{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:27:58Z","timestamp":1791613678454,"version":"4.3.4"},"reference-count":47,"publisher":"Elsevier BV","issue":"6","license":[{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2021,2,1]],"date-time":"2021-02-01T00:00:00Z","timestamp":1612137600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1016\/j.vaccine.2020.12.072","type":"journal-article","created":{"date-parts":[[2021,1,13]],"date-time":"2021-01-13T14:27:18Z","timestamp":1610548038000},"page":"933-942","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":15,"title":["Synergistic effect of GRA7 and profilin proteins in vaccination against chronic Toxoplasma gondii infection"],"prefix":"10.1016","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0938-1527","authenticated-orcid":false,"given":"Nadia","family":"Arcon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano S.","family":"Picchio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio M.","family":"Fenoy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3740-0062","authenticated-orcid":false,"given":"Rosal\u00eda E.","family":"Moretta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariadna S.","family":"Soto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas D.","family":"Perrone Sibilia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6684-7947","authenticated-orcid":false,"given":"Vanesa R.","family":"S\u00e1nchez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia A.","family":"Prato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Virginia","family":"Tribulatti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Goldman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2020.12.072_b0005","doi-asserted-by":"crossref","first-page":"1385","DOI":"10.1016\/j.ijpara.2009.04.003","article-title":"Toxoplasmosis snapshots: Global status of Toxoplasma gondii seroprevalence and implications for pregnancy and congenital toxoplasmosis","volume":"39","author":"Pappas","year":"2009","journal-title":"Int J Parasitol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0010","doi-asserted-by":"crossref","DOI":"10.1128\/MMBR.00027-15","article-title":"Long-Term Relationships: the Complicated Interplay between the Host and the Developmental Stages of Toxoplasma gondii during Acute and Chronic Infections","author":"Pittman","year":"2015","journal-title":"Microbiol Mol Biol Rev"},{"key":"10.1016\/j.vaccine.2020.12.072_b0015","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s40588-016-0045-3","article-title":"Reexamining Chronic Toxoplasma gondii Infection: Surprising Activity for a \u201cDormant\u201d Parasite","volume":"3","author":"Sinai","year":"2016","journal-title":"Curr Clin Microbiol Reports"},{"key":"10.1016\/j.vaccine.2020.12.072_b0020","article-title":"Toxoplasma gondii infection in immunocompromised patients: A systematic review and meta-analysis","author":"Wang","year":"2017","journal-title":"Front Microbiol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0025","doi-asserted-by":"crossref","DOI":"10.4103\/2229-5070.190813","article-title":"Congenital toxoplasmosis: Clinical features, outcomes, treatment, and prevention","author":"Singh","year":"2016","journal-title":"Trop Parasitol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0030","doi-asserted-by":"crossref","DOI":"10.1016\/j.pt.2019.01.005","article-title":"Advances in the Development of Anti-Toxoplasma gondii Vaccines: Challenges, Opportunities, and Perspectives","author":"Wang","year":"2019","journal-title":"Trends Parasitol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0035","doi-asserted-by":"crossref","DOI":"10.1128\/CMR.00057-17","article-title":"Treatment of toxoplasmosis: Historical perspective, animal models, and current clinical practice","author":"Dunay","year":"2018","journal-title":"Clin Microbiol Rev"},{"key":"10.1016\/j.vaccine.2020.12.072_b0040","doi-asserted-by":"crossref","DOI":"10.1136\/vr.129.5.89","article-title":"Vaccination of sheep with a live incomplete strain (S48) of Toxoplasma gondii and their immunity to challenge when pregnant","author":"Buxton","year":"1991","journal-title":"Vet Rec"},{"key":"10.1016\/j.vaccine.2020.12.072_b0045","first-page":"779","article-title":"Novel adjuvants & delivery vehicles for vaccines development: A road ahead","volume":"138","author":"Mohan","year":"2013","journal-title":"Indian