{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:17:16Z","timestamp":1786997836994,"version":"build-2736575974"},"reference-count":58,"publisher":"Elsevier BV","issue":"23","license":[{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2018,5,1]],"date-time":"2018-05-01T00:00:00Z","timestamp":1525132800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"name":"National Key R&D Program of China","award":["2016YFE0112500"],"award-info":[{"award-number":["2016YFE0112500"]}]},{"name":"National Key R&D Program of China","award":["2017YFD0501104"],"award-info":[{"award-number":["2017YFD0501104"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31670158"],"award-info":[{"award-number":["31670158"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Sci-Tech Support Plan Program","award":["2015BAD12B01-1"],"award-info":[{"award-number":["2015BAD12B01-1"]}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program","doi-asserted-by":"crossref","award":["2014CB542701"],"award-info":[{"award-number":["2014CB542701"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"name":"EU Horizon 2020 Program Project","award":["633184"],"award-info":[{"award-number":["633184"]}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2018,5]]},"DOI":"10.1016\/j.vaccine.2018.04.079","type":"journal-article","created":{"date-parts":[[2018,4,30]],"date-time":"2018-04-30T13:37:58Z","timestamp":1525095478000},"page":"3269-3277","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":46,"title":["Porcine reproductive and respiratory syndrome virus expressing E2 of classical swine fever virus protects pigs from a lethal challenge of highly-pathogenic PRRSV and CSFV"],"prefix":"10.1016","volume":"36","author":[{"given":"Fei","family":"Gao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifeng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanjun","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingxue","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liwei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujiao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongling","family":"Shan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangzhi","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2018.04.079_b0005","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1128\/JVI.72.1.380-387.1998","article-title":"Infectious transcripts from cloned genome-length cDNA of porcine reproductive and respiratory syndrome virus","volume":"72","author":"Meulenberg","year":"1998","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0010","first-page":"11","article-title":"PRRSV, the virus","volume":"31","author":"Meulenberg","year":"2000","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0015","doi-asserted-by":"crossref","first-page":"e526","DOI":"10.1371\/journal.pone.0000526","article-title":"Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark","volume":"2","author":"Tian","year":"2007","journal-title":"PLoS One"},{"key":"10.1016\/j.vaccine.2018.04.079_b0020","doi-asserted-by":"crossref","first-page":"2075","DOI":"10.1099\/vir.0.2008\/001529-0","article-title":"An infectious cDNA clone of a highly pathogenic porcine reproductive and respiratory syndrome virus variant associated with porcine high fever syndrome","volume":"89","author":"Lv","year":"2008","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0025","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1111\/j.1865-1682.2008.01020.x","article-title":"Highly virulent porcine reproductive and respiratory syndrome virus emerged in China","volume":"55","author":"Zhou","year":"2008","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.vaccine.2018.04.079_b0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.vetmic.2017.02.020","article-title":"Pathogenesis and control of the Chinese highly pathogenic porcine reproductive and respiratory syndrome virus","author":"Han","year":"2017","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0035","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1016\/j.virusres.2012.07.030","article-title":"Genomic sequence and virulence comparison of four Type 2 porcine reproductive and respiratory syndrome virus strains","volume":"169","author":"Brockmeier","year":"2012","journal-title":"Virus Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0040","doi-asserted-by":"crossref","first-page":"474","DOI":"10.1111\/tbed.12530","article-title":"Outbreak Investigation of NADC30-Like PRRSV in South-East China","volume":"63","author":"Li","year":"2016","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.vaccine.2018.04.079_b0045","doi-asserted-by":"crossref","first-page":"2256","DOI":"10.3201\/eid2112.150360","article-title":"NADC30-like strain of porcine reproductive and respiratory syndrome virus, China","volume":"21","author":"Zhou","year":"2015","journal-title":"Emerg Infect Dis"},{"key":"10.1016\/j.vaccine.2018.04.079_b0050","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.vetmic.2016.11.010","article-title":"Pathogenicity and antigenicity of a novel NADC30-like strain of porcine reproductive and respiratory syndrome virus emerged in China","volume":"197","author":"Zhang","year":"2016","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0055","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.meegid.2016.12.021","article-title":"Widespread of NADC30-like PRRSV in China: another Pandora's box for Chinese pig industry as the outbreak of highly pathogenic PRRSV in 2006?","volume":"49","author":"Li","year":"2017","journal-title":"Infect