{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:58:24Z","timestamp":1792695504134,"version":"4.5.0"},"reference-count":37,"publisher":"Elsevier BV","issue":"19","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2018,5]]},"DOI":"10.1016\/j.vaccine.2018.03.059","type":"journal-article","created":{"date-parts":[[2018,4,6]],"date-time":"2018-04-06T14:21:45Z","timestamp":1523024505000},"page":"2643-2649","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":16,"title":["Antibody profiling using a recombinant protein\u2013based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model"],"prefix":"10.1016","volume":"36","author":[{"given":"Tomomasa","family":"Matsuyama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natsumi","family":"Sano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomokazu","family":"Takano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takamitsu","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Motoshige","family":"Yasuike","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atushi","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiko","family":"Kawato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Kurita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunori","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukinori","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chihaya","family":"Nakayasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2018.03.059_b0005","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1073\/pnas.0408782102","article-title":"Profiling the humoral immune response to infection by using proteome microarrays: high-throughput vaccine and diagnostic antigen discovery","volume":"102","author":"Davies","year":"2005","journal-title":"Proc Nat Acad Sci United States of America"},{"key":"10.1016\/j.vaccine.2018.03.059_b0010","doi-asserted-by":"crossref","first-page":"1771","DOI":"10.1128\/CVI.00300-08","article-title":"Candidate antigens for Q fever serodiagnosis revealed by immunoscreening of a Coxiella burnetii protein microarray","volume":"15","author":"Beare","year":"2008","journal-title":"Clin Vac Immunol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0015","doi-asserted-by":"crossref","first-page":"9969","DOI":"10.1073\/pnas.1101756108","article-title":"Approach to discover T-and B-cell antigens of intracellular pathogens applied to the design of Chlamydia trachomatis vaccines","volume":"108","author":"Finco","year":"2011","journal-title":"Proc Nat Acad Sci"},{"key":"10.1016\/j.vaccine.2018.03.059_b0020","series-title":"Virus taxonomy: 8th Rep Int Comm Taxonomy Viruses","first-page":"163","author":"Chinchar","year":"2005"},{"key":"10.1016\/j.vaccine.2018.03.059_b0025","first-page":"521","volume":"4","author":"Kurita","year":"2012","journal-title":"Megalocy Vir"},{"key":"10.1016\/j.vaccine.2018.03.059_b0030","doi-asserted-by":"crossref","first-page":"e1005074","DOI":"10.1371\/journal.ppat.1005074","article-title":"A novel virus causes scale drop disease in Lates calcarifer","volume":"11","author":"de Groof","year":"2015","journal-title":"PLoS Pathogen"},{"issue":"32","key":"10.1016\/j.vaccine.2018.03.059_b0035","doi-asserted-by":"crossref","first-page":"205","DOI":"10.3147\/jsfp.32.205","article-title":"Vaccination against red sea bream iridoviral disease in red sea bream","volume":"1997","author":"Nakajima","year":"1997","journal-title":"Fish Pathol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0040","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.vetmic.2012.10.026","article-title":"Efficacy of a formalin-killed cell vaccine against infectious spleen and kidney necrosis virus (ISKNV) and immunoproteomic analysis of its major immunogenic proteins","volume":"162","author":"Dong","year":"2013","journal-title":"Veter Microbiol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0045","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1007\/s11802-012-1852-7","article-title":"Development of an inactivated iridovirus vaccine against turbot viral reddish body syndrome","volume":"11","author":"Fan","year":"2012","journal-title":"J Ocean Univ China (English Edition)"},{"key":"10.1016\/j.vaccine.2018.03.059_b0050","doi-asserted-by":"crossref","first-page":"32","DOI":"10.3147\/jsfp.51.32","article-title":"Passive immunization against red sea bream iridoviral disease in five marine fish species","volume":"51","author":"Matsuyama","year":"2016","journal-title":"Fish Pathol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0055","doi-asserted-by":"crossref","first-page":"762","DOI":"10.3181\/00379727-108-27059","article-title":"Studies with cells from marine fish in tissue