{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T17:44:54Z","timestamp":1787939094868,"version":"build-2784847793"},"reference-count":51,"publisher":"Elsevier BV","issue":"5","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Chinese National Natural Science Foundation","doi-asserted-by":"crossref","award":["81301399"],"award-info":[{"award-number":["81301399"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science & Technology Department of Sichuan Province","award":["2017SZ0122"],"award-info":[{"award-number":["2017SZ0122"]}]},{"name":"Science & Technology Department of Sichuan Province","award":["2016SZ0021"],"award-info":[{"award-number":["2016SZ0021"]}]}],"content-domain":{"domain":["clinicalkey.jp","clinicalkey.com","clinicalkey.es","clinicalkey.fr","clinicalkey.com.au","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2019,1]]},"DOI":"10.1016\/j.vaccine.2018.12.021","type":"journal-article","created":{"date-parts":[[2019,1,2]],"date-time":"2019-01-02T22:18:07Z","timestamp":1546467487000},"page":"721-731","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":14,"title":["Molecular characterization of Streptococcus pneumoniae in children living in southwest China and assessment of a potential protein vaccine, rPfbA"],"prefix":"10.1016","volume":"37","author":[{"given":"Ziyi","family":"Yan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yali","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wensheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Tan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongmei","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2018.12.021_b0005","doi-asserted-by":"crossref","first-page":"77S","DOI":"10.1016\/S0002-9343(99)00104-7","article-title":"Therapy for pneumococcal infection at the millennium: doubts and certainties","volume":"107","author":"Ball","year":"1999","journal-title":"Am. J. Medi."},{"key":"10.1016\/j.vaccine.2018.12.021_b0010","doi-asserted-by":"crossref","first-page":"4717","DOI":"10.1016\/j.vaccine.2012.04.093","article-title":"Pneumococcal vaccines WHO position paper-2012-recommendations","volume":"30","author":"WHO","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.021_b0015","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1007\/s10096-016-2582-3","article-title":"Molecular characteristics of penicillin-binding protein 2b, 2x and 1a sequences in Streptococcus pneumoniae isolates causing invasive diseases among children in Northeast China","volume":"35","author":"Zhou","year":"2016","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0020","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1586\/erv.10.145","article-title":"Global prevailing and emerging pediatric pneumococcal serotypes","volume":"10","author":"McIntosh","year":"2011","journal-title":"Exp Rev Vacc"},{"key":"10.1016\/j.vaccine.2018.12.021_b0025","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1186\/s12879-015-1147-x","article-title":"Pneumococcal serotype evolution in Western Europe","volume":"15","author":"Htar","year":"2015","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0030","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/S1473-3099(16)30466-2","article-title":"Invasive pneumococcal disease in children aged younger than 5 years in India: a surveillance study","volume":"17","author":"Manoharan","year":"2017","journal-title":"Lancet Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0035","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1186\/s12879-017-2816-8","article-title":"Prevalence and serotype distribution of nasopharyngeal carriage of Streptococcus pneumoniae in China: a meta-analysis","volume":"17","author":"Wang","year":"2017","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0040","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0067190","article-title":"Structure of the Streptococcus pneumoniae Surface Protein and Adhesin PfbA","volume":"8","author":"Suits","year":"2013","journal-title":"PloS one"},{"key":"10.1016\/j.vaccine.2018.12.021_b0045","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/s00769-002-0537-0","article-title":"College of American pathologists laboratory accreditation checklists","volume":"7","author":"Rabinovitch","year":"2002","journal-title":"Accred Qual Assur"},{"key":"10.1016\/j.vaccine.2018.12.021_b0050","doi-asserted-by":"crossref","first-page":"424","DOI":"10.3345\/kjp.2012.55.11.424","article-title":"Enhanced detection and serotyping of Streptococcus pneumoniae using multiplex polymerase chain reaction","volume":"55","author":"Ahn","year":"2012","journal-title":"Kor J Pediatr"},{"key":"10.1016\/j.vaccine.2018.12.021_b0055","doi-asserted-by":"crossref","first-page":"2296","DOI":"10.3760\/cma.j.issn.0366-6999.20122925","article-title":"Serological and molecular capsular typing, antibiotic