Genet Evol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0060","doi-asserted-by":"crossref","first-page":"59","DOI":"10.2174\/1874357901711010059","article-title":"NADC30-like porcine reproductive and respiratory syndrome in China","volume":"11","author":"Tian","year":"2017","journal-title":"Open Virol J"},{"key":"10.1016\/j.vaccine.2018.04.079_b0065","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1016\/j.vetmic.2015.06.015","article-title":"Characterization of three porcine reproductive and respiratory syndrome virus isolates from a single swine farm bearing strong homology to a vaccine strain","volume":"179","author":"Jiang","year":"2015","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0070","doi-asserted-by":"crossref","first-page":"48","DOI":"10.2174\/1874357901711010048","article-title":"Emergence and reemergence of porcine reproductive and respiratory syndrome virus (PRRSV)","volume":"11","author":"Tian","year":"2017","journal-title":"Open Virol J"},{"key":"10.1016\/j.vaccine.2018.04.079_b0075","doi-asserted-by":"crossref","first-page":"2607","DOI":"10.1099\/0022-1317-82-11-2607","article-title":"Viable porcine arteriviruses with deletions proximal to the 3\u2032 end of the genome","volume":"82","author":"Verheije","year":"2001","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0080","first-page":"774","article-title":"[Identification of porcine reproductive and respiratory syndrome virus regulation sequence in 3\u2032-untranslated region]","volume":"47","author":"Sun","year":"2007","journal-title":"Wei sheng wu xue bao\u202f=\u202fActa microbiologica Sinica"},{"key":"10.1016\/j.vaccine.2018.04.079_b0085","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1016\/j.virusres.2010.08.027","article-title":"Identification of dispensable nucleotide sequence in 3\u2032 untranslated region of porcine reproductive and respiratory syndrome virus","volume":"154","author":"Sun","year":"2010","journal-title":"Virus Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0090","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/978-1-4615-1899-0_79","article-title":"Coronaviruses use discontinuous extension for synthesis of subgenome-length negative strands","volume":"380","author":"Sawicki","year":"1995","journal-title":"Adv Exp Med Biol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0095","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.vetmic.2016.10.001","article-title":"Efficacy of the marker vaccine rAdV-SFV-E2 against classical swine fever in the presence of maternally derived antibodies to rAdV-SFV-E2 or C-strain","volume":"196","author":"Xia","year":"2016","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0100","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2014.04.004","article-title":"Classical swine fever in China: a minireview","volume":"172","author":"Luo","year":"2014","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0105","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.virusres.2014.08.012","article-title":"Host miR-26a suppresses replication of porcine reproductive and respiratory syndrome virus by upregulating type I interferons","volume":"195","author":"Li","year":"2015","journal-title":"Virus Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0110","doi-asserted-by":"crossref","first-page":"5524","DOI":"10.1016\/j.vaccine.2006.11.043","article-title":"Diagnostic methods for detection of Classical swine fever virus\u2013status quo and new developments","volume":"25","author":"Greiser-Wilke","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.04.079_b0115","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1099\/vir.0.81611-0","article-title":"Nidovirus transcription: how to make sense...?","volume":"87","author":"Pasternak","year":"2006","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0120","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1016\/S0264-410X(00)00334-0","article-title":"DNA-mediated protection against classical swine fever virus","volume":"19","author":"Yu","year":"2001","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.04.079_b0125","first-page":"151","article-title":"Sampling variation of the fifty percent end-point, determined by the Reed-Muench (Behrens) method","volume":"22","author":"Pizzi","year":"1950","journal-title":"Hum Biol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0130","doi-asserted-by":"crossref","first-page":"93","DOI":"10.4161\/auto.26843","article-title":"Autophagy enhances the replication of classical swine fever virus in vitro","volume":"10","author":"Pei","year":"2014","journal-title":"Autophagy"},{"key":"10.1016\/j.vaccine.2018.04.079_b0135","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.virusres.2009.11.014","article-title":"Recombinant PRRSV expressing porcine circovirus sequence reveals novel aspect of transcriptional control of porcine arterivirus","volume":"148","author":"Zheng","year":"2010","journal-title":"Virus Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0140","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1186\/1743-422X-8-172","article-title":"A 5\u2032-proximal stem-loop structure of 5\u2032 untranslated region of porcine reproductive and respiratory syndrome virus genome is key for virus replication","volume":"8","author":"Lu","year":"2011","journal-title":"Virol