J Med Res"},{"key":"10.1016\/j.vaccine.2020.12.072_b0050","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1007\/s40588-019-0114-5","article-title":"Toxoplasma: Immunity and Pathogenesis","volume":"6","author":"Khan","year":"2019","journal-title":"Curr Clin Microbiol Reports"},{"key":"10.1016\/j.vaccine.2020.12.072_b0055","doi-asserted-by":"crossref","unstructured":"Rezaei F, Sarvi S, Sharif M, Hejazi SH, Pagheh A sattar, Aghayan SA, et al. A systematic review of Toxoplasma gondii antigens to find the best vaccine candidates for immunization. Microb Pathog 2019. https:\/\/doi.org\/10.1016\/j.micpath.2018.11.003.","DOI":"10.1016\/j.micpath.2018.11.003"},{"key":"10.1016\/j.vaccine.2020.12.072_b0060","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1016\/j.pt.2015.02.002","article-title":"Toxoplasma secretory granules: one population or more?","volume":"31","author":"Mercier","year":"2015","journal-title":"Trends Parasitol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0065","first-page":"165","article-title":"A systematic review on the role of GRA proteins of Toxoplasma gondii in host immunization","author":"Rezaei","year":"2019","journal-title":"J Microbiol Methods"},{"key":"10.1016\/j.vaccine.2020.12.072_b0070","first-page":"797","article-title":"The moving junction of apicomplexan parasites: A key structure for invasion","volume":"13","author":"Besteiro","year":"2011","journal-title":"CellMicrobiol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0075","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.chom.2008.01.001","article-title":"Toxoplasma Profilin Is Essential for Host Cell Invasion and TLR11-Dependent Induction of an Interleukin-12 Response","volume":"3","author":"Plattner","year":"2008","journal-title":"Cell Host Microbe"},{"key":"10.1016\/j.vaccine.2020.12.072_b0080","doi-asserted-by":"crossref","unstructured":"Yarovinsky F, Zhang D, Andersen JF, Bannenberg GL, Serhan CN, Hayden MS, et al. Immunology: TLR11 activation of dendritic cells by a protozoan profilin-like protein. Science (80-) 2005;308:1626\u20139. https:\/\/doi.org\/10.1126\/science.1109893.","DOI":"10.1126\/science.1109893"},{"key":"10.1016\/j.vaccine.2020.12.072_b0085","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.1016\/j.vaccine.2014.01.095","article-title":"Vaccination with profilin encapsulated in oligomannose-coated liposomes induces significant protective immunity against Toxoplasma gondii","volume":"32","author":"Tanaka","year":"2014","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2020.12.072_b0090","first-page":"9","article-title":"Immunization with a DNA vaccine cocktail encoding TgPF, TgROP16, TgROP18, TgMIC6, and TgCDPK3 genes protects mice against chronic toxoplasmosis","author":"Zhang","year":"2018","journal-title":"Front Immunol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0095","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12879-018-3022-z","article-title":"Immune response and protective effect against chronic Toxoplasma gondii infection induced by vaccination with a DNA vaccine encoding profilin","volume":"18","author":"Gao","year":"2018","journal-title":"BMC Infect Dis"},{"key":"10.1016\/j.vaccine.2020.12.072_b0100","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S0732-8893(03)00156-1","article-title":"Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis","volume":"47","author":"Nigro","year":"2003","journal-title":"Diagn Microbiol Infect Dis"},{"key":"10.1016\/j.vaccine.2020.12.072_b0105","doi-asserted-by":"crossref","unstructured":"Jespersen MC, Peters B, Nielsen M, Marcatili P. BepiPred-2.0: Improving sequence-based B-cell epitope prediction using conformational epitopes. Nucleic Acids Res 2017;45:W24\u20139. https:\/\/doi.org\/10.1093\/nar\/gkx346.","DOI":"10.1093\/nar\/gkx346"},{"key":"10.1016\/j.vaccine.2020.12.072_b0110","doi-asserted-by":"crossref","DOI":"10.1016\/j.isci.2020.101278","article-title":"Galectin-8 Enhances T cell Response by Promotion of Antigen Internalization and Processing","volume":"23","author":"Prato","year":"2020","journal-title":"IScience"},{"key":"10.1016\/j.vaccine.2020.12.072_b0115","first-page":"150","article-title":"Homologous prime-boost strategy with TgPI-1 improves the immune response and protects highly susceptible mice against chronic Toxoplasma gondii