{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T13:43:27Z","timestamp":1759412607158},"reference-count":47,"publisher":"Elsevier BV","issue":"32","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2016,7]]},"DOI":"10.1016\/j.vaccine.2016.05.030","type":"journal-article","created":{"date-parts":[[2016,5,21]],"date-time":"2016-05-21T23:50:21Z","timestamp":1463874621000},"page":"3634-3640","update-policy":"http:\/\/dx.doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":21,"title":["A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs"],"prefix":"10.1016","volume":"34","author":[{"given":"Marie","family":"Borggren","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jens","family":"Nielsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingrid","family":"Karlsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina S.","family":"Dalgaard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramona","family":"Trebbien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Fomsgaard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2016.05.030_bib0240","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3390\/vaccines3010022","article-title":"Optimal use of vaccines for control of influenza A virus in swine","volume":"3","author":"Sandbulte","year":"2015","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0245","series-title":"Diseases of swine","first-page":"469","article-title":"Swine influenza","author":"Olsen","year":"2006"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0250","doi-asserted-by":"crossref","first-page":"931","DOI":"10.1038\/nature08157","article-title":"Emergence and pandemic potential of swine-origin H1N1 influenza virus","volume":"459","author":"Neumann","year":"2009","journal-title":"Nature"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0255","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1016\/0264-410X(93)90385-B","article-title":"Protection against a lethal influenza virus challenge by immunization with a haemagglutinin-expressing plasmid DNA","volume":"11","author":"Robinson","year":"1993","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0260","doi-asserted-by":"crossref","first-page":"1745","DOI":"10.1126\/science.8456302","article-title":"Heterologous protection against influenza by injection of DNA encoding a viral protein","volume":"259","author":"Ulmer","year":"1993","journal-title":"Science"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0265","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1099\/0022-1317-83-8-1851","article-title":"Vaccination of pigs with a DNA construct expressing an influenza virus M2-nucleoprotein fusion protein exacerbates disease after challenge with influenza A virus","volume":"83","author":"Heinen","year":"2002","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0270","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1006\/jsre.1998.5325","article-title":"In vivo gene transfer to skin and wound by microseeding","volume":"78","author":"Eriksson","year":"1998","journal-title":"J Surg Res"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0275","doi-asserted-by":"crossref","first-page":"1491","DOI":"10.1128\/JVI.72.2.1491-1496.1998","article-title":"Immunization of pigs with a particle-mediated DNA vaccine to influenza A virus protects against challenge with homologous virus","volume":"72","author":"Macklin","year":"1998","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0280","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.jviromet.2011.02.008","article-title":"Strategies for improving the efficacy of a H6 subtype avian influenza DNA vaccine in chickens","volume":"173","author":"Shan","year":"2011","journal-title":"J Virol Methods"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0285","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1637\/10150-040812-ResNote.1","article-title":"Protective efficacy of an H5N1 DNA vaccine against challenge with a lethal H5N1 virus in quail","volume":"56","author":"Li","year":"2012","journal-title":"Avian Dis"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0290","doi-asserted-by":"crossref","first-page":"e28757","DOI":"10.1371\/journal.pone.0028757","article-title":"Polyvalent DNA vaccines expressing HA antigens of H5N1 influenza viruses with an optimized leader sequence elicit cross-protective antibody responses","volume":"6","author":"Wang","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0295","doi-asserted-by":"crossref","first-page":"3965","DOI":"10.1016\/j.vaccine.2012.03.026","article-title":"Immunogenicity and clinical protection against equine influenza by DNA vaccination of ponies","volume":"30","author":"Ault","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0300","doi-asserted-by":"crossref","first-page":"2506","DOI":"10.1016\/j.vaccine.2009.02.061","article-title":"DNA vaccination protects against an influenza challenge in a double-blind randomised placebo-controlled phase 1b clinical trial","volume":"27","author":"Jones","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0305","doi-asserted-by":"crossref","first-page":"2565","DOI":"10.1016\/j.vaccine.2010.01.029","article-title":"Phase 1 clinical trials of the safety and immunogenicity of adjuvanted plasmid DNA vaccines encoding influenza A virus H5 hemagglutinin","volume":"28","author":"Smith","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0310","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.1128\/CVI.05171-11","article-title":"DNA vaccination elicits protective immune responses against pandemic and classic swine influenza viruses in