{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:45:56Z","timestamp":1790397956609,"version":"4.1.0"},"reference-count":45,"publisher":"Elsevier BV","issue":"42","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI 111451"],"award-info":[{"award-number":["AI 111451"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI 109925"],"award-info":[{"award-number":["AI 109925"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.com.au","clinicalkey.fr","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2016,9]]},"DOI":"10.1016\/j.vaccine.2016.08.061","type":"journal-article","created":{"date-parts":[[2016,9,10]],"date-time":"2016-09-10T00:45:06Z","timestamp":1473468306000},"page":"5090-5098","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":39,"title":["M2SR, a novel live single replication influenza virus vaccine, provides effective heterosubtypic protection in mice"],"prefix":"10.1016","volume":"34","author":[{"given":"Sally","family":"Sarawar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Hatta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Dias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriele","family":"Neumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Kawaoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamuk","family":"Bilsel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"2","key":"10.1016\/j.vaccine.2016.08.061_b0005","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1093\/aje\/kwj024","article-title":"Mortality due to influenza in the United States\u2013an annualized regression approach using multiple-cause mortality data","volume":"163","author":"Dushoff","year":"2006","journal-title":"Am J Epidemiol"},{"issue":"1","key":"10.1016\/j.vaccine.2016.08.061_b0010","first-page":"10","article-title":"Early estimates of seasonal influenza vaccine effectiveness \u2013 United States, January 2015","volume":"64","author":"Flannery","year":"2015","journal-title":"MMWR Morb Mortal Wkly Rep"},{"issue":"2","key":"10.1016\/j.vaccine.2016.08.061_b0015","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1001\/jama.282.2.137","article-title":"Effectiveness of live, attenuated intranasal influenza virus vaccine in healthy, working adults: a randomized controlled trial","volume":"282","author":"Nichol","year":"1999","journal-title":"JAMA"},{"issue":"9","key":"10.1016\/j.vaccine.2016.08.061_b0020","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1016\/j.vaccine.2009.11.068","article-title":"Efficacy of live attenuated influenza vaccine in children against influenza B viruses by lineage and antigenic similarity","volume":"28","author":"Belshe","year":"2010","journal-title":"Vaccine"},{"issue":"36","key":"10.1016\/j.vaccine.2016.08.061_b0025","doi-asserted-by":"crossref","DOI":"10.2807\/1560-7917.ES.2015.20.36.30013","article-title":"Effectiveness of seasonal influenza vaccine in preventing laboratory-confirmed influenza in primary care in the United Kingdom: 2014\/15 end of season results","volume":"20","author":"Pebody","year":"2015","journal-title":"Euro Surveill"},{"issue":"9","key":"10.1016\/j.vaccine.2016.08.061_b0030","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1001\/jama.2009.265","article-title":"Live attenuated or inactivated influenza vaccines and medical encounters for respiratory illnesses among US military personnel","volume":"301","author":"Wang","year":"2009","journal-title":"JAMA"},{"issue":"7","key":"10.1016\/j.vaccine.2016.08.061_b0035","doi-asserted-by":"crossref","first-page":"e2574","DOI":"10.1371\/journal.pone.0002574","article-title":"Baseline levels of influenza-specific CD4 memory T-cells affect T-cell responses to influenza vaccines","volume":"3","author":"He","year":"2008","journal-title":"PLoS ONE"},{"issue":"23","key":"10.1016\/j.vaccine.2016.08.061_b0040","doi-asserted-by":"crossref","first-page":"11756","DOI":"10.1128\/JVI.01460-06","article-title":"Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines","volume":"80","author":"He","year":"2006","journal-title":"J Virol"},{"issue":"1","key":"10.1016\/j.vaccine.2016.08.061_b0045","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/S1473-3099(11)70295-X","article-title":"Efficacy and effectiveness of influenza vaccines: a systematic review and meta-analysis","volume":"12","author":"Osterholm","year":"2012","journal-title":"Lancet Infect