{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:36:40Z","timestamp":1793216200837,"version":"4.5.0"},"reference-count":47,"publisher":"Elsevier BV","license":[{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/tdm\/userlicense\/1.0\/"},{"start":{"date-parts":[[2026,12,1]],"date-time":"2026-12-01T00:00:00Z","timestamp":1796083200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.elsevier.com\/legal\/tdmrep-license"},{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["elsevier.com","sciencedirect.com"],"crossmark-restriction":true},"short-container-title":["Veterinary and Animal Science"],"published-print":{"date-parts":[[2026,12]]},"DOI":"10.1016\/j.vas.2026.100787","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T23:32:39Z","timestamp":1785195159000},"page":"100787","update-policy":"https:\/\/doi.org\/10.1016\/elsevier_cm_policy","source":"Crossref","is-referenced-by-count":0,"special_numbering":"C","title":["The effect of increasing dietary Vitamin E and D doses on the performance of broilers and CLDN2 gene expression during experimental heat stress"],"prefix":"10.1016","volume":"34","author":[{"given":"Soudeh Shaabanzadeh","family":"Soufiabadi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Chamani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Asghar","family":"Sadeghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Aminafshar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farhad","family":"Foroudi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"78","reference":[{"key":"10.1016\/j.vas.2026.100787_bib0001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40104-016-0097-5","article-title":"Association between heat stress and oxidative stress in poultry; mitochondrial dysfunction and dietary interventions with phytochemicals","volume":"7","author":"Akbarian","year":"2016","journal-title":"Journal Of Animal Science And Biotechnology"},{"key":"10.1016\/j.vas.2026.100787_bib0003","first-page":"1","article-title":"Effects of free and nanoencapsulated garlic essential oil on growth performance and immune functions in broiler chickens","author":"Amiri","year":"2024","journal-title":"Animal Science Journal"},{"key":"10.1016\/j.vas.2026.100787_bib0004","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1515\/aoas-2017-0012","article-title":"Enhancing tolerance of broiler chickens to heat stress by supplementation with vitamin E, vitamin C and\/or probiotics","volume":"17","author":"Attia","year":"2017","journal-title":"Annals of Animal Science"},{"key":"10.1016\/j.vas.2026.100787_bib0005","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2022.101748","article-title":"Influence of incubation, diet, and sex on avian uncoupling protein expression and oxidative stress in market age broilers following exposure to acute heat stress","volume":"101","author":"Brannan","year":"2022","journal-title":"Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0006","doi-asserted-by":"crossref","first-page":"4767","DOI":"10.3382\/ps\/pez192","article-title":"Dietary mannan oligosaccharide ameliorates cyclic heat stress-induced damages on intestinal oxidative status and barrier integrity of broilers","volume":"98","author":"Cheng","year":"2019","journal-title":"Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0007","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1007\/s00384-020-03576-0","article-title":"Vitamin D regulates CLDN2 and CLDN4 expression in active ulcerative colitis by p-stat-6 and Smad-7 signaling","volume":"35","author":"Domazetovic","year":"2020","journal-title":"International Journal Of Colorectal Disease"},{"key":"10.1016\/j.vas.2026.100787_bib0008","doi-asserted-by":"crossref","first-page":"3787","DOI":"10.3382\/ps\/pez082","article-title":"Influence of feed particle size and feed form on productive performance, egg quality, gastrointestinal tract traits, digestive enzymes, intestinal morphology, and nutrient digestibility of laying hens reared in enriched cages","volume":"98","author":"Ege","year":"2019","journal-title":"Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0009","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3382\/ps\/pex298","article-title":"Effects of dietary vitamin C, vitamin E, and alpha-lipoic acid supplementation on the antioxidant defense system and immune-related gene expression in broilers exposed to oxidative stress by dexamethasone","volume":"97","author":"El-Senousey","year":"2018","journal-title":"Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0010","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1007\/s12011-012-9374-0","article-title":"Effects of dietary selenium, vitamin E, and their combination on growth, serum