{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T06:26:51Z","timestamp":1789885611391,"version":"4.0.1"},"reference-count":40,"publisher":"Elsevier BV","issue":"16","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/100013262","name":"State Key Laboratory of Respiratory Disease","doi-asserted-by":"crossref","award":["2014SKRD-001"],"award-info":[{"award-number":["2014SKRD-001"]}],"id":[{"id":"10.13039\/100013262","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Municipal Healthcare Joint-Innovation Major Project of Guangzhou","award":["201604020011"],"award-info":[{"award-number":["201604020011"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["RO1 AI091322"],"award-info":[{"award-number":["RO1 AI091322"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["PO1 AI060699"],"award-info":[{"award-number":["PO1 AI060699"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Science and Technology Pillar Program","award":["2013BAD12B04"],"award-info":[{"award-number":["2013BAD12B04"]}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2017,4]]},"DOI":"10.1016\/j.vaccine.2017.02.063","type":"journal-article","created":{"date-parts":[[2017,3,14]],"date-time":"2017-03-14T14:01:10Z","timestamp":1489500070000},"page":"2069-2075","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":51,"title":["DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice"],"prefix":"10.1016","volume":"35","author":[{"given":"Hang","family":"Chi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuexing","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiwen","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hualei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Gai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley","family":"Perlman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songtao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jincun","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianzhu","family":"Xia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"19","key":"10.1016\/j.vaccine.2017.02.063_b0005","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1056\/NEJMoa1211721","article-title":"Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia","volume":"367","author":"Zaki","year":"2012","journal-title":"N Engl J Med"},{"issue":"14","key":"10.1016\/j.vaccine.2017.02.063_b0010","doi-asserted-by":"crossref","first-page":"7790","DOI":"10.1128\/JVI.01244-13","article-title":"Middle East respiratory syndrome coronavirus (MERS-CoV): announcement of the coronavirus study group","volume":"87","author":"de Groot","year":"2013","journal-title":"J Virol"},{"issue":"5","key":"10.1016\/j.vaccine.2017.02.063_b0015","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1056\/NEJMoa1306742","article-title":"Hospital outbreak of Middle East respiratory syndrome coronavirus","volume":"369","author":"Assiri","year":"2013","journal-title":"N Engl J Med"},{"key":"10.1016\/j.vaccine.2017.02.063_b0020","doi-asserted-by":"crossref","first-page":"e2015033","DOI":"10.4178\/epih\/e2015033","article-title":"2015 MERS outbreak in Korea: hospital-to-hospital transmission","volume":"37","author":"Ki","year":"2015","journal-title":"Epidemiol Health"},{"key":"10.1016\/j.vaccine.2017.02.063_b0025","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.coviro.2016.01.011","article-title":"Middle East respiratory syndrome and severe acute respiratory syndrome","volume":"16","author":"Vijay","year":"2016","journal-title":"Curr Opin Virol"},{"issue":"21","key":"10.1016\/j.vaccine.2017.02.063_b0030","doi-asserted-by":"crossref","first-page":"11950","DOI":"10.1128\/JVI.01672-13","article-title":"Middle East respiratory syndrome coronavirus spike protein delivered by modified vaccinia virus Ankara efficiently induces virus-neutralizing antibodies","volume":"87","author":"Song","year":"2013","journal-title":"J Virol"},{"issue":"16","key":"10.1016\/j.vaccine.2017.02.063_b0035","doi-asserted-by":"crossref","first-page":"8651","DOI":"10.1128\/JVI.00614-15","article-title":"Protective efficacy of recombinant modified vaccinia virus Ankara delivering Middle East respiratory syndrome coronavirus spike glycoprotein","volume":"89","author":"Volz","year":"2015","journal-title":"J Virol"},{"issue":"45","key":"10.1016\/j.vaccine.2017.02.063_b0040","doi-asserted-by":"crossref","first-page":"5975","DOI":"10.1016\/j.vaccine.2014.08.058","article-title":"Immunogenicity of an adenoviral-based Middle East respiratory syndrome coronavirus vaccine in BALB\/c mice","volume":"32","author":"Kim","year":"2014","journal-title":"Vaccine"},{"issue":"4","key":"10.1016\/j.vaccine.2017.02.063_b0045","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1111\/imm.12462","article-title":"Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus","volume":"145","author":"Guo","year":"2015","journal-title":"Immunology"},{"issue":"22","key":"10.1016\/j.vaccine.2017.02.063_b0050","doi-asserted-by":"crossref","first-page":"11654","DOI":"10.1128\/JVI.01815-15","article-title":"A highly immunogenic and protective Middle East respiratory syndrome coronavirus vaccine based on a recombinant measles virus vaccine platform","volume":"89","author":"Malczyk","year":"2015","journal-title":"J