{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:37:20Z","timestamp":1787506640392,"version":"build-2736575974"},"reference-count":49,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,6,1]],"date-time":"2025-06-01T00:00:00Z","timestamp":1748736000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T00:00:00Z","timestamp":1742428800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000943","name":"Commonwealth Scientific and Industrial Research Organisation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000943","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary and Animal Science"],"published-print":{"date-parts":[[2025,6]]},"DOI":"10.1016\/j.vas.2025.100445","type":"journal-article","created":{"date-parts":[[2025,3,28]],"date-time":"2025-03-28T08:14:02Z","timestamp":1743149642000},"page":"100445","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Circulating microRNA profiles are associated with acute pain and stress in castrated and tail docked lambs"],"prefix":"10.1016","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1636-1815","authenticated-orcid":false,"given":"Ryan J.","family":"Farr","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1969-9371","authenticated-orcid":false,"given":"Christopher","family":"Cowled","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4420-6401","authenticated-orcid":false,"given":"Carlos","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina L.","family":"Rootes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4028-8347","authenticated-orcid":false,"given":"Dana L.M.","family":"Campbell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1900-635X","authenticated-orcid":false,"given":"Caroline","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3395-7859","authenticated-orcid":false,"given":"Cameron R.","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1082-6848","authenticated-orcid":false,"given":"Danila","family":"Marini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vas.2025.100445_bib0001","series-title":"A quality control tool for high throughput sequence data","author":"Andrews","year":"2010"},{"key":"10.1016\/j.vas.2025.100445_bib0002","doi-asserted-by":"crossref","first-page":"289","DOI":"10.3389\/fvets.2019.00289","article-title":"An appraisal of methods for measuring welfare of grazing ruminants","volume":"6","author":"Barrell","year":"2019","journal-title":"Frontiers in Veterinary Science"},{"key":"10.1016\/j.vas.2025.100445_bib0003","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1016\/j.applanim.2014.08.004","article-title":"Investigating the motivation to play in lambs","volume":"160","author":"Chapagain","year":"2014","journal-title":"Applied Animal Behaviour Science"},{"key":"10.1016\/j.vas.2025.100445_bib0004","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1016\/j.gene.2014.04.071","article-title":"Expression differences of miRNAs and genes on NF-\u03bab pathway between the healthy and the mastitis Chinese Holstein cows","volume":"545","author":"Chen","year":"2014","journal-title":"Gene"},{"key":"10.1016\/j.vas.2025.100445_bib0005","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1093\/af\/vfx004","article-title":"Public animal welfare discussions and outlooks in Australia","volume":"8","author":"Coleman","year":"2018","journal-title":"Animal Frontiers"},{"key":"10.1016\/j.vas.2025.100445_bib0006","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.rvsc.2018.11.024","article-title":"Differential expression of miRNAs in milk exosomes of cows subjected to group relocation","volume":"122","author":"Colitti","year":"2019","journal-title":"Research in Veterinary Science"},{"key":"10.1016\/j.vas.2025.100445_bib0007","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1163\/15685306-12341601","article-title":"Australian consumers\u2019 knowledge and concern for animal welfare in food production: Influences on purchasing intentions","volume":"30","author":"Cornish","year":"2019","journal-title":"Society & Animals"},{"key":"10.1016\/j.vas.2025.100445_bib51","doi-asserted-by":"crossref","first-page":"e75","DOI":"10.1002\/vro2.75","article-title":"Improving analgesia provision for sheep: An analysis of farm medicine records and attitudes towards pain relief on sheep farms in Northern Ireland","volume":"10","author":"Crawford","year":"2023","journal-title":"Vet