metabolites, and antioxidant defense system in skeletal muscle of broilers under heat stress","volume":"148","author":"Ghazi Harsini","year":"2012","journal-title":"Biological Trace Element Research"},{"key":"10.1016\/j.vas.2026.100787_bib0011","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1007\/s00484-014-0915-4","article-title":"Single and combined effects of vitamin C and oregano essential oil in diet, on growth performance, and blood parameters of broiler chicks reared under heat stress condition","volume":"59","author":"Ghazi","year":"2015","journal-title":"International Journal Of Biometeorology"},{"key":"10.1016\/j.vas.2026.100787_bib0012","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s40104-020-00523-5","article-title":"Regulation of gene expression in chickens by heat stress","volume":"12","author":"Goel","year":"2021","journal-title":"Journal Of Animal Science And Biotechnology"},{"key":"10.1016\/j.vas.2026.100787_bib0013","doi-asserted-by":"crossref","first-page":"107","DOI":"10.3390\/ani9030107","article-title":"Effect of heat stress and stocking density on growth performance, breast meat quality, and intestinal barrier function in broiler chickens","volume":"9","author":"Goo","year":"2019","journal-title":"Animals"},{"key":"10.1016\/j.vas.2026.100787_bib0014","doi-asserted-by":"crossref","first-page":"1156","DOI":"10.5713\/ajas.19.0274","article-title":"Effects of compound organic acid calcium on growth performance, hepatic antioxidation and intestinal barrier of male broilers under heat stress","volume":"33","author":"He","year":"2020","journal-title":"Asian-Australasian Journal of Animal Sciences"},{"issue":"2","key":"10.1016\/j.vas.2026.100787_bib0015","doi-asserted-by":"crossref","first-page":"103","DOI":"10.2478\/s10146-009-0012-1","article-title":"Influence of dietary vitamin E and zinc on performance, oxidative stability and some blood measures of broiler chickens reared under heat stress (35 \u00b0C)","volume":"27","author":"Hosseini-Mansoub","year":"2010","journal-title":"Journal of Agrobiology"},{"key":"10.1016\/j.vas.2026.100787_bib0016","doi-asserted-by":"crossref","first-page":"67","DOI":"10.3390\/antiox8030067","article-title":"Polyphenols as potential attenuators of heat stress in poultry production","volume":"8","author":"Hu","year":"2019","journal-title":"Antioxidants"},{"key":"10.1016\/j.vas.2026.100787_bib0017","first-page":"4534","article-title":"Effects of Bacillus coagulans on performance, egg quality and plasma biochemical parameters of laying hens infected with Salmonella","volume":"29","author":"Huang","year":"2017","journal-title":"Chinese Journal of Animal Nutrition"},{"key":"10.1016\/j.vas.2026.100787_bib0018","doi-asserted-by":"crossref","first-page":"749","DOI":"10.5713\/ajas.2013.13852","article-title":"Effects of vitamin C or E on the pro-inflammatory cytokines, heat shock protein 70 and antioxidant status in broiler chicks under summer conditions","volume":"27","author":"Jang","year":"2014","journal-title":"Asian-Australasian Journal of Animal Sciences"},{"issue":"9","key":"10.1016\/j.vas.2026.100787_bib0019","doi-asserted-by":"crossref","first-page":"3229","DOI":"10.3382\/ps\/pex099","article-title":"The effect of DL-, L-isomers and DL-hydroxy analog administered at 2 levels as dietary sources of methionine on the metabolic and antioxidant parameters and growth performance of turkeys","volume":"96","author":"Jankowski","year":"2017","journal-title":"Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0020","doi-asserted-by":"crossref","first-page":"469","DOI":"10.1017\/S0043933911000511","article-title":"Effect of vitamin E in heat-stressed poultry","volume":"67","author":"Khan","year":"2011","journal-title":"World's Poultry Science Journal"},{"key":"10.1016\/j.vas.2026.100787_bib0021","doi-asserted-by":"crossref","first-page":"99","DOI":"10.2174\/1573401311309020004","article-title":"Protection and restitution of gut barrier by probiotics: Nutritional and clinical implications","volume":"9","author":"Krishna Rao","year":"2013","journal-title":"Current Nutrition And Food Science"},{"key":"10.1016\/j.vas.2026.100787_bib0023","doi-asserted-by":"crossref","first-page":"734","DOI":"10.3390\/nu12030734","article-title":"Beyond heat stress: Intestinal integrity disruption and mechanism-based intervention strategies","volume":"12","author":"Lian","year":"2020","journal-title":"Nutrients"},{"key":"10.1016\/j.vas.2026.100787_bib0024","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1079\/WPS200585","article-title":"Strategies for preventing heat stress in poultry","volume":"62","author":"Lin","year":"2006","journal-title":"World's Poultry Science