infectious bursal disease","author":"Lukert","year":"1997"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0215","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.vetmic.2003.08.005","article-title":"Research on infectious bursal disease \u2013 the past, the present and the future","volume":"97","author":"Muller","year":"2003","journal-title":"Vet Microbiol"},{"issue":"12","key":"10.1016\/j.vaccine.2016.02.072_bib0220","doi-asserted-by":"crossref","first-page":"5001","DOI":"10.1093\/nar\/14.12.5001","article-title":"Genomic structure of the large RNA segment of infectious bursa disease virus","volume":"14","author":"Hudson","year":"1986","journal-title":"Nucleic Acids Res"},{"issue":"1","key":"10.1016\/j.vaccine.2016.02.072_bib0225","doi-asserted-by":"crossref","first-page":"384","DOI":"10.1128\/JVI.44.1.384-392.1982","article-title":"Biosynthesis of virus-specific proteins in cells infected with infectious bursa disease virus and their significance as structural elements for infectious virus and incomplete particles","volume":"44","author":"Muller","year":"1982","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0230","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1099\/0022-1317-76-2-437","article-title":"Identification of a novel viral protein in infectious disease virus-infected cells","volume":"76","author":"Mundt","year":"1995","journal-title":"J Gen Virol"},{"issue":"22","key":"10.1016\/j.vaccine.2016.02.072_bib0235","doi-asserted-by":"crossref","first-page":"10815","DOI":"10.1128\/JVI.75.22.10815-10828.2001","article-title":"C terminus of infectious bursal disease virus major capsid protein VP2 is involved in definition of the T number for capsid assembly","volume":"75","author":"Caston","year":"2010","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0240","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.jsb.2006.02.014","article-title":"Crystal structure of infectious bursal disease virus VP2 subviral particle at 2.6A resolution: implications in virion assembly and immunogenicity","volume":"155","author":"Lee","year":"2006","journal-title":"J Struct Biol"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0245","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1016\/j.ymeth.2006.07.018","article-title":"Virus-like particles: passport to immune recognition","volume":"40","author":"Grgacic","year":"2006","journal-title":"Methods"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0250","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.1586\/erv.10.115","article-title":"Virus-like particles in vaccine development","volume":"9","author":"Roldao","year":"2010","journal-title":"Expert Rev Vaccines"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0255","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1099\/0022-1317-71-6-1303","article-title":"A comparison of the sequences of segment A of four infectious bursa disease virus strains and identification of a variable region in VP2","volume":"71","author":"Bayliss","year":"1990","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0260","doi-asserted-by":"crossref","first-page":"864","DOI":"10.1016\/j.vaccine.2008.11.085","article-title":"Effective protection by high efficiency bicistronic DNA vaccine against infectious bursal disease virus expressing VP2 protein and chicken IL-2","volume":"27","author":"Kumar","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0265","doi-asserted-by":"crossref","first-page":"753","DOI":"10.2307\/1592294","article-title":"Insect cell-derived VP2 of infectious bursal disease virus confers protection against the disease in chickens","volume":"40","author":"Pitcovski","year":"1996","journal-title":"Avian Dis"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0270","doi-asserted-by":"crossref","first-page":"4844","DOI":"10.1016\/j.vaccine.2005.05.015","article-title":"Development of recombinant VP2 vaccine for the prevention of infectious bursal disease of chickens","volume":"23","author":"Rong","year":"2005","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0275","doi-asserted-by":"crossref","first-page":"3990","DOI":"10.1016\/j.vaccine.2010.03.021","article-title":"Protection of chickens, with or without maternal antibodies, against IBDV infection by a recombinant IBDV-VP2 protein","volume":"28","author":"Zhou","year":"2010","journal-title":"Vaccine"},{"issue":"December (12)","key":"10.1016\/j.vaccine.2016.02.072_bib0280","doi-asserted-by":"crossref","first-page":"e83210","DOI":"10.1371\/journal.pone.0083210","article-title":"Protective