{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T10:56:12Z","timestamp":1789815372925,"version":"4.0.1"},"reference-count":35,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2020,1,24]],"date-time":"2020-01-24T00:00:00Z","timestamp":1579824000000},"content-version":"vor","delay-in-days":357,"URL":"http:\/\/www.elsevier.com\/open-access\/userlicense\/1.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"CNPq","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004263","name":"FAPERGS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Brazilian Research Funding Agencies"}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1016\/j.vaccine.2018.12.050","type":"journal-article","created":{"date-parts":[[2019,1,8]],"date-time":"2019-01-08T20:06:15Z","timestamp":1546977975000},"page":"776-782","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":34,"title":["Recombinant BCG strains expressing chimeric proteins derived from Leptospira protect hamsters against leptospirosis"],"prefix":"10.1016","volume":"37","author":[{"given":"Tha\u00eds Larr\u00e9","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Rizzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Eduardo Pouey","family":"da Cunha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Dorneles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amilton Clair Pinto","family":"Seixas Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta Gon\u00e7alves","family":"Amaral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daiane Drawanz","family":"Hartwig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Odir Ant\u00f4nio","family":"Dellagostin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2018.12.050_b0005","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.vetmic.2009.03.012","article-title":"Leptospira and leptospirosis","volume":"140","author":"Adler","year":"2010","journal-title":"Vet Microbiol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0010","doi-asserted-by":"crossref","first-page":"736","DOI":"10.1038\/nrmicro2208","article-title":"Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen","volume":"7","author":"Ko","year":"2009","journal-title":"Nat Rev Microbiol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0015","first-page":"65","article-title":"Leptospirosis in humans","volume":"387","author":"Haake","year":"2015","journal-title":"Curr Top Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0020","doi-asserted-by":"crossref","first-page":"e154","DOI":"10.1371\/journal.pntd.0000154","article-title":"Household transmission of leptospira infection in urban slum communities","volume":"2","author":"Maciel","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.vaccine.2018.12.050_b0025","doi-asserted-by":"crossref","first-page":"e228","DOI":"10.1371\/journal.pntd.0000228","article-title":"Impact of environment and social gradient on Leptospira infection in urban slums","volume":"2","author":"Reis","year":"2008","journal-title":"PLoS Negl Trop Dis"},{"issue":"Suppl 1","key":"10.1016\/j.vaccine.2018.12.050_b0030","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/tbed.12233","article-title":"Leptospirosis in cattle: a challenging scenario for the understanding of the epidemiology","volume":"61","author":"Lilenbaum","year":"2014","journal-title":"Transbound Emerg Dis"},{"key":"10.1016\/j.vaccine.2018.12.050_b0035","first-page":"99","article-title":"Animal leptospirosis","volume":"387","author":"Ellis","year":"2015","journal-title":"Curr Top Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0040","doi-asserted-by":"crossref","first-page":"e0003898","DOI":"10.1371\/journal.pntd.0003898","article-title":"Global morbidity and mortality of leptospirosis: a systematic review","volume":"9","author":"Costa","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.vaccine.2018.12.050_b0045","doi-asserted-by":"crossref","first-page":"e0004122","DOI":"10.1371\/journal.pntd.0004122","article-title":"Global burden of leptospirosis: estimated in terms of disability adjusted life years","volume":"9","author":"Torgerson","year":"2015","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.vaccine.2018.12.050_b0050","first-page":"251","article-title":"Vaccines against leptospirosis","volume":"387","author":"Adler","year":"2015","journal-title":"Curr Top Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0055","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.4161\/hv.7.11.17944","article-title":"Recombinant vaccines against leptospirosis","volume":"7","author":"Dellagostin","year":"2011","journal-title":"Hum Vaccin"},{"key":"10.1016\/j.vaccine.2018.12.050_b0060","doi-asserted-by":"crossref","first-page":"463","DOI":"10.3389\/fimmu.2017.00463","article-title":"Discovery of novel leptospirosis vaccine candidates using reverse and structural vaccinology","volume":"8","author":"Grassmann","year":"2017","journal-title":"Front Immunol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0065","first-page":"187","article-title":"The leptospiral outer membrane","volume":"387","author":"Haake","year":"2015","journal-title":"Curr Top Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0070","doi-asserted-by":"crossref","first-page":"e0005441","DOI":"10.1371\/journal.pntd.0005441","article-title":"LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis","volume":"11","author":"Conrad","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.vaccine.2018.12.050_b0075","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1128\/CVI.00034-13","article-title":"A prime-boost strategy using the novel vaccine candidate, LemA, protects hamsters against leptospirosis","volume":"20","author":"Hartwig","year":"2013","journal-title":"Clin Vaccine Immunol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0080","doi-asserted-by":"crossref","first-page":"6277","DOI":"10.1016\/j.vaccine.2007.05.053","article-title":"The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis","volume":"25","author":"Silva","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.050_b0085","doi-asserted-by":"crossref","first-page":"6495","DOI":"10.1016\/j.vaccine.2009.08.044","article-title":"Recombinant Mycobacterium bovis BCG","volume":"27","author":"Bastos","year":"2009","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.050_b0090","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.micinf.2005.03.012","article-title":"Improved