infection","author":"S\u00e1nchez","year":"2015","journal-title":"Acta Trop"},{"key":"10.1016\/j.vaccine.2020.12.072_b0120","first-page":"185","article-title":"Vaccine potential of antigen cocktails composed of recombinant Toxoplasma gondii TgPI-1, ROP2 and GRA4 proteins against chronic toxoplasmosis in C3H mice","author":"Picchio","year":"2018","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0125","first-page":"128","article-title":"Combination of CpG-oligodeoxynucleotides with recombinant ROP2 or GRA4 proteins induces protective immunity against Toxoplasma gondii infection","author":"S\u00e1nchez","year":"2011","journal-title":"Exp Parasitol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0130","doi-asserted-by":"crossref","first-page":"581","DOI":"10.3347\/kjp.2014.52.6.581","article-title":"Recent advances in Toxoplasma gondii immunotherapeutics","volume":"52","author":"Lim","year":"2014","journal-title":"Korean J Parasitol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0135","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.immuni.2012.09.016","article-title":"Recognition of Profilin by Toll-like Receptor 12 Is Critical for Host Resistance to Toxoplasma gondii","volume":"38","author":"Koblansky","year":"2013","journal-title":"Immunity"},{"key":"10.1016\/j.vaccine.2020.12.072_b0140","doi-asserted-by":"crossref","first-page":"8478","DOI":"10.4049\/jimmunol.181.12.8478","article-title":"Recognition of Toxoplasma gondii by TLR11 Prevents Parasite-Induced Immunopathology","volume":"181","author":"Yarovinsky","year":"2008","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0145","doi-asserted-by":"crossref","unstructured":"Criscuolo E, Caputo V, Diotti RA, Sautto GA, Kirchenbaum GA, Clementi N. Alternative methods of vaccine delivery: An overview of edible and intradermal vaccines. J Immunol Res 2019;2019. https:\/\/doi.org\/10.1155\/2019\/8303648.","DOI":"10.1155\/2019\/8303648"},{"key":"10.1016\/j.vaccine.2020.12.072_b0150","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1128\/IAI.00734-15","article-title":"Toxoplasma gondii GRA7-induced TRAF6 activation contributes to host protective immunity","volume":"84","author":"Yang","year":"2015","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2020.12.072_b0155","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1007\/s00281-012-0339-3","article-title":"Immune response and immunopathology during toxoplasmosis","volume":"34","author":"Dupont","year":"2012","journal-title":"Semin Immunopathol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0160","doi-asserted-by":"crossref","first-page":"2036","DOI":"10.4049\/jimmunol.181.3.2036","article-title":"Deletion of Flagellin\u2019s Hypervariable Region Abrogates Antibody-Mediated Neutralization and Systemic Activation of TLR5-Dependent Immunity","volume":"181","author":"Nempont","year":"2008","journal-title":"J Immunol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0165","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1007\/s00430-016-0452-3","article-title":"The antigen-specific response to Toxoplasma gondii profilin, a TLR11\/12 ligand, depends on its intrinsic adjuvant properties","volume":"205","author":"Hedhli","year":"2016","journal-title":"Med Microbiol Immunol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0170","doi-asserted-by":"crossref","first-page":"74107","DOI":"10.18632\/oncotarget.12316","article-title":"Immune adjuvant effect of a Toxoplasma gondii profilin-like protein in autologous whole-tumor-cell vaccination in mice","volume":"7","author":"Pyo","year":"2016","journal-title":"Oncotarget"},{"key":"10.1016\/j.vaccine.2020.12.072_b0175","doi-asserted-by":"crossref","unstructured":"Neal LM, Knoll LJ. Toxoplasma gondii Profilin Promotes Recruitment of Ly6Chi CCR2+ Inflammatory Monocytes That Can Confer Resistance to Bacterial Infection. PLoS Pathog 2014;10. https:\/\/doi.org\/10.1371\/journal.ppat.1004203.","DOI":"10.1371\/journal.ppat.1004203"},{"key":"10.1016\/j.vaccine.2020.12.072_b0180","doi-asserted-by":"crossref","first-page":"2169","DOI":"10.3389\/fimmu.2018.02169","article-title":"Determination of T Follicular Helper Cell Fate by Dendritic Cells","volume":"9","author":"Krishnaswamy","year":"2018","journal-title":"Front Immunol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0185","doi-asserted-by":"crossref","unstructured":"Nish SA, Schenten D, Wunderlich