culture","volume":"108","author":"Clem","year":"1961","journal-title":"Proc Soc Exper Biol Med"},{"key":"10.1016\/j.vaccine.2018.03.059_b0060","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1093\/oxfordjournals.aje.a118408","article-title":"A simple method of estimating fifty per cent endpoints 1 2","volume":"27","author":"Reed","year":"1938","journal-title":"Am J Epidemiol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0065","doi-asserted-by":"crossref","first-page":"73","DOI":"10.3147\/jsfp.45.73","article-title":"Antibody detection against red sea bream iridovirus (RSIV) in yellowtail Seriola quinqueradiata using ELISA","volume":"45","author":"Kwon","year":"2010","journal-title":"Fish Pathol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0070","doi-asserted-by":"crossref","first-page":"47","DOI":"10.3147\/jsfp.30.47","article-title":"Production of monoclonal antibodies against red sea bream iridovirus","volume":"30","author":"Nakajima","year":"1995","journal-title":"Fish Pathol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0075","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3147\/jsfp.30.115","article-title":"Immunofluorescence test for the rapid diagnosis of red sea bream iridovirus infection using monoclonal antibody","volume":"30","author":"Nakajima","year":"1995","journal-title":"Fish Pathol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0080","doi-asserted-by":"crossref","first-page":"W451","DOI":"10.1093\/nar\/gki487","article-title":"GeneMark: web software for gene finding in prokaryotes, eukaryotes and viruses","volume":"33","author":"Besemer","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"10.1016\/j.vaccine.2018.03.059_b0085","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/j.virol.2012.12.017","article-title":"Virions proteins of an RSIV-type megalocytivirus from spotted knifejaw Oplegnathus punctatus (SKIV-ZJ07)","volume":"437","author":"Shuang","year":"2013","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2018.03.059_b0090","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1016\/j.fsi.2011.08.009","article-title":"Antigenic identification of virion structural proteins from infectious spleen and kidney necrosis virus","volume":"31","author":"Xiong","year":"2011","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0095","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1038\/nmeth.1273","article-title":"Human protein factory for converting the transcriptome into an in vitro-expressed proteome","volume":"5","author":"Goshima","year":"2008","journal-title":"Nature Methods"},{"key":"10.1016\/j.vaccine.2018.03.059_b0100","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.fsi.2005.10.012","article-title":"Genetic vaccines protect red seabream, Pagrus major, upon challenge with red seabream iridovirus (RSIV)","volume":"21","author":"Caipang","year":"2006","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0105","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1111\/j.1365-2761.2007.00853.x","article-title":"Vaccination of rock bream, Oplegnathus fasciatus (Temminck & Schlegel), using a recombinant major capsid protein of fish iridovirus","volume":"31","author":"Kim","year":"2008","journal-title":"J Fish Dis"},{"key":"10.1016\/j.vaccine.2018.03.059_b0110","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1348-0421.2010.00204.x","article-title":"Protection of red sea bream Pagrus major against red sea bream iridovirus infection by vaccination with a recombinant viral protein","volume":"54","author":"Shimmoto","year":"2010","journal-title":"Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0115","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.vetimm.2012.05.021","article-title":"Selection and identification of Singapore grouper iridovirus vaccine candidate antigens using bioinformatics and DNA vaccination","volume":"149","author":"Ou-yang","year":"2012","journal-title":"Veterin Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0120","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.1128\/JVI.79.4.2010-2023.2005","article-title":"Complete genome sequence of the grouper iridovirus and comparison of genomic organization with those of other iridoviruses","volume":"79","author":"Tsai","year":"2005","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0125","doi-asserted-by":"crossref","first-page":"2236","DOI":"10.1002\/pmic.200900820","article-title":"Proteomic analysis of Singapore grouper iridovirus envelope proteins and characterization of a novel envelope protein VP088","volume":"11","author":"Zhou","year":"2011","journal-title":"Proteomics"},{"key":"10.1016\/j.vaccine.2018.03.059_b0130","doi-asserted-by":"crossref","first-page":"215","DOI":"10.3354\/dao02834","article-title":"Monoclonal