{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T08:27:26Z","timestamp":1793608046528,"version":"4.5.0"},"reference-count":37,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T00:00:00Z","timestamp":1788912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP223fa627002"],"award-info":[{"award-number":["JP223fa627002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019670","name":"The Research Foundation for Microbial Diseases of the University of Osaka","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100019670","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP24K22020"],"award-info":[{"award-number":["JP24K22020"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23K27343"],"award-info":[{"award-number":["JP23K27343"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["clinicalkey.com","clinicalkey.com.au","clinicalkey.es","clinicalkey.fr","clinicalkey.jp","elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Vaccine"],"published-print":{"date-parts":[[2026,10]]},"DOI":"10.1016\/j.vaccine.2026.129136","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T01:20:09Z","timestamp":1789089609000},"page":"129136","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["Linker-mediated single-transcript mRNA vaccine expressing virus-like particles of enterovirus D68"],"prefix":"10.1016","volume":"92","author":[{"given":"Yuta","family":"Kunishima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kota","family":"Senpuku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kotaro","family":"Taniguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chikako","family":"Kataoka-Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiro","family":"Hirai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Yoshioka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vaccine.2026.129136_bb0005","doi-asserted-by":"crossref","first-page":"e64","DOI":"10.1016\/S1473-3099(15)00543-5","article-title":"Global emergence of enterovirus D68: a systematic review","volume":"16","author":"Holm-Hansen","year":"2016","journal-title":"Lancet Infect. Dis."},{"key":"10.1016\/j.vaccine.2026.129136_bb0010","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1001\/jama.2019.1016","article-title":"Enterovirus D68-associated acute flaccid myelitis: rising to the clinical and research challenges","volume":"321","author":"Messacar","year":"2019","journal-title":"JAMA"},{"key":"10.1016\/j.vaccine.2026.129136_bb0015","doi-asserted-by":"crossref","DOI":"10.1172\/JCI188495","article-title":"Immune cells promote paralytic disease in mice infected with enterovirus D68","volume":"135","author":"Woods Acevedo","year":"2025","journal-title":"J. Clin. Invest."},{"key":"10.1016\/j.vaccine.2026.129136_bb0020","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1038\/nrmicro906","article-title":"Polio eradication, cessation of vaccination and re-emergence of disease","volume":"2","author":"Minor","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"10.1016\/j.vaccine.2026.129136_bb0025","doi-asserted-by":"crossref","first-page":"199","DOI":"10.3390\/vaccines9030199","article-title":"Enterovirus A71 vaccines","volume":"9","author":"Li","year":"2021","journal-title":"Vaccines (Basel)"},{"key":"10.1016\/j.vaccine.2026.129136_bb0030","doi-asserted-by":"crossref","first-page":"1078","DOI":"10.1038\/s41578-021-00358-0","article-title":"Lipid nanoparticles for mRNA delivery","volume":"6","author":"Hou","year":"2021","journal-title":"Nat. Rev. Mater."},{"key":"10.1016\/j.vaccine.2026.129136_bb0035","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1038\/s41573-021-00283-5","article-title":"mRNA vaccines for infectious diseases: principles, delivery and clinical translation","volume":"20","author":"Chaudhary","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"10.1016\/j.vaccine.2026.129136_bb0040","doi-asserted-by":"crossref","DOI":"10.1016\/j.omtn.2025.102731","article-title":"mRNA vaccine expressing enterovirus D68 virus-like particles induces potent neutralizing antibodies and protects against infection","volume":"36","author":"Kunishima","year":"2025","journal-title":"Mol Ther Nucleic Acids"},{"key":"10.1016\/j.vaccine.2026.129136_bb0045","doi-asserted-by":"crossref","first-page":"eadg6076","DOI":"10.1126\/sciadv.adg6076","article-title":"EV-D68 virus-like particle vaccines elicit cross-clade neutralizing antibodies that inhibit infection and block dissemination","volume":"9","author":"Krug","year":"2023","journal-title":"Sci. Adv."