Record Open"},{"key":"10.1016\/j.vas.2025.100445_bib0008","doi-asserted-by":"crossref","first-page":"E82","DOI":"10.1002\/vetr.82","article-title":"Towards an improved pain assessment in castrated horses using facial expressions (HGS) and circulating miRNAs","volume":"188","author":"Dalla Costa","year":"2021","journal-title":"Veterinary Record"},{"key":"10.1016\/j.vas.2025.100445_bib0009","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1093\/bja\/85.1.109","article-title":"The stress response to trauma and surgery","volume":"85","author":"Desborough","year":"2000","journal-title":"BJA: British Journal of Anaesthesia"},{"key":"10.1016\/j.vas.2025.100445_bib0010","doi-asserted-by":"crossref","first-page":"28","DOI":"10.3390\/ani7040028","article-title":"Stakeholder perceptions of welfare issues and indicators for extensively managed sheep in Australia","volume":"7","author":"Doughty","year":"2017","journal-title":"Animals"},{"key":"10.1016\/j.vas.2025.100445_bib0011","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1009759","article-title":"Altered microRNA expression in COVID-19 patients enables identification of SARS-CoV-2 infection","volume":"17","author":"Farr","year":"2021","journal-title":"PLOS Pathogens"},{"key":"10.1016\/j.vas.2025.100445_bib0012","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/nar\/gkr688","article-title":"miRDeep2 accurately identifies known and hundreds of novel microRNA genes in seven animal clades","volume":"40","author":"Friedl\u00e4nder","year":"2012","journal-title":"Nucleic Acids Research"},{"key":"10.1016\/j.vas.2025.100445_bib0013","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.smallrumres.2010.04.018","article-title":"Diagnostic significance of behaviour changes of sheep: A selected review","volume":"92","author":"Gougoulis","year":"2010","journal-title":"Small Ruminant Research"},{"key":"10.1016\/j.vas.2025.100445_bib0014","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.applanim.2004.01.011","article-title":"Behavioural responses of lambs to common painful husbandry procedures","volume":"87","author":"Grant","year":"2004","journal-title":"Applied Animal Behaviour Science"},{"key":"10.1016\/j.vas.2025.100445_bib0015","series-title":"MicroRNA protocols","first-page":"129","article-title":"miRBase:The microRNA sequence database","author":"Griffiths-Jones","year":"2006"},{"key":"10.1016\/j.vas.2025.100445_bib0016","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1023\/A:1012487302797","article-title":"Gene selection for cancer classification using Support Vector machines","volume":"46","author":"Guyon","year":"2002","journal-title":"Machine Learning"},{"key":"10.1016\/j.vas.2025.100445_bib0017","series-title":"FASTX-Toolkit","author":"Hannon","year":"2010"},{"key":"10.1016\/j.vas.2025.100445_bib0018","doi-asserted-by":"crossref","DOI":"10.3389\/fnins.2022.887042","article-title":"The neuroimmune interface and chronic pain through the lens of production animals","volume":"16","author":"Johnston","year":"2022","journal-title":"Frontiers in Neuroscience"},{"key":"10.1016\/j.vas.2025.100445_bib0019","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1038\/nsmb.1630","article-title":"Structural determinants of miRNAs for RISC loading and slicer-independent unwinding","volume":"16","author":"Kawamata","year":"2009","journal-title":"Nature Structural & Molecular Biology"},{"key":"10.1016\/j.vas.2025.100445_bib0020","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3390\/ani9010030","article-title":"The effect of virtual fencing stimuli on stress responses and behavior in sheep","volume":"9","author":"Kearton","year":"2019","journal-title":"Animals"},{"key":"10.1016\/j.vas.2025.100445_bib0021","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast gapped-read alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nature Methods"},{"key":"10.1016\/j.vas.2025.100445_bib0022","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1017\/S1751731117001525","article-title":"Circulating miR-23b-3p, miR-145-5p and miR-200b-3p are potential biomarkers to monitor acute pain associated with laminitis in horses","volume":"12","author":"Lecchi","year":"2018","journal-title":"Animal : An International Journal of Animal Bioscience"},{"key":"10.1016\/j.vas.2025.100445_bib0023","doi-asserted-by":"crossref","first-page":"Skaa153","DOI":"10.1093\/jas\/skaa153","article-title":"Salivary