oral vaccination against infectious bursal disease virus using the major viral antigenic protein VP2 produced in Pichia pastoris","volume":"8","author":"Taghavian","year":"2013","journal-title":"PLoS ONE"},{"issue":"23","key":"10.1016\/j.vaccine.2016.02.072_bib0285","doi-asserted-by":"crossref","first-page":"3342","DOI":"10.1016\/S0264-410X(02)00804-6","article-title":"Structure-dependent efficacy of infectious bursal disease virus (IBDV) recombinant vaccines","volume":"21","author":"Martinez-Torrecuadrada","year":"2003","journal-title":"Vaccine"},{"issue":"October (1)","key":"10.1016\/j.vaccine.2016.02.072_bib0290","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1186\/s12985-015-0403-4","article-title":"Efficient self-assembly and protective efficacy of infectious bursal disease virus-like particles by a recombinant baculovirus co-expressing precursor polyprotein and VP4","volume":"12","author":"Lee","year":"2015","journal-title":"Virol J"},{"issue":"1","key":"10.1016\/j.vaccine.2016.02.072_bib0295","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1637\/10312-080212-Reg.1","article-title":"Multivalent virus-like-particle vaccine protects against classic and variant infectious bursal disease viruses","volume":"57","author":"Jackwood","year":"2013","journal-title":"Avian Dis"},{"issue":"June (2)","key":"10.1016\/j.vaccine.2016.02.072_bib0300","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1637\/9597-111210-Reg.1","article-title":"Baculovirus virions displaying infectious bursal disease virus VP2 protein protect chickens against infectious bursal disease virus infection","volume":"55","author":"Xu","year":"2011","journal-title":"Avian Dis"},{"issue":"Apr (1)","key":"10.1016\/j.vaccine.2016.02.072_bib0305","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.virol.2004.01.025","article-title":"The C-terminal domain of the pVP2 precursor is essential for the interaction between VP2 and VP3, the capsid polypeptides of infectious bursal disease virus","volume":"322","author":"O\u00f1a","year":"2004","journal-title":"Virology"},{"issue":"August (3\u20134)","key":"10.1016\/j.vaccine.2016.02.072_bib0310","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.vetimm.2012.06.019","article-title":"Protective immune responses of recombinant VP2 subunit antigen of infectious bursal disease virus in chickens","volume":"148","author":"Pradhan","year":"2012","journal-title":"Vet Immunol Immunopathol"},{"issue":"March (12)","key":"10.1016\/j.vaccine.2016.02.072_bib0315","doi-asserted-by":"crossref","first-page":"2125","DOI":"10.1016\/j.vaccine.2012.01.040","article-title":"Virus-like particles of hepatitis B virus core protein containing five mimotopes of infectious bursal disease virus (IBDV) protect chickens against IBDV","volume":"30","author":"Wang","year":"2012","journal-title":"Vaccine"},{"issue":"September (3)","key":"10.1016\/j.vaccine.2016.02.072_bib0320","doi-asserted-by":"crossref","first-page":"241","DOI":"10.4142\/jvs.2006.7.3.241","article-title":"Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus","volume":"7","author":"Omar","year":"2006","journal-title":"J Vet Sci"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0325","doi-asserted-by":"crossref","first-page":"549","DOI":"10.1016\/0264-410X(90)90006-8","article-title":"Passive protection against infectious bursal disease virus by viral VP2 expressed in yeast","volume":"8","author":"Macreadie","year":"1990","journal-title":"Vaccine"},{"issue":"9","key":"10.1016\/j.vaccine.2016.02.072_bib0330","doi-asserted-by":"crossref","first-page":"e42870","DOI":"10.1371\/journal.pone.0042870","article-title":"Protective vaccination against infectious bursal disease virus with whole recombinant Kluyveromyces lactis yeast expressing the viral VP2 subunit","volume":"7","author":"Arnold","year":"2012","journal-title":"PLoS ONE"},{"issue":"May (23)","key":"10.1016\/j.vaccine.2016.02.072_bib0335","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1016\/j.vaccine.2013.03.064","article-title":"Transient expression of VP2 in Nicotiana benthamiana and its use as a plant-based vaccine against infectious bursal disease virus","volume":"31","author":"G\u00f3mez","year":"2013","journal-title":"Vaccine"},{"issue":"May (2)","key":"10.1016\/j.vaccine.2016.02.072_bib0340","doi-asserted-by":"crossref","first-page":"352","DOI":"10.1006\/viro.1999.9641","article-title":"Protection of chickens against very virulent infectious bursal disease virus (IBDV) and