J"},{"key":"10.1016\/j.vaccine.2018.04.079_b0145","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.virol.2008.09.013","article-title":"Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus","volume":"383","author":"Yu","year":"2009","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2018.04.079_b0150","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virol.2012.12.013","article-title":"Replacement of the heterologous 5\u2032 untranslated region allows preservation of the fully functional activities of type 2 porcine reproductive and respiratory syndrome virus","volume":"439","author":"Gao","year":"2013","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2018.04.079_b0155","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.virusres.2011.08.018","article-title":"Cis-acting structural element in 5\u2032 UTR is essential for infectivity of porcine reproductive and respiratory syndrome virus","volume":"163","author":"Gao","year":"2012","journal-title":"Virus Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0160","doi-asserted-by":"crossref","first-page":"17010","DOI":"10.1038\/srep17010","article-title":"Cellular miR-130b inhibits replication of porcine reproductive and respiratory syndrome virus in vitro and in vivo","volume":"5","author":"Li","year":"2015","journal-title":"Sci Rep"},{"key":"10.1016\/j.vaccine.2018.04.079_b0165","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0378-1135(00)00195-4","article-title":"Analysis of classical swine fever virus replication kinetics allows differentiation of highly virulent from avirulent strains","volume":"74","author":"Mittelholzer","year":"2000","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0170","doi-asserted-by":"crossref","first-page":"e1004216","DOI":"10.1371\/journal.ppat.1004216","article-title":"Nsp9 and Nsp10 contribute to the fatal virulence of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China","volume":"10","author":"Li","year":"2014","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.vaccine.2018.04.079_b0175","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1177\/030098589503200606","article-title":"Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus","volume":"32","author":"Halbur","year":"1995","journal-title":"Vet Pathol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0180","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1354\/vp.37-2-143","article-title":"Pathogenesis of porcine reproductive and respiratory syndrome virus-induced increase in susceptibility to Streptococcus suis infection","volume":"37","author":"Thanawongnuwech","year":"2000","journal-title":"Vet Pathol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0185","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.biologicals.2014.12.004","article-title":"Pre-registration efficacy study of a novel marker vaccine against classical swine fever on Maternally Derived Antibody negative (MDA-) target animals","volume":"43","author":"Levai","year":"2015","journal-title":"Biologicals"},{"key":"10.1016\/j.vaccine.2018.04.079_b0190","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1016\/j.jviromet.2006.05.020","article-title":"A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses","volume":"136","author":"Hoffmann","year":"2006","journal-title":"J Virol Methods"},{"key":"10.1016\/j.vaccine.2018.04.079_b0195","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1016\/j.dci.2014.07.024","article-title":"Molecular cloning and characterizations of porcine SAMHD1 and its roles in replication of highly pathogenic porcine reproductive and respiratory syndrome virus","volume":"47","author":"Yang","year":"2014","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0200","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1186\/1743-422X-8-410","article-title":"Generation of an infectious clone of HuN4-F112, an attenuated live vaccine strain of porcine reproductive and respiratory syndrome virus","volume":"8","author":"Zhang","year":"2011","journal-title":"Virol J"},{"key":"10.1016\/j.vaccine.2018.04.079_b0205","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1006\/viro.2000.0525","article-title":"Expression of a foreign epitope by porcine reproductive and respiratory syndrome virus","volume":"278","author":"Groot Bramel-Verheije","year":"2000","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2018.04.079_b0210","doi-asserted-by":"crossref","first-page":"3086","DOI":"10.1099\/vir.0.2008\/003426-0","article-title":"Development of genetic markers in the non-structural protein 2 region of a US type 1 porcine reproductive and respiratory syndrome virus: implications for future recombinant marker vaccine development","volume":"89","author":"Fang","year":"2008","journal-title":"J Gen Virol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0215","doi-asserted-by":"crossref","first-page":"11447","DOI":"10.1128\/JVI.01032-06","article-title":"A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: expression of green fluorescent protein in the Nsp2 region","volume":"80","author":"Fang","year":"2006","journal-title":"J Virol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0220","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1186\/s13567-017-0445-2","article-title":"Role of transcription regulatory sequence in regulation of gene expression and replication of porcine reproductive and respiratory syndrome virus","volume":"48","author":"Wang","year":"2017","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0225","doi-asserted-by":"crossref","first-page":"12056","DOI":"10.1073\/pnas.96.21.12056","article-title":"Arterivirus discontinuous mRNA transcription