microRNAs are potential biomarkers for the accurate and precise identification of inflammatory response after tail docking and castration in piglets","volume":"98","author":"Lecchi","year":"2020","journal-title":"Journal of Animal Science"},{"key":"10.1016\/j.vas.2025.100445_bib0024","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.molcel.2010.09.027","article-title":"MicroRNA functions in stress responses","volume":"40","author":"Leung","year":"2010","journal-title":"Molecular Cell"},{"key":"10.1016\/j.vas.2025.100445_bib0025","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.cell.2004.12.035","article-title":"Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets","volume":"120","author":"Lewis","year":"2005","journal-title":"Cell"},{"key":"10.1016\/j.vas.2025.100445_bib0026","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1186\/s12864-018-5298-1","article-title":"Characterization of miRNA profiles in the mammary tissue of dairy cattle in response to heat stress","volume":"19","author":"Li","year":"2018","journal-title":"BMC Genomics"},{"key":"10.1016\/j.vas.2025.100445_bib0027","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1111\/age.12096","article-title":"Identification and characterization of novel and differentially expressed microRNAs in peripheral blood from healthy and mastitis Holstein cattle by deep sequencing","volume":"45","author":"Li","year":"2014","journal-title":"Animal Genetics"},{"key":"10.1016\/j.vas.2025.100445_bib0028","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biology"},{"key":"10.1016\/j.vas.2025.100445_bib0029","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1007\/s12192-018-00965-x","article-title":"The role of N6-methyladenosine RNA methylation in the heat stress response of sheep (Ovis aries)","volume":"24","author":"Lu","year":"2019","journal-title":"Cell Stress and Chaperones"},{"key":"10.1016\/j.vas.2025.100445_bib0031","series-title":"Proteomics in domestic animals: From farm to systems biology","first-page":"339","article-title":"The use of proteomics to study biomarkers of stress and welfare in farm animals","author":"Marco-Ramell","year":"2018"},{"key":"10.1016\/j.vas.2025.100445_bib0032","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.applanim.2016.12.008","article-title":"Self-administration by consumption of flunixin in feed alleviates the pain and inflammation associated with castration and tail docking of lambs","volume":"188","author":"Marini","year":"2017","journal-title":"Applied Animal Behaviour Science"},{"key":"10.1016\/j.vas.2025.100445_bib0033","doi-asserted-by":"crossref","DOI":"10.3389\/fanim.2021.741631","article-title":"Can lambs in pain identify medicated feed?","volume":"2","author":"Marini","year":"2021","journal-title":"Frontiers in Animal Science"},{"issue":"No 1","key":"10.1016\/j.vas.2025.100445_bib0034","article-title":"Cutadapt removes adapter sequences from high-throughput sequencing reads","volume":"Vol 17","author":"Martin","year":"2011","journal-title":"EMBnetjournal"},{"key":"10.1016\/j.vas.2025.100445_bib0035","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1111\/j.1751-0813.2002.tb10820.x","article-title":"A comparison of catecholamine and cortisol responses of young lambs and calves to painful husbandry procedures","volume":"80","author":"Mellor","year":"2002","journal-title":"Australian Veterinary Journal"},{"key":"10.1016\/j.vas.2025.100445_bib0037","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2020.578193","article-title":"MicroRNAs as biomarkers for animal health and welfare in livestock","volume":"7","author":"Miretti","year":"2020","journal-title":"Frontiers in Veterinary Science"},{"key":"10.1016\/j.vas.2025.100445_bib0038","first-page":"3","article-title":"Measuring miRNA in livestock using sensor technologies: Challenges and potential approaches","volume":"10","author":"Neethirajan","year":"2022","journal-title":"Biology and Life Sciences Forum"},{"key":"10.1016\/j.vas.2025.100445_bib0039","first-page":"2825","article-title":"Scikit-learn: Machine learning in Python","volume":"12","author":"Pedregosa","year":"2011","journal-title":"Journal of Machine Learning Research : JMLR"},{"key":"10.1016\/j.vas.2025.100445_bib0040","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1017\/S1751731109004479","article-title":"Perceptions