Journal"},{"key":"10.1016\/j.vas.2026.100787_bib0025","article-title":"Microbial products induce CLDN2 to compromise gut epithelial barrier function","volume":"8","author":"Liu","year":"2013","journal-title":"PLoS One"},{"key":"10.1016\/j.vas.2026.100787_bib0028","first-page":"107","article-title":"Effect of long-term heat stress on key enzyme activities and T3 levels in commercial layer hens","volume":"2","author":"Melesse","year":"2011","journal-title":"International Journal of Livestock Production"},{"key":"10.1016\/j.vas.2026.100787_bib0029","doi-asserted-by":"crossref","first-page":"1101","DOI":"10.3382\/ps\/pex421","article-title":"Effect of dietary synbiotic supplement on behavioral patterns and growth performance of broiler chickens reared under heat stress","volume":"97","author":"Mohammed","year":"2018","journal-title":"Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0031","doi-asserted-by":"crossref","first-page":"2101","DOI":"10.3382\/ps.2009-00220","article-title":"Effects of different levels of vitamin E on growth performance and immune responses of broilers under heat stress","volume":"88","author":"Niu","year":"2009","journal-title":"Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0032","unstructured":"Oikeh, I. Coccidiosis in modern broiler chickens: Targeted nutritional modulations for consequences on bone quality 2019."},{"key":"10.1016\/j.vas.2026.100787_bib0033","doi-asserted-by":"crossref","first-page":"109","DOI":"10.2141\/jpsa.0130134","article-title":"Role of vitamin E in counteracting oxidative stress in poultry","volume":"51","author":"Panda","year":"2014","journal-title":"The Journal Of Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0034","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1016\/j.ajpath.2011.10.025","article-title":"Probiotic bacteria induce maturation of intestinal CLDN3 expression and barrier function","volume":"180","author":"Patel","year":"2012","journal-title":"The American Journal Of Pathology"},{"issue":"2","key":"10.1016\/j.vas.2026.100787_bib0035","first-page":"169","article-title":"Effect of probiotic and different sources of fat on performance, carcass characteristics, intestinal morphology and ghrelin gene expression on broiler chickens","volume":"24","author":"Poorghasemi","year":"2017","journal-title":"Kafkas \u00dcniversitesi Veteriner Fak\u00fcltesi Dergisi"},{"issue":"5","key":"10.1016\/j.vas.2026.100787_bib0036","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1002\/lipd.12397","article-title":"Effects of different lipid sources with or without a probiotic on gastrointestinal tract, immune system and blood parameters of chickens: An animal model","volume":"59","author":"Poorghasemi","year":"2024","journal-title":"Lipids"},{"key":"10.1016\/j.vas.2026.100787_bib0037","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1093\/ps\/80.8.1190","article-title":"Effects of vitamin E and C supplementation on performance, in vitro lymphocyte proliferation, and antioxidant status of laying hens during heat stress","volume":"80","author":"Puthpongsiriporn","year":"2001","journal-title":"Poultry Science"},{"issue":"5","key":"10.1016\/j.vas.2026.100787_bib0038","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1111\/jpn.12457","article-title":"Effect of lemon verbena powder and vitamin C on performance and immunity of heat stressed broilers","volume":"100","author":"Rafiee","year":"2016","journal-title":"Journal of Animal Physiology and Animal Nutrition"},{"key":"10.1016\/j.vas.2026.100787_bib0039","doi-asserted-by":"crossref","DOI":"10.1155\/2018\/5123147","article-title":"Oxidative stress in poultry: Lessons from the viral infections","volume":"2018","author":"Rehman","year":"2018","journal-title":"Oxidative Medicine And Cellular Longevity"},{"key":"10.1016\/j.vas.2026.100787_bib0041","first-page":"244","article-title":"Vitamin C and E can alleviate negative effects of heat stress in Japanese quails","volume":"1","author":"Sahin","year":"2003","journal-title":"Food, Agriculture and Environment"},{"key":"10.1016\/j.vas.2026.100787_bib0042","doi-asserted-by":"crossref","first-page":"584","DOI":"10.4038\/tar.v26i4.8121","article-title":"Changes in the serum enzyme levels and liver lesions of broiler birds reared under different management conditions","volume":"26","author":"Senanayake","year":"2015","journal-title":"Tropical Agricultural Research"},{"key":"10.1016\/j.vas.2026.100787_bib0044","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.rvsc.2015.03.009","article-title":"Vitamin D3 modulates the function of chicken macrophages","volume":"100","author":"Shojadoost","year":"2015","journal-title":"Research In Veterinary