{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T17:34:05Z","timestamp":1782495245183,"version":"3.54.5"},"reference-count":45,"publisher":"Elsevier BV","issue":"7","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003566","name":"Ministry of Oceans and Fisheries","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003566","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2019,2]]},"DOI":"10.1016\/j.vaccine.2018.12.063","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T22:25:21Z","timestamp":1547763921000},"page":"973-983","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":68,"title":["PLGA encapsulated inactivated-viral vaccine: Formulation and evaluation of its protective efficacy against viral haemorrhagic septicaemia virus (VHSV) infection in olive flounder (Paralichthys olivaceus) vaccinated by mucosal delivery routes"],"prefix":"10.1016","volume":"37","author":[{"given":"Sajal","family":"Kole","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Syed Shariq Nazir","family":"Qadiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Mi","family":"Shin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wi-Sik","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8851-6121","authenticated-orcid":false,"given":"Jehee","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung-Ju","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2018.12.063_b0005","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.ympev.2011.12.024","article-title":"Evolution and biogeography of an emerging quasispecies: diversity patterns of the fish Viral Hemorrhagic Septicemia virus (VHSv)","volume":"63","author":"Pierce","year":"2012","journal-title":"Mol Phylogenet Evol"},{"issue":"1\u20132","key":"10.1016\/j.vaccine.2018.12.063_b0010","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.aquaculture.2009.07.019","article-title":"An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed olive flounder Paralichthys olivaceus in Korea","volume":"296","author":"Kim","year":"2009","journal-title":"Aquaculture"},{"key":"10.1016\/j.vaccine.2018.12.063_b0015","doi-asserted-by":"crossref","first-page":"87","DOI":"10.3354\/dao047087","article-title":"An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed Japanese flounder Paralichthys olivaceus in Japan","volume":"47","author":"Isshiki","year":"2001","journal-title":"Dis Aquat Org"},{"issue":"1","key":"10.1016\/j.vaccine.2018.12.063_b0020","doi-asserted-by":"crossref","first-page":"201","DOI":"10.20506\/rst.24.1.1565","article-title":"DNA vaccines for aquacultured fish","volume":"24","author":"Lorenzen","year":"2005","journal-title":"Rev Sci Tech Int Epiz"},{"issue":"41","key":"10.1016\/j.vaccine.2018.12.063_b0025","doi-asserted-by":"crossref","first-page":"4603","DOI":"10.1016\/j.vaccine.2013.07.036","article-title":"Inactivated vaccine against viral hemorrhagic septicemia (VHS) emulsified with squalene and aluminium hydroxide adjuvant provides long term protection in olive flounder (Paralichthys olivaceus)","volume":"31","author":"Vinay","year":"2013","journal-title":"Vaccine"},{"issue":"1","key":"10.1016\/j.vaccine.2018.12.063_b0030","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/S0169-409X(00)00075-2","article-title":"Application of DNA vaccine technology to aquaculture","volume":"43","author":"Heppell","year":"2000","journal-title":"Adv Drug Deliv Rev"},{"issue":"2","key":"10.1016\/j.vaccine.2018.12.063_b0035","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1016\/j.fsi.2009.11.009","article-title":"Parenteral immunization of fish, Labeo rohita with Poly D,L-lactide-co-glycolic acid (PLGA) encapsulated antigen microparticles promotes innate and adaptive immune responses","volume":"28","author":"Behera","year":"2010","journal-title":"Fish Shellfish Immunol"},{"issue":"23","key":"10.1016\/j.vaccine.2018.12.063_b0040","doi-asserted-by":"crossref","first-page":"2475","DOI":"10.1016\/S0142-9612(00)00115-0","article-title":"The manufacturing techniques of various drug loaded biodegradable poly(lactide-co-glycolide) (PLGA) devices","volume":"21","author":"Jain","year":"2000","journal-title":"Biomaterials"},{"key":"10.1016\/j.vaccine.2018.12.063_b0045","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/12_2011_150","article-title":"Biodegradable nanoparticles as vaccine adjuvants and delivery systems: regulation of immune responses by nanoparticle based vaccine","volume":"247","author":"Akagi","year":"2012","journal-title":"Adv