T, Pope SD, Gao Y, Hoshi N, et al. T cell-intrinsic role of IL-6 signaling in primary and memory responses. Elife 2014;2014. https:\/\/doi.org\/10.7554\/eLife.01949.","DOI":"10.7554\/eLife.01949.025"},{"key":"10.1016\/j.vaccine.2020.12.072_b0190","doi-asserted-by":"crossref","first-page":"2339","DOI":"10.1128\/iai.65.6.2339-2345.1997","article-title":"Impaired resistance to the development of toxoplasmic encephalitis in interleukin-6-deficient mice","volume":"65","author":"Suzuki","year":"1997","journal-title":"Infect Immun"},{"key":"10.1016\/j.vaccine.2020.12.072_b0195","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1046\/j.1365-3024.1998.00152.x","article-title":"A protective role for IL-6 during early infection with Toxoplasma gondii","volume":"20","author":"Jebbari","year":"1998","journal-title":"Parasite Immunol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0200","doi-asserted-by":"crossref","first-page":"909","DOI":"10.1007\/s12275-017-7392-5","article-title":"Dense Granule Protein-7 (GRA-7) of Toxoplasma gondii inhibits viral replication in vitro and in vivo","volume":"55","author":"Weeratunga","year":"2017","journal-title":"J Microbiol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0205","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.ppat.1006126","article-title":"Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKC\u03b1","volume":"13","author":"Koh","year":"2017","journal-title":"PLoS Pathog"},{"key":"10.1016\/j.vaccine.2020.12.072_b0210","doi-asserted-by":"crossref","unstructured":"Ihara F, Fereig RM, Himori Y, Kameyama K, Umeda K, Tanaka S, et al. Toxoplasma gondii Dense Granule Proteins 7, 14, and 15 Are Involved in Modification and Control of the Immune Response Mediated via NF-\u03baB Pathway. Front Immunol 2020;11. https:\/\/doi.org\/10.3389\/fimmu.2020.01709.","DOI":"10.3389\/fimmu.2020.01709"},{"key":"10.1016\/j.vaccine.2020.12.072_b0215","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1038\/nri.2015.10","article-title":"The multifaceted role of CD4+ T cells in CD8+ T cell memory","volume":"16","author":"Laidlaw","year":"2016","journal-title":"Nat Rev Immunol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0220","first-page":"8","article-title":"Immunization with Toxoplasma gondii GRA17 deletion mutant induces partial protection and survival in challenged mice","author":"Wang","year":"2017","journal-title":"Front Immunol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0225","first-page":"9","article-title":"Toxoplasma gondii: CD8 T cells cry for CD4 help","author":"Khan","year":"2019","journal-title":"Front Cell Infect Microbiol"},{"key":"10.1016\/j.vaccine.2020.12.072_b0230","doi-asserted-by":"crossref","unstructured":"Yang W Bin, Zhou DH, Zou Y, Chen K, Liu Q, Wang JL, et al. Vaccination with a DNA vaccine encoding Toxoplasma gondii ROP54 induces protective immunity against toxoplasmosis in mice. Acta Trop 2017;176:427\u201332. https:\/\/doi.org\/10.1016\/j.actatropica.2017.09.007.","DOI":"10.1016\/j.actatropica.2017.09.007"},{"key":"10.1016\/j.vaccine.2020.12.072_b0235","doi-asserted-by":"crossref","unstructured":"Hedhli D, Dimier-Poisson I, Judge JW, Rosenberg B, M\u00e9v\u00e9lec MN. Protective immunity against Toxoplasma challenge in mice by coadministration of T. gondii antigens and Eimeria profilin-like protein as an adjuvant. Vaccine 2009;27:2274\u201381. https:\/\/doi.org\/10.1016\/j.vaccine.2009.01.100.","DOI":"10.1016\/j.vaccine.2009.01.100"}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X20316698?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X20316698?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T10:51:17Z","timestamp":1759402277000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X20316698"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,2]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["S0264410X20316698"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2020.12.072","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2021,2]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Synergistic effect of GRA7 and profilin proteins in vaccination against chronic Toxoplasma gondii infection","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2020.12.072","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2020 Elsevier Ltd. All rights reserved.","name":"copyright","label":"Copyright"}]}}