susceptibility and multilocus sequence typing of Streptococcus pneumoniae isolates from invasive and non-invasive infections","volume":"126","author":"Zhang","year":"2013","journal-title":"Chin Med J"},{"key":"10.1016\/j.vaccine.2018.12.021_b0060","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pgen.0020031","article-title":"Genetic analysis of the capsular biosynthetic locus from all 90 pneumococcal serotypes","volume":"2","author":"Bentley","year":"2006","journal-title":"PLoS Genetics"},{"key":"10.1016\/j.vaccine.2018.12.021_b0065","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/j.cimid.2014.10.005","article-title":"Genotypic characterization of Malaysian human isolates of Streptococcus pneumoniae from carriage and clinical sources","volume":"37","author":"Shakrin","year":"2014","journal-title":"Comp Immunol Microbiol Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0070","doi-asserted-by":"crossref","first-page":"3049","DOI":"10.1099\/00221287-144-11-3049","article-title":"A multilocus sequence typing scheme for Streptococcus pneumoniae: identification of clones associated with serious invasive disease","volume":"144","author":"Enright","year":"1998","journal-title":"Microbiology"},{"key":"10.1016\/j.vaccine.2018.12.021_b0075","unstructured":"Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Six Informational Supplement M100S. CLSI 2016; Wayne(PA)"},{"key":"10.1016\/j.vaccine.2018.12.021_b0080","doi-asserted-by":"crossref","first-page":"4996","DOI":"10.1016\/j.vaccine.2007.04.069","article-title":"Enhanced protection against pneumococcal infection elicited by immunization with the combination of PspA, PspC, and ClpP","volume":"25","author":"Cao","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.021_b0085","doi-asserted-by":"crossref","first-page":"1736","DOI":"10.1016\/j.vaccine.2010.12.126","article-title":"Immunization with DnaJ (hsp40) could elicit protection against nasopharyngeal colonization and invasive infection caused by different strains of Streptococcus pneumoniae","volume":"29","author":"Yali","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.021_b0090","doi-asserted-by":"crossref","first-page":"1276","DOI":"10.1128\/IAI.00473-09","article-title":"Immunization with a combination of three pneumococcal proteins confers additive and broad protection against Streptococcus pneumoniae infections in mice","volume":"78","author":"Wu","year":"2010","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2018.12.021_b0095","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1128\/CVI.00400-07","article-title":"Optimized immune response elicited by a DNA vaccine expressing pneumococcal surface protein a is characterized by a balanced immunoglobulin G1 (IgG1)\/IgG2a ratio and proinflammatory cytokine production","volume":"15","author":"Ferreira","year":"2008","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.vaccine.2018.12.021_b0100","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1128\/CVI.00395-08","article-title":"Characterization of protective mucosal and systemic immune responses elicited by pneumococcal surface protein PspA and PspC nasal vaccines against a respiratory pneumococcal challenge in mice","volume":"16","author":"Ferreira","year":"2009","journal-title":"Clin Vacc Immunol"},{"key":"10.1016\/j.vaccine.2018.12.021_b0105","doi-asserted-by":"crossref","first-page":"1870","DOI":"10.1093\/molbev\/msw054","article-title":"MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets","volume":"33","author":"Kumar","year":"2016","journal-title":"Mol Biol Evol"},{"key":"10.1016\/j.vaccine.2018.12.021_b0110","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/1471-2105-13-87","article-title":"PHYLOViZ: phylogenetic inference and data visualization for sequence based typing methods","volume":"13","author":"Francisco","year":"2012","journal-title":"BMC Bioinf"},{"key":"10.1016\/j.vaccine.2018.12.021_b0115","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1016\/j.vaccine.2017.12.063","article-title":"Phenotypic and molecular characterization of Streptococcus pneumoniae in pre-conjugate vaccine era: a Chinese hospital-based retrospective study","volume":"36","author":"Li","year":"2018","journal-title":"Vacc"},{"key":"10.1016\/j.vaccine.2018.12.021_b0120","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0093752","article-title":"Molecular characterization and antimicrobial susceptibility of Streptococcus pneumoniae isolated from children hospitalized with respiratory infections in Suzhou China.","volume":"9","author":"Geng","year":"2014","journal-title":"PloS One"},{"key":"10.1016\/j.vaccine.2018.12.021_b0125","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1016\/j.ijantimicag.2008.05.004","article-title":"Antimicrobial susceptibility and molecular epidemiology of