of the importance of different welfare issues in livestock production","volume":"3","author":"Phillips","year":"2009","journal-title":"Animal : An International Journal of Animal Bioscience"},{"key":"10.1016\/j.vas.2025.100445_bib0041","doi-asserted-by":"crossref","first-page":"3416","DOI":"10.1007\/s12035-015-9281-4","article-title":"Circulating microRNA signatures in rodent models of pain","volume":"53","author":"Qureshi","year":"2016","journal-title":"Molecular Neurobiology"},{"key":"10.1016\/j.vas.2025.100445_bib0042","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.domaniend.2015.05.005","article-title":"Twenty-four-hour profiles of metabolic and stress hormones in sheep selected for a calm or nervous temperament","volume":"53","author":"Rietema","year":"2015","journal-title":"Domestic Animal Endocrinology"},{"key":"10.1016\/j.vas.2025.100445_bib0043","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/9781118999196.ch31","article-title":"Recognition and assessment of pain in ruminants","author":"Stafford","year":"2013","journal-title":"Pain Management in Veterinary Practice"},{"key":"10.1016\/j.vas.2025.100445_bib0044","doi-asserted-by":"crossref","first-page":"2370","DOI":"10.1071\/AN17312","article-title":"Neurophysiological assessment of animal welfare","volume":"57","author":"Tilbrook","year":"2017","journal-title":"Animal Production Science"},{"key":"10.1016\/j.vas.2025.100445_bib0045","doi-asserted-by":"crossref","first-page":"4324","DOI":"10.1210\/en.2010-0234","article-title":"Stressor specificity of sex differences in hypothalamo-pituitary-adrenal axis activity: Cortisol responses to exercise, endotoxin, wetting, and isolation\/restraint stress in gonadectomized male and female sheep","volume":"151","author":"Turner","year":"2010","journal-title":"Endocrinology"},{"key":"10.1016\/j.vas.2025.100445_bib0046","doi-asserted-by":"crossref","first-page":"5131","DOI":"10.1038\/s41598-022-09214-9","article-title":"MiRNAs in milk can be used towards early prediction of mammary gland inflammation in cattle","volume":"12","author":"Tzelos","year":"2022","journal-title":"Scientific Reports"},{"key":"10.1016\/j.vas.2025.100445_bib0047","series-title":"The effect of predator presence on the behaviour of sheep in pain","author":"Young","year":"2006"},{"key":"10.1016\/j.vas.2025.100445_bib0048","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1159\/000526058","article-title":"Postoperative severity assessment in sheep","volume":"64","author":"Zentrich","year":"2023","journal-title":"European Surgical Research"},{"key":"10.1016\/j.vas.2025.100445_bib0049","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1007\/s12192-014-0521-8","article-title":"Identification and bioinformatics analysis of microRNAs associated with stress and immune response in serum of heat-stressed and normal Holstein cows","volume":"19","author":"Zheng","year":"2014","journal-title":"Cell Stress and Chaperones"},{"key":"10.1016\/j.vas.2025.100445_bib0050","doi-asserted-by":"crossref","first-page":"4606","DOI":"10.3168\/jds.2018-15515","article-title":"Integrative plasma proteomic and microRNA analysis of Jersey cattle in response to high-altitude hypoxia","volume":"102","author":"ZhiWei","year":"2019","journal-title":"Journal of Dairy Science"}],"container-title":["Veterinary and Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451943X25000225?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451943X25000225?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T04:15:16Z","timestamp":1747282516000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2451943X25000225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":49,"alternative-id":["S2451943X25000225"],"URL":"https:\/\/doi.org\/10.1016\/j.vas.2025.100445","relation":{},"ISSN":["2451-943X"],"issn-type":[{"value":"2451-943X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Circulating microRNA profiles are associated with acute pain and stress in castrated and tail docked lambs","name":"articletitle","label":"Article Title"},{"value":"Veterinary and Animal Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vas.2025.100445","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"Crown Copyright \u00a9 2025 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100445"}}