},{"key":"10.1016\/j.vaccine.2026.129136_bb0050","series-title":"Seventy-fourth report","author":"WHO Expert Committee on Biological Standardization","year":"2022"},{"key":"10.1016\/j.vaccine.2026.129136_bb0055","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1038\/nbt957","article-title":"Correction of multi-gene deficiency in vivo using a single \u201cself-cleaving\u201d 2A peptide-based retroviral vector","volume":"22","author":"Szymczak","year":"2004","journal-title":"Nat. Biotechnol."},{"key":"10.1016\/j.vaccine.2026.129136_bb0060","first-page":"1","article-title":"High cleavage efficiency of a 2A peptide derived from porcine teschovirus-1 in human cell lines, zebrafish and mice","volume":"6","author":"Kim","year":"2011","journal-title":"PLoS One"},{"key":"10.1016\/j.vaccine.2026.129136_bb0065","doi-asserted-by":"crossref","DOI":"10.1128\/mbio.00358-23","article-title":"Advances and breakthroughs in IRES-directed translation and replication of picornaviruses","volume":"14","author":"Abdullah","year":"2023","journal-title":"MBio"},{"key":"10.1016\/j.vaccine.2026.129136_bb0070","doi-asserted-by":"crossref","first-page":"4339","DOI":"10.1128\/JVI.01123-12","article-title":"3C protease of enterovirus 68: structure-based design of Michael acceptor inhibitors and their broad-spectrum antiviral effects against picornaviruses","volume":"87","author":"Tan","year":"2013","journal-title":"J. Virol."},{"key":"10.1016\/j.vaccine.2026.129136_bb0075","doi-asserted-by":"crossref","DOI":"10.1002\/jmv.29947","article-title":"Seroprevalence of enterovirus D68 in Yamagata, Japan, between 1976 and 2019","volume":"96","author":"Itagaki","year":"2024","journal-title":"J. Med. Virol."},{"key":"10.1016\/j.vaccine.2026.129136_bb0080","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1007\/BF01863914","article-title":"Beitrag zur kollektiven behandlung pharmakologischer reihenversuche","volume":"162","author":"K\u00e4rber","year":"1931","journal-title":"Archiv f Experiment Pathol u Pharmakol"},{"key":"10.1016\/j.vaccine.2026.129136_bb0085","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.omtn.2019.02.018","article-title":"A facile method for the removal of dsRNA contaminant from in vitro-transcribed mRNA","volume":"15","author":"Baiersd\u00f6rfer","year":"2019","journal-title":"Mol Ther Nucleic Acids"},{"key":"10.1016\/j.vaccine.2026.129136_bb0090","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.omtn.2019.01.013","article-title":"Optimization of lipid nanoparticles for intramuscular administration of mRNA vaccines","volume":"15","author":"Hassett","year":"2019","journal-title":"Mol Ther Nucleic Acids"},{"key":"10.1016\/j.vaccine.2026.129136_bb0095","doi-asserted-by":"crossref","first-page":"6455","DOI":"10.1038\/s41467-025-61775-1","article-title":"Chemical circularization of in vitro transcribed RNA for exploring circular mRNA design","volume":"16","author":"Wasinska-Kalwa","year":"2025","journal-title":"Nat. Commun."},{"key":"10.1016\/j.vaccine.2026.129136_bb0100","doi-asserted-by":"crossref","first-page":"560","DOI":"10.1261\/rna.079982.124","article-title":"The je ne sais quoi of 5-methylcytosine in messenger RNA","volume":"30","author":"Guarnacci","year":"2024","journal-title":"RNA"},{"key":"10.1016\/j.vaccine.2026.129136_bb0105","article-title":"Protease-independent production of poliovirus virus-like particles in Pichia pastoris: implications for efficient vaccine development and insights into capsid assembly","author":"Sherry","year":"2022","journal-title":"Microbiol. Spectrum"},{"key":"10.1016\/j.vaccine.2026.129136_bb0110","doi-asserted-by":"crossref","first-page":"860","DOI":"10.1038\/s42003-025-08253-y","article-title":"Structural insights from vaccine candidates for EV-D68","volume":"8","author":"Cheng","year":"2025","journal-title":"Commun. Biol."},{"key":"10.1016\/j.vaccine.2026.129136_bb0115","article-title":"Inactivated enterovirus 71 vaccine produced by 200-L scale serum-free microcarrier bioreactor system provides cross-protective efficacy in human SCARB2 transgenic mouse","volume":"10","author":"Wu","year":"2015","journal-title":"PLoS One"},{"key":"10.1016\/j.vaccine.2026.129136_bb0120","doi-asserted-by":"crossref","first-page":"2463","DOI":"10.1016\/j.vaccine.2024.03.016","article-title":"An inactivated whole-virion vaccine for enterovirus D68 adjuvanted with CpG ODN or AddaVax elicits potent protective immunity in mice","volume":"42","author":"Senpuku","year":"2024","journal-title":"Vaccine"},{"key":"10.1016\/j.vaccine.2026.129136_bb0125","article-title":"Enterovirus D68 3C protease antagonizes type I interferon signaling by cleaving signal transducer and activator of transcription 1","author":"Li","year":"2024","journal-title":"J. Virol."},{"key":"10.1016\/j.vaccine.2026.129136_bb0130","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.xphs.2023.11.023","article-title":"Factors affecting stability of RNA - temperature, length, concentration, pH, and buffering species","volume":"113","author":"Chheda","year":"2024","journal-title":"J. Pharm. Sci."