pigs","volume":"18","author":"Gorres","year":"2011","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0315","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1016\/j.vaccine.2003.11.073","article-title":"Enhancement of DNA vaccine potency in rhesus macaques by electroporation","volume":"22","author":"Otten","year":"2004","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0320","doi-asserted-by":"crossref","first-page":"4635","DOI":"10.4049\/jimmunol.164.9.4635","article-title":"Increased DNA vaccine delivery and immunogenicity by electroporation in vivo","volume":"164","author":"Widera","year":"2000","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0325","doi-asserted-by":"crossref","first-page":"225","DOI":"10.3390\/vaccines1030225","article-title":"Vector design for improved DNA vaccine efficacy, safety and production","volume":"1","author":"Williams","year":"2013","journal-title":"Vaccines"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0330","doi-asserted-by":"crossref","first-page":"916","DOI":"10.1016\/S1473-3099(11)70240-7","article-title":"DNA priming and influenza vaccine immunogenicity: two phase 1 open label randomised clinical trials","volume":"11","author":"Ledgerwood","year":"2011","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0335","doi-asserted-by":"crossref","first-page":"2281","DOI":"10.1016\/j.vaccine.2013.02.061","article-title":"A polyvalent influenza A DNA vaccine induces heterologous immunity and protects pigs against pandemic A(H1N1)pdm09 virus infection","volume":"31","author":"Bragstad","year":"2013","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0340","doi-asserted-by":"crossref","first-page":"2833","DOI":"10.1016\/j.vaccine.2014.02.038","article-title":"Protective immunity to H7N9 influenza viruses elicited by synthetic DNA vaccine","volume":"32","author":"Yan","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0345","doi-asserted-by":"crossref","first-page":"5648","DOI":"10.1128\/JVI.72.7.5648-5653.1998","article-title":"Protective CD4+ and CD8+ T cells against influenza virus induced by vaccination with nucleoprotein DNA","volume":"72","author":"Ulmer","year":"1998","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0350","doi-asserted-by":"crossref","first-page":"2842","DOI":"10.1016\/S0264-410X(01)00014-7","article-title":"Immunization of pigs against influenza virus infection by DNA vaccine priming followed by killed-virus vaccine boosting","volume":"19","author":"Larsen","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0355","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0378-1135(00)00175-9","article-title":"DNA vaccination against influenza viruses: a review with emphasis on equine and swine influenza","volume":"74","author":"Olsen","year":"2000","journal-title":"Vet Microbiol"},{"issue":"8","key":"10.1016\/j.vaccine.2016.05.030_bib0360","doi-asserted-by":"crossref","first-page":"1983","DOI":"10.1080\/21645515.2015.1011987","article-title":"Vector optimization and needle-free intradermal application of a broadly protective polyvalent influenza A DNA vaccine for pigs and humans","volume":"11","author":"Borggren","year":"2015","journal-title":"Hum Vaccines Immunother"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0365","unstructured":"WHO Recommendations for Influenza Vaccine Composition [http:\/\/www.who.int\/influenza\/vaccines\/virus\/en\/]."},{"key":"10.1016\/j.vaccine.2016.05.030_bib0370","unstructured":"World Health Organization Global Influenza Surveillance Network. Manual for the laboratory diagnosis and virological surveillance of influenza http:\/\/wwwwhoint\/influenza\/gisrs_laboratory\/manual_diagnosis_surveillance_influenza\/en\/.2011."},{"key":"10.1016\/j.vaccine.2016.05.030_bib0375","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1128\/CMR.00097-12","article-title":"Traditional and new influenza vaccines","volume":"26","author":"Wong","year":"2013","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0380","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1002\/rmv.1713","article-title":"Contribution of antibody production against neuraminidase to the protection afforded by influenza vaccines","volume":"22","author":"Marcelin","year":"2012","journal-title":"Rev Med Virol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0385","doi-asserted-by":"crossref","first-page":"1487","DOI":"10.1128\/JVI.64.4.1487-1497.1990","article-title":"Evolution of the nucleoprotein gene of influenza A virus","volume":"64","author":"Gorman","year":"1990","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0390","doi-asserted-by":"crossref","first-page":"4168","DOI":"10.4049\/jimmunol.181.6.4168","article-title":"A novel role for non-neutralizing antibodies against nucleoprotein in facilitating resistance to influenza virus","volume":"181","author":"Carragher","year":"2008","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0395","doi-asserted-by":"crossref","first-page":"5027","DOI":"10.1128\/JVI.00150-11","article-title":"Regulation of antinucleoprotein IgG by systemic vaccination and its effect on influenza virus clearance","volume":"85","author":"Lamere","year":"2011","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0400","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1128\/JVI.56.2.502-511.1985","article-title":"Characterization of the influenza virus M2 integral membrane protein and expression at the infected-cell surface from cloned cDNA","volume":"56","author":"Zebedee","year":"1985","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0405","doi-asserted-by":"crossref","first-page":"2697","DOI":"10.1099\/0022-1317-82-11-2697","article-title":"Respiratory