{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T12:08:11Z","timestamp":1784117291744,"version":"3.55.0"},"reference-count":39,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T00:00:00Z","timestamp":1761436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2017YFD05009"],"award-info":[{"award-number":["2017YFD05009"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary and Animal Science"],"published-print":{"date-parts":[[2025,12]]},"DOI":"10.1016\/j.vas.2025.100530","type":"journal-article","created":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:56:55Z","timestamp":1761519415000},"page":"100530","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":2,"special_numbering":"C","title":["Differentiating infected from vaccinated animals: Validation and application of NH-AGID assay in heifers immunized with Brucella abortus strain A19-\u2206VirB12"],"prefix":"10.1016","volume":"30","author":[{"given":"Feng","family":"Ye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-jing","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-ya","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-xi","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-liang","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-rui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-ping","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"issue":"1\u20132","key":"10.1016\/j.vas.2025.100530_bib0001","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0378-1135(99)00141-8","article-title":"Evaluation of three Brucella soluble antigens used in an indirect Elisa to discriminate S19 vaccinated from naturally infected cattle","volume":"71","author":"Abalos","year":"2000","journal-title":"Veterinary Microbiology"},{"issue":"7","key":"10.1016\/j.vas.2025.100530_bib0002","first-page":"748","article-title":"Detection of Brucella abortus Vaccine Strain RB51 in Water Buffalo (Bubalus bubalis) Milk","volume":"11","author":"Averaimo","year":"2022","journal-title":"Pathogens (Basel, Switzerland)"},{"issue":"1","key":"10.1016\/j.vas.2025.100530_bib0003","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13567-017-0422-9","article-title":"Characterization of Brucella abortus mutant strain \u039422915, a potential vaccine candidate","volume":"48","author":"Bao","year":"2017","journal-title":"Veterinary Research"},{"issue":"8","key":"10.1016\/j.vas.2025.100530_bib0004","doi-asserted-by":"crossref","first-page":"1835","DOI":"10.1128\/jcm.32.8.1835-1840.1994","article-title":"Evaluation of allergic and serological tests for diagnosing Brucella melitensis infection in sheep","volume":"32","author":"Blasco","year":"1994","journal-title":"Journal of Clinical Microbiology"},{"issue":"4","key":"10.1016\/j.vas.2025.100530_bib0005","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1111\/tbed.14440","article-title":"Efficacy of Brucella abortus S19 and RB51 vaccine strains: A systematic review and meta-analysis","volume":"69","author":"Blasco","year":"2022","journal-title":"Transboundary and Emerging Diseases"},{"issue":"3","key":"10.1016\/j.vas.2025.100530_bib0006","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1016\/j.vaccine.2020.11.033","article-title":"Evaluation of the safety profile of the vaccine candidate Brucella melitensis 16M\u0394vjbR strain in goats","volume":"39","author":"Casta\u00f1o-Zubieta","year":"2021","journal-title":"Vaccine"},{"issue":"11","key":"10.1016\/j.vas.2025.100530_bib0007","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0260288","article-title":"Brucella abortus S19 GFP-tagged vaccine allows the serological identification of vaccinated cattle","volume":"16","author":"Chac\u00f3n-D\u00edaz","year":"2021","journal-title":"PloS one"},{"issue":"12","key":"10.1016\/j.vas.2025.100530_bib0008","doi-asserted-by":"crossref","DOI":"10.3390\/vaccines9121423","article-title":"Immunization with Brucella abortus S19\u0394per Conferred Protection in Water Buffaloes against Virulent Challenge with B. abortus Strain S544","volume":"9","author":"Chaudhuri","year":"2021","journal-title":"Vaccines"},{"key":"10.1016\/j.vas.2025.100530_bib0009","unstructured":"China Statistical Yearbook(2021). (2021)."},{"key":"10.1016\/j.vas.2025.100530_bib0010","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.vetimm.2018.02.010","article-title":"Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis REV1 vaccine produced in bioreactor","volume":"198","author":"Curina","year":"2018","journal-title":"Veterinary Immunology and Immunopathology"},{"issue":"1","key":"10.1016\/j.vas.2025.100530_bib0011","first-page":"51","article-title":"Identification of Main Brucella species Implicated in Ovine and Caprine Abortion Cases by Molecular and Classical Methods","volume":"76","author":"Dadar","year":"2021","journal-title":"Archives of Razi Institute"},{"issue":"1","key":"10.1016\/j.vas.2025.100530_bib0012","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1186\/s40249-021-00834-3","article-title":"Fluorescence polarization assay improves the rapid detection