Dis"},{"issue":"3","key":"10.1016\/j.vaccine.2016.08.061_b0050","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1016\/0092-8674(92)90452-I","article-title":"Influenza virus M2 protein has ion channel activity","volume":"69","author":"Pinto","year":"1992","journal-title":"Cell"},{"issue":"4","key":"10.1016\/j.vaccine.2016.08.061_b0055","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1016\/0092-8674(92)90219-3","article-title":"Unpacking the incoming influenza virus","volume":"69","author":"Helenius","year":"1992","journal-title":"Cell"},{"issue":"6","key":"10.1016\/j.vaccine.2016.08.061_b0060","doi-asserted-by":"crossref","first-page":"3595","DOI":"10.1128\/JVI.79.6.3595-3605.2005","article-title":"The influenza A virus M2 cytoplasmic tail is required for infectious virus production and efficient genome packaging","volume":"79","author":"McCown","year":"2005","journal-title":"J Virol"},{"issue":"16","key":"10.1016\/j.vaccine.2016.08.061_b0065","doi-asserted-by":"crossref","first-page":"8178","DOI":"10.1128\/JVI.00627-06","article-title":"Distinct domains of the influenza a virus M2 protein cytoplasmic tail mediate binding to the M1 protein and facilitate infectious virus production","volume":"80","author":"McCown","year":"2006","journal-title":"J Virol"},{"issue":"11","key":"10.1016\/j.vaccine.2016.08.061_b0070","doi-asserted-by":"crossref","first-page":"5233","DOI":"10.1128\/JVI.00049-06","article-title":"The cytoplasmic tail of the influenza A virus M2 protein plays a role in viral assembly","volume":"80","author":"Iwatsuki-Horimoto","year":"2006","journal-title":"J Virol"},{"issue":"11","key":"10.1016\/j.vaccine.2016.08.061_b0075","doi-asserted-by":"crossref","first-page":"5947","DOI":"10.1128\/JVI.00450-09","article-title":"Influenza A virus lacking M2 protein as a live attenuated vaccine","volume":"83","author":"Watanabe","year":"2009","journal-title":"J Virol"},{"issue":"5","key":"10.1016\/j.vaccine.2016.08.061_b0080","doi-asserted-by":"crossref","first-page":"2486","DOI":"10.1128\/JVI.01899-07","article-title":"Novel approach to the development of effective H5N1 influenza A virus vaccines: use of M2 cytoplasmic tail mutants","volume":"82","author":"Watanabe","year":"2008","journal-title":"J Virol"},{"issue":"12","key":"10.1016\/j.vaccine.2016.08.061_b0085","doi-asserted-by":"crossref","first-page":"2308","DOI":"10.1016\/j.vaccine.2011.01.023","article-title":"An M2 cytoplasmic tail mutant as a live attenuated influenza vaccine against pandemic (H1N1) 2009 influenza virus","volume":"29","author":"Hatta","year":"2011","journal-title":"Vaccine"},{"issue":"16","key":"10.1016\/j.vaccine.2016.08.061_b0090","doi-asserted-by":"crossref","first-page":"9345","DOI":"10.1073\/pnas.96.16.9345","article-title":"Generation of influenza A viruses entirely from cloned cDNAs","volume":"96","author":"Neumann","year":"1999","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"12","key":"10.1016\/j.vaccine.2016.08.061_b0095","doi-asserted-by":"crossref","first-page":"2275","DOI":"10.1007\/s007050170002","article-title":"Universal primer set for the full-length amplification of all influenza A viruses","volume":"146","author":"Hoffmann","year":"2001","journal-title":"Arch Virol"},{"issue":"10","key":"10.1016\/j.vaccine.2016.08.061_b0100","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1038\/nm1477","article-title":"Fatal outcome of human influenza A (H5N1) is associated with high viral load and hypercytokinemia","volume":"12","author":"de Jong","year":"2006","journal-title":"Nat Med"},{"issue":"5","key":"10.1016\/j.vaccine.2016.08.061_b0105","doi-asserted-by":"crossref","first-page":"3112","DOI":"10.1128\/JVI.68.5.3112-3119.1994","article-title":"Concurrent production of interleukin-2, interleukin-10, and gamma interferon in the regional lymph nodes of mice with influenza pneumonia","volume":"68","author":"Sarawar","year":"1994","journal-title":"J Virol"},{"issue":"7","key":"10.1016\/j.vaccine.2016.08.061_b0110","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1093\/cid\/cis574","article-title":"Effectiveness of seasonal influenza vaccines in the United States during a season with circulation of all three vaccine strains","volume":"55","author":"Treanor","year":"2012","journal-title":"Clin Infect Dis"},{"issue":"24","key":"10.1016\/j.vaccine.2016.08.061_b0115","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1056\/NEJMoa061850","article-title":"Prevention of antigenically drifted influenza by inactivated and