and systemic humoral and cellular immune responses of pigs to a heterosubtypic influenza A virus infection","volume":"82","author":"Heinen","year":"2001","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0410","doi-asserted-by":"crossref","first-page":"48","DOI":"10.3201\/eid1201.051237","article-title":"Cell-mediated protection in influenza infection","volume":"12","author":"Thomas","year":"2006","journal-title":"Emer Infect Dis"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0415","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1016\/S0264-410X(96)00280-0","article-title":"Immunization of non-human primates with DNA vaccines","volume":"15","author":"Liu","year":"1997","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0420","doi-asserted-by":"crossref","first-page":"3681","DOI":"10.1016\/S0264-410X(01)00078-0","article-title":"Protective immunity against influenza A virus induced by immunization with DNA plasmid containing influenza M gene","volume":"19","author":"Okuda","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0425","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1186\/s13567-015-0182-3","article-title":"Magnitude and kinetics of multifunctional CD4+ and CD8beta+ T cells in pigs infected with swine influenza A virus","volume":"46","author":"Talker","year":"2015","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0430","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1111\/j.1750-2659.2010.00177.x","article-title":"Pandemic influenza 1918 H1N1 and 1968 H3N2 DNA vaccines induce cross-reactive immunity in ferrets against infection with viruses drifted for decades","volume":"5","author":"Bragstad","year":"2011","journal-title":"Influenza Other Respir Viruses"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0435","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1017\/S146625231000006X","article-title":"Swine influenza vaccines: current status and future perspectives","volume":"11","author":"Ma","year":"2010","journal-title":"Anim Health Res Rev\/Conf Res Workers Anim Dis"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0440","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.virusres.2004.02.023","article-title":"Genetic relationships, serological cross-reaction and cross-protection between H1N2 and other influenza A virus subtypes endemic in European pigs","volume":"103","author":"Reeth","year":"2004","journal-title":"Virus Res"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0445","doi-asserted-by":"crossref","first-page":"1375","DOI":"10.1016\/S0264-410X(02)00688-6","article-title":"Protection against a European H1N2 swine influenza virus in pigs previously infected with H1N1 and\/or H3N2 subtypes","volume":"21","author":"Van Reeth","year":"2003","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0450","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/S0165-2427(03)00019-9","article-title":"Effect of maternally derived antibodies on the clinical signs and immune response in pigs after primary and secondary infection with an influenza H1N1 virus","volume":"92","author":"Loeffen","year":"2003","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0455","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.vetimm.2011.03.019","article-title":"The influence of age and maternal antibodies on the postvaccinal response against swine influenza viruses in pigs","volume":"142","author":"Markowska-Daniel","year":"2011","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0460","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.vetimm.2006.02.008","article-title":"The immune response and maternal antibody interference to a heterologous H1N1 swine influenza virus infection following vaccination","volume":"112","author":"Kitikoon","year":"2006","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0465","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.vetmic.2007.07.011","article-title":"Failure of protection and enhanced pneumonia with a US H1N2 swine influenza virus in pigs vaccinated with an inactivated classical swine H1N1 vaccine","volume":"126","author":"Vincent","year":"2008","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2016.05.030_bib0470","doi-asserted-by":"crossref","first-page":"1732","DOI":"10.1016\/S0264-410X(02)00736-3","article-title":"DNA vaccination of neonate piglets in the face of maternal immunity induces humoral memory and protection against a virulent pseudorabies virus challenge","volume":"21","author":"Fischer","year":"2003","journal-title":"Vaccine"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X16303206?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X16303206?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,9,20]],"date-time":"2020-09-20T15:17:33Z","timestamp":1600615053000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X16303206"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7]]},"references-count":47,"journal-issue":{"issue":"32","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["S0264410X16303206"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2016.05.030","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2016,7]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"A polyvalent influenza DNA vaccine applied by needle-free intradermal delivery induces cross-reactive humoral and cellular immune responses in pigs","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2016.05.030","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The Author(s). Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}