Polym Sci"},{"key":"10.1016\/j.vaccine.2018.12.063_b0050","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1016\/j.intimp.2008.02.006","article-title":"The formulation and immunisation of oral poly(DL-lactide-coglycolide) microcapsules containing a plasmid vaccine against lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)","volume":"8","author":"Tian","year":"2008","journal-title":"Int Immunopharmacol"},{"issue":"46","key":"10.1016\/j.vaccine.2018.12.063_b0055","doi-asserted-by":"crossref","first-page":"8338","DOI":"10.1016\/j.vaccine.2011.08.087","article-title":"Early immune responses in Atlantic salmon (Salmo salar L.) after immunization with PLGA nanoparticles loaded with a model antigen and beta-glucan","volume":"29","author":"Fredriksen","year":"2011","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.063_b0060","doi-asserted-by":"crossref","first-page":"1070","DOI":"10.1016\/S0264-410X(97)00013-3","article-title":"Oral immunization of rainbow trout with antigen microencapsulated in poly(DL-lactide-co-glycolide) microparticles","volume":"15","author":"Lavelle","year":"1997","journal-title":"Vaccine"},{"issue":"3","key":"10.1016\/j.vaccine.2018.12.063_b0065","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1111\/j.1365-2761.2011.01338.x","article-title":"Oral DNA vaccination of rainbow trout, Oncorhynchus mykiss (Walbaum), against infectious haematopoietic necrosis virus using PLGA [Poly(D,L-Lactic-Co-Glycolic Acid)] nanoparticles","volume":"35","author":"Adomako","year":"2012","journal-title":"J Fish Dis"},{"key":"10.1016\/j.vaccine.2018.12.063_b0070","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.exppara.2012.04.017","article-title":"Poly D,L-lactide-co-glycolic acid (PLGA)-encapsulated vaccine on immune system in Epinephelus bruneus against Uronema marinum","volume":"131","author":"Harikrishnan","year":"2012","journal-title":"Exp Parasitol"},{"issue":"27","key":"10.1016\/j.vaccine.2018.12.063_b0075","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/j.vaccine.2012.04.039","article-title":"Comparison of vaccine efficacy for different antigen delivery systems for infectious pancreatic necrosis virus vaccines in Atlantic salmon (Salmo salar L.) in a cohabitation challenge model","volume":"30","author":"Munang'andu","year":"2012","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.063_b0080","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3354\/dao034013","article-title":"Rapid and sensitive reverse transcriptase-polymerase chain reaction based detection and differential diagnosis of fish pathogenic rhabdoviruses in organ samples and cultured cells","volume":"34","author":"Miller","year":"1998","journal-title":"Dis Aquat Org"},{"key":"10.1016\/j.vaccine.2018.12.063_b0085","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.1016\/S0264-410X(99)00140-1","article-title":"PLG microparticles stabilised using enteric coating polymers as oral vaccine delivery systems","volume":"17","author":"Delgado","year":"1999","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.063_b0090","first-page":"447","article-title":"Potency testing of fish vaccines","volume":"49","author":"Amend","year":"1981","journal-title":"Dev Biol Standard"},{"key":"10.1016\/j.vaccine.2018.12.063_b0095","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.1016\/j.vaccine.2018.02.099","article-title":"Nanoconjugation of bicistronic DNA vaccine against Edwardsiella tarda using chitosan nanoparticles: evaluation of its protective efficacy and immune modulatory effects in Labeo rohita vaccinated by different delivery routes","volume":"36","author":"Kole","year":"2018","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.063_b0100","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real time quantitative PCR and the 2(-Delta Delta C(T)) method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"10.1016\/j.vaccine.2018.12.063_b0105","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/j.fsi.2011.06.009","article-title":"Toll-like receptors and interferon associated immune factors in viral haemorrhagic septicaemia virus-infected olive flounder (Paralichthys olivaceus)","volume":"31","author":"Avunje","year":"2011","journal-title":"Fish Shellfish Immunol"},{"issue":"1","key":"10.1016\/j.vaccine.2018.12.063_b0110","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0378-5173(02)00058-3","article-title":"Biocompatible stabilizers in the preparation of PLGA