Virol"},{"issue":"6268","key":"10.1016\/j.vaccine.2017.02.063_b0055","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1126\/science.aad1283","article-title":"An orthopoxvirus-based vaccine reduces virus excretion after MERS-CoV infection in dromedary camels","volume":"351","author":"Haagmans","year":"2016","journal-title":"Science"},{"issue":"46","key":"10.1016\/j.vaccine.2017.02.063_b0060","doi-asserted-by":"crossref","first-page":"6170","DOI":"10.1016\/j.vaccine.2014.08.086","article-title":"Searching for an ideal vaccine candidate among different MERS coronavirus receptor-binding fragments\u2013the importance of immunofocusing in subunit vaccine design","volume":"32","author":"Ma","year":"2014","journal-title":"Vaccine"},{"issue":"18","key":"10.1016\/j.vaccine.2017.02.063_b0065","doi-asserted-by":"crossref","first-page":"2100","DOI":"10.1016\/j.vaccine.2014.02.004","article-title":"Intranasal vaccination with recombinant receptor-binding domain of MERS-CoV spike protein induces much stronger local mucosal immune responses than subcutaneous immunization: implication for designing novel mucosal MERS vaccines","volume":"32","author":"Ma","year":"2014","journal-title":"Vaccine"},{"issue":"11","key":"10.1016\/j.vaccine.2017.02.063_b0070","doi-asserted-by":"crossref","first-page":"e112602","DOI":"10.1371\/journal.pone.0112602","article-title":"Tailoring subunit vaccine immunity with adjuvant combinations and delivery routes using the Middle East respiratory coronavirus (MERS-CoV) receptor-binding domain as an antigen","volume":"9","author":"Lan","year":"2014","journal-title":"PLoS ONE"},{"issue":"2","key":"10.1016\/j.vaccine.2017.02.063_b0075","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1038\/cmi.2015.03","article-title":"Identification of an ideal adjuvant for receptor-binding domain-based subunit vaccines against Middle East respiratory syndrome coronavirus","volume":"13","author":"Zhang","year":"2016","journal-title":"Cell Mol Immunol"},{"issue":"10","key":"10.1016\/j.vaccine.2017.02.063_b0080","doi-asserted-by":"crossref","first-page":"1438","DOI":"10.1016\/j.ebiom.2015.08.031","article-title":"Recombinant receptor binding domain protein induces partial protective immunity in rhesus macaques against Middle East respiratory syndrome coronavirus challenge","volume":"2","author":"Lan","year":"2015","journal-title":"EBioMedicine"},{"issue":"5","key":"10.1016\/j.vaccine.2017.02.063_b0085","doi-asserted-by":"crossref","first-page":"1244","DOI":"10.1080\/21645515.2015.1021527","article-title":"Optimization of antigen dose for a receptor-binding domain-based subunit vaccine against MERS coronavirus","volume":"11","author":"Tang","year":"2015","journal-title":"Hum Vaccin Immunother"},{"key":"10.1016\/j.vaccine.2017.02.063_b0090","doi-asserted-by":"crossref","first-page":"3169","DOI":"10.1016\/j.vaccine.2014.04.016","article-title":"Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice","volume":"32","author":"Coleman","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2017.02.063_b0095","article-title":"MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular immunity in rhesus macaques","author":"Wang","year":"2016","journal-title":"Oncotarget"},{"issue":"301","key":"10.1016\/j.vaccine.2017.02.063_b0100","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.aac7462","article-title":"A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates","volume":"7","author":"Muthumani","year":"2015","journal-title":"Sci Transl Med"},{"key":"10.1016\/j.vaccine.2017.02.063_b0105","doi-asserted-by":"crossref","first-page":"7712","DOI":"10.1038\/ncomms8712","article-title":"Evaluation of candidate vaccine approaches for MERS-CoV","volume":"6","author":"Wang","year":"2015","journal-title":"Nat Commun"},{"issue":"5","key":"10.1016\/j.vaccine.2017.02.063_b0110","doi-asserted-by":"crossref","DOI":"10.1128\/mBio.00650-13","article-title":"Engineering a replication-competent, propagation-defective Middle East respiratory syndrome coronavirus as a vaccine candidate","volume":"4","author":"Almaz\u00e1n","year":"2013","journal-title":"Mbio"},{"issue":"4","key":"10.1016\/j.vaccine.2017.02.063_b0115","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1046\/j.1365-2796.2003.01140.x","article-title":"DNA vaccines: a review","volume":"253","author":"Liu","year":"2003","journal-title":"J Intern Med"},{"issue":"50","key":"10.1016\/j.vaccine.2017.02.063_b0120","doi-asserted-by":"crossref","first-page":"6338","DOI":"10.1016\/j.vaccine.2008.09.026","article-title":"A SARS DNA vaccine induces neutralizing antibody and cellular immune responses in healthy adults in a Phase I clinical trial","volume":"26","author":"Martin","year":"2008","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.vaccine.2017.02.063_b0125","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1128\/CVI.00261-08","article-title":"Humoral and cellular immune responses induced by 3a DNA vaccines against severe acute respiratory syndrome (SARS) or SARS-like coronavirus in mice","volume":"16","author":"Lu","year":"2009","journal-title":"Clin Vaccine Immunol"},{"issue":"3","key":"10.1016\/j.vaccine.2017.02.063_b0130","first-page":"197","article-title":"Middle East respiratory syndrome coronavirus \u201cMERS-CoV\u201d: current knowledge gaps","volume":"16","author":"Banik","year":"2015","journal-title":"Paediatr Respir