},{"key":"10.1016\/j.vaccine.2026.129136_bb0135","doi-asserted-by":"crossref","DOI":"10.1016\/j.biopha.2021.111953","article-title":"Engineering of the current nucleoside-modified mRNA-LNP vaccines against SARS-CoV-2","volume":"142","author":"Granados-Riveron","year":"2021","journal-title":"Biomed. Pharmacother."},{"key":"10.1016\/j.vaccine.2026.129136_bb0140","doi-asserted-by":"crossref","first-page":"491","DOI":"10.3201\/eid3204.251022","article-title":"Circulation patterns, genetic diversity, and public health implications of enterovirus D68, Europe, 2014-2024","volume":"32","author":"Andr\u00e9s","year":"2026","journal-title":"Emerg. Infect. Dis."},{"key":"10.1016\/j.vaccine.2026.129136_bb0145","doi-asserted-by":"crossref","first-page":"S920","DOI":"10.1093\/ofid\/ofz360.2313","article-title":"2635. Outbreak of enterovirus D68 among children in Japan and simultaneous circulation of clade B3 in Europe","volume":"6","author":"Ikuse","year":"2019","journal-title":"Open Forum Infect. Dis."},{"key":"10.1016\/j.vaccine.2026.129136_bb0150","doi-asserted-by":"crossref","DOI":"10.1093\/ofid\/ofaf695.2342","article-title":"P-2179. Successful early detection of the Enterovirus-D68 outbreak among children by wastewater surveillance in Niigata City, Japan, 2024","volume":"13","author":"Tachikawa","year":"2026","journal-title":"Open Forum Infect. Dis."},{"key":"10.1016\/j.vaccine.2026.129136_bb0155","doi-asserted-by":"crossref","first-page":"2360","DOI":"10.1016\/j.ymthe.2023.06.017","article-title":"A localizing nanocarrier formulation enables multi-target immune responses to multivalent replicating RNA with limited systemic inflammation","volume":"31","author":"Kimura","year":"2023","journal-title":"Mol. Ther."},{"key":"10.1016\/j.vaccine.2026.129136_bb0160","doi-asserted-by":"crossref","DOI":"10.1126\/scitranslmed.adi1625","article-title":"A self-amplifying RNA vaccine prevents enterovirus D68 infection and disease in preclinical models","volume":"16","author":"Warner","year":"2024","journal-title":"Sci. Transl. Med."},{"key":"10.1016\/j.vaccine.2026.129136_bb0165","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1039\/D4CB00022F","article-title":"Pseudouridine and N1-methylpseudouridine as potent nucleotide analogues for RNA therapy and vaccine development","volume":"5","author":"Ho","year":"2024","journal-title":"RSC Chem Biol"},{"key":"10.1016\/j.vaccine.2026.129136_bb0170","doi-asserted-by":"crossref","first-page":"10373","DOI":"10.1093\/nar\/gku720","article-title":"40S recruitment in the absence of eIF4G\/4A by EMCV IRES refines the model for translation initiation on the archetype of type II IRESs","volume":"42","author":"Chamond","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"10.1016\/j.vaccine.2026.129136_bb0175","doi-asserted-by":"crossref","first-page":"4977","DOI":"10.1038\/s41467-023-40582-6","article-title":"Dynamically regulated two-site interaction of viral RNA to capture host translation initiation factor","volume":"14","author":"Imai","year":"2023","journal-title":"Nat. Commun."},{"key":"10.1016\/j.vaccine.2026.129136_bb0180","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.immuni.2005.06.008","article-title":"Suppression of RNA recognition by toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA","volume":"23","author":"Karik\u00f3","year":"2005","journal-title":"Immunity"},{"key":"10.1016\/j.vaccine.2026.129136_bb0185","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1038\/mt.2008.200","article-title":"Incorporation of pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability","volume":"16","author":"Karik\u00f3","year":"2008","journal-title":"Mol. Ther."}],"container-title":["Vaccine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X2600945X?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S0264410X2600945X?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T07:39:28Z","timestamp":1793605168000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S0264410X2600945X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":37,"alternative-id":["S0264410X2600945X"],"URL":"https:\/\/doi.org\/10.1016\/j.vaccine.2026.129136","relation":{},"ISSN":["0264-410X"],"issn-type":[{"value":"0264-410X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Linker-mediated single-transcript mRNA vaccine expressing virus-like particles of enterovirus D68","name":"articletitle","label":"Article Title"},{"value":"Vaccine","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vaccine.2026.129136","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"129136"}}