nanoparticles: a factorial design study","volume":"238","author":"Vandervoort","year":"2002","journal-title":"Int J Pharm"},{"key":"10.1016\/j.vaccine.2018.12.063_b0115","doi-asserted-by":"crossref","first-page":"1108","DOI":"10.1038\/sj.gt.3301492","article-title":"Chitosan as a nonviral gene delivery system. Structure-property relationships and characteristics compared with polyethyleneimine in vitro and after lung administration in vivo","volume":"8","author":"K\u00f6ping-H\u00f6gg\u00e5rd","year":"2001","journal-title":"Gene Ther"},{"issue":"15","key":"10.1016\/j.vaccine.2018.12.063_b0120","doi-asserted-by":"crossref","first-page":"2713","DOI":"10.1016\/j.biomaterials.2004.07.050","article-title":"Effects of particle size and surface coating on cellular uptake of polymeric nanoparticles for oral delivery of anticancer drugs","volume":"26","author":"Win","year":"2005","journal-title":"Biomaterials"},{"key":"10.1016\/j.vaccine.2018.12.063_b0125","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/S0169-409X(01)00171-5","article-title":"Chitosan as a novel nasal delivery system for vaccines","volume":"51","author":"Illum","year":"2001","journal-title":"Adv Drug Del Rev"},{"key":"10.1016\/j.vaccine.2018.12.063_b0130","doi-asserted-by":"crossref","first-page":"2363","DOI":"10.1007\/s11051-010-9996-0","article-title":"Preparation of surfactant-stabilized gold nanoparticle\u2013peptide nucleic acid conjugates","volume":"12","author":"Duy","year":"2010","journal-title":"J Nanoparticle Res"},{"issue":"35","key":"10.1016\/j.vaccine.2018.12.063_b0135","doi-asserted-by":"crossref","first-page":"5344","DOI":"10.1016\/j.biomaterials.2007.08.015","article-title":"Interactions of antigen-loaded polylactide particles with macrophages and their correlation with the immune response","volume":"28","author":"Kanchan","year":"2007","journal-title":"Biomaterials"},{"issue":"1","key":"10.1016\/j.vaccine.2018.12.063_b0140","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.fsi.2015.02.007","article-title":"Parenteral immunization of PLA\/PLGA nanoparticle encapsulating outer membrane protein (Omp) from Aeromonas hydrophila: evaluation of immunostimulatory action in Labeo rohita (rohu)","volume":"44","author":"Rauta","year":"2015","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0145","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1016\/j.dci.2011.11.009","article-title":"Mucosal immunoglobulins and B cells of teleost fish","volume":"35","author":"Salinas","year":"2011","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0150","doi-asserted-by":"crossref","first-page":"525","DOI":"10.3390\/biology4030525","article-title":"The mucosal immune system of teleost fish","volume":"4","author":"Salinas","year":"2015","journal-title":"Biology"},{"key":"10.1016\/j.vaccine.2018.12.063_b0155","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S1050-4648(10)80006-3","article-title":"Morphology of the gut associated lymphoid tissue of Oreochromis mossambicus and its role in antigen absorption","volume":"1","author":"Doggett","year":"1991","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0160","doi-asserted-by":"crossref","first-page":"3959","DOI":"10.1016\/j.vaccine.2017.06.005","article-title":"Efficacy of PLGA microparticle-encapsulated formalin-killed Aeromonas hydrophila cells as a single-shot vaccine against A. hydrophila infection","volume":"13","author":"Yun","year":"2017","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2018.12.063_b0165","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1577\/1548-8667(1993)005<0165:COTHRO>2.3.CO;2","article-title":"Characterization of the humoral response of rainbow trout to infectious hematopoietic necrosis virus","volume":"5","author":"Lapatra","year":"1993","journal-title":"J Aquat Anim Health"},{"key":"10.1016\/j.vaccine.2018.12.063_b0170","doi-asserted-by":"crossref","first-page":"427","DOI":"10.3389\/fimmu.2015.00427","article-title":"A review of the immunological mechanisms following mucosal vaccination of finfish","volume":"6","author":"Munang\u2019andu","year":"2015","journal-title":"Front Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0175","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.dci.2017.05.004","article-title":"Oral DNA vaccines based on CS-TPP nanoparticles and alginate microparticles confer high protection against infectious pancreatic necrosis virus (IPNV) infection in