Streptococcus pneumoniae isolated from Shanghai, China","volume":"32","author":"Yang","year":"2008","journal-title":"Int J Antimicrob Agents"},{"issue":"29","key":"10.1016\/j.vaccine.2018.12.021_b0130","first-page":"7704","article-title":"Yang Y-h. Serotype distribution and antibiotic resistance of 140 pneumococcal isolates from pediatric patients with upper respiratory infections in Beijing","volume":"2011","author":"Zhou","year":"2010","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.021_b0135","doi-asserted-by":"crossref","first-page":"e5","DOI":"10.1016\/j.ijid.2011.09.013","article-title":"Epidemiology of community-acquired pneumonia in children of Latin America and the Caribbean: a systematic review and meta-analysis","volume":"16","author":"Gentile","year":"2012","journal-title":"Int J Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0140","doi-asserted-by":"crossref","first-page":"36272","DOI":"10.1074\/jbc.M807087200","article-title":"PfbA, a novel plasmin-and fibronectin-binding protein of Streptococcus pneumoniae contributes to fibronectin-dependent adhesion and antiphagocytosis","volume":"283","author":"Yamaguchi","year":"2008","journal-title":"J Biol Chem"},{"key":"10.1016\/j.vaccine.2018.12.021_b0145","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1111\/j.1462-5822.2010.01560.x","article-title":"Cellular interactions by LPxTG-anchored pneumococcal adhesins and their streptococcal homologues","volume":"13","author":"L\u00f6fling","year":"2011","journal-title":"Cell Microbiol"},{"key":"10.1016\/j.vaccine.2018.12.021_b0150","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1007\/s00018-008-8477-4","article-title":"Pili in Gram-negative and Gram-positive bacteria\u2014structure, assembly and their role in disease","volume":"66","author":"Proft","year":"2009","journal-title":"Cell Mol Life Sci"},{"key":"10.1016\/j.vaccine.2018.12.021_b0155","doi-asserted-by":"crossref","first-page":"14","DOI":"10.7883\/yoken.JJID.2017.236","article-title":"Predominance of Serogroup 19 CC320\/271 among penicillin-nonsusceptible Streptococcus pneumoniae isolates after introduction of the PCV7 vaccine in several regions of Japan","volume":"71","author":"Oodate","year":"2018","journal-title":"Japanese J Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0160","doi-asserted-by":"crossref","first-page":"S37","DOI":"10.1016\/j.cmi.2016.01.007","article-title":"ESCMID guideline: diagnosis and treatment of acute bacterial meningitis","volume":"22","author":"Van de Beek","year":"2016","journal-title":"Clin Microbiol Infect"},{"key":"10.1016\/j.vaccine.2018.12.021_b0165","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/j.jinf.2016.01.007","article-title":"The UK joint specialist societies guideline on the diagnosis and management of acute meningitis and meningococcal sepsis in immunocompetent adults","volume":"72","author":"McGill","year":"2016","journal-title":"J Infect"},{"key":"10.1016\/j.vaccine.2018.12.021_b0170","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/pch\/19.3.141","article-title":"Guidelines for the management of suspected and confirmed bacterial meningitis in Canadian children older than one month of age","volume":"19","author":"Le Saux","year":"2014","journal-title":"Paediatrics child health."},{"key":"10.1016\/j.vaccine.2018.12.021_b0175","doi-asserted-by":"crossref","first-page":"228","DOI":"10.4103\/ijmm.IJMM_17_124","article-title":"Increasing incidence of penicillin-and cefotaxime-resistant Streptococcus pneumoniae causing meningitis in India: Time for revision of treatment guidelines?","volume":"35","author":"Verghese","year":"2017","journal-title":"Indian J Medi Microbiol"},{"key":"10.1016\/j.vaccine.2018.12.021_b0180","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1186\/s12879-017-2880-0","article-title":"Serotype distribution and antibiotic resistance of Streptococcus pneumoniae isolates from 17 Chinese cities from 2011 to 2016","volume":"17","author":"Zhao","year":"2017","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0185","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1086\/648593","article-title":"Sustained reductions in invasive pneumococcal disease in the era of conjugate vaccine","volume":"201","author":"Pilishvili","year":"2010","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0190","first-page":"450","article-title":"Lessons learnt after the introduction of the seven valent-pneumococcal conjugate vaccine toward broader spectrum conjugate vaccines","volume":"35","author":"Falup-Pecurariu","year":"2012","journal-title":"Biom J."