Marek's disease virus (MDV) with a recombinant MDV expressing IBDV VP2","volume":"257","author":"Tsukamoto","year":"1999","journal-title":"Virology"},{"issue":"October","key":"10.1016\/j.vaccine.2016.02.072_bib0345","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.rvsc.2015.07.004","article-title":"Development and evaluation of a Salmonella typhimurium flagellin based chimeric DNA vaccine against infectious bursal disease of poultry","volume":"102","author":"Deb","year":"2015","journal-title":"Res Vet Sci"},{"issue":"June (1\u20132)","key":"10.1016\/j.vaccine.2016.02.072_bib0350","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.vetmic.2014.02.019","article-title":"DNA vaccination with VP2 gene fragment confers protection against Infectious Bursal Disease Virus in chickens","volume":"171","author":"Pradhan","year":"2014","journal-title":"Vet Microbiol"},{"issue":"December (1\u20132)","key":"10.1016\/j.vaccine.2016.02.072_bib0355","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.jviromet.2005.06.002","article-title":"Purification of capsid-like particles of infectious bursal disease virus (IBDV) VP2 expressed in E. coli with a metal-ion affinity membrane system","volume":"130","author":"Chen","year":"2005","journal-title":"J Virol Methods"},{"issue":"1","key":"10.1016\/j.vaccine.2016.02.072_bib0360","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1574-6976.2000.tb00532.x","article-title":"Heterologous protein expression in the methylotrophic yeast Pichia pastoris","volume":"24","author":"Cereghino","year":"2000","journal-title":"FEMS Microbiol Rev"},{"issue":"Mar\u2013April (2)","key":"10.1016\/j.vaccine.2016.02.072_bib0365","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1002\/jmr.687","article-title":"Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production","volume":"18","author":"Daly","year":"2005","journal-title":"J Mol Recognit"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0370","doi-asserted-by":"crossref","first-page":"2273","DOI":"10.1016\/j.vaccine.2012.01.075","article-title":"Recombinant gp90 protein expressed in Pichia pastoris induces a protective immune response against reticuloendotheliosis virus in chickens","volume":"30","author":"Li","year":"2012","journal-title":"Vaccine"},{"issue":"April (1)","key":"10.1016\/j.vaccine.2016.02.072_bib0375","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1016\/j.jviromet.2008.11.020","article-title":"Formation of subviral particles of the capsid protein VP2 of infectious bursal disease virus and its application in serological diagnosis","volume":"157","author":"Dey","year":"2009","journal-title":"J Virol Methods"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0380","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.copbio.2007.10.013","article-title":"Virus-like particles \u2013 universal molecular toolboxes","volume":"18","author":"Ludwig","year":"2007","journal-title":"Curr Opin Biotechnol"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0385","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.molimm.2004.06.028","article-title":"Recombinant HIV-1 Pr55gag virus-like particles: potent stimulators of innate and acquired immune responses","volume":"42","author":"Deml","year":"2005","journal-title":"Mol Immunol"},{"key":"10.1016\/j.vaccine.2016.02.072_bib0390","doi-asserted-by":"crossref","first-page":"2361","DOI":"10.4049\/jimmunol.178.4.2361","article-title":"Insect baculoviruses strongly potentiate adaptive immune responses by inducing type I IFN","volume":"178","author":"Hervas-Stubbs","year":"2007","journal-title":"J Immunol"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X16002772?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X16002772?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,19]],"date-time":"2020-09-19T15:29:16Z","timestamp":1600529356000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X16002772"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6]]},"references-count":39,"journal-issue":{"issue":"30","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["S0264410X16002772"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2016.02.072","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2016,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"An optimized, highly efficient, self-assembled, subvirus-like particle of infectious bursal disease virus (IBDV)","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2016.02.072","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}