trout","volume":"74","author":"Ahmadivand","year":"2017","journal-title":"Dev Comp Immunol"},{"issue":"37","key":"10.1016\/j.vaccine.2018.12.063_b0180","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.fsi.2014.02.003","article-title":"Food pellets as an effective delivery method for a DNA vaccine against infectious pancreatic necrosis virus in rainbow trout (Oncorhynchus mykiss, Walbaum)","volume":"2014","author":"Ballesteros","year":"2014","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0185","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1016\/j.fsi.2015.05.045","article-title":"An oral DNA vaccine against infectious haematopoietic necrosis virus (IHNV) encapsulated in alginate microspheres induces dose dependent immune responses and significant protection in rainbow trout (Oncorrhynchus mykiss)","volume":"45","author":"Ballesteros","year":"2015","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0190","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.fsi.2014.08.005","article-title":"Immune gene expressions in grouper larvae (Epinephelus coioides) induced by bath and oral vaccinations with inactivated betanodavirus","volume":"40","author":"Kai","year":"2014","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0195","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1016\/j.dci.2016.03.013","article-title":"B cells and their role in the teleost gut","volume":"64","author":"Parra","year":"2016","journal-title":"Dev Comp Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0200","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s10126-010-9279-x","article-title":"Immunological control of fish diseases","volume":"12","author":"Magnadottir","year":"2010","journal-title":"Mar Biotechnol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0205","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1016\/j.fsi.2012.03.016","article-title":"Oral immunization of rainbow trout to infectious pancreatic necrosis virus (IPNV) induces different immune gene expression profiles in head kidney and pyloric ceca","volume":"33","author":"Ballesteros","year":"2012","journal-title":"Fish Shellfish Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0210","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1016\/j.fsi.2009.12.006","article-title":"Immunogenic and protective effects of an oral DNA vaccine against infectious pancreatic necrosis virus in fish","volume":"28","author":"De-Las Heras","year":"2010","journal-title":"Fish Shellfish Immunol"},{"issue":"4","key":"10.1016\/j.vaccine.2018.12.063_b0215","doi-asserted-by":"crossref","first-page":"2484","DOI":"10.4049\/jimmunol.175.4.2484","article-title":"Identification and bioactivities of IFN-\u03b3 in rainbow trout Oncorhynchus mykiss: the first Th1-type cytokine characterized functionally in fish","volume":"175","author":"Zou","year":"2005","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0220","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1038\/ni.1863","article-title":"The role of pattern-recognition receptors in innate immunity: update on toll-like receptors","volume":"11","author":"Kawai","year":"2010","journal-title":"Nat Immunol"},{"key":"10.1016\/j.vaccine.2018.12.063_b0225","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nrm2312","article-title":"Apoptosis: controlled demolition at the cellular level","volume":"9","author":"Taylor","year":"2008","journal-title":"Nat Rev Mol Cell Biol"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X19300428?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X19300428?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:29:46Z","timestamp":1759400986000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X19300428"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2]]},"references-count":45,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,2]]}},"alternative-id":["S0264410X19300428"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.12.063","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2019,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"PLGA encapsulated inactivated-viral vaccine: Formulation and evaluation of its protective efficacy against viral haemorrhagic septicaemia virus (VHSV) infection in olive flounder (Paralichthys olivaceus) vaccinated by mucosal delivery routes","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2018.12.063","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2019 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}