},{"key":"10.1016\/j.vaccine.2018.12.021_b0195","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1097\/INF.0b013e3181d7d09c","article-title":"Reduction in hospitalizations for pneumonia associated with the introduction of a pneumococcal conjugate vaccination schedule without a booster dose in Australia","volume":"29","author":"Jardine","year":"2010","journal-title":"Pediatr Infect Dis J"},{"key":"10.1016\/j.vaccine.2018.12.021_b0200","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1016\/j.jinf.2014.06.006","article-title":"Review: current and new generation pneumococcal vaccines","volume":"69","author":"Feldman","year":"2014","journal-title":"J Infect"},{"key":"10.1016\/j.vaccine.2018.12.021_b0205","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.vaccine.2016.11.046","article-title":"Trivalent pneumococcal protein vaccine protects against experimental acute otitis media caused by Streptococcus pneumoniae in an infant murine model","volume":"35","author":"Xu","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.021_b0210","doi-asserted-by":"crossref","first-page":"6292","DOI":"10.1016\/j.vaccine.2016.10.061","article-title":"In vivo screen of genetically conserved Streptococcus pneumoniae proteins for protective immunogenicity","volume":"34","author":"Anderson","year":"2016","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.021_b0215","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1093\/infdis\/jit502","article-title":"Broadly protective protein-based pneumococcal vaccine composed of pneumolysin toxoid\u2013CbpA peptide recombinant fusion protein","volume":"209","author":"Mann","year":"2013","journal-title":"J Infect Dis"},{"key":"10.1016\/j.vaccine.2018.12.021_b0220","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.4049\/jimmunol.1000831","article-title":"Secretory-IgA antibodies play an important role in the immunity to Streptococcus pneumoniae","volume":"185","author":"Fukuyama","year":"2010","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2018.12.021_b0225","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.chom.2011.01.007","article-title":"TH17-based vaccine design for prevention of Streptococcus pneumoniae colonization","volume":"9","author":"Moffitt","year":"2011","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.vaccine.2018.12.021_b0230","doi-asserted-by":"crossref","first-page":"558","DOI":"10.1038\/nm1765","article-title":"Inhibition of pulmonary antibacterial defense by interferon-\u03b3 during recovery from influenza infection","volume":"14","author":"Sun","year":"2008","journal-title":"Nat Med"},{"key":"10.1016\/j.vaccine.2018.12.021_b0235","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1000159","article-title":"Interleukin-17A mediates acquired immunity to pneumococcal colonization","volume":"4","author":"Lu","year":"2008","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.vaccine.2018.12.021_b0240","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0122529","article-title":"Scavenger receptor SREC-I mediated entry of TLR4 into lipid microdomains and triggered inflammatory cytokine release in RAW 264.7 cells upon LPS activation","volume":"10","author":"Murshid","year":"2015","journal-title":"PloS One"},{"key":"10.1016\/j.vaccine.2018.12.021_b0245","doi-asserted-by":"crossref","first-page":"3921","DOI":"10.1038\/emboj.2009.360","article-title":"Molecular architecture of Streptococcus pneumoniae TIGR4 pili","volume":"28","author":"Hilleringmann","year":"2009","journal-title":"EMBO J."},{"key":"10.1016\/j.vaccine.2018.12.021_b0250","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1111\/j.1365-2958.2007.05908.x","article-title":"RrgA is a pilus-associated adhesin in Streptococcus pneumoniae","volume":"66","author":"Nelson","year":"2007","journal-title":"Mol Microbiol"},{"key":"10.1016\/j.vaccine.2018.12.021_b0255","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1128\/IAI.05780-11","article-title":"RrgB321, a fusion protein of the three variants of the pneumococcal pilus backbone RrgB, is protective in vivo and elicits opsonic antibodies","volume":"80","author":"Harfouche","year":"2012","journal-title":"Infect Immun"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X1831692X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X1831692X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2024,7,13]],"date-time":"2024-07-13T19:34:31Z","timestamp":1720899271000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X1831692X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["S0264410X1831692X"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.12.021","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,1]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Molecular characterization of Streptococcus pneumoniae in children living in southwest China and assessment of a potential protein vaccine, rPfbA","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.12.021","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2018 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}