Science"},{"issue":"4","key":"10.1016\/j.vas.2026.100787_bib0045","doi-asserted-by":"crossref","DOI":"10.1016\/j.psj.2020.12.027","article-title":"Centennial Review: Effects of vitamins A, D, E, and C on the chicken immune system","volume":"100","author":"Shojadoost","year":"2021","journal-title":"Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0046","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-75885-x","article-title":"Chronic heat stress regulates the relation between heat shock protein and immunity in broiler small intestine","volume":"10","author":"Siddiqui","year":"2020","journal-title":"Scientific Reports"},{"key":"10.1016\/j.vas.2026.100787_bib0048","doi-asserted-by":"crossref","first-page":"31263","DOI":"10.1074\/jbc.M111.238147","article-title":"Interleukin-6 (IL-6) regulates CLDN2 expression and tight junction permeability in intestinal epithelium","volume":"286","author":"Suzuki","year":"2011","journal-title":"The Journal Of Biological Chemistry"},{"issue":"42","key":"10.1016\/j.vas.2026.100787_bib0050","first-page":"183","article-title":"Effect of dietary extract of thyme and vitamin E supplementation on immune responses and yolk cholesterol in laying hen under heat stress condition","volume":"2","author":"Tahmasbi","year":"2012","journal-title":"Journal of Medicinal Plants"},{"key":"10.1016\/j.vas.2026.100787_bib0051","first-page":"10","article-title":"The effect of dietary supplementation of some antioxidants on performance, oxidative stress, and blood parameters in broilers under natural summer conditions","volume":"4","author":"Tawfeek","year":"2014","journal-title":"The Journal of World's Poultry Research"},{"key":"10.1016\/j.vas.2026.100787_bib0052","doi-asserted-by":"crossref","first-page":"1266","DOI":"10.3390\/ani10081266","article-title":"Impact of heat stress on poultry health and performances, and potential mitigation strategies","volume":"10","author":"Wasti","year":"2020","journal-title":"Animals"},{"key":"10.1016\/j.vas.2026.100787_bib0053","first-page":"5202","article-title":"Differentially expressed genes in vitamin D regulating calcium and phosphorus absorption in duodenum of broiler chickens using RNAsequencing","volume":"31","author":"Yang","year":"2019","journal-title":"Chinese Journal of Animal Nutrition"},{"key":"10.1016\/j.vas.2026.100787_bib0054","doi-asserted-by":"crossref","first-page":"124","DOI":"10.2141\/jpsa.0190013","article-title":"Optimal dietary levels of 1\u03b1-hydroxycholecalciferol in broiler chickens from 1 to 42 days of age","volume":"57","author":"Yang","year":"2020","journal-title":"Journal of Poultry Science"},{"key":"10.1016\/j.vas.2026.100787_bib0055","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1111\/j.1365-2362.2012.02706.x","article-title":"Vitamin D attenuates high fat diet -induced hepatic steatosis in rats by modulating lipid metabolism","volume":"42","author":"Yin","year":"2012","journal-title":"European Journal of Clinical Investigation"},{"key":"10.1016\/j.vas.2026.100787_bib0056","first-page":"1","article-title":"Tight junction CLDN2 gene is a direct target of the vitamin D receptor","volume":"5","author":"Zhang","year":"2015","journal-title":"Scientific Reports"}],"container-title":["Veterinary and Animal Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451943X26002176?httpAccept=text\/xml","content-type":"text\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.elsevier.com\/content\/article\/PII:S2451943X26002176?httpAccept=text\/plain","content-type":"text\/plain","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T19:30:44Z","timestamp":1793215844000},"score":1,"resource":{"primary":{"URL":"https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/S2451943X26002176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,12]]},"references-count":47,"alternative-id":["S2451943X26002176"],"URL":"https:\/\/doi.org\/10.1016\/j.vas.2026.100787","relation":{},"ISSN":["2451-943X"],"issn-type":[{"value":"2451-943X","type":"print"}],"subject":[],"published":{"date-parts":[[2026,12]]},"assertion":[{"value":"Elsevier","name":"publisher","label":"This article is maintained by"},{"value":"The effect of increasing dietary Vitamin E and D doses on the performance of broilers and CLDN2 gene expression during experimental heat stress","name":"articletitle","label":"Article Title"},{"value":"Veterinary and Animal Science","name":"journaltitle","label":"Journal Title"},{"value":"https:\/\/doi.org\/10.1016\/j.vas.2026.100787","name":"articlelink","label":"CrossRef DOI link to publisher maintained version"},{"value":"article","name":"content_type","label":"Content Type"},{"value":"\u00a9 2026 Published by Elsevier Ltd.","name":"copyright","label":"Copyright"}],"article-number":"100787"}}