antibody against a putative myristoylated membrane protein encoded by grouper iridovirus 59L gene","volume":"113","author":"Chen","year":"2015","journal-title":"Dis Aquatic Organ"},{"key":"10.1016\/j.vaccine.2018.03.059_b0135","doi-asserted-by":"crossref","first-page":"448","DOI":"10.1016\/j.virol.2010.06.034","article-title":"Frog virus 3 ORF 53R, a putative myristoylated membrane protein, is essential for virus replication in vitro","volume":"405","author":"Whitley","year":"2010","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2018.03.059_b0140","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1006\/viro.1996.0209","article-title":"Myristylation of the large surface protein is required for hepatitis B virusin vitro infectivity","volume":"218","author":"Bruss","year":"1996","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2018.03.059_b0145","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/S0092-8674(02)00612-8","article-title":"Structure of the reovirus membranepenetration protein, Mu1, in a complex with its protector protein, Sigma3","volume":"108","author":"Liemann","year":"2002","journal-title":"Cell"},{"key":"10.1016\/j.vaccine.2018.03.059_b0150","article-title":"Singapore grouper iridovirus protein VP088 is essential for viral infectivity","volume":"6","author":"Yuan","year":"2016","journal-title":"Sci Rep"},{"key":"10.1016\/j.vaccine.2018.03.059_b0155","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1099\/0022-1317-67-3-591","article-title":"Neutralizing (54K) and non-neutralizing (54K and 48K) monoclonal antibodies against structural and non-structural yellow fever virus proteins confer immunity in mice","volume":"67","author":"Gould","year":"1986","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0160","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1099\/0022-1317-68-3-853","article-title":"Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1","volume":"68","author":"Schlesinger","year":"1987","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0165","doi-asserted-by":"crossref","first-page":"192","DOI":"10.1016\/j.antiviral.2013.03.008","article-title":"The flavivirus NS1 protein: molecular and structural biology, immunology, role inpathogenesis and application asadiagnostic biomarker","volume":"98","author":"Muller","year":"2013","journal-title":"Anti Res"},{"key":"10.1016\/j.vaccine.2018.03.059_b0170","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1099\/0022-1317-83-2-369","article-title":"Hepatitis C virus non-structural protein 3-specific cellular immune responses following single or combined immunization with DNA or recombinant Semliki Forest virus particles","volume":"83","author":"Brinster","year":"2002","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0175","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2016.00040","article-title":"The non-structural protein 5 and matrix protein are antigenic targets of T cell immunity to genotype 1 porcine reproductive and respiratory syndrome viruses","volume":"7","author":"Mokhtar","year":"2016","journal-title":"Front Immunol"},{"key":"10.1016\/j.vaccine.2018.03.059_b0180","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0070197","article-title":"Ns1 is a key protein in the vaccine composition to protect ifnar(-\/-) mice against infection with multiple serotypes of african horse sickness virus","volume":"8","author":"de la Poza","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.vaccine.2018.03.059_b0185","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1016\/j.vaccine.2013.11.030","article-title":"Potential for a live red seabream iridovirus (RSIV) vaccine in rock bream Oplegnathus fasciatus at a low rearing temperature","volume":"32","author":"Oh","year":"2014","journal-title":"Vaccine"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X18304158?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X18304158?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T17:22:53Z","timestamp":1534958573000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X18304158"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5]]},"references-count":37,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["S0264410X18304158"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.03.059","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Antibody profiling using a recombinant protein\u2013based multiplex ELISA array accelerates recombinant vaccine development: Case study on red sea bream iridovirus as a reverse vaccinology model","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.03.059","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}