of human brucellosis in China","volume":"10","author":"Dong","year":"2021","journal-title":"Infectious Diseases of Poverty"},{"issue":"3","key":"10.1016\/j.vas.2025.100530_bib0013","first-page":"e9394","article-title":"Shedding Rates and SeroPrevalence of Brucella melitensis in Lactating Goats of Shahrekord, Iran","volume":"7","author":"Ebrahimi","year":"2014","journal-title":"Jundishapur Journal of Microbiology"},{"issue":"5","key":"10.1016\/j.vas.2025.100530_bib0014","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1177\/1040638720943880","article-title":"Comparison of 2 ELISAs for detecting exposure to Brucella ovis","volume":"32","author":"Elderbrook","year":"2020","journal-title":"Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc"},{"issue":"9","key":"10.1016\/j.vas.2025.100530_bib0015","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1292\/jvms.64.839","article-title":"Serological differentiation of Brucella-vaccinated and -infected domesticated animals by the agar gel immunodiffusion test using Brucella polysaccharide in mongolia","volume":"64","author":"Erdenebaatar","year":"2002","journal-title":"The Journal of Veterinary Medical Science"},{"issue":"1","key":"10.1016\/j.vas.2025.100530_bib0016","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1128\/jcm.24.1.76-80.1986","article-title":"Demonstration of antibodies against Brucella melitensis 16M lipopolysaccharide and native hapten in human sera by enzyme-linked immunosorbent assay","volume":"24","author":"Fernandex-Lago","year":"1986","journal-title":"Journal of Clinical Microbiology"},{"issue":"3","key":"10.1016\/j.vas.2025.100530_bib0017","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s11250-023-03635-y","article-title":"Economic analysis of bovine brucellosis control in the Rond\u00f4nia state, Brazil","volume":"55","author":"Ferreira","year":"2023","journal-title":"Tropical Animal Health and Production"},{"issue":"1","key":"10.1016\/j.vas.2025.100530_bib0018","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1309\/LM4J9MWOBIPA6RBN","article-title":"Comparison of methods for diagnosing brucellosis","volume":"44","author":"Hajia","year":"2013","journal-title":"Laboratory Medicine"},{"key":"10.1016\/j.vas.2025.100530_bib0019","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2022.925773","article-title":"Evaluation of Brucellosis Vaccines: A Comprehensive Review","volume":"9","author":"Heidary","year":"2022","journal-title":"Frontiers in Veterinary Science"},{"issue":"6","key":"10.1016\/j.vas.2025.100530_bib0020","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1128\/jcm.12.6.753-760.1980","article-title":"Evaluation of a radial immunodiffusion test with polysaccharide B antigen for diagnosis of bovine brucellosis","volume":"12","author":"Jones","year":"1980","journal-title":"Journal of Clinical Microbiology"},{"issue":"3","key":"10.1016\/j.vas.2025.100530_bib0021","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1007\/s11250-022-03175-x","article-title":"Molecular detection of Brucella spp. in ruminant herds in Greece","volume":"54","author":"Katsiolis","year":"2022","journal-title":"Tropical Animal Health and Production"},{"issue":"1","key":"10.1016\/j.vas.2025.100530_bib0022","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.rvsc.2008.05.012","article-title":"Comparison of agar gel immunodiffusion test, rapid slide agglutination test, microbiological culture and PCR for the diagnosis of canine brucellosis","volume":"86","author":"Keid","year":"2009","journal-title":"Research in Veterinary Science"},{"issue":"8","key":"10.1016\/j.vas.2025.100530_bib0023","first-page":"10","article-title":"Assays for Identification and Differentiation of Brucella Species: A Review","author":"Kurmanov","year":"2022","journal-title":"Microorganisms"},{"issue":"S1","key":"10.1016\/j.vas.2025.100530_bib0024","doi-asserted-by":"crossref","first-page":"281","DOI":"10.4142\/jvs.2017.18.S1.281","article-title":"Development and trial of vaccines against Brucella","volume":"18","author":"Lalsiamthara","year":"2017","journal-title":"Journal of Veterinary Science"},{"issue":"7","key":"10.1016\/j.vas.2025.100530_bib0025","first-page":"638","article-title":"Establishment of a dual real-time fluorescent PCR method for detecting the Brucella abortus A19-\u25b3VirB12 mutant vaccine strain","volume":"38","author":"Ma","year":"2022","journal-title":"Chinese Journal of Zoonoses"},{"issue":"2","key":"10.1016\/j.vas.2025.100530_bib0026","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1128\/CDLI.6.2.269-272.1999","article-title":"Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis","volume":"6","author":"Mar\u00edn","year":"1999","journal-title":"Clinical and Diagnostic Laboratory Immunology"},{"key":"10.1016\/j.vas.2025.100530_bib0028","doi-asserted-by":"crossref","DOI":"10.3389\/fvets.2022.1017851","article-title":"Molecular characterization of Brucella spp. from seropositive herds of cattle farmed at the wildlife-livestock-human interface in