protection by recombinant BCG","volume":"7","author":"Nasser Eddine","year":"2005","journal-title":"Microbes Infect"},{"key":"10.1016\/j.vaccine.2018.12.050_b0095","doi-asserted-by":"crossref","DOI":"10.1007\/s00253-017-8439-6","article-title":"Recombinant BCG vaccines: molecular features and their influence in the expression of foreign genes","author":"Oliveira","year":"2017","journal-title":"Appl Microbiol Biotechnol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0100","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1186\/1754-1611-2-5","article-title":"Engineering BioBrick vectors from BioBrick parts","volume":"2","author":"Shetty","year":"2008","journal-title":"J Biol Eng"},{"key":"10.1016\/j.vaccine.2018.12.050_b0105","first-page":"237","article-title":"Electroporation of mycobacteria","volume":"47","author":"Parish","year":"1995","journal-title":"Methods Mol Biol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0110","doi-asserted-by":"crossref","first-page":"e1422","DOI":"10.1371\/journal.pntd.0001422","article-title":"A LigA three-domain region protects hamsters from lethal infection by Leptospira interrogans","volume":"5","author":"Coutinho","year":"2011","journal-title":"PLoS Negl Trop Dis"},{"key":"10.1016\/j.vaccine.2018.12.050_b0115","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0165-2427(00)00198-7","article-title":"Nucleic acid vaccines: research tool or commercial reality","volume":"76","author":"Babiuk","year":"2000","journal-title":"Vet Immunol Immunopathol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0120","doi-asserted-by":"crossref","first-page":"995","DOI":"10.2460\/ajvr.2001.62.995","article-title":"Use of a monovalent leptospiral vaccine to prevent renal colonization and urinary shedding in cattle exposed to Leptospira borgpetersenii serovar hardjo","volume":"62","author":"Bolin","year":"2001","journal-title":"Am J Vet Res"},{"key":"10.1016\/j.vaccine.2018.12.050_b0125","doi-asserted-by":"crossref","first-page":"7550","DOI":"10.1128\/IAI.69.12.7550-7558.2001","article-title":"Protective killed Leptospira borgpetersenii vaccine induces potent Th1 immunity comprising responses by CD4 and gammadelta T lymphocytes","volume":"69","author":"Naiman","year":"2001","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2018.12.050_b0130","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0165-2478(98)00128-X","article-title":"Impact of intracellular location of and antigen display by intracellular bacteria: implications for vaccine development","volume":"65","author":"Kaufmann","year":"1999","journal-title":"Immunol Lett"},{"key":"10.1016\/j.vaccine.2018.12.050_b0135","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1099\/00221287-148-7-1999","article-title":"Comparative evaluation of Mycobacterium vaccae as a surrogate cloning host for use in the study of mycobacterial genetics","volume":"148","author":"Medeiros","year":"2002","journal-title":"Microbiology"},{"key":"10.1016\/j.vaccine.2018.12.050_b0140","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.vaccine.2007.10.052","article-title":"Recombinant Mycobacterium bovis BCG expressing the LipL32 antigen of Leptospira interrogans protects hamsters from challenge","volume":"26","author":"Seixas","year":"2007","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.050_b0145","doi-asserted-by":"crossref","first-page":"904","DOI":"10.1016\/S0264-410X(98)00276-X","article-title":"Safety and immunogenicity of recombinant Bacille Calmette-Guerin (rBCG) expressing Borrelia burgdorferi outer surface protein A (OspA) lipoprotein in adult volunteers: a candidate Lyme disease vaccine","volume":"17","author":"Edelman","year":"1999","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.050_b0150","first-page":"223","article-title":"Host response to leptospira infection","volume":"387","author":"Zuerner","year":"2015","journal-title":"Curr Top Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0155","first-page":"21","article-title":"Leptospiral structure, physiology, and metabolism","volume":"387","author":"Cameron","year":"2015","journal-title":"Curr Top Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0160","doi-asserted-by":"crossref","first-page":"e32712","DOI":"10.1371\/journal.pone.0032712","article-title":"Detection and quantification of Leptospira interrogans in hamster and rat kidney samples: immunofluorescent imprints versus real-time PCR","volume":"7","author":"Chagas-Junior","year":"2012","journal-title":"PLoS ONE"},{"key":"10.1016\/j.vaccine.2018.12.050_b0165","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/j.ijid.2017.01.032","article-title":"Immune response and protective profile elicited by a multi-epitope chimeric protein derived from Leptospira interrogans","volume":"57","author":"Fernandes","year":"2017","journal-title":"Int J Infect Dis"},{"issue":"1","key":"10.1016\/j.vaccine.2018.12.050_b0170","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1186\/s12866-016-0852-y","article-title":"Chimeric epitope vaccine against Leptospira interrogans infection and induced specific immunity in guinea pigs","volume":"16","author":"Lin","year":"2016","journal-title":"BMC Microbiol"},{"key":"10.1016\/j.vaccine.2018.12.050_b0175","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.micpath.2018.08.028","article-title":"Antigenic potential of a recombinant polyvalent DNA vaccine against pathogenic leptospiral infection","volume":"124","author":"Garba","year":"2018","journal-title":"Microb Pathog"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X19300064?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X19300064?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:29:37Z","timestamp":1759400977000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X19300064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":35,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["S0264410X19300064"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.12.050","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Recombinant BCG strains expressing chimeric proteins derived from Leptospira protect hamsters against leptospirosis","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.12.050","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd.","name":"copyright","label":"Copyright"}]}}