live attenuated vaccines","volume":"355","author":"Ohmit","year":"2006","journal-title":"N Engl J Med"},{"issue":"2","key":"10.1016\/j.vaccine.2016.08.061_b0120","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/S0022-3476(00)70097-7","article-title":"Efficacy of vaccination with live attenuated, cold-adapted, trivalent, intranasal influenza virus vaccine against a variant (A\/Sydney) not contained in the vaccine","volume":"136","author":"Belshe","year":"2000","journal-title":"J Pediatr"},{"issue":"13","key":"10.1016\/j.vaccine.2016.08.061_b0125","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1056\/NEJMoa0808652","article-title":"Comparative efficacy of inactivated and live attenuated influenza vaccines","volume":"361","author":"Monto","year":"2009","journal-title":"N Engl J Med"},{"issue":"2","key":"10.1016\/j.vaccine.2016.08.061_b0130","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.4049\/jimmunol.178.2.1030","article-title":"Priming with cold-adapted influenza A does not prevent infection but elicits long-lived protection against supralethal challenge with heterosubtypic virus","volume":"178","author":"Powell","year":"2007","journal-title":"J Immunol"},{"issue":"12","key":"10.1016\/j.vaccine.2016.08.061_b0135","doi-asserted-by":"crossref","first-page":"7437","DOI":"10.4049\/jimmunol.166.12.7437","article-title":"Heterosubtypic immunity to influenza A virus in mice lacking IgA, all Ig, NKT cells, or gamma delta T cells","volume":"166","author":"Benton","year":"2001","journal-title":"J Immunol"},{"issue":"23","key":"10.1016\/j.vaccine.2016.08.061_b0140","doi-asserted-by":"crossref","first-page":"3212","DOI":"10.1016\/S0264-410X(03)00234-2","article-title":"Intranasal immunization with formalin-inactivated virus vaccine induces a broad spectrum of heterosubtypic immunity against influenza A virus infection in mice","volume":"21","author":"Takada","year":"2003","journal-title":"Vaccine"},{"issue":"11","key":"10.1016\/j.vaccine.2016.08.061_b0145","doi-asserted-by":"crossref","first-page":"5141","DOI":"10.1128\/JVI.75.11.5141-5150.2001","article-title":"Mucosal delivery of inactivated influenza vaccine induces B-cell-dependent heterosubtypic cross-protection against lethal influenza A H5N1 virus infection","volume":"75","author":"Tumpey","year":"2001","journal-title":"J Virol"},{"issue":"9","key":"10.1016\/j.vaccine.2016.08.061_b0150","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1086\/521304","article-title":"Cross-protection against H5N1 influenza virus infection is afforded by intranasal inoculation with seasonal trivalent inactivated influenza vaccine","volume":"196","author":"Ichinohe","year":"2007","journal-title":"J Infect Dis"},{"issue":"44","key":"10.1016\/j.vaccine.2016.08.061_b0155","doi-asserted-by":"crossref","first-page":"7849","DOI":"10.1016\/j.vaccine.2011.07.093","article-title":"Live attenuated influenza vaccine (LAIV) impacts innate and adaptive immune responses","volume":"29","author":"Lanthier","year":"2011","journal-title":"Vaccine"},{"issue":"5","key":"10.1016\/j.vaccine.2016.08.061_b0160","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.micinf.2011.01.014","article-title":"The contribution of animal models to the understanding of the host range and virulence of influenza A viruses","volume":"13","author":"O\u2019Donnell","year":"2011","journal-title":"Microbes Infect"},{"issue":"4","key":"10.1016\/j.vaccine.2016.08.061_b0165","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.4049\/jimmunol.152.4.1653","article-title":"Heterosubtypic immunity to influenza type A virus in mice. Effector mechanisms and their longevity","volume":"152","author":"Liang","year":"1994","journal-title":"J Immunol"},{"issue":"3","key":"10.1016\/j.vaccine.2016.08.061_b0170","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.4049\/jimmunol.158.3.1222","article-title":"Mechanisms of heterosubtypic immunity to lethal influenza A virus infection in fully immunocompetent, T cell-depleted, beta2-microglobulin-deficient, and J chain-deficient mice","volume":"158","author":"Epstein","year":"1997","journal-title":"J Immunol"},{"issue":"3","key":"10.1016\/j.vaccine.2016.08.061_b0175","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1086\/318084","article-title":"Heterosubtypic immunity to influenza A virus infection requires B cells but not CD8+ cytotoxic T lymphocytes","volume":"183","author":"Nguyen","year":"2001","journal-title":"J