Rwanda","volume":"9","author":"Ntivuguruzwa","year":"2022","journal-title":"Frontiers in Veterinary Science"},{"issue":"2","key":"10.1016\/j.vas.2025.100530_bib0029","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human Brucellosis","volume":"6","author":"Pappas","year":"2006","journal-title":"The Lancet Infectious Diseases"},{"issue":"6","key":"10.1016\/j.vas.2025.100530_bib0030","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1128\/CVI.00046-08","article-title":"Use of the Brucella melitensis native hapten to diagnose brucellosis in goats by a rapid, simple, and specific fluorescence polarization assay","volume":"15","author":"Ram\u00edrez-Pfeiffer","year":"2008","journal-title":"Clinical and Vaccine Immunology : CVI"},{"issue":"2","key":"10.1016\/j.vas.2025.100530_bib0031","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1128\/CVI.00374-07","article-title":"VirB12 is a serological marker of Brucella infection in experimental and natural hosts","volume":"15","author":"Rol\u00e1n","year":"2008","journal-title":"Clinical and Vaccine Immunology : CVI"},{"issue":"3\u20134","key":"10.1016\/j.vas.2025.100530_bib0032","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/S0167-5877(00)00130-6","article-title":"Use of Brucella abortus vaccine strain RB51 in pregnant cows after calfhood vaccination with strain 19 in Argentina","volume":"45","author":"Samartino","year":"2000","journal-title":"Preventive Veterinary Medicine"},{"key":"10.1016\/j.vas.2025.100530_bib0033","unstructured":"Serological detection method of bovine brucellosis A19 delta VirB12 vaccine (Nederland Patent No. 2031068).. https:\/\/mijnoctrooi.rvo.nl\/fo-eregister-view\/search\/details\/2031068_NP\/0\/0\/1\/10\/0\/0\/0\/null_en_null\/KG51bW1lcjooMjAzMTA2OCkpIEFORCBwYXRlbnRSZWNvcmRTZXE6MQ==. 2022."},{"key":"10.1016\/j.vas.2025.100530_bib0034","doi-asserted-by":"crossref","DOI":"10.1016\/j.jns.2020.117280","article-title":"Brucellosis","volume":"420","author":"Shakir","year":"2021","journal-title":"Journal of the Neurological Sciences"},{"issue":"12","key":"10.1016\/j.vas.2025.100530_bib0035","first-page":"13","article-title":"Detection and Molecular Diversity of Brucella melitensis in Pastoral Livestock in North-Eastern Ethiopia","author":"Sibhat","year":"2024","journal-title":"Pathogens (Basel, Switzerland)"},{"key":"10.1016\/j.vas.2025.100530_bib0036","doi-asserted-by":"crossref","DOI":"10.1016\/j.cimid.2020.101462","article-title":"Characterization of the main immunogenic proteins in Brucella infection for their application in diagnosis of brucellosis","volume":"70","author":"Tian","year":"2020","journal-title":"Comparative Immunology, Microbiology and Infectious Diseases"},{"key":"10.1016\/j.vas.2025.100530_bib0037","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.molimm.2021.02.006","article-title":"Development of Brucella melitensis Rev.1 \u0394Omp19 mutants with DIVA feature and comparison of their efficacy against three commercial vaccines in a mouse model","volume":"133","author":"Uslu","year":"2021","journal-title":"Molecular Immunology"},{"key":"10.1016\/j.vas.2025.100530_bib0038","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1016\/j.prevetmed.2019.04.015","article-title":"Seroprevalence of Brucella canis antibodies in dogs entering a Minnesota humane society, Minnesota, 2016-2017","volume":"168","author":"Whitten","year":"2019","journal-title":"Preventive Veterinary Medicine"},{"key":"10.1016\/j.vas.2025.100530_bib0040","article-title":"Evaluation and Differential Diagnosis of a Genetic Marked Brucella Vaccine A19\u0394virB12 for Cattle","volume":"12","author":"Yang","year":"2021","journal-title":"Frontiers in Immunology"},{"issue":"11","key":"10.1016\/j.vas.2025.100530_bib0041","first-page":"1213","article-title":"Construction of Brucella abortus A19-\u0394VirB12 mutant and evaluation of its protective efficacy against 2308 strain challenge in BALB\/c mice","volume":"53","author":"Yi","year":"2013","journal-title":"Acta Microbiologica Sinica"}],"container-title":["Veterinary and Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451943X25001024?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451943X25001024?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T15:40:09Z","timestamp":1774885209000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2451943X25001024"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":39,"alternative-id":["S2451943X25001024"],"URL":"https:\/\/doi.org\/10.1016\/j.vas.2025.100530","relation":{},"ISSN":["2451-943X"],"issn-type":[{"value":"2451-943X","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"Differentiating infected from vaccinated animals: Validation and application of NH-AGID assay in heifers immunized with Brucella abortus strain A19-\u2206VirB12","name":"articletitle","label":"Article Title"},{"value":"Veterinary and Animal Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vas.2025.100530","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2025 The Authors. Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100530"}}