Rev"},{"issue":"9","key":"10.1016\/j.vaccine.2017.02.063_b0135","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1586\/14760584.2016.1167603","article-title":"Vaccines for the prevention against the threat of MERS-CoV","volume":"15","author":"Du","year":"2016","journal-title":"Expert Rev Vaccines"},{"issue":"6","key":"10.1016\/j.vaccine.2017.02.063_b0140","doi-asserted-by":"crossref","first-page":"761","DOI":"10.1586\/14760584.2014.912134","article-title":"Current advancements and potential strategies in the development of MERS-CoV vaccines","volume":"13","author":"Zhang","year":"2014","journal-title":"Expert Rev Vaccines"},{"issue":"7440","key":"10.1016\/j.vaccine.2017.02.063_b0145","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1038\/nature12005","article-title":"Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC","volume":"495","author":"Raj","year":"2013","journal-title":"Nature"},{"issue":"24","key":"10.1016\/j.vaccine.2017.02.063_b0150","doi-asserted-by":"crossref","first-page":"13134","DOI":"10.1128\/JVI.02433-13","article-title":"Structure of the fusion core and inhibition of fusion by a heptad-repeat peptide derived from the S protein of MERS-CoV","volume":"87","author":"Gao","year":"2013","journal-title":"J Virol"},{"issue":"3","key":"10.1016\/j.vaccine.2017.02.063_b0155","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1038\/nrmicro2090","article-title":"The spike protein of SARS-CoV \u2013a target for vaccine and therapeutic development","volume":"7","author":"Du","year":"2009","journal-title":"Nat Rev Microbiol"},{"issue":"11","key":"10.1016\/j.vaccine.2017.02.063_b0160","doi-asserted-by":"crossref","first-page":"1647","DOI":"10.1517\/14712598.2015.1092518","article-title":"Middle East respiratory syndrome: current status and future prospects for vaccine development","volume":"15","author":"Du","year":"2015","journal-title":"Expert Opin Biol Ther"},{"issue":"13","key":"10.1016\/j.vaccine.2017.02.063_b0165","doi-asserted-by":"crossref","first-page":"4970","DOI":"10.1073\/pnas.1323279111","article-title":"Rapid generation of a mouse model for Middle East respiratory syndrome","volume":"111","author":"Zhao","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"4","key":"10.1016\/j.vaccine.2017.02.063_b0170","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.immuni.2007.02.011","article-title":"The humoral immune response is initiated in lymph nodes by B Cells that acquire soluble antigen directly in the follicles","volume":"26","author":"Pape","year":"2007","journal-title":"Immunity"},{"issue":"24","key":"10.1016\/j.vaccine.2017.02.063_b0175","doi-asserted-by":"crossref","first-page":"4989","DOI":"10.1182\/blood-2009-06-229567","article-title":"B-cell follicle development remodels the conduit system and allows soluble antigen delivery to follicular dendritic cells","volume":"114","author":"Baj\u00e9noff","year":"2009","journal-title":"Blood"},{"issue":"10","key":"10.1016\/j.vaccine.2017.02.063_b0180","doi-asserted-by":"crossref","first-page":"3161","DOI":"10.1002\/(SICI)1521-4141(199810)28:10<3161::AID-IMMU3161>3.0.CO;2-Q","article-title":"Regulation of T helper cell differentiation in vivo by soluble and membrane proteins provided by antigen-presenting cells","volume":"28","author":"De Becker","year":"1998","journal-title":"Eur J Immunol"},{"issue":"1","key":"10.1016\/j.vaccine.2017.02.063_b0185","first-page":"129","article-title":"Differing effects of monoclonal anti-hapten antibodies on humoral responses to soluble or particulate antigens","volume":"52","author":"Enriquez-Rincon","year":"1984","journal-title":"Immunology"},{"issue":"6982","key":"10.1016\/j.vaccine.2017.02.063_b0190","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1038\/nature02463","article-title":"A DNA vaccine induces SARS coronavirus neutralization and protective immunity in mice","volume":"428","author":"Yang","year":"2004","journal-title":"Nature"},{"issue":"7","key":"10.1016\/j.vaccine.2017.02.063_b0195","doi-asserted-by":"crossref","first-page":"3627","DOI":"10.1128\/JVI.02988-15","article-title":"Extensive positive selection drives the evolution of nonstructural proteins in lineage C Betacoronaviruses","volume":"90","author":"Forni","year":"2016","journal-title":"J Virol"},{"issue":"1","key":"10.1016\/j.vaccine.2017.02.063_b0200","doi-asserted-by":"crossref","first-page":"100","DOI":"10.3201\/eid2201.151055","article-title":"Variations in spike glycoprotein gene of MERS-CoV, South Korea, 2015","volume":"22","author":"Kim","year":"2016","journal-title":"Emerg Infect Dis"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X17302888?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X17302888?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T17:58:16Z","timestamp":1589738296000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X17302888"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4]]},"references-count":40,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["S0264410X17302888"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2017.02.063","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2017,4]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2017.02.063","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2017 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}