Infect Dis"},{"issue":"1","key":"10.1016\/j.vaccine.2016.08.061_b0180","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1006\/viro.1998.9521","article-title":"Heterosubtypic immunity to lethal influenza A virus infection is associated with virus-specific CD8(+) cytotoxic T lymphocyte responses induced in mucosa-associated tissues","volume":"254","author":"Nguyen","year":"1999","journal-title":"Virology"},{"issue":"10","key":"10.1016\/j.vaccine.2016.08.061_b0185","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1038\/nm.3350","article-title":"Cellular immune correlates of protection against symptomatic pandemic influenza","volume":"19","author":"Sridhar","year":"2013","journal-title":"Nat Med"},{"issue":"2","key":"10.1016\/j.vaccine.2016.08.061_b0190","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1038\/nm.2612","article-title":"Preexisting influenza-specific CD4+ T cells correlate with disease protection against influenza challenge in humans","volume":"18","author":"Wilkinson","year":"2012","journal-title":"Nat Med"},{"key":"10.1016\/j.vaccine.2016.08.061_b0195","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1007\/978-1-4939-2963-4_11","article-title":"Cell-mediated immune response to influenza using ex vivo stimulation and assays of cytokine and granzyme B responses","volume":"1343","author":"McElhaney","year":"2015","journal-title":"Methods Mol Biol"},{"issue":"18","key":"10.1016\/j.vaccine.2016.08.061_b0200","doi-asserted-by":"crossref","first-page":"2418","DOI":"10.1016\/j.vaccine.2009.01.136","article-title":"Granzyme B: correlates with protection and enhanced CTL response to influenza vaccination in older adults","volume":"27","author":"McElhaney","year":"2009","journal-title":"Vaccine"},{"issue":"4","key":"10.1016\/j.vaccine.2016.08.061_b0205","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1093\/oxfordjournals.aje.a120955","article-title":"Respiratory syncytial virus disease in infants despite prior administration of antigenic inactivated vaccine","volume":"89","author":"Kim","year":"1969","journal-title":"Am J Epidemiol"},{"issue":"12","key":"10.1016\/j.vaccine.2016.08.061_b0210","doi-asserted-by":"crossref","first-page":"1591","DOI":"10.2165\/11206860-000000000-00000","article-title":"Live attenuated influenza vaccine (FluMist(R); Fluenz): a review of its use in the prevention of seasonal influenza in children and adults","volume":"71","author":"Carter","year":"2011","journal-title":"Drugs"},{"issue":"1","key":"10.1016\/j.vaccine.2016.08.061_b0215","doi-asserted-by":"crossref","first-page":"76","DOI":"10.4161\/hv.8.1.18809","article-title":"Live attenuated intranasal influenza vaccine","volume":"8","author":"Esposito","year":"2012","journal-title":"Hum Vaccin Immunother"},{"issue":"31","key":"10.1016\/j.vaccine.2016.08.061_b0220","doi-asserted-by":"crossref","first-page":"4947","DOI":"10.1016\/j.vaccine.2011.04.113","article-title":"A postmarketing evaluation of the frequency of use and safety of live attenuated influenza vaccine use in nonrecommended children younger than 5 years","volume":"29","author":"Tennis","year":"2011","journal-title":"Vaccine"},{"issue":"200","key":"10.1016\/j.vaccine.2016.08.061_b0225","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.3006366","article-title":"Vaccine-induced anti-HA2 antibodies promote virus fusion and enhance influenza virus respiratory disease","volume":"5","author":"Khurana","year":"2013","journal-title":"Sci Transl Med"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X16307575?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X16307575?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T01:57:31Z","timestamp":1692496651000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X16307575"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9]]},"references-count":45,"journal-issue":{"issue":"42","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["S0264410X16307575"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2016.08.061","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2016,9]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"M2SR, a novel live single replication influenza virus vaccine, provides effective heterosubtypic protection in mice","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2016.08.061","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2016 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}