is guided by base pairing between sense and antisense transcription-regulating sequences","volume":"96","author":"van Marle","year":"1999","journal-title":"Proc Natl Acad Sci USA"},{"key":"10.1016\/j.vaccine.2018.04.079_b0230","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/j.virol.2009.03.036","article-title":"Porcine reproductive and respiratory syndrome virus as a vector: immunogenicity of green fluorescent protein and porcine circovirus type 2 capsid expressed from dedicated subgenomic RNAs","volume":"389","author":"Pei","year":"2009","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2018.04.079_b0235","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1186\/s12985-014-0201-4","article-title":"Construction and in vitro evaluation of a recombinant live attenuated PRRSV expressing GM-CSF","volume":"11","author":"Yu","year":"2014","journal-title":"Virol J"},{"key":"10.1016\/j.vaccine.2018.04.079_b0240","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/1297-9716-44-104","article-title":"A novel porcine reproductive and respiratory syndrome virus vector system that stably expresses enhanced green fluorescent protein as a separate transcription unit","volume":"44","author":"Wang","year":"2013","journal-title":"Vet Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0245","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.virusres.2004.02.041","article-title":"Phylogenetic analysis of the E2 gene of classical swine fever viruses from Lao PDR","volume":"104","author":"Blacksell","year":"2004","journal-title":"Virus Res"},{"key":"10.1016\/j.vaccine.2018.04.079_b0250","first-page":"17","article-title":"Study on using NSP2 protein of porcine reproductive and respiratory syndrome virus (HuN4-F112) to express E2 neutralizing epitope of classical swine fever virus","volume":"29","author":"Xu","year":"2013","journal-title":"Bing Du Xue Bao"},{"key":"10.1016\/j.vaccine.2018.04.079_b0255","doi-asserted-by":"crossref","first-page":"378","DOI":"10.1186\/1743-422X-7-378","article-title":"Antigenic analysis of classical swine fever virus E2 glycoprotein using pig antibodies identifies residues contributing to antigenic variation of the vaccine C-strain and group 2 strains circulating in China","volume":"7","author":"Chen","year":"2010","journal-title":"Virol J"},{"key":"10.1016\/j.vaccine.2018.04.079_b0260","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.virol.2007.02.025","article-title":"Mutations in the carboxyl terminal region of E2 glycoprotein of classical swine fever virus are responsible for viral attenuation in swine","volume":"364","author":"Risatti","year":"2007","journal-title":"Virology"},{"key":"10.1016\/j.vaccine.2018.04.079_b0265","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1016\/j.vetmic.2003.09.008","article-title":"Vaccinology of classical swine fever: from lab to field","volume":"96","author":"van Oirschot","year":"2003","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0270","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1186\/1743-422X-9-141","article-title":"Identification of nonessential regions of the nsp2 protein of an attenuated vaccine strain (HuN4-F112) of highly pathogenic porcine reproductive and respiratory syndrome virus for replication in marc-145 cell","volume":"9","author":"Xu","year":"2012","journal-title":"Virol J"},{"key":"10.1016\/j.vaccine.2018.04.079_b0275","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.vetmic.2012.03.015","article-title":"Stable expression of foreign gene in nonessential region of nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome virus: applications for marker vaccine design","volume":"159","author":"Xu","year":"2012","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0280","doi-asserted-by":"crossref","first-page":"34","DOI":"10.1016\/j.vetmic.2009.03.003","article-title":"An attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) protects piglets against HP-PRRS","volume":"138","author":"Tian","year":"2009","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.04.079_b0285","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1007\/s11262-012-0812-z","article-title":"Use of reverse genetics to develop a novel marker porcine reproductive and respiratory syndrome virus","volume":"45","author":"Lin","year":"2012","journal-title":"Virus Genes"},{"key":"10.1016\/j.vaccine.2018.04.079_b0290","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.vaccine.2006.07.033","article-title":"Marker vaccine strategies and candidate CSFV marker vaccines","volume":"25","author":"Dong","year":"2007","journal-title":"Vaccine"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X18305863?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X18305863?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:28:34Z","timestamp":1759400914000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X18305863"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5]]},"references-count":58,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["S0264410X18305863"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.04.079","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2018,5]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Porcine reproductive and respiratory syndrome virus expressing E2 of classical swine fever virus protects pigs from a lethal challenge of highly-pathogenic PRRSV and CSFV","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.04.079","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}